Assignment of cytosine N3 resonances in nucleic acids via intrabase three-bond coupling to amino protons.

Abstract:

:Coherences were observed between 15N3 of cytosine and its trans amino proton (H42) using a modified gradient-based heteronuclear single quantum coherence (HSQC) pulse sequence optimized for three-bond proton-nitrogen couplings. The method is demonstrated with a 22-nucleotide RNA fragment of the P5abc region of a group I intron uniformly labeled with 15N. Use of intraresidue 15N3-amino proton couplings to assign cytosine 15N3 signals complements the recently proposed JNN HNN COSY [Dingley, A.J. and Grzesiek, S. (1998) J. Am. Chem. Soc., 120, 8293-8297] method of identifying hydrogen-bonded base pairs in RNA.

journal_name

J Biomol NMR

authors

Rüdisser S,Pelton JG,Tinoco I Jr

doi

10.1023/a:1008359723515

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

173-6

issue

2

eissn

0925-2738

issn

1573-5001

journal_volume

15

pub_type

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