The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line.

Abstract:

:The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.

journal_name

J Exp Clin Cancer Res

authors

Ristic-Fira A,Nikolic D,Petrovic I,Ruzdijic S,Raffaele L,Sabini MG,Cirrone GA,Cuttone G,Farruggia G,Masotti L,Kanazir DT

keywords:

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

135-43

issue

1

eissn

0392-9078

issn

1756-9966

journal_volume

20

pub_type

杂志文章
  • Mitophagy promotes sorafenib resistance through hypoxia-inducible ATAD3A dependent Axis.

    abstract:BACKGROUND:The identification of novel targets for recovering sorafenib resistance is pivotal for Hepatocellular carcinoma (HCC) patients. Mitophagy is the programmed degradation of mitochondria, and is likely involved in drug resistance of cancer cells. Here, we identified hyperactivated mitophagy is essential for sor...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01768-8

    authors: Wu H,Wang T,Liu Y,Li X,Xu S,Wu C,Zou H,Cao M,Jin G,Lang J,Wang B,Liu B,Luo X,Xu C

    更新日期:2020-12-07 00:00:00

  • Efficacy and adverse effects of transdermal fentanyl and sustained-release oral morphine in treating moderate-severe cancer pain in Chinese population: a systematic review and meta-analysis.

    abstract:BACKGROUND:Previous meta-analysis suggested that transdermal fentanyl was not inferior to sustained-release oral morphine in treating moderate-severe cancer pain with less adverse effects. Now, we updated the data and performed a systematic review. METHODS:Updated cohort studies on transdermal fentanyl and oral morphi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,meta分析

    doi:10.1186/1756-9966-29-67

    authors: Yang Q,Xie DR,Jiang ZM,Ma W,Zhang YD,Bi ZF,Chen DL

    更新日期:2010-06-09 00:00:00

  • Expression and aberrant promoter methylation of Wnt inhibitory factor-1 in human astrocytomas.

    abstract:BACKGROUND:Wnt inhibitory factor-1(WIF-1) acts as a Wnt-antagonists and tumor suppressor, but hypermethylation of WIF-1 gene promoter and low expression activate Wnt signaling aberrantly and induce the development of various human tumors. With this work we intended to investigate the expression and promoter methylation...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-29-26

    authors: Yang Z,Wang Y,Fang J,Chen F,Liu J,Wu J,Wang Y

    更新日期:2010-03-24 00:00:00

  • Endoscopic palliative therapy in neoplastic diseases of the esophagus.

    abstract::Over the past 20 years, fiberoptic endoscopy, followed by video-endoscopy, has taken on an increasingly important role in the diagnostics and therapy for digestive tract diseases. Especially in the field of oncology, endoscopy is fundamental not only for the diagnosis and staging of diseases of the gastroenteric tract...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Casale V,Lapenta R,Gigliozzi A,Villotti G

    更新日期:1999-03-01 00:00:00

  • A synthetic cell-penetrating peptide derived from nuclear localization signal of EPS8 exerts anticancer activity against acute myeloid leukemia.

    abstract:BACKGROUND:Oncogenic roles of epidermal growth factor receptor pathway substrate no.8 (EPS8) have been widely reported in various tumors, making targeting of EPS8 an appealing prospect. Here, we describe the role of EPS8 in acute myeloid leukemia (AML) and consider the potential of EPS8 as an anti-AML target. Nuclear l...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-018-0682-x

    authors: Chen Y,Xie X,Wu A,Wang L,Hu Y,Zhang H,Li Y

    更新日期:2018-01-22 00:00:00

  • Effect of bacterial metabolism in the intestine on colorectal tumors induced by 1,2-dimethylhydrazine in transgenic mice harboring human prototype c-Ha-ras genes.

    abstract::The number of colorectal tumors per mouse induced by 1,2-dimethylhydrazine in transgenic (Tg) mice carrying human c-Ha-ras genes was significantly reduced by ingestion of apple pectin (AP) or a culture condensate of Bifidobacterium longum(MB) compared with a control diet and non-Tg mice. However, there were no differe...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Ohno K,Narushima S,Takeuchi S,Itoh K,Itoh T,Hioki K,Nomura T

    更新日期:2001-03-01 00:00:00

  • Linc-ROR promotes esophageal squamous cell carcinoma progression through the derepression of SOX9.

    abstract:BACKGROUND:Novel therapies tailored to the molecular composition of esophageal squamous cell carcinoma (ESCC) are needed to improve patient survival. We investigated the regulatory network of long intergenic non-protein coding RNA, regulator of reprogramming (linc-ROR) and sex-determining region Y-box 9 (SOX9), and the...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0658-2

    authors: Wang L,Yu X,Zhang Z,Pang L,Xu J,Jiang J,Liang W,Chai Y,Hou J,Li F

    更新日期:2017-12-13 00:00:00

  • Nuclear accumulation of UBC9 contributes to SUMOylation of lamin A/C and nucleophagy in response to DNA damage.

    abstract:BACKGROUND:Macroautophagy (hereafter referred to as autophagy) is an evolutionarily conserved intracellular mechanism for lysosomal degradation of damaged cellular components. The specific degradation of nuclear components by the autophagy pathway is called nucleophagy. Most studies have focused on autophagic turnover ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1048-8

    authors: Li Y,Jiang X,Zhang Y,Gao Z,Liu Y,Hu J,Hu X,Li L,Shi J,Gao N

    更新日期:2019-02-11 00:00:00

  • Correlation between peri-operative serum lactate levels and outcome in pancreatic resection for pancreatic cancer, preliminary report.

    abstract::This study was undertaken in order to verify if peri-operative serum lactate level changes, resulting from manipulation of the splanchnic circulation during pancreatectomy, reflected clinical outcome in twenty patients (9 males-11 females age 33 to 76) operated for pancreatic cancer. Lactate levels were evaluated at t...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Gruttadauria S,Marino IR,Vitale CH,Mandala L,Scott VL,Doria C

    更新日期:2002-12-01 00:00:00

  • Down-regulated miR-9 and miR-433 in human gastric carcinoma.

    abstract:BACKGROUND:MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors. METHODS:To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miR...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-28-82

    authors: Luo H,Zhang H,Zhang Z,Zhang X,Ning B,Guo J,Nie N,Liu B,Wu X

    更新日期:2009-06-16 00:00:00

  • Extent of spontaneous apoptosis in gastric cancer: relation to proliferative index, p53 expression, CD 34 expression and histopathological features.

    abstract::Gastric cancer, characterized by poor prognosis, remains a global health problem. Thus, it is important to describe the biological factors which can affect the prognosis in this disorder. The aim of our study was the determination of the relationship between apoptotic index (AI) and other clinicopathological features ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Jesionek-Kupnicka D,Tenderenda M,Rutkowski P

    更新日期:2002-09-01 00:00:00

  • Challenges and potential of PD-1/PD-L1 checkpoint blockade immunotherapy for glioblastoma.

    abstract::PD-1/PD-L1 checkpoint blockades have achieved significant progress in several kinds of tumours. Pembrolizumab, which targets PD-1, has been approved as a first-line treatment for advanced non-small cell lung cancer (NSCLC) patients with positive PD-L1 expression. However, PD-1/PD-L1 checkpoint blockades have not achie...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-019-1085-3

    authors: Wang X,Guo G,Guan H,Yu Y,Lu J,Yu J

    更新日期:2019-02-18 00:00:00

  • Radioguided occult lesion localization (ROLL) and excision of breast lesions using technetium-99m-macroaggregate albumin and air injection control.

    abstract::The aim of this study was to assess the efficiency of the radioguided localization and excision technique using radiopharmaceuticals injected directly or close to occult breast lesions. We studied thirty-two consecutive patients with thirty-six occult breast lesions detected mammographically or ultrasonically categori...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 临床试验,杂志文章

    doi:

    authors: Machado RH,Oliveira AC,Rocha AC,Landesmann MC,Martins FP,Lopes SA,Gutfilen B,da Fonseca LM

    更新日期:2007-09-01 00:00:00

  • Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases.

    abstract:BACKGROUND:Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-014-0119-0

    authors: Cordiali-Fei P,Trento E,Giovanetti M,Lo Presti A,Latini A,Giuliani M,D'Agosto G,Bordignon V,Cella E,Farchi F,Ferraro C,Lesnoni La Parola I,Cota C,Sperduti I,Vento A,Cristaudo A,Ciccozzi M,Ensoli F

    更新日期:2015-01-16 00:00:00

  • Role of retinoic receptors in lung carcinogenesis.

    abstract::Several in vitro and in vivo studies have examined the positive and negative effects of retinoids (vitamin A analogs) in premalignant and malignant lesions. Retinoids have been used as chemopreventive and anticancer agents because of their pleiotropic regulator function in cell differentiation, growth, proliferation a...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-27-18

    authors: Bogos K,Renyi-Vamos F,Kovacs G,Tovari J,Dome B

    更新日期:2008-07-14 00:00:00

  • PERK/NRF2 and autophagy form a resistance mechanism against G9a inhibition in leukemia stem cells.

    abstract:BACKGROUND:The histone methyltransferase G9a has recently been identified as a potential target for epigenetic therapy of acute myeloid leukemia (AML). However, the effect of G9a inhibition on leukemia stem cells (LSCs), which are responsible for AML drug resistance and recurrence, is unclear. In this study, we investi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01565-3

    authors: Jang JE,Eom JI,Jeung HK,Chung H,Kim YR,Kim JS,Cheong JW,Min YH

    更新日期:2020-04-15 00:00:00

  • Adult genitourinary sarcomas: a report of seventeen cases and review of the literature.

    abstract::Seventeen adult urologic sarcomas treated at the Oncology Hospital of Ankara in the last ten-year period are described. There were six cases of paratesticular rhabdomyosarcoma (RMS), four cases of liposarcoma (LS), three cases of leiomyosarcoma (LMS); two cases of Kaposi sarcoma limited to glans penis, and only one pr...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Berkmen F,Celebioğlu AS

    更新日期:1997-03-01 00:00:00

  • Downregulation of CLDN7 due to promoter hypermethylation is associated with human clear cell renal cell carcinoma progression and poor prognosis.

    abstract:BACKGROUND:Metastasis is the primary cause of death in renal cell carcinoma (RCC). Loss of cell-to-cell adhesion, including tight junctions (TJs) is the initial step in the process of metastasis. Claudin-7 (CLDN7) is a major component of TJs. However, the clinical significance and its regulation of kidney tumorigenesis...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-018-0924-y

    authors: Li Y,Gong Y,Ning X,Peng D,Liu L,He S,Gong K,Zhang C,Li X,Zhou L

    更新日期:2018-11-14 00:00:00

  • A SUMOylation-dependent HIF-1α/CLDN6 negative feedback mitigates hypoxia-induced breast cancer metastasis.

    abstract:BACKGROUND:We have previously described CLDN6 as a tumor suppressor gene in breast cancer. Here, a new finding is that CLDN6 was upregulated under hypoxia, a commonly recognized factor that promotes tumor metastasis. In this study, we aim to explain this confusing finding and delineate the role of CLDN6 in the breast c...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01547-5

    authors: Jia Y,Guo Y,Jin Q,Qu H,Qi D,Song P,Zhang X,Wang X,Xu W,Dong Y,Liang Y,Quan C

    更新日期:2020-02-24 00:00:00

  • Evaluation of HER2 and p53 expression in predicting response to docetaxel-based first-line chemotherapy in advanced breast cancer.

    abstract:BACKGROUND:The human epidermal growth factor receptor 2 (HER2) and p53 pathways may be involved in chemotherapy sensitivity and/or resistance. We explore the value of HER2 and p53 status to foretell docetaxel sensitivity in advanced breast cancer. METHODS:HER2 and p53 expression was analysed in 36 (median age 55 yrs; ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-38

    authors: Camerini A,Donati S,Viacava P,Siclari O,Puccetti C,Tartarelli G,Valsuani C,De Luca F,Martini L,Cavazzana A,Amoroso D

    更新日期:2011-04-11 00:00:00

  • Mucin 17 inhibits the progression of human gastric cancer by limiting inflammatory responses through a MYH9-p53-RhoA regulatory feedback loop.

    abstract:BACKGROUND:Mucins are key components of the mucosal barrier in the stomach that protects epithelia from carcinogenic effects of chronic inflammation. Analysis of The Cancer Genome Atlas database indicated that mucin-17 (MUC17) was more highly expressed in gastric cancer (GC) specimens, with favourable prognosis for pat...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1279-8

    authors: Yang B,Wu A,Hu Y,Tao C,Wang JM,Lu Y,Xing R

    更新日期:2019-07-01 00:00:00

  • microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis.

    abstract:BACKGROUND:Colorectal cancer (CRC) is one of the frequently occurred malignancies in the world. To date, several onco-microRNAs (miRNAs or miRs), including miR-96, have been identified in the pathogenesis of CRC. In the present study, we aimed to corroborate the oncogenic effect of miR-96 on CRC and to identify the spe...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01731-7

    authors: Yue C,Chen J,Li Z,Li L,Chen J,Guo Y

    更新日期:2020-11-12 00:00:00

  • Oncogene addiction in gliomas: implications for molecular targeted therapy.

    abstract::Oncogene addiction is a phenomenon that the survival of cancer cells depends on an activated oncogene or inactivation of tumor suppressor gene, and is regarded as the 'Achilles heel' of the successful molecular targeted therapies in cancer. However, the role of oncogene addiction in gliomas has not been elucidated sys...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-30-58

    authors: Yan W,Zhang W,Jiang T

    更新日期:2011-05-17 00:00:00

  • Gut-endocrinomas (carcinoids and related endocrine variants) of the uterine cervix: an analysis of 205 reported cases.

    abstract::This study was carried out statistically to evaluate the present situation of carcinoids and related endocrine variants in the uterine cervix. A total of 205 patients who had been reported on in world-wide literature were divided into two groups; one, as a carcinoid group, consisting of 81 patients with carcinoids, 49...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Soga J,Osaka M,Yakuwa Y

    更新日期:2001-09-01 00:00:00

  • Cisplatin triggers cancer stem cell enrichment in platinum-resistant cells through NF-κB-TNFα-PIK3CA loop.

    abstract:BACKGROUND:Parallel to complex alteration in molecular and cellular events, enrichment of cancer stem cells (CSC) contributes significantly in deliberation and maintenance of cisplatin resistance. Cisplatin mediated CSC enrichment is well established in various cancers, yet the underlying mechanism is largely unknown. ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0636-8

    authors: Thakur B,Ray P

    更新日期:2017-11-23 00:00:00

  • Serum soluble Fas antigen in gastric MALT (mucosa-associated lymphoid tissue) lymphoma patients.

    abstract::We measured the serum level of soluble Fas (sFas) antigen in gastric MALT (mucosa-associated lymphoid tissue) lymphoma patients to investigate immune response of B lymphoma cells in gastric MALT lymphoma. The serum sFas antigen levels in 21 patients with Helicobacter pylori (HP)-positive MALT lymphoma were 4.51+/-0.25...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Doi T,Nishikawa Y,Endo H,Hosokawa Y,Tanimizu M,Hyodo I

    更新日期:1999-09-01 00:00:00

  • Alterations of dendritic cell subsets in the peripheral circulation of patients with cervical carcinoma.

    abstract::Patients with cervical carcinoma (CC) are frequently immunocompromised. Dendritic cells (DCs) are potent antigen-presenting cells. Using multicolor flow cytometry, the percentages of CD11c+ (DC1) and CD123+ (DC2) subsets, were determined in the peripheral blood of 37 patients with cervical carcinoma (CC), 54 patients ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1756-9966-29-78

    authors: Ye F,Yu Y,Hu Y,Lu W,Xie X

    更新日期:2010-06-18 00:00:00

  • PD-L1 expression and tumor mutational burden status for prediction of response to chemotherapy and targeted therapy in non-small cell lung cancer.

    abstract:BACKGROUND:Several targeted immunotherapies have recently showed significant advances in treatment of non-small cell lung cancer (NSCLC), including antibodies and inhibitors targeting programmed death-1 (PD-1) and its ligand (PD-L1). METHODS:Tumor tissue samples were prospectively collected from 183 patients with NSCL...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1192-1

    authors: Chen Y,Liu Q,Chen Z,Wang Y,Yang W,Hu Y,Han W,Zeng H,Ma H,Dai J,Zhang H

    更新日期:2019-05-14 00:00:00

  • An imbalance in progenitor cell populations reflects tumour progression in breast cancer primary culture models.

    abstract:BACKGROUND:Many factors influence breast cancer progression, including the ability of progenitor cells to sustain or increase net tumour cell numbers. Our aim was to define whether alterations in putative progenitor populations could predict clinicopathological factors of prognostic importance for cancer progression. ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-45

    authors: Donatello S,Hudson L,Cottell DC,Blanco A,Aurrekoetxea I,Shelly MJ,Dervan PA,Kell MR,Stokes M,Hill AD,Hopkins AM

    更新日期:2011-04-26 00:00:00

  • sE-cadherin serves as a diagnostic and predictive parameter in prostate cancer patients.

    abstract:BACKGROUND:Measurement of prostate-specific antigen (PSA) advanced the diagnostic and prognostic potential for prostate cancer (PCa). However, due to PSA's lack of specificity, novel biomarkers are needed to improve risk assessment and ensure optimal personalized therapy. A set of protein molecules as potential biomark...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-015-0161-6

    authors: Tsaur I,Thurn K,Juengel E,Gust KM,Borgmann H,Mager R,Bartsch G,Oppermann E,Ackermann H,Nelson K,Haferkamp A,Blaheta RA

    更新日期:2015-05-14 00:00:00