Identification of a DNA fragment encoding a putative membrane transporter protein expressed in Sporothrix schenckii.

Abstract:

:We have isolated two DNA fragments from Sporothrix schenckii. These fragments were nonspecifically amplified from the whole-cell DNA using polymerase chain reaction (PCR) primers originally designed in the gene encoding heat shock protein 70 of Mycobacterium leprae. Reverse transcription PCR demonstrated that the smaller (sp-2) fragment was expressed, and a database search indicated that the deduced amino acids sequence from the sp-2 fragment contained a region homologous to the conserved sequence of the membrane transporter protein family. This is the first report of partial cloning of the gene encoding the putative membrane transporter in S. schenckii.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Sugita Y,Fukushima J,Murakami M,Takahashi I,Nakajima H

doi

10.1023/a:1006999508574

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

101-4

issue

2

eissn

0301-486X

issn

1573-0832

journal_volume

141

pub_type

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