Sample preparation and storage can change arsenic speciation in human urine.

abstract：:We compared three current methods (immunoinhibition, "Isomune-CK" immunoprecipitation, and the Tandem-E CKMB II immunoenzymometric assay) for determination of creatine kinase (CK; EC 2.7.3.2) isoenzyme MB in serum. Although results inter-correlated well, the immunoinhibition assay gave higher activity values. Atypical...

journal_title：Clinical chemistry

authors： Delanghe JR,De Mol AM,De Buyzere ML,De Scheerder IK,Wieme RJ

更新日期：1990-01-01 00:00:00

abstract：:The historical development of a charitable children's hospital and the evolution of its clinical laboratory are presented. With the appearance of practical quantitative blood chemistry tests in the period between the two World Wars, applications to pediatrics were hampered by the need for ultramicro procedures then un...

journal_title：Clinical chemistry

authors： Meites S

更新日期：2000-07-01 00:00:00

abstract：BACKGROUND:The B-type natriuretic peptides (BNP and N-terminal pro-BNP) are secreted by the heart and, in the case of BNP, serve to maintain circulatory homeostasis through renal and vascular actions and oppose many effects of the renin-angiotensin system. Recent evidence suggests that in patients with severe heart fai...

journal_title：Clinical chemistry

authors： Mahagamasekera PG,Ruygrok PN,Palmer SC,Richards AM,Ansell GS,Nicholls MG,Pemberton CJ,Lewis LK,Yandle TG

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification h...

journal_title：Clinical chemistry

authors： Daher RK,Stewart G,Boissinot M,Bergeron MG

更新日期：2014-04-01 00:00:00

abstract：:We present data on a filter-paper collection and assay method for measurement of glycated hemoglobin (gHb). Onto filter paper dipped into a solution of glucose oxidase (EC 1.1.3.4) and allowed to dry, approximately 20 microL of capillary blood was spotted. For analysis, we eluted the dried blood spot from the paper by...

journal_title：Clinical chemistry

authors： Little RR,McKenzie EM,Wiedmeyer HM,England JD,Goldstein DE

更新日期：1986-05-01 00:00:00

abstract：:We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working li...

journal_title：Clinical chemistry

authors： Tomokuni K,Ichiba M,Hirai Y,Hasegawa T

更新日期：1987-09-01 00:00:00

abstract：:One currently observes many healthcare institutions rushing to reengineer and install information systems with the expectation of achieving enhanced efficiency, competitiveness, and, it is hoped, higher patient satisfaction resulting from timely, high-quality care. Unfortunately, information system concepts, design, a...

journal_title：Clinical chemistry

authors： Buffone GJ,Moreau DR

更新日期：1995-09-01 00:00:00

abstract：:We describe a rapid polarization fluoroimmunoassay for screening for the presence of barbiturates in urine. The single reagent is prepared by pre-mixing antiserum with a fluorescein-labeled barbiturate derivative as tracer. Use of a mixture of two sheep antisera, raised against different barbiturate immunogens, result...

journal_title：Clinical chemistry

authors： Colbert DL,Smith DS,Landon J,Sidki AM

更新日期：1984-11-01 00:00:00

abstract：:Quantification of ethanol by a radiative energy attenuation (REA) technique was evaluated and compared with results by gas-liquid chromatography (GLC) and by the Du Pont aca. Within-assay CVs were less than 5.5%. Between-assay CVs ranged from 1.9% to 6.0% for serum and blood controls at concentrations of 0.5, 1.0, and...

journal_title：Clinical chemistry

authors： Cary PL,Whitter PD,Johnson CA

更新日期：1984-11-01 00:00:00

abstract：BACKGROUND:Unpredictable efficacy and toxicity are hallmarks of most anticancer therapies. Predictive markers are factors that are associated with response or resistance to a particular therapy. METHODS:The English literature relating to predictive markers in oncology was reviewed. Particular attention was paid to met...

journal_title：Clinical chemistry

authors： Duffy MJ

更新日期：2005-03-01 00:00:00

abstract：:This relatively simple, rapid method for quantification of homovanillic acid (HVA) and vanillylmandelic acid (VMA) involves solvent extraction and capillary gas chromatography. With use of this extraction method, the overall analytical recovery from an aqueous solution is 92.5% for HVA and 79.2% for VMA. 3,4-Dihydroxy...

journal_title：Clinical chemistry

authors： Tuchman M,Crippin PJ,Krivit W

更新日期：1983-05-01 00:00:00

abstract：:We evaluated the performance of two homogeneous assays for quantifying HDL cholesterol (HDL-C) and compared them with the phosphotungstic acid (PTA)/ MgCl2 assay. Both homogeneous HDL-C assays were precise, having a within-run CV of < 1.20% and a between-run CV of < 4.07%. The HDL-C values (y) measured by the two homo...

journal_title：Clinical chemistry

authors： Huang YC,Kao JT,Tsai KS

更新日期：1997-06-01 00:00:00

abstract：BACKGROUND:Proteomic technology permits the investigation of genetic metabolic diseases at the level of protein expression. Changes in the expression, polypeptide structure, and posttranslational modification of individual proteins can be detected in complex mixtures of proteins. METHODS:We used high-resolution two-di...

journal_title：Clinical chemistry

authors： Mills K,Mills PB,Clayton PT,Johnson AW,Whitehouse DB,Winchester BG

更新日期：2001-11-01 00:00:00

abstract：BACKGROUND:The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS:We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLi...

journal_title：Clinical chemistry

authors： Wenzel JJ,Walch H,Bollwein M,Niller HH,Ankenbauer W,Mauritz R,Höltke HJ,Zepeda HM,Wolf H,Jilg W,Reischl U

更新日期：2009-12-01 00:00:00

abstract：:We evaluated a new kinetic assay for alpha-amylase (Phadebas IsoAmylase Test), using modified starch as the substrate and a CentrifiChem 400 centrifugal analyzer system. We determined isoamylase activities by using a selective inhibitor. Results were compared with those obtained with the chromogenic Phadebas dyed-star...

journal_title：Clinical chemistry

authors： de Rijke D,Kreutzer HJ

更新日期：1983-06-01 00:00:00

abstract：:We have observed an electrophoretically abnormal, nonfunctional species of alpha 1-antitrypsin in serum from patients who were receiving bone-marrow transplants for treatment of leukemia or aplastic anemia. Three of four patients in whose serum this protein appeared died soon after; the fourth recovered, and the disap...

journal_title：Clinical chemistry

authors： Mullins RE,Bennett B,Hunter RL Jr

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND:The association between increases in cardiac troponin and adverse cardiac outcomes is well established. There is a growing interest in exploring routine cardiac troponin monitoring as a potential early indicator of adverse heart health trends. Prognostic use of cardiac troponin measurements requires an assay...

journal_title：Clinical chemistry

authors： Jarolim P,Patel PP,Conrad MJ,Chang L,Melenovsky V,Wilson DH

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND:Rapid detection of biological threat agents is critical for timely therapeutic administration. Fluorogenic PCR provides a rapid, sensitive, and specific tool for molecular identification of these agents. We compared the performance of assays for 7 biological threat agents on the Idaho Technology, Inc. R.A.P....

journal_title：Clinical chemistry

authors： Christensen DR,Hartman LJ,Loveless BM,Frye MS,Shipley MA,Bridge DL,Richards MJ,Kaplan RS,Garrison J,Baldwin CD,Kulesh DA,Norwood DA

更新日期：2006-01-01 00:00:00

abstract：:We describe a ratio gas-chromatographic method for determination of cortisol production rate by measuring the isotope dilution of urinary cortisol metabolites. The method was calibrated by analyzing [3H]tetrahydrocortisol and [3H]tetrahydrocortisone of known specific activities. Results are reasonably well reproducibl...

journal_title：Clinical chemistry

authors： Derks HJ,Muskiet FA,Wolthers BG,Thijssen JH,Drayer NM

更新日期：1977-03-01 00:00:00

abstract：:We describe a sensitive beta-endorphin (beta-EP) radioimmunoassay specific for beta-EP 1-31 applied to human ventricular and lumbar cerebrospinal fluid (CSF). Specificity was documented by reversed-phase HPLC of CSF pools. Simultaneous ventricular and lumbar CSF samples from 13 patients suspected of having normal-pres...

journal_title：Clinical chemistry

authors： Bach FW,Schmidt JF,Faber T

更新日期：1992-06-01 00:00:00

abstract：:To study the effect of fatty acid chain saturation on the fluorescence polarization assay as a measure of fetal lung maturity, we used purified phospholipids isolated from human amniotic fluid and various commercial phospholipids. We found that the fluorescence polarization value decreased as the concentration of unsa...

journal_title：Clinical chemistry

authors： Dohnal JC,Bowie LJ,Burstein HJ

更新日期：1986-03-01 00:00:00

abstract：BACKGROUND:Clinical assessment of vitamin D status often relies on measuring total circulating 25-hydroxyvitamin D3 (25OHD3), but much of each vitamin D metabolite is bound to plasma vitamin D-binding protein (DBP), such that the percentage of free vitamin is very low. We hypothesized that measurement of free rather th...

journal_title：Clinical chemistry

authors： Al-oanzi ZH,Tuck SP,Raj N,Harrop JS,Summers GD,Cook DB,Francis RM,Datta HK

更新日期：2006-02-01 00:00:00

abstract：BACKGROUND:The goal of this study was to establish a plasma microRNA profile by use of next-generation sequencing that could aid in assessment of patient prognosis in nasopharyngeal carcinoma (NPC). METHODS:Two panels of NPC patients and healthy controls (HCs) were recruited for this study. We used deep sequencing to ...

journal_title：Clinical chemistry

authors： Wang HY,Yan LX,Shao Q,Fu S,Zhang ZC,Ye W,Zeng YX,Shao JY

更新日期：2014-05-01 00:00:00

abstract：:We evaluated a new analyzer that measures lactate in undiluted whole blood by direct (or undiluted) amperometry [Nova Stat Profile 7 Analyzer (SP7); Nova Biomedical, Waltham, MA] by comparing it with two other analyzers, one for measuring lactate in whole blood by indirect (or diluted) amperometry [Model 2300; Yellow ...

journal_title：Clinical chemistry

authors： Toffaletti J,Hammes ME,Gray R,Lineberry B,Abrams B

更新日期：1992-12-01 00:00:00

abstract：:Measurements of alanine aminotransferase and aspartate aminotransferase with the SMAC were evaluated for correlation with the ABA-100, precision, linearity, and carryover. We assayed 200 specimens with normal and abnormal aminotransferase activities with both the SMAC (y) and the ABA-100 (X). Linear regression analysi...

journal_title：Clinical chemistry

authors： Purcell GV,Behenna DB,Walsh PR

更新日期：1979-05-01 00:00:00

abstract：:We describe a rapid, sensitive, and specific "high performance" liquid chromatographic analysis for disopyramide and its mono-N-dealkylated metabolite in serum, urine, and saliva. We used a mu-Bondapak CN column and an acetate buffer mobile phase containing methanol. Retention times for the two compounds and the inter...

journal_title：Clinical chemistry

authors： Lima JJ

更新日期：1979-03-01 00:00:00

abstract：:In this method for measuring amino acids in urine, serum, and tissue, the amino acids to be assayed and the internal standard, L-norleucine, are converted to phenylthiohydantoins , isolated by organic solvent extraction, separated by reversed-phase "high-pressure" liquid chromatography, and detected by ultraviolet abs...

journal_title：Clinical chemistry

authors： Biggs HG,Gentilcore LJ

更新日期：1984-06-01 00:00:00

abstract：:We describe a modification of the MacGee method [Anal. Chem. 42, 421 (1970)] for rapid determination of phenytoin (diphenylhydantoin) in plasma by gas-liquid chromatography, in which an ethylaing reagent is used instead of the more common methylating reagents. With this modification, several N-methylated antiepileptic...

journal_title：Clinical chemistry

authors： Friel P,Troupin AS

更新日期：1975-05-01 00:00:00

abstract：BACKGROUND:The next-generation, high-sensitivity cardiac troponin assays can measure quantifiable concentrations of cTn in a majority of individuals, but there are few studies assessing these assays for risk stratification. The present study was undertaken to determine if a research hs-cTnI assay can be useful for pred...

journal_title：Clinical chemistry

authors： Kavsak PA,Wang X,Ko DT,MacRae AR,Jaffe AS

更新日期：2009-10-01 00:00:00

abstract：:We report the presence of complexes between aspartate aminotransferase (AST, EC 2.6.1.1) and immunoglobulin (Ig) in the serum of a patient suffering from lung cancer with metastasis to the liver. After fractionation of the serum by gel filtration, AST-Ig complexes (AST-IgA, AST-IgG) were demonstrated by counterimmunoe...

journal_title：Clinical chemistry

authors： Nagamine M,Okochi K

更新日期：1983-02-01 00:00:00