Abstract:
:We show herein the transcription of a processed gene that originated from a spliced transcript. Recently, we isolated the human and murine homologues of the yeast chromatin protein, SPT4. The Supt4h gene is spliced normally from five exons encoded by chromosome 11. Here we show that a related sequence on chromosome 10 encodes Supt4h2, a processed intronless gene (with a polyA tail and a tandemly-duplicated 13 bp insertion site in the genome) with a different 5' control region. Both the spliced gene, Supt4h , and the processed gene, Supt4h2 , are expressed in each of four tissues we examined. Supt4h2 encodes a 117 amino acid protein nearly identical to the Supt4h gene product with only one amino acid difference, indicating extreme conservation of this expressed processed gene with the spliced gene over evolutionary time. This illustrates another potential complexity of the mammalian genome, i.e. the use of a processed gene under the control of a different promoter region than the spliced gene.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chiang PW,Zhang R,Stubbs L,Zhang L,Zhu L,Kurnit DMdoi
10.1093/nar/26.21.4960subject
Has Abstractpub_date
1998-11-01 00:00:00pages
4960-4issue
21eissn
0305-1048issn
1362-4962pii
gkb782journal_volume
26pub_type
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