The calcimimetic R-568 induces apoptotic cell death in prostate cancer cells.

Abstract:

BACKGROUND:Increased serum level of parathyroid hormone (PTH) was found in metastatic prostate cancers. Calcimimetic R-568 was reported to reduce PTH expression, to suppress cell proliferation and to induce apoptosis in parathyroid cells. In this study, we investigated the effect of R-568 on cellular survival of prostate cancer cells. METHODS:Prostate cancer cell lines LNCaP and PC-3 were used in this study. Cellular survival was determined with MTT, trypan blue exclusion and fluorescent Live/Death assays. Western blot assay was utilized to assess apoptotic events induced by R-568 treatment. JC-1 staining was used to evaluate mitochondrial membrane potential. RESULTS:In cultured prostate cancer LNCaP and PC-3 cells, R-568 treatment significantly reduced cellular survival in a dose- and time-dependent manner. R-568-induced cell death was an apoptotic event, as evidenced by caspase-3 processing and PARP cleavage, as well as JC-1 color change in mitochondria. Knocking down calcium sensing receptor (CaSR) significantly reduced R-568-induced cytotoxicity. Enforced expression of Bcl-xL gene abolished R-568-induced cell death, while loss of Bcl-xL expression led to increased cell death in R-568-treated LNCaP cells,. CONCLUSION:Taken together, our data demonstrated that calcimimetic R-568 triggers an intrinsic mitochondria-related apoptotic pathway, which is dependent on the CaSR and is modulated by Bcl-xL anti-apoptotic pathway.

journal_name

J Exp Clin Cancer Res

authors

Li H,Ruan G,Li Z,Liu Z,Zheng X,Zheng H,Cheng G,Li B,Zhan M

doi

10.1186/1756-9966-28-100

subject

Has Abstract

pub_date

2009-07-14 00:00:00

pages

100

eissn

0392-9078

issn

1756-9966

pii

1756-9966-28-100

journal_volume

28

pub_type

杂志文章
  • Transfection of mammalian cells by the methods of receptor mediated gene transfer and particle bombardment.

    abstract::The efficacy of currently developed methods for gene transfer into mammalian cells depends primarily on the transfection technique, and also on the type of targeted cells. Considering the importance of gene transfer in the creation of gene therapies, our study was aimed at the assessment of transfection capacity of re...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Novaković S,Knezević M,Golouh R,Jezersek B

    更新日期:1999-12-01 00:00:00

  • Cdk1/P34Cdc2 and P21waf expression in colorectal adenomas and carcinomas.

    abstract::p21WAF1,CIP1,SDI 1 is a specific inhibitor of cyclin-dependent kinase and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Cdk1/p34cdc2 plays a crucial role during mitosis. The immunoreactivity of p21 WAF1,CIP1,SDI 1 and cdk1/p34cdc2 was evaluated in adenomas and adenocarcinomas of the hum...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Egilmez R,Elagöz S,Kanik EA

    更新日期:2001-12-01 00:00:00

  • A dosimetric comparison of different treatment plans of palliative spinal bone irradiation: analysis of dose coverage with respect to ICRU 50 Report.

    abstract:BACKGROUND:This study aimed to analyze three-dimensional (3D) dosimetric data of conventional two-dimensional (2D) palliative spinal bone irradiation using different reference points and treatment plans with respect to the International Commission on Radiation Units and Measurements (ICRU) Report 50. METHODS:Forty-fiv...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-28-2

    authors: Andic F,Baz Cifci S,Ors Y,Niang U,Dirier A,Adli M

    更新日期:2009-01-07 00:00:00

  • Clinically meaningful response to the EGFR tyrosine kinase inhibitor gefitinib ('Iressa', ZD1839) in non small cell lung cancer.

    abstract::Preclinical research and ongoing clinical trials are validating epidermal growth factor receptor (EGFR) as a suitable molecular target in cancer therapy. Here we report the case of a 39-year-old non-smoking man with heavily pretreated stage IV bronchioloalveolar NSCLC who was treated with gefitinib ('Iressa', ZD1839) ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Gelibter A,Ceribelli A,Milella M,Mottolese M,Vocaturo A,Cognetti F

    更新日期:2003-09-01 00:00:00

  • Correction to: Liquid biopsy and tumor heterogeneity in metastatic solid tumors: the potentiality of blood samples.

    abstract::An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,已发布勘误

    doi:10.1186/s13046-020-01615-w

    authors: Russano M,Napolitano A,Ribelli G,Iuliani M,Simonetti S,Citarella F,Pantano F,Dell'Aquila E,Anesi C,Silvestris N,Argentiero A,Solimando AG,Vincenzi B,Tonini G,Santini D

    更新日期:2020-06-24 00:00:00

  • JunB promotes cell invasion, migration and distant metastasis of head and neck squamous cell carcinoma.

    abstract:BACKGROUND:While treatment failure in cases of head and neck squamous cell carcinoma (HNSCC) frequently takes the form of locoregional recurrences and distant metastasis, our understanding of the mechanisms of metastasis in HNSCC is limited. We initially performed the upstream and key nodes analysis together with whole...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0284-4

    authors: Hyakusoku H,Sano D,Takahashi H,Hatano T,Isono Y,Shimada S,Ito Y,Myers JN,Oridate N

    更新日期:2016-01-12 00:00:00

  • Toxicity and morbility after isolated lower limb perfusion in 242 chemo-hyperthermal treatments for cutaneous melanoma: the experience of the Tuscan Reference Centre.

    abstract:BACKGROUND:The aim of this retrospective study was to assess the results concerning the regional and systemic toxicity and complications in 242 chemo-hyperthermal treatments (HILPs) for lower limb melanoma. PATIENTS AND METHODS:60 HILPs (G-A) were performed with mild HT plus L-PAM (10 mg/lt) +/- D-actimomycin; 74 HILP...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-27-67

    authors: Pace M,Gattai R,Matteini M,Mascitelli EM,Bechi P

    更新日期:2008-11-12 00:00:00

  • MiR-302a/b/c/d cooperatively sensitizes breast cancer cells to adriamycin via suppressing P-glycoprotein(P-gp) by targeting MAP/ERK kinase kinase 1 (MEKK1).

    abstract:BACKGROUND:The importance of individual microRNAs (miRNAs) in tumor has been established in different cancers. However, their association with tumor chemoresistance has not been fully understood. Previously, we found two novel MDR-associated microRNAs (miRNAs). In this report, we investigated the combined effects of mi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0300-8

    authors: Zhao L,Wang Y,Jiang L,He M,Bai X,Yu L,Wei M

    更新日期:2016-02-03 00:00:00

  • Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice.

    abstract:BACKGROUND:Metastasis via the lymphatic system is promoted by lymphangiogenesis. Alterations of the lymphatic channels during the progression of metastasis to regional lymph nodes (LNs) remain unexplored. To examine whether tumor-induced LN lymphangiogenesis controls metastasis to regional LNs, we investigated cervical...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-31-83

    authors: Ozasa R,Ohno J,Iwahashi T,Taniguchi K

    更新日期:2012-10-02 00:00:00

  • Adjuvant chemotherapy for resected non-small-cell lung cancer: future perspectives for clinical research.

    abstract::Adjuvant chemotherapy for non-small-cell lung carcinoma (NSCLC) is a debated issue in clinical oncology. Although it is considered a standard for resected stage II-IIIA patients according to the available guidelines, many questions are still open. Among them, it should be acknowledged that the treatment for stage IB d...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-30-115

    authors: Bonomi M,Pilotto S,Milella M,Massari F,Cingarlini S,Brunelli M,Chilosi M,Tortora G,Bria E

    更新日期:2011-12-29 00:00:00

  • Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT.

    abstract:BACKGROUND:Antibody Directed Enzyme Prodrug Therapy (ADEPT) can be used to generate cytotoxic agents at the tumor site. To date non-human enzymes have mainly been utilized in ADEPT. However, these non-human enzymes are immunogenic limiting the number of times that ADEPT can be administered. To overcome the problem of i...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-28-147

    authors: Afshar S,Olafsen T,Wu AM,Morrison SL

    更新日期:2009-12-03 00:00:00

  • Flow cytometric analysis of CK19 expression in the peripheral blood of breast carcinoma patients: relevance for circulating tumor cell detection.

    abstract:BACKGROUND:Immunocytochemistry and RT-PCR have been widely used for the detection of circulating tumor cells in patients with breast cancer but their specificity is limited. Our purpose is to utilize a convenient and specific technology to detect circulating tumor cells in breast cancer patients. METHODS:To determine ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-28-57

    authors: Wang L,Wang Y,Liu Y,Cheng M,Wu X,Wei H

    更新日期:2009-04-28 00:00:00

  • Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases.

    abstract:BACKGROUND:Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-014-0119-0

    authors: Cordiali-Fei P,Trento E,Giovanetti M,Lo Presti A,Latini A,Giuliani M,D'Agosto G,Bordignon V,Cella E,Farchi F,Ferraro C,Lesnoni La Parola I,Cota C,Sperduti I,Vento A,Cristaudo A,Ciccozzi M,Ensoli F

    更新日期:2015-01-16 00:00:00

  • Correlation effect of EGFR and CXCR4 and CCR7 chemokine receptors in predicting breast cancer metastasis and prognosis.

    abstract:BACKGROUND:The chemokine receptors CXCR4 and CCR7 play an important role in cancer invasion and metastasis. This study investigated the expression of CXCR4, CCR7, CXCL12, CCL21, and EGFR to illustrate the role of these biomarkers in breast cancer metastasis and prognosis. METHODS:The CXCR4, CCR7, CXCL12, CCL21, and EG...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-29-16

    authors: Liu Y,Ji R,Li J,Gu Q,Zhao X,Sun T,Wang J,Li J,Du Q,Sun B

    更新日期:2010-02-24 00:00:00

  • Palliative treatment of painful bone metastases from non-Hodgkin lymphoma with disodium pamidronate.

    abstract::We report a case of a 72-year-old male, with a known history of non-Hodgkin lymphoma of the left tonsil for two years, histologically proved and successfully treated by radical surgical excision in combination with external radiotherapy. He presented with diffuse bone pain the last month, especially at the lower left ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Pistevou-Gombaki K,Eleftheriadis N,Sofroniadis I,Makris P,Kouloulias V

    更新日期:2002-09-01 00:00:00

  • Prohibitin overexpression predicts poor prognosis and promotes cell proliferation and invasion through ERK pathway activation in gallbladder cancer.

    abstract:BACKGROUND:Prohibitin (PHB), a pleiotropic protein overexpressed in several tumor types, has been implicated in the regulation of cell proliferation, invasive migration and survival. However, PHB expression and its biological function in gallbladder cancer (GBC) remain largely unknown. METHODS:PHB and p-ERK protein ex...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0346-7

    authors: Cao Y,Liang H,Zhang F,Luan Z,Zhao S,Wang XA,Liu S,Bao R,Shu Y,Ma Q,Zhu J,Liu Y

    更新日期:2016-04-16 00:00:00

  • Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway.

    abstract:BACKGROUND:The cisplatin-resistance is still a main course for chemotherapy failure of lung cancer patients. Cisplatin-resistant cancer cells own higher malignance and exhibited increased metastatic ability, but the mechanism is not clear. In this study, we investigated the effects of Ataxia Telangiectasia Mutated (ATM...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1161-8

    authors: Shen M,Xu Z,Xu W,Jiang K,Zhang F,Ding Q,Xu Z,Chen Y

    更新日期:2019-04-08 00:00:00

  • A pilot study on the impact of known drug-drug interactions in cancer patients.

    abstract:BACKGROUND:When a patient concomitantly uses two or more drugs, a drug-drug interaction (DDI) can possibly occur, potentially leading to an increased or decreased clinical effect of a given treatment. Cancer patients are at high risk of such interactions because they commonly receive multiple medications. Moreover, mos...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-015-0201-2

    authors: Ussai S,Petelin R,Giordano A,Malinconico M,Cirillo D,Pentimalli F

    更新日期:2015-08-25 00:00:00

  • CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway.

    abstract:OBJECTIVE:To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro. METHODS:The expressions of FasL in HepG2 ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-48

    authors: Zheng J,Fu R,Li J,Wang X

    更新日期:2011-05-03 00:00:00

  • Medical decision making for melanoma of the glans penis.

    abstract::This case report describes a rare presentation of penile melanoma in which 3 successive primaries arose and were operated from an area of melanosis on the glans penis and prepuce. One of the major factors accounting for the poor prognosis of this patient was the long delay in presentation. This was largely due to the ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Testori A,Mazzarol G,Viale G,De Cobelli O,Lotti M,Rocco F,Andreoni B

    更新日期:1999-06-01 00:00:00

  • Co-administration of 20(S)-protopanaxatriol (g-PPT) and EGFR-TKI overcomes EGFR-TKI resistance by decreasing SCD1 induced lipid accumulation in non-small cell lung cancer.

    abstract:BACKGROUND:Non-small cell lung cancer (NSCLC) patients with sensitive epidermal growth factor receptor (EGFR) mutations are successfully treated with EGFR tyrosine kinase inhibitors (EGFR-TKIs); however, resistance to treatment inevitably occurs. Given lipid metabolic reprogramming is widely known as a hallmark of canc...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1120-4

    authors: Huang Q,Wang Q,Li D,Wei X,Jia Y,Zhang Z,Ai B,Cao X,Guo T,Liao Y

    更新日期:2019-03-15 00:00:00

  • Transcriptional and post-transcriptional controls of survivin in cancer cells: novel approaches for cancer treatment.

    abstract::Survivin is a novel member of the inhibitor of apoptosis (IAP) protein family, and its aberrant expression in cancer cells has been shown to be associated with tumorigenesis, cancer progression, radiation/drug resistance and shorter patient survival. Survivin is also expressed in certain human adult tissues and cells,...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Zhang M,Yang J,Li F

    更新日期:2006-09-01 00:00:00

  • Anti-tumour effects of all-trans retinoid acid on serous ovarian cancer.

    abstract:BACKGROUND:Annexin A2 is increased in serous ovarian cancer and plays an essential role in ovarian cancer invasion and metastasis. In combination with S100A10, annexin A2 plays an important role in the plasminogen activator system regulating plasmin production. The aim of this study was to investigate the potential uti...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-018-1017-7

    authors: Lokman NA,Ho R,Gunasegaran K,Bonner WM,Oehler MK,Ricciardelli C

    更新日期:2019-01-08 00:00:00

  • Low-molecular-weight heparins are superior to vitamin K antagonists for the long term treatment of venous thromboembolism in patients with cancer: a cochrane systematic review.

    abstract:BACKGROUND:Cancer and its therapies increase the risk of venous thromboembolism. Compared to patients without cancer, patients with cancer anticoagulated for venous thromboembolism are more likely to develop recurrent thrombotic events and major bleeding. Addressing all important outcomes including harm is of great imp...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-27-21

    authors: Akl EA,Barba M,Rohilla S,Terrenato I,Sperati F,Muti P,Schünemann HJ

    更新日期:2008-07-18 00:00:00

  • SLC29A1 single nucleotide polymorphisms as independent prognostic predictors for survival of patients with acute myeloid leukemia: an in vitro study.

    abstract:BACKGROUND:The mechanism behind poor survival of acute myeloid leukemia (AML) patients with 1-barabinofuranosylcytosine (Ara-C) based treatment remains unclear. This study aimed to assess the pharmacogenomic effects of Ara-C metabolic pathway in patients with AML. METHODS:The genotypes of 19 single nucleotide polymorp...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s13046-014-0090-9

    authors: Wan H,Zhu J,Chen F,Xiao F,Huang H,Han X,Zhong L,Zhong H,Xu L,Ni B,Zhong J

    更新日期:2014-11-15 00:00:00

  • Correction to: Targeting hypoxia in tumor: a new promising therapeutic strategy.

    abstract::In the original publication of this manuscript [1], Fig. 1 contained a typographical error ('Metabolic' incorrectly written as 'Metabolig'). ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 已发布勘误

    doi:10.1186/s13046-020-1532-1

    authors: Bosco MC,Orazi G,Del Bufalo D

    更新日期:2020-02-24 00:00:00

  • Viruses in human oral cancers.

    abstract::The role of viruses in the etiology of human oral cancer is critically reviewed. Available evidences show a positive correlation for human oral cancer with human papilloma virus (HPV), herpes simplex virus (HSV) and human herpes virus type-6 (HHV-6), having strongest association with HPV. These viruses may act alone o...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Vasudevan DM,Vijayakumar T

    更新日期:1998-03-01 00:00:00

  • GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment.

    abstract:BACKGROUND:The aim of this study was to investigate the effect of downregulating Hedgehog pathway by GANT61 on human glioma cells, examine the consequent changes of temozolomide (TMZ)-induced effects and explore the molecular mechanisms. METHODS:The cytotoxicity of a Gli1/2 inhibitor, GANT61 was examined both alone an...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0463-3

    authors: Li J,Cai J,Zhao S,Yao K,Sun Y,Li Y,Chen L,Li R,Zhai X,Zhang J,Jiang C

    更新日期:2016-11-28 00:00:00

  • MiR-200c sensitizes Olaparib-resistant ovarian cancer cells by targeting Neuropilin 1.

    abstract:BACKGROUND:Ovarian cancer (OC) is the most lethal gynecological malignancy and the second leading cause of cancer-related death in women. Treatment with PARP inhibitors (PARPi), such as Olaparib, has been recently introduced for OC patients, but resistance may occur and underlying mechanisms are still poorly understood...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1490-7

    authors: Vescarelli E,Gerini G,Megiorni F,Anastasiadou E,Pontecorvi P,Solito L,De Vitis C,Camero S,Marchetti C,Mancini R,Benedetti Panici P,Dominici C,Romano F,Angeloni A,Marchese C,Ceccarelli S

    更新日期:2020-01-02 00:00:00

  • Challenges and potential of PD-1/PD-L1 checkpoint blockade immunotherapy for glioblastoma.

    abstract::PD-1/PD-L1 checkpoint blockades have achieved significant progress in several kinds of tumours. Pembrolizumab, which targets PD-1, has been approved as a first-line treatment for advanced non-small cell lung cancer (NSCLC) patients with positive PD-L1 expression. However, PD-1/PD-L1 checkpoint blockades have not achie...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-019-1085-3

    authors: Wang X,Guo G,Guan H,Yu Y,Lu J,Yu J

    更新日期:2019-02-18 00:00:00