Crystallization of serine carboxypeptidases.

abstract：:The induction of effector genes that encode enzymes is often controlled by the protein product of a regulator gene that is directly involved in the control of its own expression. This coupling of elementary gene circuits can lead to three patterns of regulator and effector gene expression. As effector gene expression ...

journal_title：Journal of molecular biology

authors： Hlavacek WS,Savageau MA

更新日期：1996-01-12 00:00:00

abstract：:Using simple design and selective pressure, we have evolved an artificial M13 bacteriophage coat protein. M13 coat proteins first reside in the bacterial inner membrane and subsequently surround the DNA core of the assembled virus. The artificial coat protein (ACP) was designed and evolved to mimic both functions of t...

journal_title：Journal of molecular biology

authors： Weiss GA,Sidhu SS

更新日期：2000-06-30 00:00:00

abstract：:Interactions of transmembrane helices play an important role in folding and oligomerization of integral membrane proteins. The interfacial residues of these helices frequently correspond to heptad repeat motifs. In order to uncover novel mechanisms underlying these interactions, we randomised a heptad repeat pattern w...

journal_title：Journal of molecular biology

authors： Ridder A,Skupjen P,Unterreitmeier S,Langosch D

更新日期：2005-12-09 00:00:00

abstract：:The region of bacteriophage P1 DNA containing a lytic (vegetative) replicon has been identified by cloning P1 fragments into a phage lambda vector. We present the sequence of that replicon. Using a novel fusion vector containing two P1 loxP recombination sites, we have developed a transformation assay for replicon fun...

journal_title：Journal of molecular biology

authors： Sternberg N,Cohen G

更新日期：1989-05-05 00:00:00

abstract：:Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression. The catalytic domain of this dual-specificity phosphatase has recently been mapped to the 180 most C-terminal amino acids. ...

journal_title：Journal of molecular biology

authors： Hofmann K,Bucher P,Kajava AV

更新日期：1998-09-11 00:00:00

abstract：:Interactions of polymerase X from African swine fever virus with single-stranded DNA (ssDNA) have been studied, using quantitative fluorescence titration and analytical ultracentrifugation techniques. Experiments were performed with a fluorescent etheno-derivative of ssDNA oligomers. Studies of unmodified ssDNA oligom...

journal_title：Journal of molecular biology

authors： Jezewska MJ,Marcinowicz A,Lucius AL,Bujalowski W

更新日期：2006-02-10 00:00:00

abstract：:Alphavirus replicons are very useful for analyzing different aspects of viral molecular biology. They are also useful tools in the development of new vaccines and highly efficient expression of heterologous genes. We have investigated the translatability of Sindbis virus (SV) subgenomic mRNA bearing different 5'-untra...

journal_title：Journal of molecular biology

authors： Sanz MA,Welnowska E,Redondo N,Carrasco L

更新日期：2010-09-10 00:00:00

abstract：:Transcription complexes containing Escherichia coli RNA polymerase terminate RNA synthesis in response to intrinsic (rho-independent) terminators encoded in the DNA template. This type of termination involves the formation of a hairpin structure in the nascent RNA, leading to release of the RNA and polymerase from the...

journal_title：Journal of molecular biology

authors： Wilson KS,von Hippel PH

更新日期：1994-11-18 00:00:00

abstract：:EcoP15I is a Type III restriction endonuclease requiring the interaction with two inversely oriented 5'-CAGCAG recognition sites for efficient DNA cleavage. Diverse models have been developed to explain how enzyme complexes bound to both sites move toward each other, DNA translocation, DNA looping and simple diffusion...

journal_title：Journal of molecular biology

authors： Möncke-Buchner E,Rothenberg M,Reich S,Wagenführ K,Matsumura H,Terauchi R,Krüger DH,Reuter M

更新日期：2009-04-17 00:00:00

abstract：:RusA is a Holliday junction resolvase encoded by the cryptic prophage DLP12 of Escherichia coli K-12 that can be activated to promote homologous recombination and DNA repair in resolution-deficient mutants lacking the RuvABC proteins. Database searches with the 120 amino acid residue RusA sequence identified 11 homolo...

journal_title：Journal of molecular biology

authors： Bolt EL,Sharples GJ,Lloyd RG

更新日期：1999-02-19 00:00:00

abstract：:Single nucleotide changes to the sequence between two alternative 5' splice sites, separated by 25 nucleotides in a beta-globin gene derivative, caused substantial shifts in pre-mRNA splicing preferences, both in vivo and in vitro. An activating sequence for splicing was located. Models for the recognition by U1 small...

journal_title：Journal of molecular biology

authors： Cunningham SA,Else AJ,Potter BV,Eperon IC

更新日期：1991-01-20 00:00:00

abstract：:The structures of the two very closely related proteins, bovine gamma II- and gamma IVa-crystallin have been studied by means of near-ultra-violet linear dichroism spectroscopy on squeezed polyacrylamide gel systems. The crystallin spectra are discussed in terms of the spectra of the aromatic chromophores present in t...

journal_title：Journal of molecular biology

authors： Bloemendal M,Leunissen JA,van Amerongen H,van Grondelle R

更新日期：1990-11-05 00:00:00

abstract：:Fibritin is a fibrous protein that forms "whiskers" attached to the neck of bacteriophage T4. Whiskers interact with the long tail fibers regulating the assembly and infectivity of the virus. The fibritin trimer includes the N-terminal domain responsible for attachment to the phage particle and for the collar formatio...

journal_title：Journal of molecular biology

authors： Boudko SP,Strelkov SV,Engel J,Stetefeld J

更新日期：2004-06-11 00:00:00

abstract：:Current data suggest that globular domains may form amyloids via different mechanisms. Nevertheless, there are indications that the initiation of the process takes place invariably in the less stable segments of a protein domain. We have studied the sequence and structural conservation of beta(2)-microglobulin that de...

journal_title：Journal of molecular biology

authors： Benyamini H,Gunasekaran K,Wolfson H,Nussinov R

更新日期：2003-06-27 00:00:00

abstract：:TWINKLE is the helicase at the mitochondrial DNA (mtDNA) replication fork in mammalian cells. Mutations in the PEO1 gene, which encodes TWINKLE, cause autosomal dominant progressive external ophthalmoplegia (AdPEO), a disorder associated with deletions in mtDNA. Here, we characterized seven different AdPEO-causing mut...

journal_title：Journal of molecular biology

authors： Korhonen JA,Pande V,Holmlund T,Farge G,Pham XH,Nilsson L,Falkenberg M

更新日期：2008-03-28 00:00:00

abstract：:The genes for alpha-fetoprotein and albumin arose by duplication of an ancestral gene that contained three genetic domains. These domains were generated by the triplication of a primordial genetic domain composed of five exons or subdomains. That the primordial domain itself arose by amplification of a simpler sequenc...

journal_title：Journal of molecular biology

authors： Alexander F,Young PR,Tilghman SM

更新日期：1984-02-25 00:00:00

abstract：:The NusA transcription elongation protein, which binds RNA, contains sequences corresponding to the S1 and KH classes of identified RNA binding domains. An essential function in E. coli, NusA is also one of the host factors required for action of the N transcription antitermination protein of lambda. Tandem KH domains...

journal_title：Journal of molecular biology

authors： Zhou Y,Mah TF,Greenblatt J,Friedman DI

更新日期：2002-05-17 00:00:00

abstract：:Crystals of an inhibitor of trypsin and tissue plasminogen activator from seeds of the legume Erythrina caffra have been obtained by vapour diffusion. The crystals belong to the hexagonal space group P6(1)22 (or its enantiomorph P6(5)22) with cell parameters 73.4 A and 143.0 A. There is one molecule in the asymmetric ...

journal_title：Journal of molecular biology

authors： Onesti S,Lloyd LF,Maeder D,Mistry A,Brick P

更新日期：1989-11-05 00:00:00

abstract：:Assembly of normally soluble proteins into ordered aggregates, known as amyloid fibrils, is a cause or associated symptom of numerous human disorders, including Alzheimer's and the prion diseases. Here, we test the ability of discontinuous molecular dynamics (DMD) simulations based on PRIME20, a new intermediate-resol...

journal_title：Journal of molecular biology

authors： Wagoner VA,Cheon M,Chang I,Hall CK

更新日期：2012-03-02 00:00:00

abstract：:As part of an overall effort to map the energetic landscape of the base excision repair pathway, we report the first thermodynamic characterization of repair enzyme binding to lesion-containing duplexes. Isothermal titration calorimetry (ITC) in conjunction with spectroscopic measurements and protease protection assay...

journal_title：Journal of molecular biology

authors： Minetti CA,Remeta DP,Zharkov DO,Plum GE,Johnson F,Grollman AP,Breslauer KJ

更新日期：2003-05-16 00:00:00

abstract：:The 37/ 67-kDa human laminin receptor (LamR) is a cell surface receptor for laminin, prion protein, and a variety of viruses. Because of its wide range of ligands, LamR plays a role in numerous pathologies. LamR overexpression correlates with a highly invasive cell phenotype and increased metastatic ability, mediated ...

journal_title：Journal of molecular biology

authors： Jamieson KV,Hubbard SR,Meruelo D

更新日期：2011-01-07 00:00:00

abstract：:During protein biosynthesis in bacteria, one of the earliest events that a nascent polypeptide chain goes through is the co-translational enzymatic processing. The event includes two enzymatic pathways: deformylation of the N-terminal methionine by the enzyme peptide deformylase (PDF), followed by methionine excision ...

journal_title：Journal of molecular biology

authors： Bhakta S,Akbar S,Sengupta J

更新日期：2019-03-29 00:00:00

abstract：:Expansion of the polyglutamine (polyQ) region in the protein ataxin-3 is associated with spinocerebellar ataxia type 3, an inherited neurodegenerative disorder that belongs to the family of polyQ diseases. Increasing evidence indicates that protein aggregation and fibre formation play an important role in these pathol...

journal_title：Journal of molecular biology

authors： Masino L,Nicastro G,Menon RP,Dal Piaz F,Calder L,Pastore A

更新日期：2004-12-03 00:00:00

abstract：:We have applied the method of simulated annealing to the refinement of the 3 A resolution crystal structure of the influenza virus hemagglutinin glycoprotein, using the program X-PLOR. Two different methods were introduced into X-PLOR to treat the non-crystallographic symmetry present in this and in other crystal stru...

journal_title：Journal of molecular biology

authors： Weis WI,Brünger AT,Skehel JJ,Wiley DC

更新日期：1990-04-20 00:00:00

abstract：:Transposable-element-mediated fusion of the conjugal plasmid pOX38::Tn9 with pBR322 results in the appearance of cointegrates composed of a single copy of each plasmid, and cointegrates which carry a single copy of pOX38 but multiple tandem copies of pBR322. These plasmids are separated by directly repeated copies of ...

journal_title：Journal of molecular biology

authors： Chandler M,Galas DJ

更新日期：1983-03-25 00:00:00

abstract：:By using a set of 476 ordered DNA clones (in lambda phage vector) that covers the entire chromosome of Escherichia coli K12, we have made an exhaustive survey of tRNA genes in the E. coli genome. Ultraviolet-irradiated bacteria were separately infected with each of the 476 clones and the RNA molecules produced upon in...

journal_title：Journal of molecular biology

authors： Komine Y,Adachi T,Inokuchi H,Ozeki H

更新日期：1990-04-20 00:00:00

abstract：:DNA replication fork arrest during the termination phase of chromosome replication in Bacillus subtilis is brought about by the replication terminator protein (RTP) bound to specific DNA terminator sequences (Ter sites) distributed throughout the terminus region. An attractive suggestion by others was that crucial to ...

journal_title：Journal of molecular biology

authors： Duggin IG,Andersen PA,Smith MT,Wilce JA,King GF,Wake RG

更新日期：1999-03-12 00:00:00

abstract：:The nucleotide sequence of the 3D (polymerase) gene of eight epidemiologically related isolates of foot-and-mouth disease virus of serotype C1 is reported. The genetic heterogeneity of 3D RNA is compared with that of the VP1-coding RNA of the same viruses. Regression lines of substitutions per nucleotide that distingu...

journal_title：Journal of molecular biology

authors： Villaverde A,Martínez-Salas E,Domingo E

更新日期：1988-12-05 00:00:00

abstract：:Meiotic clade AAA ATPases (ATPases associated with diverse cellular activities), which were initially grouped on the basis of phylogenetic classification of their AAA ATPase cassette, include four relatively well characterized family members, Vps4, spastin, katanin and fidgetin. These enzymes all function to disassemb...

journal_title：Journal of molecular biology

authors： Monroe N,Hill CP

更新日期：2016-05-08 00:00:00

abstract：:We report the X-ray structure analysis and refinement at 1.9 A resolution of calf gamma-II crystallin, a lens-specific protein. The sequence of Croft (1972) has been modified to give a polypeptide chain of 174 residues (cf. 165). The protein has a symmetrical, hierarchical structure of two globular domains each compri...

journal_title：Journal of molecular biology

authors： Wistow G,Turnell B,Summers L,Slingsby C,Moss D,Miller L,Lindley P,Blundell T

更新日期：1983-10-15 00:00:00