Abstract:
:Dendritic cells (DCs) represent a family of ontogenically distinct leukocytes involved in immune response regulation. The ability of DCs to stimulate T-cell immunity has led to their use as vectors for immunotherapy vaccines. However, it is unclear whether and to what degree in vitro-generated DCs are representative of DCs that develop in vivo. Treatment of mice with human Flt3 ligand (FL) dramatically increases the number of DCs. We report here that administration of FL to healthy human volunteers increased the number of circulating CD11c(+ )IL-3Ralpha(low) DC (mean 44-fold) and CD11c(-) IL-3Ralpha(high) DC precursors (mean 12-fold). Moreover, the CD11c(+ )DCs were efficient stimulators of T cells in vitro. Thus, FL can expand the number of circulating, functionally competent human DCs in vivo.
journal_name
Bloodjournal_title
Bloodauthors
Maraskovsky E,Daro E,Roux E,Teepe M,Maliszewski CR,Hoek J,Caron D,Lebsack ME,McKenna HJsubject
Has Abstractpub_date
2000-08-01 00:00:00pages
878-84issue
3eissn
0006-4971issn
1528-0020journal_volume
96pub_type
杂志文章相关文献
BLOOD文献大全abstract::The purpose of this report was to evaluate scintigraphy analysis of Southern blot hybridization as a method to quantify the breakpoint cluster region (BCR) rearrangement of Philadelphia chromosome (Ph)+ chronic myelogenous leukemia (CML). Cytogenetic and molecular studies performed simultaneously on 474 bone marrow an...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-05-15 00:00:00
abstract::The improved outcome of acquired aplastic anemia (AA) has revealed later complications, such as myelodysplastic syndrome (MDS) and acute myelogenous leukemia (AML). We retrospectively analyzed 167 children with severe acquired AA. Eleven of 50 children treated with cyclosporin (CSA) and recombinant human granulocyte c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Cell cycle checkpoints ensure orderly progression of events during cell division. A microtubule damage (MTD)-induced checkpoint has been described in G(1) phase of the cell cycle (G(1)MTC) for which little is known. The present study shows that the G(1)MTC is intact in activated T lymphocytes from mice with the p21(wa...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.5.1505
更新日期:2001-03-01 00:00:00
abstract::Prophylactic treatment in severe hemophilia is very effective but is limited by cost issues. The implementation of 2 different prophylactic regimens in The Netherlands and Sweden since the 1970s may be considered a natural experiment. We compared the costs and outcomes of Dutch intermediate- and Swedish high-dose prop...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-12-470898
更新日期:2013-08-15 00:00:00
abstract::Beginning in 1985, patients in British Columbia with Hodgkin lymphoma (HL) that was not controlled by conventional chemotherapy routinely underwent high-dose chemotherapy and autologous stem cell transplantation (HD-ASCT). Long-term complications of HD-ASCT have become apparent as more patients survive without recurre...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-12-4689
更新日期:2005-08-15 00:00:00
abstract::Immune thrombocytopenia (ITP) is a bleeding disorder in which antibodies and/or T cells lead to enhanced peripheral platelet destruction and reduced bone marrow platelet production. Several reports have observed that ITP is associated with a peripheral deficiency of tolerance-inducing CD4+CD25+FoxP3+ T regulatory cell...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-02-413526
更新日期:2012-09-06 00:00:00
abstract::Analysis of a growing number of chromosomal translocations in human tumors have shown that they frequently result in gene fusions encoding chimeric proteins. We have characterized the recurrent t(12;21)(p12;q22) translocation present in human B-lineage acute leukemias. This translocation fused two genes, tel and AML1,...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-06-15 00:00:00
abstract::Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in pla...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract::Red blood cell (RBC) transfusion is unique as a common large-scale intravenous introduction of foreign tissue and provides a valuable opportunity to study human immunologic response to intravenous foreign antigen. Patients receiving RBC transfusions are at risk of forming alloantibodies against donor RBC antigens, and...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-03-146415
更新日期:2008-09-15 00:00:00
abstract::T-cell development requires a series of discrete selection and activation signals delivered to maturing progenitors in the thymic cortex and medulla. We have previously shown the constitutive activity of the integrin, alpha4beta1 (VLA4), on a unique subpopulation of immature cortical thymocytes and proposed a role for...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::The Leukemia and Lymphoma Molecular Profiling Project recently published results from DNA microarray analyses of 240 diffuse large B-cell lymphomas (DLBCLs). Four gene expression "signatures" were identified as correlated with patient outcome, including the major histocompatibility complex (MHC) class II genes (eg, HL...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-07-2365
更新日期:2004-06-01 00:00:00
abstract::We report an RNA targeting strategy, which selectively degrades bcr/abl mRNA in chronic myelogenous leukemia (CML) cells. A 2', 5'-tetraadenylate activator (2-5A) of RNase L was chemically linked to oligonucleotide antisense directed against either the fusion site or against the translation start sequence in bcr/abl m...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Hemoglobin and ferritin iron content have been followed during differentiation in tissue cultures of murine erythroleukemia cells (MELC) using the techniques of Mössbauer spectroscopy and electron microscopy. In undifferentiated cells grown without DMSO, only iron stored in ferritin was detected. The amount of iron in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Ischemia/reperfusion (I/R) injury in the kidney is a major cause of acute kidney injury (AKI) in humans and is associated with significantly high mortality. To identify genes that modulate kidney injury and repair, we conducted genome-wide expression analysis in the rat kidneys after I/R and found that the mRNA levels...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-02-338061
更新日期:2011-08-18 00:00:00
abstract::Overexpression of transcription factors runt-related transcription factor 1 (RUNX1) and nuclear factor, erythroid-derived 2 (NF-E2) was reported in granulocytes of patients with polycythemia vera and other myeloproliferative neoplasms (MPNs). Further, a transgenic mouse overexpressing the NF-E2 transgene was reported ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-10-534222
更新日期:2014-01-16 00:00:00
abstract::Peripheral lymphocytes from normal individuals and from patients with chronic lymphocytic leukemia (CLL) were cultured in vitro for 1-7 days. The growth response to phytohemagglutinin (PHA) was quantitated by the incorporation of tritiated uridine into RNA nucleotide during a 2-hr pulse with the radioisotope. While th...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::Isocitrate dehydrogenase 1 mutation (IDH1-R132H) was recently identified in acute myeloid leukemia with normal cytogenetics. The mutant enzyme is thought to convert α-ketoglutarate to the pathogenic 2-hydroxyglutarate (2-HG) that affects DNA methylation via inhibition of ten-eleven translocation 2. However, the role o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-09-601187
更新日期:2015-05-07 00:00:00
abstract::Acquired thrombotic thrombocytopenic purpura (TTP) is a life-threatening disease characterized by thrombotic microangiopathy leading to end-organ damage. The standard of care (SOC) treatment is therapeutic plasma exchange (TPE) alongside immunomodulation with steroids, with increasing use of rituximab +/- other immuno...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020006052
更新日期:2020-12-06 00:00:00
abstract::Nijmegen breakage syndrome 1 (NBS1) is a component of the MRE11 complex, which is a sensor of DNA double-strand breaks and plays a crucial role in the DNA damage response. Because activated macrophages produce large amounts of reactive oxygen species (ROS) that can cause DNA lesions, we examined the role of NBS1 in ma...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-04-637371
更新日期:2015-11-26 00:00:00
abstract::Maintaining a steady pool of self-renewing hematopoietic stem cells (HSCs) is critical for sustained production of multiple blood lineages. Many transcription factors and molecules involved in chromatin and epigenetic modifications have been found to be critical for HSC self-renewal and differentiation; however, their...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-09-306563
更新日期:2011-02-17 00:00:00
abstract::Platelets contain and release several matrix metalloproteinases (MMPs). Among these, active MMP-2 enhances platelet aggregation by favoring the activation of phosphatidylinositol 3- kinase (PI3K) and contributes to arterial thrombosis. The platelet surface target of MMP-2 and the mechanism through which it primes plat...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-06-724245
更新日期:2017-02-16 00:00:00
abstract::Peripheral T-cell lymphomas (PTCLs) are aggressive tumors in which the current therapy based on multiagent chemotherapy is not successful. Since cytochrome P450 3A subfamily (CYP3A) enzymes are involved in the inactivation of chemotherapy drugs, we hypothesized that CYP3A and P-glycoprotein (MDR1) expression in these ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-02-075036
更新日期:2007-11-01 00:00:00
abstract::Multiple myeloma is characterized by genomic alterations frequently involving gains and losses of chromosomes. Single nucleotide polymorphism (SNP)-based mapping arrays allow the identification of copy number changes at the sub-megabase level and the identification of loss of heterozygosity (LOH) due to monosomy and u...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-02-005496
更新日期:2006-09-01 00:00:00
abstract::Enzymes of the glycolytic pathway as well as some ancillary enzymes were studied in normal red cells parasitized with Plasmodium falciparum in culture at varying parasitemias as well as in isolated parasites. The levels of all enzymes except diphosphoglycerate mutase, glucose-6-phosphate dehydrogenase, and adenylate k...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::IgM-secreting plasma cell tumors are rare variants of typical isotype-switched multiple myeloma with a similar disease outcome. To probe the origin and clonal history of these tumors, we have analyzed V(H) gene sequences in 6 cases. Potentially functional tumor-derived V(H) genes were all derived from V(H)3, with the ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Fibrin forms the cohesive network of hemostatic plugs and thrombi, and it also provides the temporary matrix for initial support of healing and revascularization. Because cell proliferation is needed for revascularization after vessel injury, we have characterized structural requirements of fibrin needed to support ce...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The transcription factor (TF) RUNX1 cooperates with lineage-specifying TFs (eg, PU.1/SPI1) to activate myeloid differentiation genes, such as macrophage and granulocyte macrophage colony-stimulating factor receptors (MCSFR and GMCSFR). Disruption of cooperative gene activation could contribute to aberrant repression o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-10-312512
更新日期:2011-06-16 00:00:00
abstract::Ibrutinib is highly efficacious and used at 420 mg/d for treatment of chronic lymphocytic leukemia (CLL). We previously demonstrated a decline in Bruton's tyrosine kinase (BTK) protein levels in CLL cells after 1 cycle of ibrutinib, suggesting ibrutinib dose could be lowered after the first cycle without loss of biolo...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2018-06-860593
更新日期:2018-11-22 00:00:00
abstract::Myeloma immunosurveillance remains incompletely understood. We have demonstrated proteolytic processing of the matrix proteoglycan, versican (VCAN), in myeloma tumors. Whereas intact VCAN exerts tolerogenic activities through Toll-like receptor 2 (TLR2) binding, the immunoregulatory consequences of VCAN proteolysis re...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-03-705780
更新日期:2016-08-04 00:00:00
abstract::The costimulatory effects of CD27 on T lymphocyte effector function and memory formation has been confined to evaluations in mouse models, in vitro human cell culture systems, and clinical observations. Here, we tested whether CD27 costimulation actively enhances human T-cell function, expansion, and survival in vitro...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-03-344275
更新日期:2012-01-19 00:00:00