Features of DNA fragments obtained by random amplified polymorphic DNA (RAPD) assays

Abstract:

:Random amplified polymorphic DNA (RAPD) fragments were prepared from samples of Calonectris diomedea (Cory's shearwater, Aves) and Haemonchus contortus (Nematoda) DNA by polymerase chain reaction (PCR) using decamers containing two restriction enzyme sites as primers. Six of 19 studied RAPD fragments probably originated from traces of commensal microorganisms. Many rearranged fragments, absent in the original genomic DNA, were synthesized and amplified during the processing of all the DNA samples, indicating that interactions occur within and between strands during the annealing step of PCR. The model of interactions between molecular species during DNA amplification with a single arbitrary oligonucleotide primer was modified to include nested primer annealing and interactions within and between strands. The presence of these artefacts in the final RAPD have a major effect on the interpretation of polymorphism studies.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Rabouam C,Comes AM,Bretagnolle V V,Humbert JF,Periquet G,Bigot Y

doi

10.1046/j.1365-294x.1999.00605.x

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

493-503

issue

3

eissn

0962-1083

issn

1365-294X

journal_volume

8

pub_type

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