Abstract:
:We have isolated two DNA fragments from Sporothrix schenckii. These fragments were nonspecifically amplified from the whole-cell DNA using polymerase chain reaction (PCR) primers originally designed in the gene encoding heat shock protein 70 of Mycobacterium leprae. Reverse transcription PCR demonstrated that the smaller (sp-2) fragment was expressed, and a database search indicated that the deduced amino acids sequence from the sp-2 fragment contained a region homologous to the conserved sequence of the membrane transporter protein family. This is the first report of partial cloning of the gene encoding the putative membrane transporter in S. schenckii.
journal_name
Mycopathologiajournal_title
Mycopathologiaauthors
Sugita Y,Fukushima J,Murakami M,Takahashi I,Nakajima Hdoi
10.1023/a:1006999508574subject
Has Abstractpub_date
1998-01-01 00:00:00pages
101-4issue
2eissn
0301-486Xissn
1573-0832journal_volume
141pub_type
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