Abstract:
BACKGROUND:Tartrate-resistant acid phosphatase (AcP) 5b is a marker of osteoclastic activity and bone resorption. Immunoassays for serum TRAcP may lack sensitivity and specificity because of the presence of non-bone isoform 5a. The purpose of this study was to isolate the serum isoforms, quantify their disease-related expressions, and test an improved immunoassay for TRAcP 5b. METHODS:We separated TRAcP isoforms chromatographically from pooled sera of healthy, rheumatoid arthritis (RA) and endstage renal disease (ESRD) subjects. TRAcP isoforms were identified by electrophoresis and quantified by biochemical and immunochemical assays. Serum TRAcP activity in healthy, RA, and ESRD cohorts was assessed at pH 5.5 and 6.1, and compared with bone alkaline phosphatase (BAP) and N-telopeptides of type I collagen (NTx). RESULTS:TRAcP isoforms 5a and 5b were present in all sera; 5b was identical to osteoclastic TRAcP. In serum from healthy subjects, 5a accounted for 87% of the enzyme protein but only 55% of the activity. In RA, both isoforms were increased two- to threefold in protein, but their specific activities were subnormal. In ESRD, only 5b was abnormal, being increased fivefold in protein and threefold in activity. In RA sera, TRAcP activity did not correlate with either BAP or NTx. In ESRD sera, TRAcP activity correlated with BAP and NTx only when measured at pH 6.1. CONCLUSIONS:All sera contained both TRAcP isoforms 5a and 5b, but only 5b was present in bone. TRAcP isoform expression was variable in different diseases. Measurement of TRAcP activity at pH 6.1 improves the specificity of immunoassay for isoform 5b.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Janckila AJ,Takahashi K,Sun SZ,Yam LTsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
74-80issue
1eissn
0009-9147issn
1530-8561journal_volume
47pub_type
杂志文章abstract::We have developed and evaluated a new procedure for detecting trends in quality-control measurements and applied it to laboratory data. The method requires the use of sequential or "moving" slope estimates to identify trends. Formulae are derived to estimate the regression error for the moving slope directly from the ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::As the clinical utility of glycated hemoglobin (gHb) measurement increases, so does the need for standardization of values between different methods and different laboratories. Using three different methods, we examined the feasibility of interlaboratory standardization of gHb measurement. A liquid-chromatographic (HP...
journal_title:Clinical chemistry
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doi:
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abstract::We can detect the beta-globin gene sickle cell mutation by using an assay based on the ligase chain reaction. The simultaneous amplification of the human growth hormone gene in the same reaction serves as a control for the amount of template DNA or amplification efficiency. Ligation products, which are biotinylated at...
journal_title:Clinical chemistry
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doi:
更新日期:1997-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-04-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1993-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.016311
更新日期:2004-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.083360
更新日期:2007-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1978-09-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1999-12-01 00:00:00
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journal_title:Clinical chemistry
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doi:10.1373/clinchem.2005.061267
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doi:
更新日期:1979-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
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