Abstract：
:Stem cell proliferation induced by potent cytokines usually leads to a loss of primitive potential through differentiation. In this study, the ability of cytokines and murine MS5 stromal cells to independently regulate the proliferation and long-term culture-initiating cell (LTC-IC) activity of primitive CD34(+)CD38(low/neg) human bone marrow cells was evaluated. To compare populations with identical proliferation histories, cells were labeled with carboxy fluorescein diacetate succinimidyl ester, and LTC-IC activity was assessed 4 days later in cells that had accomplished the same number of divisions with or without MS5 cells. MS5 cells counteracted dramatically the loss of LTC-IC activity observed in the presence of cytokines alone. Thus, in the presence of MS5 cells, means of 1233 (n = 5) and 355 (n = 9) LTC-IC-derived colony-forming cells (CFCs) were generated by 1000 cells that performed 3 and 4 divisions respectively, whereas 311 (n = 5) and 64 (n = 5) CFCs were generated by 1000 cells cultured without MS5 cells. Interestingly, MS5 cells had no detectable effect on the LTC-IC activity of cells that divided only twice in 4 days-1606 CFCs (n = 6) and 1993 (n = 6) CFCs, respectively, without and with MS5 cells-and a 48 additional hours of coculture were necessary to unmask changes in the LTC-IC activity mediated by stromal cells. These results indicate that cytokines and stroma-derived signals can regulate independently the proliferation and differentiation of primitive cells and that these stroma-derived extracellular factors act directly on their target cells.
journal_name
Bloodjournal_title
Bloodauthors
Bennaceur-Griscelli A,Pondarré C,Schiavon V,Vainchenker W,Coulombel Ldoi
10.1182/blood.v97.2.435subject
Has Abstractpub_date
2001-01-15 00:00:00pages
435-41issue
2eissn
0006-4971issn
1528-0020journal_volume
97pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Twenty consecutive patients with Hodgkin disease in continuous complete remission and off treatment for at least 5 yr (range 5-25 yr, median 9 yr) were studied with a battery of immunologic parameters. Skin test reactivity to four common antigens, sensitization to 2,4-dinitrochlorobenzene, absolute lymphocyte count, r...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Aging is associated with a decline in B-lymphopoiesis in the bone marrow and accumulation of long-lived B cells in the periphery. These changes decrease the body's ability to mount protective antibody responses. We show here that age-related changes in the B lineage are mediated by the accumulating long-lived B cells....
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-09-307983
更新日期：2011-03-17 00:00:00
abstract：:Angiotensin I-converting enzyme (ACE) inhibitors can affect hematopoiesis by several mechanisms including inhibition of angiotensin II formation and increasing plasma concentrations of AcSDKP (acetyl-N-Ser-Asp-Lys-Pro), an ACE substrate and a negative regulator of hematopoiesis. We tested whether ACE inhibition could ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-11-3828
更新日期：2004-08-15 00:00:00
abstract：:Notch and its ligands have been implicated in the regulation and differentiation of various CD4(+) T-helper cells. Regulatory T cells (T(regs)), which express the transcription factor Foxp3, suppress aberrant immune responses that are typically associated with autoimmunity or excessive inflammation. Previous studies h...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-03-144980
更新日期：2008-09-01 00:00:00
abstract：:Human platelets contain tortuous channels in their cytoplasm, the surface-connected or open canalicular system (OCS), that communicate directly with the surrounding medium through openings on the surface membrane. Some workers have suggested that the OCS serves as the egress route for products secreted during the rele...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:Seventy-five patients with resistant acute leukemia or lymphoma received high-dose cyclophosphamide and etoposide to explore the activity of this combination in resistant hematologic malignancies, and to determine the maximum doses of these drugs that can be combined without bone marrow transplantation. Etoposide was ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:The malignant Reed-Sternberg cell of Hodgkin disease is an aberrant B cell that persists in an immunolgically mediated inflammatory infiltrate. Despite its nonproductive immunoglobulin genes, the Reed-Sternberg cell avoids the usual apoptotic fate of defective immune cells through an unknown mechanism. A likely candid...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-10-15 00:00:00
abstract：:Localized tumors composed of immature cells of the myelogenous series have been recognized for many years as an uncommon manifestation of granulocytic leukemia. The histologic diagnosis of chloroma (granulocytic sarcoma) may be extremely difficult when the myeloblastic cells are poorly differentiated and the tumor lac...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：:The lymphocytosis manifested in infectious mononucleosis (IM) during acute phase is ascribed to a reactive expansion of CD8+ T lymphocytes caused by Epstein-Barr virus (EBV)-infected B lymphocytes. Expression of HLA-DR antigen on IM lymphocytes suggests that these T lymphocytes are somehow activated in vivo. In the pr...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-01-15 00:00:00
abstract：:Proteolytic fragments of fibronectin (Fn) can possess properties not inherent to intact Fn. Previously, only mixtures of low molecular weight Fn fragments, and the 120-Kd fibroblastic cell-binding segment, but not intact Fn, were shown to be selectively chemotactic for human monocytes (MOs). In order to determine if o...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-11-15 00:00:00
abstract：:von Willebrand's antigen II (vW AgII), a plasma and platelet antigen immunologically and biochemically distinct from factor-VIII-related antigen (VIIIR:Ag), is decreased in von Willebrand's disease. vW AgII is released from platelets during aggregation and clotting. The release of platelet vW AgII was studied in washe...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：:We report here the results of polymerase chain reaction (PCR) for bcr-abl transcript and clinical details derived from 64 chronic myelogenous leukemia (CML) patients after allogeneic bone marrow transplantation (BMT). A total of 139 samples (2 to 220 weeks after BMT) were analyzed and bcr-abl transcript was detected i...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-02-15 00:00:00
abstract：:Nonresponse to remission-induction chemotherapy, which remains a major problem in acute myeloblastic leukemia (AML), has been linked to cellular resistance to apoptosis. Because the apoptosis induced by chemotherapeutic drugs is mediated by loss of mitochondrial transmembrane potential (MTP), it was postulated that se...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.v98.2.405
更新日期：2001-07-15 00:00:00
abstract：:Bcl-2 protein expression has been associated with poor prognosis in patients with noncutaneous diffuse large B-cell lymphomas. In primary cutaneous large B-cell lymphomas, the location on the leg, the round-cell morphology defined as the predominance of centroblasts and immunoblasts over large centrocytes, and multipl...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：10.1182/blood-2003-08-2726
更新日期：2004-05-15 00:00:00
abstract：:The CD28 family of receptors (CD28, cytotoxic T-lymphocyte-associated antigen 4 [CTLA-4], inducible costimulator [ICOS], program death-1 [PD-1], and B- and T-lymphocyte attenuator [BTLA]) plays a critical role in controlling the adaptive arm of the immune response. While considerable information is available regarding...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2004-04-1596
更新日期：2005-01-01 00:00:00
abstract：:Serum response factor (SRF) is a ubiquitously expressed transcription factor and master regulator of the actin cytoskeleton. We have previously shown that SRF is essential for megakaryocyte maturation and platelet formation and function. Here we elucidate the role of SRF in neutrophils, the primary defense against inf...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2013-06-507582
更新日期：2014-05-08 00:00:00
abstract：:T cell-depleted haploidentical hematopoietic stem cell transplantation (haploHSCT) is an option to treat children with very high-risk acute lymphoblastic leukemia (ALL) lacking an HLA-identical donor. We analyzed 127 children with ALL who underwent haploHSCT in first (n = 22), second (n = 48), or third (n = 32), compl...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1182/blood-2009-03-207001
更新日期：2010-04-29 00:00:00
abstract：:The primate erythrocyte (E) complement receptor, CR1, is a transmembrane glycoprotein located in clusters on the surface of E. In vivo studies have demonstrated that during processing and clearance of complement-opsonized immune complexes, large amounts of immunoglobulin G (IgG) can be bound to primate E via CR1 with ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:High-dose chemoradiotherapy (HDT) with autologous stem cell transplantation (ASCT) is the treatment of choice for patients with relapsed aggressive non-Hodgkin lymphoma (NHL). However, its role in the treatment of patients with primary refractory disease is not well defined. The outcomes of 85 patients with primary re...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:T(11;18)(q21;q21) is the most common structural abnormality in extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) leading to the fusion of the apoptosis inhibitor-2 (API2) gene and the MALT lymphoma-associated translocation (MALT1) gene. In 2 patients with MALT lymphoma of th...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2002-09-2963
更新日期：2003-03-15 00:00:00
abstract：:Gemtuzumab ozogamicin (GO) contains an anti-CD33 antibody to facilitate uptake of a toxic calicheamicin-gamma(1) derivative. While recent in vitro data demonstrated a quantitative relationship between CD33 expression and GO cytotoxicity, previous correlative studies failed to identify a significant association between...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-09-047399
更新日期：2007-05-15 00:00:00
abstract：:A monoclonal antibody (MoAb) (LYP18), generated against human platelet glycoprotein IIb/IIIa (GPIIb/IIIa), immuno-precipitated a IIb/IIIa-like GP complex from a highly tumorigenic human melanoma cell line (M3Dau). The M3Dau melanoma cells specifically bound 125I-labeled LYP18. To study the biologic role of these IIb/I...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-08-15 00:00:00
abstract：:We conducted a genome-wide association study to identify novel associations between genetic variants and circulating plasminogen activator inhibitor-1 (PAI-1) concentration, and examined functional implications of variants and genes that were discovered. A discovery meta-analysis was performed in 19 599 subjects, foll...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-06-436188
更新日期：2012-12-06 00:00:00
abstract：:Interleukin 21 (IL-21) is a newly described cytokine with homology to IL-4 and IL-15. They belong to a cytokine family that uses the common gamma chain for signaling but also have their private high-affinity receptors. Since it is well known that IL-4 modulates differentiation and activation of dendritic cells (DCs), ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-03-0669
更新日期：2003-12-01 00:00:00
abstract：:Phosphoglycerate kinase (PGK) deficiency is generally associated with chronic hemolytic anemia, although it can be accompanied by either mental retardation or muscular disease. Genomic DNAs of two PGK-deficient patients previously described in France were sequenced directly after polymerase chain reaction amplificatio...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:The reactivation of human cytomegalovirus (HCMV) poses a serious health threat to immune compromised individuals. As a treatment strategy, dendritic cell (DC) vaccination trials are ongoing. Recent work suggests that BDCA-3(+) (CD141(+)) subset DCs may be particularly effective in DC vaccination trials. BDCA-3(+) DCs ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-06-434498
更新日期：2012-12-20 00:00:00
abstract：:Our previous study demonstrated that 2-methoxyestradiol (2ME2), an estrogen derivative, induces apoptosis in multiple myeloma (MM) cells; however, the related transcriptional events are unclear. In the present study, we used oligonucleotide microarrays to identify genes altered during 2ME2-induced apoptosis in MM cell...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2002-10-3146
更新日期：2003-05-01 00:00:00
abstract：:Hematopoiesis is tightly controlled by transcription regulatory networks, but how and when specific transcription factors control lineage commitment are still largely unknown. Within the hematopoietic stem cell (Lin(-)Sca-1(+)c-Kit(+)) compartment these lineage-specific transcription factors are expressed at low level...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-03-275404
更新日期：2010-11-18 00:00:00
abstract：:Chemotherapy-related myeloid neoplasia (t-MN) is a significant late toxicity concern after cancer therapy. In the randomized intergroup phase 3 E2997 trial, initial therapy of chronic lymphocytic leukemia with fludarabine plus cyclophosphamide (FC) compared with fludarabine alone yielded higher complete and overall re...
journal_title：Blood
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1182/blood-2011-03-342485
更新日期：2011-09-29 00:00:00
abstract：:To assess the frequency and significance of nonrandom abnormalities of chromosome 9p in childhood acute lymphoblastic leukemia (ALL), we analyzed our experience with 398 consecutive cases with completely banded karyotypes. Forty cases (10%) with abnormalities of 9p were identified: 26 with deletions, nine with unbalan...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
