当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA.

Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA.

Abstract：

:Escherichia coli ribosomal RNA contains five guanosine residues methylated at N2. The ygjO gene was previously predicted to methylate 16 S rRNA residue G966 due to its high sequence homology with the protein RsmC, responsible for G1207 methylation. We have identified the target of YgjO as being m2G1835 of the 23 S rRNA and not m2G966 of the 16 S rRNA as expected. Knock-out of the ygjO gene leads to loss of modification at G1835, as revealed by reverse transcription. Moreover, the modification could be restored by in vivo complementation of the ygjO knock-out strain with a plasmid expressing ygjO. Recombinant YgjO protein is able to methylate in vitro protein-free 23 S rRNA, but not assembled 50 S subunits purified from the ygjO knock-out strain. The nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2. Growth competition assays reveal that the lack of the G1835 methylation causes growth retardation, especially at temperatures higher than optimal and in poor media. Based on these results we suggest that YgjO be renamed to RlmG in accordance with the accepted nomenclature for rRNA methyltransferases.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Sergiev PV,Lesnyak DV,Bogdanov AA,Dontsova OA

doi

10.1016/j.jmb.2006.09.008

subject

Has Abstract

pub_date

2006-11-17 00:00:00

pages

26-31

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)01181-8

journal_volume

364

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Folding of the Tetrahymena ribozyme by polyamines: importance of counterion valence and size.

    abstract：:Polyamines are abundant metabolites that directly influence gene expression. Although the role of polyamines in DNA condensation is well known, their role in RNA folding is less understood. Non-denaturing gel electrophoresis was used to monitor the equilibrium folding transitions of the Tetrahymena ribozyme in the pre...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.06.008

    authors： Koculi E,Lee NK,Thirumalai D,Woodson SA

    更新日期：2004-07-30 00:00:00

  • The capsid size-determining protein Sid forms an external scaffold on phage P4 procapsids.

    abstract：:Although the phages P2 and P4 build their capsids from the same precursor, the product of the P2 N gene, the two capsids differ in size: P2 builds a 60 nm, T = 7 capsid from 420 subunits, whereas P4 makes a 45 nm, T = 4 capsid from 240 subunits. This difference leads to substantial changes in shell geometry and subuni...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0416

    authors： Marvik OJ,Dokland T,Nøkling RH,Jacobsen E,Larsen T,Lindqvist BH

    更新日期：1995-08-04 00:00:00

  • Mechanochemistry of a viral DNA packaging motor.

    abstract：:The pentameric ATPase motor gp16 packages double-stranded DNA into the bacteriophage phi29 virus capsid. On the basis of the results of single-molecule experimental studies, we propose a push and roll mechanism to explain how the packaging motor translocates the DNA in bursts of four 2.5 bp power strokes, while rotati...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.05.002

    authors： Yu J,Moffitt J,Hetherington CL,Bustamante C,Oster G

    更新日期：2010-07-09 00:00:00

  • An irregular beta-bulge common to a group of bacterial RNases is an important determinant of stability and function in barnase.

    abstract：:Single amino acid residue substitutions rarely destroy the structural integrity of proteins. Substitution of glycine residues, however, is among the few sorts of alterations that can have such an effect. Here, we seek to understand what accounts for the extreme functional impairment of the bacterial ribonuclease barna...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2569

    authors： Axe DD,Foster NW,Fersht AR

    更新日期：1999-03-12 00:00:00

  • Reconstituted and native iron-cores of bacterioferritin and ferritin.

    abstract：:The structural and magnetic properties of the iron-cores of reconstituted horse spleen ferritin and Azotobacter vinelandii bacterioferritin have been investigated by high-resolution transmission electron microscopy, electron diffraction and Mossbauer spectroscopy. The structural properties of native horse spleen ferri...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90290-7

    authors： Mann S,Williams JM,Treffry A,Harrison PM

    更新日期：1987-12-05 00:00:00

  • Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli.

    abstract：:Two-component regulatory systems allow cells to adapt to environmental changes. In Escherichia coli, the TorS/TorR two-component system induces the expression of the tor structural operon encoding the trimethylamine N-oxide reductase respiratory system in response to substrate availability. TorS belongs to a sensor su...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.0919

    authors： Jourlin C,Ansaldi M,Méjean V

    更新日期：1997-04-11 00:00:00

  • Transition between different binding modes in rat DNA polymerase beta-ssDNA complexes.

    abstract：:Interactions of rat DNA polymerase beta with a single-stranded (ss) DNA have been studied using the quantitative fluorescence titration technique. Examination of the fluorescence changes accompanying the binding, as a function of the thermodynamically rigorous binding density of rat pol beta-ssDNA complexes, reveals t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2252

    authors： Jezewska MJ,Rajendran S,Bujalowski W

    更新日期：1998-12-11 00:00:00

  • Local interactions dominate folding in a simple protein model.

    abstract：:Recent computational studies of simple models of protein folding have concluded that a pronounced energy minimum (i.e. large gap in energy between low-energy states of the model) is a necessary and sufficient condition to ensure folding of a sequence to its lowest-energy conformation. Here, we show that this conclusio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0375

    authors： Unger R,Moult J

    更新日期：1996-06-28 00:00:00

  • Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.

    abstract：:The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show si...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.06.006

    authors： Garcia-Pino A,Buts L,Wyns L,Loris R

    更新日期：2006-08-04 00:00:00

  • Force-induced conformational change of bacteriorhodopsin.

    abstract：:The cytoplasmic surface topography of purple membranes imaged by the atomic force microscope depends mainly on the force applied to the stylus. Imaged at forces of 300 pN, individual bacteriorhodopsin molecules reveal two domains. The resulting donut-shaped trimers reversibly transform into structures exhibiting three...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0292

    authors： Müller DJ,Büldt G,Engel A

    更新日期：1995-06-02 00:00:00

  • Cell Heterogeneity in Staphylococcal Communities.

    abstract：:The human pathogen Staphylococcus aureus is a gram-positive bacterium that causes difficult-to-treat infections. One of the reasons why S. aureus is such as successful pathogen is due to the cell-to-cell physiological variability that exists within microbial communities. Many laboratories around the world study the ge...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2019.06.011

    authors： García-Betancur JC,Lopez D

    更新日期：2019-11-22 00:00:00

  • The structure of the cytochrome p450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography.

    abstract：:Cytochrome P450cam catalyzes the hydroxylation of camphor in a complex process involving two electron transfers (ETs) from the iron-sulfur protein putidaredoxin. The enzymatic control of the successive steps of catalysis is critical for a highly efficient reaction. The injection of the successive electrons is part of ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.07.006

    authors： Hiruma Y,Hass MA,Kikui Y,Liu WM,Ölmez B,Skinner SP,Blok A,Kloosterman A,Koteishi H,Löhr F,Schwalbe H,Nojiri M,Ubbink M

    更新日期：2013-11-15 00:00:00

  • Native and non-native structure in a protein-folding intermediate: spectroscopic studies of partially reduced IGF-I and an engineered alanine model.

    abstract：:The structure of a metastable folding intermediate of human insulin-like growth factor I (IGF-I) and an engineered model are investigated by circular dichroism and two-dimensional 1H NMR spectroscopy. The intermediate, which contains two of three native disulfide bonds, was trapped by acid quenching and isolated by re...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0320

    authors： Hua QX,Narhi L,Jia W,Arakawa T,Rosenfeld R,Hawkins N,Miller JA,Weiss MA

    更新日期：1996-06-07 00:00:00

  • The Molecular Basis of MeCP2 Function in the Brain.

    abstract：:MeCP2 is a reader of the DNA methylome that occupies a large proportion of the genome due to its high abundance and the frequency of its target sites. It has been the subject of extensive study because of its link with 'MECP2-related disorders', of which Rett syndrome is the most prevalent. This review integrates evid...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2019.10.004

    authors： Tillotson R,Bird A

    更新日期：2019-10-17 00:00:00

  • Topological and energetic factors: what determines the structural details of the transition state ensemble and "en-route" intermediates for protein folding? An investigation for small globular proteins.

    abstract：:Recent experimental results suggest that the native fold, or topology, plays a primary role in determining the structure of the transition state ensemble, at least for small, fast-folding proteins. To investigate the extent of the topological control of the folding process, we studied the folding of simplified models ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3693

    authors： Clementi C,Nymeyer H,Onuchic JN

    更新日期：2000-05-19 00:00:00

  • Human cardiac myosin binding protein C: structural flexibility within an extended modular architecture.

    abstract：:New insights into the modular organization and flexibility of the N-terminal half of human cardiac myosin binding protein C (cMyBP-C) and information on the association state of the full-length protein have been deduced from a combined small-angle X-ray scattering (SAXS) and NMR study. SAXS data show that the first fi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.10.029

    authors： Jeffries CM,Lu Y,Hynson RM,Taylor JE,Ballesteros M,Kwan AH,Trewhella J

    更新日期：2011-12-16 00:00:00

  • Structure and function of X-Pro dipeptide repeats in the TonB proteins of Salmonella typhimurium and Escherichia coli.

    abstract：:The TonB protein is required for several outer membrane transport processes in bacteria. A short 33-residue peptide segment of TonB has been studied by 1H and 13C nuclear magnetic resonance spectroscopy. The sequence of this peptide segment contains multiple Glu-Pro and Lys-Pro dipeptide repeats that maintain rigid, e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(99)80008-4

    authors： Brewer S,Tolley M,Trayer IP,Barr GC,Dorman CJ,Hannavy K,Higgins CF,Evans JS,Levine BA,Wormald MR

    更新日期：1990-12-20 00:00:00

  • Peroxisome proliferator-activated receptor alpha controls hepatic heme biosynthesis through ALAS1.

    abstract：:Heme is an essential prosthetic group of proteins involved in oxygen transport, energy metabolism and nitric oxide production. ALAS1 (5-aminolevulinate synthase) is the rate-limiting enzyme in heme synthesis in the liver and is highly regulated to adapt to the metabolic demand of the hepatocyte. In the present study, ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.jmb.2009.03.024

    authors： Degenhardt T,Väisänen S,Rakhshandehroo M,Kersten S,Carlberg C

    更新日期：2009-05-01 00:00:00

  • Cubic phases of lipid-containing systems. A translational diffusion study by fluorescence recovery after photobleaching.

    abstract：:The lateral diffusion coefficient of fluorescent lipid analogues incorporated in four cubic phases of lipid-water systems was determined by the modulated fringe pattern photobleaching technique. In two of the phases, Q230 and Q224, whose structure is bicontinuous, the diffusion is almost as fast as in the fluid lipid ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1051

    authors： Cribier S,Gulik A,Fellmann P,Vargas R,Devaux PF,Luzzati V

    更新日期：1993-01-20 00:00:00

  • Structure of complexes between a major protein of heterogeneous nuclear ribonucleoprotein particles and polyribonucleotides.

    abstract：:We have investigated the structure of complexes formed between a series of poly(A)n (n = 30 to 480) and HD40 (helix-destabilizing protein, molecular weight of 40,000), the major protein component of 30 S heterogeneous nuclear ribonucleoprotein particles (hnRNP) from the brine shrimp Artemia salina. Protein HD40 is sim...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(83)90039-6

    authors： Thomas JO,Glowacka SK,Szer W

    更新日期：1983-12-25 00:00:00

  • Crystal structure of the agrin-responsive immunoglobulin-like domains 1 and 2 of the receptor tyrosine kinase MuSK.

    abstract：:Muscle-specific kinase (MuSK) is a receptor tyrosine kinase expressed exclusively in skeletal muscle, where it is required for formation of the neuromuscular junction. MuSK is activated by agrin, a neuron-derived heparan sulfate proteoglycan. Here, we report the crystal structure of the agrin-responsive first and seco...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.09.019

    authors： Stiegler AL,Burden SJ,Hubbard SR

    更新日期：2006-12-01 00:00:00

  • Molecular interactions and residues involved in force generation in the T4 viral DNA packaging motor.

    abstract：:Many viruses utilize molecular motors to package their genomes into preformed capsids. A striking feature of these motors is their ability to generate large forces to drive DNA translocation against entropic, electrostatic, and bending forces resisting DNA confinement. A model based on recently resolved structures of ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2014.09.023

    authors： Migliori AD,Smith DE,Arya G

    更新日期：2014-12-12 00:00:00

  • A cis-prolyl peptide bond isomerization dominates the folding of the alpha subunit of Trp synthase, a TIM barrel protein.

    abstract：:The cis/trans isomerization of prolyl peptide bonds has been suggested to dominate the folding of the alpha subunit of tryptophan synthase from Escherichia coli (alphaTS). To test the role of the unique cis isomer between Asp27 and Pro28, the folding properties of P28A, P28G and G(3)P28G, a three-glycine insertion mut...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00737-4

    authors： Wu Y,Matthews CR

    更新日期：2002-09-06 00:00:00

  • Reverse turns in blocked dipeptides are intrinsically unstable in water.

    abstract：:We have carried out molecular dynamics simulations to study the conformational equilibria of two blocked dipeptides, Ac-Ala-Ala-NHMe and trans-Ac-Pro-Ala-NHMe, in water (Ac, amino-terminal blocking group COCH3; NHMe, carboxy-terminal blocking group NHCH3). Using specialized sampling techniques we computed free-energy ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90399-7

    authors： Tobias DJ,Sneddon SF,Brooks CL 3rd

    更新日期：1990-12-05 00:00:00

  • Crystallization of serine carboxypeptidases.

    abstract：:Crystallization of three different serine carboxypeptidases has been achieved by the method of hanging-drop vapor diffusion. Serine carboxypeptidases II from wheat bran and malted barley crystallize isomorphously from polyethylene glycol solutions at room temperature (pH 4 to 7) in space group P4(1)2(1)2 or enantiomor...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90352-m

    authors： Wilson KP,Liao DI,Bullock T,Remington SJ,Breddam K

    更新日期：1990-01-20 00:00:00

  • Functional role of the "ionic lock"--an interhelical hydrogen-bond network in family A heptahelical receptors.

    abstract：:Activation of family A G-protein-coupled receptors involves a rearrangement of a conserved interhelical cytoplasmic hydrogen bond network between the E(D)RY motif on transmembrane helix 3 (H3) and residues on H6, which is commonly termed the cytoplasmic "ionic lock." Glu134(3.49) of the E(D)RY motif also forms an intr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.05.022

    authors： Vogel R,Mahalingam M,Lüdeke S,Huber T,Siebert F,Sakmar TP

    更新日期：2008-07-18 00:00:00

  • Crystal structure of three consecutive laminin-type epidermal growth factor-like (LE) modules of laminin gamma1 chain harboring the nidogen binding site.

    abstract：:The structure of three consecutive laminin-type EGF-like (LE) modules of mouse laminin gammma1 chain, gamma1III3-5 (positions 738 to 899), has been determined by multiple isomorphous replacement in a crystal of space group p6(4)22 (a=b=74.57 angstroms, c = 185.11 angstroms and gamma = 120 degrees). The crystal structu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0191

    authors： Stetefeld J,Mayer U,Timpl R,Huber R

    更新日期：1996-04-05 00:00:00

  • Crystallization of p68 on lipid monolayers and as three-dimensional single crystals.

    abstract：:Two-dimensional crystals of p68, a Ca2+ -binding protein that has homology with members of the lipocortin/calpactin family, were obtained by interaction with a phospholipid monolayer. By measuring surface pressure at constant surface area, p68 was found to interact in a Ca2+ -dependent manner specifically with phospha...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90534-2

    authors： Newman R,Tucker A,Ferguson C,Tsernoglou D,Leonard K,Crumpton MJ

    更新日期：1989-03-05 00:00:00

  • Local folding coupled to RNA binding in the yeast ribosomal protein L30.

    abstract：:The ribosomal protein L30 from yeast Saccharomyces cerevisiae auto-regulates its own synthesis by binding to a structural element in both its pre-mRNA and its mRNA. The three-dimensional structures of L30 in the free (f L30) and the pre-mRNA bound (b L30) forms have been solved by nuclear magnetic resonance spectrosco...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3044

    authors： Mao H,Williamson JR

    更新日期：1999-09-17 00:00:00

  • Thermodynamic and kinetic characterization of ligand binding to the purine riboswitch aptamer domain.

    abstract：:Riboswitches are cis-acting genetic regulatory elements found commonly in bacterial mRNAs that consist of a metabolite-responsive aptamer domain coupled to a regulatory switch. Purine riboswitches respond to intracellular concentrations of either adenine or guanine/hypoxanthine to control gene expression. The aptamer ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.04.003

    authors： Gilbert SD,Stoddard CD,Wise SJ,Batey RT

    更新日期：2006-06-09 00:00:00