Abstract：
:It has been reported that the activation of multiple myeloma (MM) cells by CD40 induces proliferation, growth arrest, and apoptosis. To determine whether the biologic sequelae of CD40 activation in MM cells depends on p53 function, we identified temperature-sensitive p53 mutations in the RPMI 8226 (tsp53E285K) and the HS Sultan (tsp53Y163H) MM cell lines. These cells were then used as a model system of inducible wtp53-like function because wild-type-like p53 is induced at permissive (30 degrees C) but not at restrictive (37 degrees C) temperatures. Using p21-luciferase reporter assays, we confirmed that CD40 induces p53 transactivation in RPMI 8226 and HS Sultan cells cultured under permissive, but not restrictive, conditions. Furthermore, CD40 activation of these MM cells under permissive, but not restrictive, temperatures increased the expression of p53 and p21 mRNA and protein. Importantly, CD40 activation induced the proliferation of RPMI 8226 and HS Sultan cells at restrictive temperatures and growth arrest and increased subG1 phase cells at permissive temperatures. These data confirmed that CD40 activation might have distinct biologic sequelae in MM cells, depending on their p53 status.
journal_name
Bloodjournal_title
Bloodauthors
Teoh G,Tai YT,Urashima M,Shirahama S,Matsuzaki M,Chauhan D,Treon SP,Raje N,Hideshima T,Shima Y,Anderson KCsubject
Has Abstractpub_date
2000-02-01 00:00:00pages
1039-46issue
3eissn
0006-4971issn
1528-0020journal_volume
95pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Natural killer (NK) cells participate in host defense by surveying for and ultimately killing virally infected or malignant target cells. NK cell cytotoxicity is a tightly regulated process that proceeds stepwise from adhesion and activation to the secretion of preformed lytic granule contents onto a diseased or stres...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-06-437012
更新日期：2013-04-04 00:00:00
abstract：:Omenn syndrome (OS) is a severe immunodeficiency associated with erythroderma, lymphoproliferation, elevated IgE, and hyperactive oligoclonal T cells. A restricted T-cell repertoire caused by defective thymic T-cell development and selection, lymphopenia with homeostatic proliferation, and lack of regulatory T cells a...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2015-03-631374
更新日期：2015-10-01 00:00:00
abstract：:The cellular immune defect in untreated Hodgkin's disease (HD) has long been recognized. This defect appears to be responsible for at least some of the morbidity and ultimately the mortality associated with the disease. In recent years, many studies have shown that the T cell component of the immune response is the ap...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Most anticancer chemotherapies are immunosuppressive and induce nonimmunogenic tumor cell death. Bortezomib, a specific inhibitor of 26S proteasome, has shown clinical activity in several human tumors, including myeloma. Here we show that the uptake of human myeloma cells by dendritic cells (DCs) after tumor cell deat...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-10-054221
更新日期：2007-06-01 00:00:00
abstract：:To evaluate the relationship between myeloperoxidase (MPO) gene expression and specific lineages of hematopoietic differentiation, we analyzed expression of the MPO gene in a variety of normal and leukemic human cells. As a first step, we synthesized several oligonucleotide probes and isolated cDNA clones for the MPO ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:Murine bone marrow cells were fractionated by fluorescence-activated cell sorting into Rh123lo Lin- c-kit+ Ly6A+, Rh123hi Lin-c-kit+ Ly6A+, and Lin- c-kit+ Ly6A- populations within which most, if not all, of the hematopoietic activities of the marrow resided. The Rh123lo Lin- c-kit+Ly6A+ cells, which consist exclusive...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-03-15 00:00:00
abstract：:We evaluated a treatment strategy targeting both lymphoma cells (by rituximab) and the microenvironment (by thalidomide) in 16 patients with relapsed/refractory mantle cell lymphoma (MCL). Rituximab was administered at 375 mg/m(2) for 4 weekly doses concomitantly with thalidomide (200 mg daily, with a dose increment t...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2004-03-1091
更新日期：2004-10-15 00:00:00
abstract：:The translocation t(8;14)(q24;q32) is the characteristic chromosomal aberration of Burkitt's-type lymphomas and leukemias (BLs). On the molecular level, the t(8;14) juxtaposes the c-myc gene in 8q24 next to the IgH locus in 14q32, resulting in overexpression of the transcription factor c-Myc. The detection of a t(8;14...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Polymorphonuclear leukocytes (PMNLs) exposed to influenza A virus (IAV) undergo activation of the respiratory burst followed by depression of cell function when subsequently exposed to particulate or soluble stimuli. The effect of IAV on PMNLs is likely to be mediated through the attachment of IAV to one or more speci...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-03-15 00:00:00
abstract：:Noncovalent association between the von Willebrand factor (VWF) propeptide (VWFpp) and mature VWF aids N-terminal multimerization and protein compartmentalization in storage granules. This association is currently thought to dissipate after secretion into blood. In the present study, we examined this proposition by qu...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-10-387548
更新日期：2012-05-17 00:00:00
abstract：:Plasma glutathione peroxidase (GSHPx) (glutathione: H2O2 oxidoreductase) is a unique selenoglycoprotein. Treatment of this enzyme with glycopeptidase F partially deglycosylates it and establishes the presence of N-linked sugar moieties. Antibodies raised in a rabbit against the purified enzyme from plasma were found t...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:CD34+ cells devoid of detectable mature and immature T and B lymphocytes, expressing the CD2, CD10, and CD20 antigens, were isolated from marrows of three pairs of sex-mismatched, mixed lymphocyte culture (MLC) nonreactive, sibling baboons. Reciprocal transplants were performed between members of each pair, using the ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:A rapid sensitive spectrophotometric assay for the measurement of methylmalonic and propionic acids in urine is described. The assay is based upon the quantitation of propionic acid using acetyl coenzyme A synthetase isolated from baker's yeast. This enzyme is highly specific for acetate and propionate, and acetate in...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Cellular oxygen partial pressure is sensed by a family of prolyl-4-hydroxylase domain (PHD) enzymes that modify hypoxia-inducible factor (HIF)alpha subunits. Upon hydroxylation under normoxic conditions, HIFalpha is bound by the von Hippel-Lindau tumor suppressor protein and targeted for proteasomal destruction. Since...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-10-3980
更新日期：2005-06-15 00:00:00
abstract：:Exposure to ambient particulate matter (PM) air pollution has been reported to trigger inflammation and thrombosis. However, molecular mechanisms underlying the modulation of coagulation pathways in PM-induced thrombosis remain largely unknown. We report here that Sirt1, a member of class III histone deacetylase, cont...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-04-350413
更新日期：2012-03-08 00:00:00
abstract：:Migration to lymph nodes and secretion of cytokines are critical functions of mature dendritic cells (DCs); however, these 2 functions are not necessarily linked. This is the first report showing that quantitative differences in identical signaling pathways determine DC migration and cytokine secretion. Using differen...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-12-4146
更新日期：2004-08-15 00:00:00
abstract：:Human neutrophil elastase (NE), a 29-Kd potent serine protease stored in azurophilic granules of mature neutrophils, is coded for by the NE gene, a single copy gene with 5 exons spanning a 6-kb segment of chromosome 11 at q14. With the knowledge that the NE gene expression is limited to early myeloid cell differentiat...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-05-15 00:00:00
abstract：:We used functional expression cloning in mammalian cells to identify a cDNA clone encoding a hematopoietic growth factor that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, MO7E. Analysis of the sequence of this cDNA revealed striking similarity to that of a recently reported novel mu...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:Understanding the repopulating characteristics of human hematopoietic stem/progenitor cells is crucial for predicting their performance after transplant into patients receiving high-dose radiochemotherapy. We have previously reported that CD34(+) cord blood (CB) cells can be expanded in vitro for several months in ser...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:The value of a postremission treatment in acute myelogenous leukemia (AML), with alternating combinations of non-cross-resistant drugs, has been prospectively assessed. Of 515 evaluable patients, 347 (67.4%) entered into complete remission (CR), following induction treatment with daunorubicin (DNR), vincristine (VCR),...
journal_title：Blood
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1989-03-01 00:00:00
abstract：:Bcl-2 protein expression has been associated with poor prognosis in patients with noncutaneous diffuse large B-cell lymphomas. In primary cutaneous large B-cell lymphomas, the location on the leg, the round-cell morphology defined as the predominance of centroblasts and immunoblasts over large centrocytes, and multipl...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：10.1182/blood-2003-08-2726
更新日期：2004-05-15 00:00:00
abstract：:2B4 belongs to the CD2 subset of the IgG family of receptors. Members in this family have been shown to function as coreceptors via homophilic or heterophilic interactions. Both 2B4 and CD2 bind to CD48, another member of this family. Because all 3 molecules are expressed on natural killer (NK) cells, it raises a poss...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-01-0185
更新日期：2006-04-15 00:00:00
abstract：:Chromosome 7 translocations, deletions, or monosomy are associated with myelodysplasia (MDS) and acute myeloid leukemia both in children and adults. These chromosomal anomalies represent one of the most common cytogenetic abnormalities associated with these diseases and usually herald a poor prognosis. In this study t...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:The introduction of the Bruton tyrosine kinase (BTK) inhibitor ibrutinib has dramatically changed the management of chronic lymphocytic leukemia (CLL). Although responses have been durable in the majority of patients, relapses do occur, especially in the high-risk patient population. Most relapses occur as the result ...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2016-09-693598
更新日期：2017-03-09 00:00:00
abstract：:A unique subset of CD86(-) HSCs was previously discovered in mice that were old or chronically stimulated with lipopolysaccharide. Functionally defective HSCs were also present in those animals, and we now show that CD86(-) CD150(+) CD48(-) HSCs from normal adult mice are particularly poor at restoring the adaptive im...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-10-388736
更新日期：2012-05-24 00:00:00
abstract：:C3H mice have higher average ploidy megakaryocytes than all other mouse strains tested, but the mode of inheritance of this anomaly is unknown. Therefore, to clarify the genetics of high ploidy megakaryocytes in C3H mice, we measured megakaryocyte DNA content from both male and female offspring from F1, as well as bac...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-03-15 00:00:00
abstract：:We examined TP53 mutation in 57 patients with myelodysplastic syndrome (MDS) at either the MDS phase or at the terminal leukemic phase using polymerase chain reaction-mediated single-strand conformation polymorphism (PCR-SSCP) analysis. TP53 mutations within exons 5 through 8 were found in seven patients. All these mu...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-04-15 00:00:00
abstract：:Pseudomonas aeruginosa pneumonia was produced in dogs with radiation-induced leukopenia to study the comparative efficacy of several different therapies. In a randomized control trial, five treatment regimens were compared: no antibiotics or granulocytes (controls), gentamicin (5 mg/kg/day), carbenicillin (500 mg/kg/d...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:We evaluated spleen function in 193 children with sickle cell anemia 8 to 18 months of age by (99m)Tc sulfur-colloid liver-spleen scan and correlated results with clinical and laboratory parameters, including 2 splenic biomarkers: pitted cell counts (PIT) and quantitative Howell-Jolly bodies (HJB) enumerated by flow c...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2010-04-278747
更新日期：2011-03-03 00:00:00
abstract：:Sickle red cell (SS RBC) adhesion is believed to contribute to the process of vaso-occlusion in sickle cell disease (SCD). We previously found that the LW RBC adhesion receptor can be activated by epinephrine to mediate SS RBC adhesion to endothelial alphavbeta3 integrin. To determine the contribution of LW activation...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-11-056101
更新日期：2007-10-01 00:00:00
