当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > The replisome organizer (G38P) of Bacillus subtilis bacteriophage SPP1 forms specialized nucleoprotein complexes with two discrete distant regions of the SPP1 genome.

The replisome organizer (G38P) of Bacillus subtilis bacteriophage SPP1 forms specialized nucleoprotein complexes with two discrete distant regions of the SPP1 genome.

Abstract：

:Initiation of Bacillus subtilis bacteriophage SPP1 DNA replication requires the products of genes 38, 39 and 40 (G38P, G39P and G40P). G38P specifically binds two discrete regions, which are 32.1 kb apart in a linear map of the SPP1 genome. One of these target sites, which maps at the left end of the phage genome, within gene 38, was shown to function as an origin of replication and was therefore termed left origin (oriL). The other site, which lies within a non-coding segment in the late transcribed region on the right end of the genome, was termed oriR. Both sites contain two types of repeated elements (termed Box AB and A + T-rich region). The K(app) for the G38P-oriL DNA and G38P-oriR DNA complexes was estimated to be 1 nM and 4 nM, respectively. G38P binds to the distant oriL and oriR sites cooperatively. DNase I footprinting experiments showed protection by G38P in Box AB, but not in the A + T-rich region. Electron microscopy analysis showed that G38P forms a higher-order nucleoprotein structure with the SPP1 oriL and oriR sites through protein-protein interaction. G38P binding at its cognate sites does not seem to modify the length of the DNA, but to bend it. These results suggest that G38P forms a nucleoprotein complex on the regions where the SPP1 replication origins were previously predicted.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Missich R,Weise F,Chai S,Lurz R,Pedré X,Alonso JC

doi

10.1006/jmbi.1997.1060

subject

Has Abstract

pub_date

1997-07-04 00:00:00

pages

50-64

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91060-3

journal_volume

270

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Expression and transmission of a rodent retrovirus-like (VL30) gene family.

    abstract：:A transcriptionally active sub-set of the dispersed mouse VL30 family of proviral genetic elements was shown to be highly transmissible as a murine leukaemia virus pseudotype. Newly acquired VL30 proviruses (present at 1 to 2 copies per cell) were shown to be transcriptionally active. These data substantiate the hypot...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90485-7

    authors： Carter AT,Norton JD,Gibson Y,Avery RJ

    更新日期：1986-03-05 00:00:00

  • Molecular structure of a novel membrane protease specific for a stomatin homolog from the hyperthermophilic archaeon Pyrococcus horikoshii.

    abstract：:Membrane-bound proteases are involved in various regulatory functions. Our previous study indicated that the N-terminal region of an open reading frame, PH1510 (residues 16-236, designated as 1510-N) from the hyperthermophilic archaeon Pyrococcus horikoshii, is a serine protease with a catalytic Ser-Lys dyad that spec...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.02.052

    authors： Yokoyama H,Matsui E,Akiba T,Harata K,Matsui I

    更新日期：2006-05-12 00:00:00

  • Structural analysis of the rate-limiting transition states in the folding of Im7 and Im9: similarities and differences in the folding of homologous proteins.

    abstract：:The bacterial immunity proteins Im7 and Im9 fold with mechanisms of different kinetic complexity. Whilst Im9 folds in a two-state transition at pH 7.0 and 10 degrees C, Im7 populates an on-pathway intermediate under these conditions. In order to assess the role of sequence versus topology in the folding of these prote...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01249-4

    authors： Friel CT,Capaldi AP,Radford SE

    更新日期：2003-02-07 00:00:00

  • Position-specific inhibition of yeast mitochondrial transcription by a poly(T) sequence.

    abstract：:The 3' flanking nucleotide(s) of the octanucleotide promoter sequence regulates transcriptional efficiency of some mitochondrial genes in Saccharomyces cerevisiae. To understand this regulation the in vitro transcriptional activity of various synthetic mitochondrial promoters carrying different 3' flanking sequences w...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1483

    authors： Biswas TK,Getz GS

    更新日期：1998-01-30 00:00:00

  • The crystal structure at 2A resolution of the Ca2+ -binding protein S100P.

    abstract：:S100P is a small calcium-binding protein of the S100 EF-hand-containing family of proteins. Elevated levels of its mRNA are reported to be associated with the progression to hormone independence and metastasis of prostate cancer and to be associated with loss of senescence in human breast epithelial cells in vitro. Th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01278-0

    authors： Zhang H,Wang G,Ding Y,Wang Z,Barraclough R,Rudland PS,Fernig DG,Rao Z

    更新日期：2003-01-24 00:00:00

  • Structural studies of the final enzyme in the alpha-aminoadipate pathway-saccharopine dehydrogenase from Saccharomyces cerevisiae.

    abstract：:The 1.64 A structure of the apoenzyme form of saccharopine dehydrogenase (SDH) from Saccharomyces cerevisiae shows the enzyme to be composed of two domains with similar dinucleotide binding folds with a deep cleft at the interface. The structure reveals homology to alanine dehydrogenase, despite low primary sequence s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.08.044

    authors： Burk DL,Hwang J,Kwok E,Marrone L,Goodfellow V,Dmitrienko GI,Berghuis AM

    更新日期：2007-10-26 00:00:00

  • Probing the structural domains and function in vivo of Escherichia coli DNA topoisomerase I by mutagenesis.

    abstract：:Insertion and deletion mutagenesis within the gene topA of Escherichia coli encoding DNA topoisomerase I was carried out to test the existence of subdomains in the enzyme and the relationship between the slow-growth topA- phenotype and the known DNA relaxation activity of the enzyme. All mutants that show no detectabl...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90130-0

    authors： Zumstein L,Wang JC

    更新日期：1986-10-05 00:00:00

  • cAMP-dependent protein kinase A selects the excited state of the membrane substrate phospholamban.

    abstract：:Phosphorylation of membrane proteins is a central regulatory and signaling mechanism across cell compartments. However, the recognition process and phosphorylation mechanism of membrane-bound substrates by kinases are virtually unknown. cAMP-dependent protein kinase A (PKA) is a ubiquitous enzyme that phosphorylates s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.06.041

    authors： Masterson LR,Yu T,Shi L,Wang Y,Gustavsson M,Mueller MM,Veglia G

    更新日期：2011-09-16 00:00:00

  • beta-hairpin stability and folding: molecular dynamics studies of the first beta-hairpin of tendamistat.

    abstract：:The stability and (un)folding of the 19-residue peptide, SCVTLYQSWRYSQADNGCA, corresponding to the first beta-hairpin (residues 10 to 28) of the alpha-amylase inhibitor tendamistat (PDB entry 3AIT) has been studied by molecular dynamics simulations in explicit water under periodic boundary conditions at several temper...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3446

    authors： Bonvin AM,van Gunsteren WF

    更新日期：2000-02-11 00:00:00

  • Identification of thermodynamically relevant interactions between EF-Tu and backbone elements of tRNA.

    abstract：:A set of 45 different tRNAs, each containing a single deoxynucleotide substitution covering the upper half of the molecule was used in conjunction with a high-throughput ribonuclease protection assay to investigate the thermodynamic role of 2' hydroxyl groups in stabilizing a complex with elongation factor Tu (EF-Tu) ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4612

    authors： Pleiss JA,Uhlenbeck OC

    更新日期：2001-05-18 00:00:00

  • Human T cell cyclophilin18 binds to thiol-specific antioxidant protein Aop1 and stimulates its activity.

    abstract：:Cyclophilins (CyPs) define a family of proteins binding to the immunosuppressive drug cyclosporin A (CsA). They are evolutionary highly conserved proteins being present in both pro- and eukaryotes and in different subcellular locations. CyPs possess enzymatic activity, namely peptidyl-prolyl cis-trans isomerase (PPIas...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1644

    authors： Jäschke A,Mi H,Tropschug M

    更新日期：1998-04-10 00:00:00

  • Developmental regulation of the aldolase A muscle-specific promoter during in vivo muscle maturation is controlled by a nuclear receptor binding element.

    abstract：:During the post-natal period, skeletal muscles undergo important modifications leading to the appearance of different types of myofibers which exhibit distinct contractile and metabolic properties. This maturation process results from the activation of the expression of different sets of contractile proteins and metab...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2821

    authors： Spitz F,Demignon J,Kahn A,Daegelen D,Maire P

    更新日期：1999-06-18 00:00:00

  • An algorithm for the prediction of proteasomal cleavages.

    abstract：:Proteasomes, major proteolytic sites in eukaryotic cells, play an important part in major histocompatibility class I (MHC I) ligand generation and thus in the regulation of specific immune responses. Their cleavage specificity is of outstanding interest for this process. In order to generalize previously determined cl...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3683

    authors： Kuttler C,Nussbaum AK,Dick TP,Rammensee HG,Schild H,Hadeler KP

    更新日期：2000-05-05 00:00:00

  • Capsids and Portals Influence Each Other's Conformation During Assembly and Maturation.

    abstract：:The portal proteins of tailed bacteriophage and Herpesvirus capsids form dodecameric rings that occupy one capsid vertex and are incorporated during the assembly of capsid precursors called procapsids or proheads. Portals are essential and serve as the pore for DNA transit and the site of tail attachment; however, bac...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.01.022

    authors： Maurer JB,Oh B,Moyer CL,Duda RL

    更新日期：2020-03-27 00:00:00

  • Isoform-selective interaction of the adaptor protein Tks5/FISH with Sos1 and dynamins.

    abstract：:The adaptor protein Tks5/FISH (tyrosine kinase substrate 5/five SH3 domains, hereafter termed Tks5) is a crucial component of a protein network that controls the invasiveness of cancer cells and progression of Alzheimer's disease. Tks5 consists of an amino-terminal PX domain that is followed by five SH3 domains (SH3A-...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.05.025

    authors： Rufer AC,Rumpf J,von Holleben M,Beer S,Rittinger K,Groemping Y

    更新日期：2009-07-31 00:00:00

  • Phosphorylation driven motions in the COOH-terminal Src kinase, CSK, revealed through enhanced hydrogen-deuterium exchange and mass spectrometry (DXMS).

    abstract：:Previous kinetic studies demonstrated that nucleotide-derived conformational changes regulate function in the COOH-terminal Src kinase. We have employed enhanced methods of hydrogen-deuterium exchange-mass spectrometry (DXMS) to probe conformational changes on CSK in the absence and presence of nucleotides and thereby...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01003-3

    authors： Hamuro Y,Wong L,Shaffer J,Kim JS,Stranz DD,Jennings PA,Woods VL Jr,Adams JA

    更新日期：2002-11-08 00:00:00

  • The structure of Sif2p, a WD repeat protein functioning in the SET3 corepressor complex.

    abstract：:In Saccharomyces cerevisiae, the SIF2 gene product is an integral component of the Set3 complex (SET3C), an assembly of proteins with some homology to the human SMRT and N-CoR corepressor complexes. SET3C has histone deacetylase activity that is responsible for repressing a set of meiotic genes. We have determined the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.06.025

    authors： Cerna D,Wilson DK

    更新日期：2005-08-26 00:00:00

  • pH-dependent interactions and the stability and folding kinetics of the N-terminal domain of L9. Electrostatic interactions are only weakly formed in the transition state for folding.

    abstract：:The role of electrostatic interactions in the stability and the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) was investigated by determining the effects of varying the pH conditions. Urea denaturations and thermal unfolding experiments were used to measure the free energy of folding, DeltaG degr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3752

    authors： Luisi DL,Raleigh DP

    更新日期：2000-06-16 00:00:00

  • Crystal structure of human ribosomal protein L10 core domain reveals eukaryote-specific motifs in addition to the conserved fold.

    abstract：:A phylogenetically conserved ribosomal protein L16p/L10e organizes the architecture of the aminoacyl tRNA binding site on the large ribosomal subunit. Eukaryotic L10 also exhibits a variety of cellular activities, and, in particular, human L10 is known as a putative tumor suppressor, QM. We have determined the 2.5-A c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.01.003

    authors： Nishimura M,Kaminishi T,Takemoto C,Kawazoe M,Yoshida T,Tanaka A,Sugano S,Shirouzu M,Ohkubo T,Yokoyama S,Kobayashi Y

    更新日期：2008-03-21 00:00:00

  • Interaction of Alzheimer's A beta peptide with an engineered binding protein--thermodynamics and kinetics of coupled folding-binding.

    abstract：:The oligomerization and aggregation of the amyloid-beta (A beta) peptide, a cleavage product of the amyloid precursor protein predominantly 40 or 42 amino acids in length, has been implicated in the pathogenesis of Alzheimer's disease. The identification of A beta-binding agents, e.g., antibodies or peptides, constitu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.02.040

    authors： Hoyer W,Härd T

    更新日期：2008-04-25 00:00:00

  • Molecular insights into the interaction between alpha-synuclein and docosahexaenoic acid.

    abstract：:alpha-Synuclein (alpha-syn) is a 140-residue protein of unknown function, involved in several neurodegenerative disorders, such as Parkinson's disease. Recently, the possible interaction between alpha-syn and polyunsaturated fatty acids has attracted a strong interest. Indeed, lipids are able to trigger the multimeriz...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.09.008

    authors： De Franceschi G,Frare E,Bubacco L,Mammi S,Fontana A,de Laureto PP

    更新日期：2009-11-20 00:00:00

  • Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein.

    abstract：:Fasciculin 2 and toxin alpha proteins belong to the same structural family of three-fingered snake toxins. They act on different targets, but in each case the binding region involves residues from loops I and II. The superimposition of the two structures suggests that these functional regions correspond to structurall...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3510

    authors： Le Du MH,Ricciardi A,Khayati M,Ménez R,Boulain JC,Ménez A,Ducancel F

    更新日期：2000-03-03 00:00:00

  • Granulysin crystal structure and a structure-derived lytic mechanism.

    abstract：:Our crystal structure of granulysin suggests a mechanism for lysis of bacterial membranes by granulysin, a 74-residue basic protein from human cytolytic T lymphocyte and natural killer cells. We determined the initial crystal structure of selenomethionyl granulysin by MAD phasing at 2A resolution. We present the struc...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01234-2

    authors： Anderson DH,Sawaya MR,Cascio D,Ernst W,Modlin R,Krensky A,Eisenberg D

    更新日期：2003-01-10 00:00:00

  • Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft.

    abstract：:Penicillin G acylase is a periplasmic protein, cytoplasmically expressed as a precursor polypeptide comprising a signal sequence, the A and B chains of the mature enzyme (209 and 557 residues respectively) joined by a spacer peptide of 54 amino acid residues. The wild-type AB heterodimer is produced by proteolytic rem...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4105

    authors： Hewitt L,Kasche V,Lummer K,Lewis RJ,Murshudov GN,Verma CS,Dodson GG,Wilson KS

    更新日期：2000-09-29 00:00:00

  • Limitation of ribosomal protein L11 availability in vivo affects translation termination.

    abstract：:Historically referred to as "the GTPase center", the L11 binding region (L11BR) of Escherichia coli 23 S rRNA is a highly conserved structure that has been implicated in several essential functions during protein synthesis. Here, in vivo expression of an RNA fragment containing that structure was found to affect trans...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00304-2

    authors： Van Dyke N,Xu W,Murgola EJ

    更新日期：2002-05-31 00:00:00

  • A symmetrical model for the domain structure of type I DNA methyltransferases.

    abstract：:Type I DNA methyltransferases are complex multisubunit enzymes that methylate a specific base in each half of an asymmetric bipartite DNA recognition sequence. The specificity (S) subunit contains two corresponding DNA sequence recognition domains, plus a number of conserved regions which interact with two modificatio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1006/jmbi.1994.1624

    authors： Kneale GG

    更新日期：1994-10-14 00:00:00

  • Crystal structure of the catalytic subunit of Cdc25B required for G2/M phase transition of the cell cycle.

    abstract：:Cdc25B is a dual specificity phosphatase involved in the control of cyclin-dependent kinases and the progression of cells through the cell cycle. A series of minimal domain Cdc25B constructs maintaining catalytic activity have been expressed. The structure of a minimum domain construct binding sulfate was determined a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3168

    authors： Reynolds RA,Yem AW,Wolfe CL,Deibel MR Jr,Chidester CG,Watenpaugh KD

    更新日期：1999-10-29 00:00:00

  • Structure-function analysis of the acyl carrier protein synthase (AcpS) from Mycobacterium tuberculosis.

    abstract：:We have solved the crystal structure of the acyl carrier protein synthase (AcpS) from Mycobacterium tuberculosis (Mtb) at 1.95 A resolution. AcpS, a 4-phosphopantetheinyl transferase, activates two distinct acyl carrier proteins (ACPs) that are present in fatty acid synthase (FAS) systems FAS-I and FAS-II, the ACP-I d...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.08.065

    authors： Dym O,Albeck S,Peleg Y,Schwarz A,Shakked Z,Burstein Y,Zimhony O

    更新日期：2009-11-06 00:00:00

  • Conformational flexibility and polymerization of vesicular stomatitis virus matrix protein.

    abstract：:The matrix protein of vesicular stomatitis virus (VSV) plays a pivotal role in viral assembly. We previously demonstrated the ability of M protein to self-associate at low salt concentrations. Now, we show the ability of M protein to polymerize in the presence of ZnCl2 in a nucleation-dependent manner. Analysis of kin...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1439

    authors： Gaudin Y,Sturgis J,Doumith M,Barge A,Robert B,Ruigrok RW

    更新日期：1997-12-19 00:00:00

  • Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA.

    abstract：:Escherichia coli ribosomal RNA contains five guanosine residues methylated at N2. The ygjO gene was previously predicted to methylate 16 S rRNA residue G966 due to its high sequence homology with the protein RsmC, responsible for G1207 methylation. We have identified the target of YgjO as being m2G1835 of the 23 S rRN...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.09.008

    authors： Sergiev PV,Lesnyak DV,Bogdanov AA,Dontsova OA

    更新日期：2006-11-17 00:00:00