Abstract:
BACKGROUND:Chronic hyperglycaemia in diabetes is suggested to be a major cause of diabetic angiopathy. Up until now, the effects of acutely induced hyperglycaemia in non-diabetic subjects as well as hyperglycaemic effects in early diabetes, on mesenteric microcirculation and leucocyte endothelial cell interaction have not been investigated extensively. METHODS:The aim of this study was to examine rat mesenteric microcirculatory parameters such as leucocyte adhesion, leucocyte emigration, venular shear rate and leucocyte rolling velocity using a new rat model both with continuous venous glucose infusion and with continuous arterial measurement of blood glucose concentration while observing mesenteric microcirculation with in vivo capillary microscopy in the non-diabetic and diabetic state. RESULTS:In normal non-diabetic rats, acute elevation of glucose concentration resulted in a highly significant, rapid and step-by- step enhancement of adhesion and emigration in a dose dependent manner. Leucocyte rolling velocity was reduced with rising glucose levels. Venular shear rate showed a similar reduction at all hyperglycaemic levels. In streptozotocin-induced diabetes, adhesion and emigration were significantly enhanced while shear rate and leucocyte rolling velocity were severely reduced, resembling the effects of glucose infusion experiments. Longer duration of diabetes resulted in a further enhancement of leucocyte adhesion and reduction of leucocyte rolling velocity while emigration and shear rate were not influenced by a longer period of diabetes manifestation. CONCLUSION:Experiments using different mannitol concentrations revealed that most of the observed glucose effects can be mimicked by mannitol and are therefore - at least in part - due to changes in osmolarity by yet unknown mechanisms.
journal_name
Eur J Clin Investjournal_title
European journal of clinical investigationauthors
Schäffler A,Arndt H,Schölmerich J,Palitzsch Kdoi
10.1046/j.1365-2362.1998.00390.xsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
886-93issue
11eissn
0014-2972issn
1365-2362journal_volume
28pub_type
杂志文章abstract:BACKGROUND:Memory and cognitive functions are known to decline with advancing age. Studies have suggested that this may be due to a decrease in cholinergic function in the brains of elderly people. This review aims to assess studies documented in the literature dealing with the 'scopolamine model' of dementia. METHODS...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2362.1998.00393.x
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Infiltration of white adipose tissue (WAT) by inflammatory cells in obesity is considered to be a key event in the development of insulin resistance. Recently, mast cells (MCs) have been identified as new players in the pathogenesis of obesity. We aimed to investigate the relationship between MCs and various...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12675
更新日期:2016-11-01 00:00:00
abstract::The aim of the study was to compare the changes in plasma renin activity induced by a vasodilator in normal dogs and in dogs with an impaired cardiac reserve. In normal conscious dogs, a 60-min nitroprusside infusion increased plasma renin activity from 1.05 +/- 0.26 to 8.35 +/- 1.20 ng, angiotensin I ml-1 h-1 (P less...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1983.tb00109.x
更新日期:1983-08-01 00:00:00
abstract::In order to study the mechanisms by which nutrients influence post-trauma metabolism, Sprague-Dawley rats received a 25% full-thickness burn and were randomly assigned to receive 12.5 g kg-1 bodyweight (BW) per day amino acids (AA) only, AA and 14.7 g hydrous glucose kg-1 BW per day or AA and 14.7 g hydrous xylitol kg...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1991.tb01818.x
更新日期:1991-04-01 00:00:00
abstract::Lipid and carbohydrate metabolism variables were studied in twenty-eight patients with chronic renal failure (mean GFR 7.7 +/- 2.5 ml/min) and uraemic symptoms. 71% of the patients had hypertriglyceridaemia (greater than or equal to 2.2 mmol/l). Total serum cholesterol was normal while VLDL cholesterol was high and al...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1979.tb00886.x
更新日期:1979-08-01 00:00:00
abstract:BACKGROUND:Excess of cardiovascular risk among patients with chronic inflammatory diseases has been attributed to increased arterial stiffness. Hypercholesterolaemia has been demonstrated to promote a low-grade inflammatory status. The objective of the present study was to define, in hypercholesterolaemia, the influenc...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2004.01345.x
更新日期:2004-05-01 00:00:00
abstract::It has been shown that the detection of tuberculostearic acid (TBSA) with gas chromatography-mass-spectrometry provides a highly specific, sensitive and rapid method for the diagnosis of various forms of tuberculosis. However, the need for complex and expensive equipment prevented the more widespread use of this metho...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1994.tb00975.x
更新日期:1994-02-01 00:00:00
abstract::The electrical parameters and the unidirectional fluxes of 45Ca and 3H-mannitol were measured in preparations of rat colon descendens freed from the muscularis externa and mounted in a modified Ussing-chamber. Two criteria were used to differentiate between changes in the trans- and the paracellular calcium transport ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1986.tb01007.x
更新日期:1986-10-01 00:00:00
abstract:BACKGROUND:To determine the influence of chorioamnionitis and neonatal sepsis on procalcitonin (PCT) levels in very-low-birth-weight (VLBW) infants within the first week of life. DESIGN:PCT serum levels were measured in cord blood 1 h after delivery and on day 3 and day 7 of life. Chorioamnionitis and neonatal sepsis ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.2001.00912.x
更新日期:2001-11-01 00:00:00
abstract:BACKGROUND:Both inflammation and oxidative stress may contribute to pathogenesis of metabolic syndrome (MetS). The C242T polymorphism (rs4673) in the CYBA gene, as the main components of NAD (P) H oxidase, causes inter-individual variability in the enzyme activity. We aimed to investigate the association between this p...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.13275
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Angiotensin converting enzyme 2 (ACE2), a monocarboxylase that degrades angiotensin II to angiotensin 1-7, is also the functional receptor for severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) and is highly expressed in the lungs and heart. Patients with SARS also suffered from cardiac disease ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2009.02153.x
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:Although the association between chronic Helicobacter pylori infection and type 2 diabetes has been suggested, findings have been inconsistent. This study evaluated the association between chronic H. pylori infection and glucose regulation. MATERIALS AND METHODS:We evaluated H. pylori infection status of pa...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12124
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:Genetic factors may be important in modifying heart size due to long-term athletic training. The significance of polymorphisms of genes of the renin-angiotensin system in myocardial mass in a population of athletes participating in different disciplines is not known. METHODS:The angiotensin I-converting enz...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.2001.00886.x
更新日期:2001-10-01 00:00:00
abstract::• The rapid and continuing progress in gene discovery for complex diseases is fuelling interest in the potential application of genetic risk models for clinical and public health practice. • The number of studies assessing the predictive ability is steadily increasing, but they vary widely in completeness of reporting...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2011.02493.x
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Inactivation of the tumour suppressor gene, p53, is the commonest genetic abnormality in human cancer. The study of the type of p53 mutation in a given tumour may provide prognostic information, clues to aetiology and become useful for therapeutics. MATERIALS AND METHODS:The molecular characterisation of p5...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.2000.00717.x
更新日期:2000-09-01 00:00:00
abstract::Urine was collected in four healthy infants, born after 35-39 weeks of gestation. The collections were made for 24 h starting immediately after birth. Bile acids were extracted, separated into conjugate groups, solvolysed, hydrolysed and quantified by gas-liquid chromatography as methyl ester trimethylsilyl ether deri...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1982.tb02236.x
更新日期:1982-08-01 00:00:00
abstract:BACKGROUND:The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular met...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12478
更新日期:2015-08-01 00:00:00
abstract::An increase in plasma renin activity (PRA) following experimental coronary occlusion has previously been demonstrated in anaesthetized and conscious dogs. The purpose of the present study was to analyse the mechanism of this renin release. In two distinct models of myocardial ischaemia in anaesthetized dogs--i.e. occl...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1985.tb00288.x
更新日期:1985-12-01 00:00:00
abstract::To understand better impairment of glucose utilization in diabetics during a hyperosmolal state, in vitro models were established to evaluate the effects of hyperosmolality on basal glucose uptake as well as glucagon dependent glucose release by isolated hepatocytes. In these studies simulating a hyperglycaemic (40 mm...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1989.tb00206.x
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:Data regarding the effect of vitamin C (VC) and vitamin E (VE) supplementation on insulin resistance in type 2 diabetes mellitus (T2DM) are controversial. We aimed to systematically review the current data on this topic. MATERIALS AND METHODS:All randomized controlled trials (RCTs) conducted to assess the e...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,meta分析
doi:10.1111/eci.12534
更新日期:2015-11-01 00:00:00
abstract::Assuming that lithium is exclusively reabsorbed in the proximal tubules pari passu with sodium (Na), the lithium clearance (CLi) has been advanced as an index of filtrate delivery from the proximal tubules. In order to determine whether water loads in a range commonly given during clearance studies affect CLi, we stud...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1988.tb01258.x
更新日期:1988-06-01 00:00:00
abstract::Currently available information on adipose tissue metabolism in man is mainly based on studies in vitro. In the present work adipose tissue lipid uptake was studied in vivo in man. 100 g glucose and 120 g milk fat with 10 microCi U-14C oleic acid were given in the overnight fasting state to eight women and 2 h after a...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1365-2362.1990.tb02263.x
更新日期:1990-04-01 00:00:00
abstract:BACKGROUND:RNA interference (RNAi) has become the method of choice for researchers wishing to target specific genes for silencing and has provided immense potential as therapeutic tools. This narrative review article aimed to understand potential benefits and limitations of RNAi technique for clinical application and i...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2362.2010.02400.x
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:Graves' disease (GD) is an autoimmune disorder characterized by hyperthyroidism, which can relapse in many patients after antithyroid drug treatment withdrawal. Several studies have been performed to predict the clinical course of GD in patients treated with antithyroid drugs, without conclusive results. The...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2004.01318.x
更新日期:2004-03-01 00:00:00
abstract::Pepsinogen A (PGA) and pepsinogen C (PGC) are negatively charged, low molecular weight (LMW) proteins with a striking difference in renal handling: PGA (molecular weight 43,500 daltons) shows a high fractional excretion while the fractional excretion of PGC (molecular weight 40,500 daltons) is low, presumably due to t...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1989.tb00234.x
更新日期:1989-06-01 00:00:00
abstract::There is considerable information to indicate that when there is vessel injury, platelets can play a major role in the development of atherosclerosis and its thromboembolic complications in the non-diabetic population. Diabetes is a disease associated with an increased risk of vascular complications, but the nature of...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2362.1994.tb02424.x
更新日期:1994-02-01 00:00:00
abstract::Plasma glucose concentration is the best predictor for the development of non-insulin-dependent diabetes mellitus (NIDDM). However, obesity is also a recognized risk factor for development of the disease, and is easier to track over time. Thus obesity could be of considerable clinical importance as a predictor of diab...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.1998.0280s2003.x
更新日期:1998-09-01 00:00:00
abstract::Thirty-one patients with IgG antibodies to cardiolipin (ACLA) were studied to determine their in vivo formation of the platelet aggregating and vasoconstricting substance thromboxane A2 (TxA2) and the platelet inhibiting and vasodilating substance prostacyclin (PGI2). This was done by measurements in urine of their en...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1990.tb01908.x
更新日期:1990-12-01 00:00:00
abstract:BACKGROUND:Nasal nitric oxide (nNO) values are reduced in patients with cystic fibrosis (CF). Humming during nNO measurement increases nNO values in healthy subjects. Nasal NO is reduced in patients with CF, sinus disease or nasal polyps. Humming nNO values have not been reported in CF patients yet. Our aim was to expl...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2007.01758.x
更新日期:2007-02-01 00:00:00
abstract::Although in therapeutic use for more than a century, the mode of cellular action of organic nitrates remains incompletely understood. Despite ample experimental evidence from animal studies to show that nitrates are metabolized to NO in the vascular smooth muscle, direct demonstration of such an activity in human vasc...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1995.tb01952.x
更新日期:1995-10-01 00:00:00