Use of D gene segments with irregular spacers in terminal deoxynucleotidyltransferase (TdT)+/+ and TdT-/- mice carrying a human Ig heavy chain transgenic minilocus.

abstract：:Replication of the kinetoplast DNA (kDNA) minicircle of trypanosomatids initiates at a conserved 12-nt sequence, 5'-GGGGTTGGTGTA-3', termed the universal minicircle sequence (UMS). A sequence-specific single-stranded DNA-binding protein from Crithidia fasciculata binds the heavy strand of the 12-mer UMS. Whereas this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Avrahami D,Tzfati Y,Shlomai J

更新日期：1995-11-07 00:00:00

abstract：:The spatiotemporal regulation of neurotransmitter transporters involves proteins that interact with their intracellular domains. Using a proteomic approach, we identified several proteins that interact with the C terminus of the serotonin transporter (SERT). These included neuronal nitric oxide synthase (nNOS), a PSD-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chanrion B,Mannoury la Cour C,Bertaso F,Lerner-Natoli M,Freissmuth M,Millan MJ,Bockaert J,Marin P

更新日期：2007-05-08 00:00:00

abstract：:The amino acid sequence of blastokinin, also called uteroglobin, has been determined by a combined study of both the intact native molecule and the peptide fragments resulting from tryptic and chymotryptic digestions. Sequence analyses performed by automated methods and by sequential digestion with leucine aminopeptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Popp RA,Foresman KR,Wise LD,Daniel JC Jr

更新日期：1978-11-01 00:00:00

abstract：:Unlike the dominant role of one class II invariant chain peptide (CLIP) in blocking MHC class II, comparative lipidomics analysis shows that human cluster of differentiation (CD) proteins CD1a, CD1b, CD1c, and CD1d bind lipids corresponding to hundreds of diverse accurate mass retention time values. Although most ions...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang S,Cheng TY,Young DC,Layre E,Madigan CA,Shires J,Cerundolo V,Altman JD,Moody DB

更新日期：2011-11-29 00:00:00

abstract：:Rational engineering methods can be applied with reasonable success to optimize physicochemical characteristics of proteins, in particular, antibodies. Here, we describe a combined CDR3 walking randomization and rational design-based approach to enhance the affinity of the human anti-gastrin TA4 scFv. The application ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barderas R,Desmet J,Timmerman P,Meloen R,Casal JI

更新日期：2008-07-01 00:00:00

abstract：:Mathematical models have been developed to explore the population dynamics of viral diseases among wildlife. However, assessing the predictions stemming from these models with wildlife databases adequate in size and temporal duration is uncommon. An epizootic of raccoon rabies that began in the mid-Atlantic region of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Childs JE,Curns AT,Dey ME,Real LA,Feinstein L,Bjørnstad ON,Krebs JW

更新日期：2000-12-05 00:00:00

abstract：:Mutations in the low density lipoprotein (LDL) receptor (LDLR) cause hypercholesterolemia because of inefficient LDL clearance from the circulation. In addition, there is a paradoxical oversecretion of the metabolic precursor of LDL, very low density lipoprotein (VLDL). We recently demonstrated that the LDLR mediates ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillian-Daniel DL,Bates PW,Tebon A,Attie AD

更新日期：2002-04-02 00:00:00

abstract：:Hoxb13 is robustly transcribed in derivatives of posterior endoderm including the colon, rectum, and the prostate gland. Transcriptional activity in the prostate persists unabated under conditions of androgen deprivation and throughout the course of disease progression in a mouse prostate cancer model. To elucidate th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMullin RP,Dobi A,Mutton LN,Orosz A,Maheshwari S,Shashikant CS,Bieberich CJ

更新日期：2010-01-05 00:00:00

abstract：:A rice Dwarf 1 gene was identified by using a map-based cloning strategy. Its recessive mutant allele confers a dwarf phenotype. Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3,185 d1 segregants. Southern hybridization analysis with this cDNA as a probe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ashikari M,Wu J,Yano M,Sasaki T,Yoshimura A

更新日期：1999-08-31 00:00:00

abstract：:31P nuclear magnetic relaxation rates for phosphocreatine in intact frog gastrocnemius were compared with those observed in model solutions at 4 degrees, a temperature at which muscle maintains its physiological state for at least 5 hr. Both nuclear Overhauser effect and spin-lattice relaxation rate (1/T1) experiments...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen SM,Burt CT

更新日期：1977-10-01 00:00:00

abstract：:Usual human livers contain two major aldehyde dehydrogenase [(ALDH) aldehyde:NAD+ oxidoreductase] isozymes--i.e., a cytosolic ALDH1 component and a mitochondrial ALDH2 component--whereas approximately equal to 50% of Orientals are "atypical" and have only the ALDH1 isozyme and are missing the ALDH2 isozyme. We previou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshida A,Huang IY,Ikawa M

更新日期：1984-01-01 00:00:00

abstract：:We have developed a color complementation assay that allows rapid screening of specific genomic DNA sequences. It is based on the simultaneous amplification of two or more DNA segments with fluorescent oligonucleotide primers such that the generation of a color, or combination of colors, can be visualized and used for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chehab FF,Kan YW

更新日期：1989-12-01 00:00:00

abstract：:Noonan syndrome (NS) is a relatively common genetic disorder, characterized by typical facies, short stature, developmental delay, and cardiac abnormalities. Known causative genes account for 70-80% of clinically diagnosed NS patients, but the genetic basis for the remaining 20-30% of cases is unknown. We performed ne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen PC,Yin J,Yu HW,Yuan T,Fernandez M,Yung CK,Trinh QM,Peltekova VD,Reid JG,Tworog-Dube E,Morgan MB,Muzny DM,Stein L,McPherson JD,Roberts AE,Gibbs RA,Neel BG,Kucherlapati R

更新日期：2014-08-05 00:00:00

abstract：:Two affinity species of the interleukin 2 (IL-2) receptor are different states of a single receptor molecule. We assumed that a binary complex between the IL-2 receptor and another lymphocyte-specific protein would constitute the high-affinity receptor. To test this assumption, we counted the numbers of IL-2 receptors...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo S,Shimizu A,Saito Y,Kinoshita M,Honjo T

更新日期：1986-12-01 00:00:00

abstract：:Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dasgupta S,Mitra S

更新日期：1978-01-01 00:00:00

abstract：:The distribution of dopamine-containing processes in the striatum of fetal and neonatal cats was studied by immunohistochemical and glyoxylic acid histofluorescence methods and compared to the distribution of acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) observed by thiocholine histochemistry in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graybiel AM,Pickel VM,Joh TH,Reis DJ,Ragsdale CW Jr

更新日期：1981-09-01 00:00:00

abstract：:Lipid rafts are important signaling platforms in T cells. Little is known about their properties in human CD8(+) T cells. We studied polarization of lipid rafts by digital immunofluorescence microscopy in primary human T cells, using beads coated with anti-CD3 and anti-CD28 mAbs (CD3/28 beads). Unlike CD4(+) T cells, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovacs B,Maus MV,Riley JL,Derimanov GS,Koretzky GA,June CH,Finkel TH

更新日期：2002-11-12 00:00:00

abstract：:To investigate the contribution of interleukin-4 (IL-4) to airway inflammation in vivo and to explore directly its relationship to airway reactivity, we created transgenic mice in which the murine cDNA for IL-4 was regulated by the rat Clara cell 10 protein promoter. Expression was detected only in the lung and not in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rankin JA,Picarella DE,Geba GP,Temann UA,Prasad B,DiCosmo B,Tarallo A,Stripp B,Whitsett J,Flavell RA

更新日期：1996-07-23 00:00:00

abstract：:Telomerase is a special reverse transcriptase that extends one strand of the telomere repeat by using a template embedded in an RNA subunit. Like other polymerases, telomerase is believed to use a pair of divalent metal ions (coordinated by a triad of aspartic acid residues) for catalyzing nucleotide addition. Here we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lue NF,Bosoy D,Moriarty TJ,Autexier C,Altman B,Leng S

更新日期：2005-07-12 00:00:00

abstract：:Symbiotic microbes impact the severity of a variety of diseases through regulation of T-cell development. However, little is known regarding the molecular mechanisms by which this is accomplished. Here we report that a secreted factor, Erdr1, is regulated by the microbiota to control T-cell apoptosis. Erdr1 expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soto R,Petersen C,Novis CL,Kubinak JL,Bell R,Stephens WZ,Lane TE,Fujinami RS,Bosque A,O'Connell RM,Round JL

更新日期：2017-05-23 00:00:00

abstract：:Using direct percussion, language-competent bonobo-chimpanzees Kanzi and Pan-Banisha produced a significantly wider variety of flint tool types than hitherto reported, and used them task-specifically to break wooden logs or to dig underground for food retrieval. For log breaking, small flakes were rotated drill-like o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roffman I,Savage-Rumbaugh S,Rubert-Pugh E,Ronen A,Nevo E

更新日期：2012-09-04 00:00:00

abstract：:The cytochrome bc1 and related complexes are essential energy-conserving components of mitochondrial and bacterial electron transport chains. They orchestrate a complex sequence of electron and proton transfer reactions resulting in the oxidation of quinol, the reduction of a mobile electron carrier, and the transloca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cape JL,Bowman MK,Kramer DM

更新日期：2007-05-08 00:00:00

abstract：:Molecular clocks have been used to date the divergence of humans and chimpanzees for nearly four decades. Nonetheless, this date and its confidence interval remain to be firmly established. In an effort to generate a genomic view of the human-chimpanzee divergence, we have analyzed 167 nuclear protein-coding genes and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar S,Filipski A,Swarna V,Walker A,Hedges SB

更新日期：2005-12-27 00:00:00

abstract：:Although recent studies have shown that brown adipose tissue (BAT) arises from progenitor cells that also give rise to skeletal muscle, the developmental signals that control the formation of BAT remain largely unknown. Here, we show that brown preadipocytes possess primary cilia and can respond to Hedgehog (Hh) signa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nosavanh L,Yu DH,Jaehnig EJ,Tong Q,Shen L,Chen MH

更新日期：2015-04-21 00:00:00

abstract：:The activation of additional promoter sites by production of an alternative sigma subunit for RNA polymerase is a common strategy for the coordinate regulation of gene expression. Many alternative sigma factors control genes for specialized, and often narrowly distributed, functions. For example, most of the alternati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YF,Helmann JD

更新日期：1992-06-01 00:00:00

abstract：:Global loss of DNA methylation has been known for decades as an epigenomic aberration associated with carcinogenesis and cancer progression. Loss of DNA methylation affects predominantly repetitive elements, which encompass >50% of the CpG dinucleotides present in the human genome. Because of the lack of an effective ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie H,Wang M,Bonaldo Mde F,Rajaram V,Stellpflug W,Smith C,Arndt K,Goldman S,Tomita T,Soares MB

更新日期：2010-04-13 00:00:00

abstract：:Aquaporin (AQP) 4 is the predominant water channel in the mammalian brain, abundantly expressed in the blood-brain and brain-cerebrospinal fluid interfaces of glial cells. Its function in cerebral water balance has implications in neuropathological disorders, including brain edema, stroke, and head injuries. The 1.8-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho JD,Yeh R,Sandstrom A,Chorny I,Harries WE,Robbins RA,Miercke LJ,Stroud RM

更新日期：2009-05-05 00:00:00

abstract：:Presented analysis of human and fly life tables proves that with the specified accuracy their entire survival and mortality curves are uniquely determined by a single point (e.g., by the birth mortality q(0)), according to the law, which is universal for species as remote as humans and flies. Mortality at any age decr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Azbel' MY

更新日期：1999-12-21 00:00:00

abstract：:Interleukin (IL)-15 and its specific receptor chain, IL-15Rα, support the development of various effector cells, including NK and CD8 T cells via a mechanism called trans-presentation. Whereas the dynamic of trans-presentation has been shown to involve the recycling of IL-15Rα by presenting cells, the way responding c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tamzalit F,Barbieux I,Plet A,Heim J,Nedellec S,Morisseau S,Jacques Y,Mortier E

更新日期：2014-06-10 00:00:00

abstract：:Changes in the expression of Hox genes have been widely linked to the evolution of animal body plans, but functional demonstrations of this relationship have been impeded by the lack of suitable model organisms. A classic case study involves the repeated evolution of specialized feeding appendages, called maxillipeds,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pavlopoulos A,Kontarakis Z,Liubicich DM,Serano JM,Akam M,Patel NH,Averof M

更新日期：2009-08-18 00:00:00