Abstract:
:Coherences were observed between 15N3 of cytosine and its trans amino proton (H42) using a modified gradient-based heteronuclear single quantum coherence (HSQC) pulse sequence optimized for three-bond proton-nitrogen couplings. The method is demonstrated with a 22-nucleotide RNA fragment of the P5abc region of a group I intron uniformly labeled with 15N. Use of intraresidue 15N3-amino proton couplings to assign cytosine 15N3 signals complements the recently proposed JNN HNN COSY [Dingley, A.J. and Grzesiek, S. (1998) J. Am. Chem. Soc., 120, 8293-8297] method of identifying hydrogen-bonded base pairs in RNA.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Rüdisser S,Pelton JG,Tinoco I Jrdoi
10.1023/a:1008359723515subject
Has Abstractpub_date
1999-10-01 00:00:00pages
173-6issue
2eissn
0925-2738issn
1573-5001journal_volume
15pub_type
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