Polymerase chain reaction for the laboratory diagnosis of aseptic meningitis and encephalitis.

Abstract:

:A protocol for testing cerebrospinal fluid specimens using a range of PCR assays for the diagnosis of central nervous system infection was developed and used to test prospectively 383 specimens. PCR assays were used for the detection of adenovirus, Borrelia burgdorferi, enteroviruses, Epstein Barr virus, cytomegalovirus, herpes simplex virus, human herpes virus type 6, JC virus, Leptospira interrogans, Listeria monocytogenes, lymphocytic choriomeningitis virus, measles virus, mumps virus, Mycobacterium sp. , Mycoplasma pneumoniae, Toxoplasma gondii and varicella zoster virus. Of the 383 specimens tested in this study, 46 (12.0%) were found to be positive. The microorganisms detected were CMV, enterovirus, Epstein Barr virus, herpes simplex virus, human herpes virus type 6, JC virus, L. monocytogenes, Mycobacterium genus, Toxoplasma gondii and varicella zoster virus. The introduction of the PCR protocol described has improved the diagnosis of a range of central nervous system infections in our laboratory. We believe however that further evaluation of these assays in immunocompromised patients is necessary to better determine the predictive value of positive PCR results in these patient groups.

journal_name

Arq Neuropsiquiatr

authors

Chesky M,Scalco R,Failace L,Read S,Jobim LF

doi

10.1590/s0004-282x2000000500008

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

836-42

issue

3B

eissn

0004-282X

issn

1678-4227

pii

S0004-282X2000000500008

journal_volume

58

pub_type

杂志文章
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    abstract::The case of a 24 years-old woman with weakness since the teens and progressive loss of muscle strength is reported. The muscle biopsy showed increased number of mitochondria. In two occasions the respiratory chain enzymes showed important reduction of the succinate-cytochrome-C-reductase, suggesting a possible defect ...

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    doi:10.1590/s0004-282x1989000400013

    authors: Werneck LC,DiMauro S

    更新日期:1989-12-01 00:00:00

  • Aliocha Dostoevski's death during an epileptic seizure.

    abstract::Mortality due to epilepsy is of great concern worldwide. Individuals with epilepsy have a two- or three-fold risk of death when compared to the general population. Based on biographical data and Anna Grigoriévna Dostoevskaia's memories, the authors concluded that a prolonged episode of status epilepticus was the culpr...

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    doi:10.1590/0004-282X20160147

    authors: Amâncio EJ,Kowacs PA,Takeshita BT,Nascimento FA,Teive HA

    更新日期:2016-11-01 00:00:00

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    doi:10.1590/s0004-282x2004000500029

    authors: Honorato D,Borges W,Vargas AA,Ramina R

    更新日期:2004-09-01 00:00:00

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    abstract::Multiple sclerosis (MS) is an inflammatory, autoimmune, demyelinating, and degenerative central nervous system disease. Even though the etiology of MS has not yet been fully elucidated, there is evidence that genetic and environmental factors interact to cause the disease. Among the main environmental factors studied,...

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    pub_type: 共识发展会议,杂志文章

    doi:10.1590/0004-282X20130252

    authors: Brum DG,Comini-Frota ER,Vasconcelos CC,Dias-Tosta E

    更新日期:2014-02-01 00:00:00

  • [Endolymphatic sac adenocarcinoma: case report].

    abstract::A case of endolymphatic sac adenocarcinoma is reported and the literature is reviewed. The clinical picture was presented by vertigo and progressive hearing loss caused by a tumor of the endolymphatic sac. The surgical removal was complete, via a retro and translabyrinthine approach. Endolymphatic sac tumors are local...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章,评审

    doi:

    authors: Silveira RL,Gusmão SS,Pittella JE,Santos SP

    更新日期:2002-09-01 00:00:00

  • [Carpal tunnel syndrome: sensory median-radial latency difference versus conduction studies in 1059 hands (668 cases)].

    abstract::Between January 1989 and June 1996, 1,059 carpal tunnel syndrome hands (CTS) from 668 patients were studied. None had been previously operated and all had bilateral conduction studies; peripheral neuropathy was excluded. The patients were selected with sensory median/radial difference (MRD) > or = 1.0 ms that strongly...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x1999000200008

    authors: Kouyoumdjian JA

    更新日期:1999-06-01 00:00:00

  • What should you know about limbic encephalitis?

    abstract::Autoimmune encephalitis is an inflammatory disorder characterized by a subacute impairment of short-term memory, psychiatric features and seizures. It is often associated with a variety of other neurological symptoms, and its differential diagnosis is wide, leading to challenges in its recognition. It used to be regar...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章,评审

    doi:10.1590/s0004-282x2012001000012

    authors: Machado S,Pinto AN,Irani SR

    更新日期:2012-10-01 00:00:00

  • [Charcot-Marie-Tooth disease. Electromyographic studies in 45 patients].

    abstract::The electrophysiological studies of 45 patients with Charcot-Marie-Tooth disease (CMT) are presented. The nerve conduction of the motor median and ulnar nerves permitted us to separate our patients in two types: type I (demyelinating) with motor nerve conduction (MNC) below 38 m/s (11 cases) and type II with MNC norma...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 临床试验,杂志文章

    doi:10.1590/s0004-282x1995000400002

    authors: Freitas MR,Nascimento OJ,Nevares MT,Escada TM

    更新日期:1995-09-01 00:00:00

  • [Epidemiological study of the traumatic brachial plexus injuries in adults].

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    journal_title:Arquivos de neuro-psiquiatria

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    doi:10.1590/s0004-282x2006000100018

    authors: Flores LP

    更新日期:2006-03-01 00:00:00

  • Charcot and vascular Parkinsonism.

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    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 传,历史文章,杂志文章

    doi:10.1590/0004-282X20170007

    authors: Teive HA,Germiniani FM,Munhoz RP

    更新日期:2017-03-01 00:00:00

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    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x2004000400001

    authors: Arruda AP,Marques W Jr,Foss NT,Garbino JA,Virmond M,Barreira AA

    更新日期:2004-09-01 00:00:00

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    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/0004-282x20140165

    authors: Frigeri T,Rhoton A,Paglioli E,Azambuja N

    更新日期:2014-10-01 00:00:00

  • [Compliance in epilepsy. I. Concept factors and influence factors].

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    更新日期:1991-06-01 00:00:00

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    journal_title:Arquivos de neuro-psiquiatria

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    doi:10.1590/0004-282x20130028

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    更新日期:2013-05-01 00:00:00

  • [Clinical and laboratory aspects of acute bacterial meningitis in infants].

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    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:

    authors: Lucena R,Gomes I,Cardoso E,Goes J,Nunes L,Cardoso A,Rodrigues B,Souza M,Novaes MA,Melo A

    更新日期:2002-06-01 00:00:00

  • Non-epileptiform EEG abnormalities: an overview.

    abstract::More than 80 years after its introduction by Hans Berger, the electroencephalogram (EEG) remains as an important supplementary examination in the investigation of neurological disorders and gives valuable and accurate information about cerebral function. Abnormal EEG findings may include ictal patterns, interictal epi...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章,评审

    doi:10.1590/s0004-282x2011000600020

    authors: Andraus ME,Alves-Leon SV

    更新日期:2011-10-01 00:00:00

  • [Does semi-quantitative analysis of FDG-PET have any additional value in the diagnosis of mesial temporal sclerosis?].

    abstract:UNLABELLED:The aim of this study was to compare qualitative visual analysis with semi-quantitative analysis in the diagnosis of mesial temporal sclerosis (MTS) using FDG-PET. METHODS:This study included 21 patients with histopathological confirmation of MTS. FDG-PET visual analysis data were based on clinical reports ...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x2001000600007

    authors: Duarte PS,Zhuang H,Couturier O,Alavi A

    更新日期:2001-12-01 00:00:00

  • Computerized tomography findings in Fahr's syndrome.

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    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x2004000500009

    authors: Faria AV,Pereira IC,Nanni L

    更新日期:2004-09-01 00:00:00

  • Prevalence of multiple sclerosis in Goiânia, Goiás, Brazil.

    abstract:OBJECTIVE:Multiple sclerosis (MS) prevalence, in some cities in Brazil, was estimated and was found to range from 0.75 to 30.7/100,000. The reasons for such a large variation in rates of prevalence are not clear, but environment and genetics help to explain this phenomenon. METHODS:A cross-sectional study using three ...

    journal_title:Arquivos de neuro-psiquiatria

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  • Correlation of sleep macrostructure parameters and idiopathic epilepsies.

    abstract::Sleep and epilepsy share some common mechanisms. The objective of the present investigation was to study the macrostructure of sleep in patients with idiopathic epilepsies, focal and generalized, comparing these two groups to each other and to a control group of 12 individuals without epilepsy. A total of 35 polysomno...

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    pub_type: 杂志文章

    doi:10.1590/s0004-282x2002000300002

    authors: Barreto JR,Fernandes RM,Sakamoto AC

    更新日期:2002-06-01 00:00:00

  • [Hyperexcitability syndrome in the neonatal period: study of etiological factors].

    abstract::Forty-five newborn infants with hyperexcitability lasting more than 72 hours were studied in the neonatal period. The association with metabolic disturbances, mainly hypocalcemia, was observed in 53% of cases. Neonatal asphyxia was observed in 21 cases (46%), although all infants had 5 minutes Apgar scores above 6. On...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x1993000100008

    authors: Gherpelli JL,Casagrande MS,Kfuri JM,Costa HP

    更新日期:1993-03-01 00:00:00

  • Safety of transcranial direct current stimulation in a patient with deep brain stimulation electrodes.

    abstract:BACKGROUND:Transcranial direct current stimulation (tDCS) has been investigated in movement disorders, making it a therapeutic alternative in clinical settings. However, there is still no consensus on the most appropriate treatment protocols in most cases, and the presence of deep brain stimulation (DBS) electrodes has...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

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    authors: Iannone A,Allam N,Brasil-Neto JP

    更新日期:2019-03-01 00:00:00

  • Comparison between digital subtraction angiography and magnetic resonance angiography in investigation of nonlacunar ischemic stroke in young patients: preliminary results.

    abstract:PURPOSE:We preliminarily investigated the relevance of performing digital subtraction angiography (DSA) in addition to magnetic resonance angiography (MRA) in definition of ischemic stroke etiology in young patients. METHOD:DSAs and MRAs from 17 young patients with nonlacunar ischemic stroke were blindly analyzed and ...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x2006000300001

    authors: Conforto AB,Fregni F,Puglia P Jr,Leite Cda C,Yamamoto FI,Coracini KF,Scaff M

    更新日期:2006-06-01 00:00:00

  • Post-poliomyelitis syndrome: case report.

    abstract::The post-polio syndrome (PPS) is an entity characterized for an episode of muscular weakness and/or abnormal muscular fatigue in individuals that had presented acute polio years before. We report the case of PPS in a patient, 40 years, that thirty-five years after had had paralytic poliomyelitis, developed new symptom...

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    pub_type: 杂志文章

    doi:10.1590/s0004-282x2007000300033

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    更新日期:2007-06-01 00:00:00

  • [Expansive laminoplasty: an alternative for the treatment of cervical spondyloarthrosis].

    abstract::We describe the surgical technique of expansive cervical laminoplasty and analyse the results in 28 patients treated by this method for cervical spondylotic myelopathy with a minimum follow-up of six months. Twenty-four patients (86%) had clinical improvement according to the Nurick scale while three (10%) had no impr...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x2005000600018

    authors: de Andrade GC,da Silveira RL,Arantes AA Jr,Pinheiro N Jr,Rocha EM

    更新日期:2005-12-01 00:00:00

  • [Carotid-basilar anastomosis and persistence of the primitive hypoglossal artery. Report of a case].

    abstract::A case of carotid-basilar anastomose with persistence of the primitive hypoglossal artery is reported. The authors related aspects of intracranial aneurysm. Anatomical, embryological, physiopathological and angiographical aspects are discussed. ...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 杂志文章

    doi:10.1590/s0004-282x1980000300011

    authors: Bueno MG,Riff PE,Nassar KE,Aisen J

    更新日期:1980-09-01 00:00:00

  • [Language impairment in the early stages of Alzheimer's disease].

    abstract::Alzheimer disease (AD) is usually associated with cognitive, language and behavioral impairments, which can get more and more serious as the disease progresses. The aim of this study is to verify language disorders in the early stages of this disease. Twelve patients meeting criteria for problable AD were evaluated by...

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    pub_type: 杂志文章

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    authors: Ortiz KZ,Bertolucci PH

    更新日期:2005-06-01 00:00:00

  • [West syndrome: clinical and electroencephalographic follow up of 70 patients and response to its treatment with adrenocorticotropic hormone, prednisone, vigabatrin, nitrazepam and valproate].

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    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 临床试验,杂志文章

    doi:10.1590/s0004-282x2000000400014

    authors: Antoniuk SA,Bruck I,Spessatto A,Halick SM,de Bruyn LR,Meister E,de Paola D

    更新日期:2000-09-01 00:00:00

  • Dementia pugilistica with clinical features of Alzheimer's disease.

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    pub_type: 杂志文章

    doi:10.1590/s0004-282x2007000500019

    authors: Areza-Fegyveres R,Rosemberg S,Castro RM,Porto CS,Bahia VS,Caramelli P,Nitrini R

    更新日期:2007-09-01 00:00:00

  • [Attention deficit disorder: treatment with methylphenidate].

    abstract::The authors present the results of the treatment with methylphenidate in 24 children with attention deficit disorder based on DSM-III. The prescribed dose was 5 to 10 mg per day, once or twice a day. The results were good in 79.1% of the patients and poor in 16.6%. The drug was withdrawn in one patient because of side...

    journal_title:Arquivos de neuro-psiquiatria

    pub_type: 临床试验,杂志文章

    doi:10.1590/s0004-282x1996000100004

    authors: Guerreiro MM,Montenegro MA,Piva RT,Moura-Ribeiro MV

    更新日期:1996-03-01 00:00:00