Abstract:
AIMS:To demonstrate expression of immunoglobulin light chain mRNA in diagnostic fine needle aspiration (FNA) cytology specimens using an in situ hybridisation (ISH) technique; and to evaluate ISH in a series of reactive lymphoid proliferations and malignant lymphomas. METHODS:Forty diagnostic FNA specimens showing a lymphoid cell population were examined for immunoglobulin light chain mRNA expression using ISH. Aspirates were obtained from lymph node (n = 34), salivary gland (n = 3), subcutaneous tissue, thyroid and breast (n = 1 each). The cases included 20 B cell lymphomas, five cases of Hodgkin's disease and 15 reactive lymphoid proliferations. Comparison with light chain immunoreactivity was made in 36 cases and histological correlation from biopsy material was available in 24. RESULTS:Immunoglobulin light chain restriction was demonstrated in 14 of 20 B cell lymphomas using ISH and in six of 17 B cell lymphomas using immunocytochemistry. A polytypic pattern of light chain expression was observed in four of five cases of Hodgkin's disease with both techniques, and in 12 of 15 and 11 of 14 reactive lymphoid proliferations using ISH and immunocytochemistry, respectively. CONCLUSIONS:The assessment of immunoglobulin light chain expression is a useful adjunct to morphology in the diagnosis of reactive and malignant lymphoid proliferations in FNA specimens. Light chain restriction can be shown using either immunocytochemistry or ISH, but the latter is more sensitive in the diagnosis of B cell lymphoma.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Stewart CJ,Farquharson MA,Kerr T,McCorriston Jdoi
10.1136/jcp.49.9.749subject
Has Abstractpub_date
1996-09-01 00:00:00pages
749-54issue
9eissn
0021-9746issn
1472-4146journal_volume
49pub_type
杂志文章abstract::An influenza virus vaccine containing the purified surface haemagglutinin and neuraminidase antigens of A/Victoria/75 and B/Hong Kong/73 viruses adsorbed to an aluminium hydroxide gel was assessed for reactogenicity and immunogenicity in children aged 4 to 11 years, since there is no influenza virus vaccine available ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.6.534
更新日期:1979-06-01 00:00:00
abstract:AIMS:The histopathological diagnosis of low-grade dysplasia (LGD) in Barrett's oesophagus (BO) is associated with poor interobserver agreement and guidelines dictate expert review. To facilitate nationwide expert review in the Netherlands, a web-based digital review panel has been set up, which currently consists of ei...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206511
更新日期:2021-01-01 00:00:00
abstract::Sera from 20 cases of toxoplasmic lymphadenopathy were examined by an enzyme linked immunosorbent assay toxoplasma IgG avidity (ELISA) at two laboratories. The results obtained were largely in agreement and showed that sera from patients with acute infection had low avidity IgG (30% or less), whereas sera from patient...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.12.1032
更新日期:1990-12-01 00:00:00
abstract:AIMS:To analyse the entity specific incidence and disease specific survival (DSS) of non-Hodgkin lymphomas (NHLs) in Tyrol/Austria, 1991-2000. METHODS:Data from 1307 NHLs (excluding primary cutaneous lymphomas and monoclonal gammopathies of undetermined significance) were obtained. Current status was available for all...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026815
更新日期:2006-01-01 00:00:00
abstract::The Scottish Meningococcus and Pneumococcus Reference Laboratory provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. The main tests used for the laboratory confirmation of meningococcal disease are culture, the polymerase chain reaction (PCR), antibody tes...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.7.556
更新日期:2001-07-01 00:00:00
abstract:AIMS:To determine whether myelodysplastic syndromes (MDS) are associated with sea blue histiocytosis in the bone marrow. METHODS:A retrospective review of bone marrow aspirates from 35 patients presenting consecutively with MDS and from 20 patients with each of the following: normal marrow appearance (routine staging ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.11.1030
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND:Friend leukaemia integration-1 (FLI-1) antibody is a useful marker for Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET) and vascular tumours. However, it is also expressed in subsets of lymphoblastic lymphoma, Merkel cell carcinoma (MCC) and desmoplastic small round cell tumour (DSRCT). AIM:To de...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.039230
更新日期:2007-06-01 00:00:00
abstract::Streptex was compared to routine laboratory identification methods available. The results from Streptex sometimes required several attempts before final identification could be achieved. In the main, group D streptococci other than Strep. faecalis failed to group with the Streptex antisera, and this method cannot ther...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.12.1234
更新日期:1979-12-01 00:00:00
abstract:AIMS/BACKGROUND:Telomerase is an enzyme that is expressed in most human neoplasms and is associated with tumour immortality. Determination of the point in neoplastic transformation at which telomerase is expressed may aid the understanding of tumour pathogenesis and progression. Despite numerous reports on telomerase, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.4.284
更新日期:1998-04-01 00:00:00
abstract::Escherichia coli O157:H7 was isolated from a fatal case of haemorrhagic colitis with haemolytic uraemic syndrome and neurological symptoms. This strain induced diarrhoea and neurological symptoms including incoordination, ataxia, and convulsions in piglets after oral inoculation. Similar neurological signs were seen i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.10.1099
更新日期:1988-10-01 00:00:00
abstract::Angiogenesis plays a crucial role in melanoma metastasis and progression. In recent years, numerous studies have investigated the prognostic and clinical significance of this phenomenon, and the development of molecular techniques has enabled us to achieve a better understanding of angiogenesis in melanoma. Herein, we...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2015-203482
更新日期:2016-06-01 00:00:00
abstract::One hundred and twenty-one rectal biopsies from 99 patients with carcinoma of the rectum or lower sigmoid colon were investigated using a high iron-diamine-Alcian blue technique for sulphated and non-sulphated acid mucins. It was found that in normal rectal mucosa sulphomucins are the main carbohydrate component of th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.2.123
更新日期:1972-02-01 00:00:00
abstract:AIMS:The generation and characterisation of a monoclonal antibody that specifically recognises the mdr-1 encoded protein, P-glycoprotein (P-170), on routinely processed formalin fixed, paraffin wax embedded tissue sections. METHODS:The monoclonal antibody, designated 6/1C, was produced following a combination of in vi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.6.465
更新日期:1997-06-01 00:00:00
abstract::A 65-year-old woman with a history of a left heminephrectomy for renal carcinoma developed hypercalcaemia 11 years after the operation. The same kidney was found to contain a recurrent renal carcinoma. After the radical nephrectomy of the left kidney, hypercalcaemia remitted but reappeared 11 months later. The right k...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.3.234
更新日期:1979-03-01 00:00:00
abstract:AIMS:Mitochondrial diseases form one of the largest groups of inborn errors of metabolism. The birth prevalence is approximately 1/5000 in well-studied populations, but little has been reported from Sub-Saharan Africa. The aim of this study was to describe the genetics underlying mitochondrial disease in South Africa. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-207026
更新日期:2020-10-28 00:00:00
abstract::Two cases of peritoneal granulomatous reactions to food starch are described. They followed bowel perforation and clinically mimicked tuberculous and glove-powder starch peritonitis. Their histological differences from corn-starch peritonitis warrant attention in the absence of previous documentation of starch as a co...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.4.435
更新日期:1983-04-01 00:00:00
abstract:AIMS:To study the pathogenicity and virulence characteristics of Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus sapro-phyticus. METHODS:BALB/c mice were challenged intraperitoneally with graded doses of three strains belonging to each species. LD50s were measured for each strain. Haemolysi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.8.743
更新日期:1994-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the protein expression of connexin 43 (Cx43) in primary urothelial bladder cancer and test its association with the histopathological characteristics and clinical outcome. METHODS:A tissue microarray containing 348 tissue samples from 174 patients with primary urothelial carcinomas of the bladde...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-202898
更新日期:2015-10-01 00:00:00
abstract::Experiments are described with glass fibre filter paper and complete glass filters as fitted to high pre-vacuum autoclaves to determine whether organisms can grow or penetrate through the filters. A filter consisting of four layers of glass fibre paper fitted inside a sterilizer chamber for a year and used for 96 ster...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.3.259
更新日期:1963-05-01 00:00:00
abstract::A case of idiopathic acquired sideroblastic anaemia transforming to acute myelofibrosis is reported. The appearance of atypical megakaryocytic proliferation in idiopathic acquired sideroblastic anaemia may presage the development of an acute myelofibrotic phase of this usually chronic disease. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.9.1032
更新日期:1984-09-01 00:00:00
abstract:AIMS:Sentinel lymph node (SLN) biopsy is the preferred surgical technique for staging the axilla in clinically node-negative breast cancer. Accurate intraoperative staging allows for the immediate performance of an axillary clearance in node-positive patients. We assessed the Metasin assay for the intraoperative analys...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203728
更新日期:2017-02-01 00:00:00
abstract:AIMS:To investigate staining patterns for mannan binding protein (MBP) by immunocytochemistry in liver biopsy specimens from patients with various hepatic disorders; to measure the serum MBP concentration in the patients at the time of biopsy; and to compare these to define further the role of MBP in disease. METHODS:...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.3.259
更新日期:1993-03-01 00:00:00
abstract:AIMS:To study the incidence, investigation, and management of severe hyponatraemia (serum sodium < 120 mmol/litre) over a period of six months in a district general hospital. METHODS:The laboratory computer was used to identify all inpatients who had a serum sodium concentration of less than 120 mmol/litre over a six ...
journal_title:Journal of clinical pathology
pub_type: 评论,杂志文章
doi:10.1136/jcp.55.12.893
更新日期:2002-12-01 00:00:00
abstract::During each of the four-year periods 1971-1975 and 1975-1979, the annual incidence of hepatitis B infection has been assessed in 56 patients with haemophilia A by measuring plasma HBsAg, anti-HBs and anti-HBc levels. Infection rates of 7% and 9.5% per annum respectively were observed for each four-year period despite ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.5.577
更新日期:1983-05-01 00:00:00
abstract::Experience gained in the last four years concerning the capabilities and limitations of an 8K Elliott 903 (18-bit word) computer with magnetic tape backing store in the routine operation of a clinical chemistry laboratory is described. Designed as a total system, routine operation has latterly had to be confined to da...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.7.480
更新日期:1973-07-01 00:00:00
abstract::Of 30 children with bleeding disorders exposed to an index tuberculosis case, six developed primary pulmonary tuberculosis and two others became positive skin reactors. There was a significant correlation between the amount of replacement treatment received and the development of evidence of tuberculous infection. Onl...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.10.1163
更新日期:1985-10-01 00:00:00
abstract::Urines from patients with symptoms of urinary tract infection yielded mixed growths of different colony types of Escherichia coli. The different colony types were found to be variants of single infecting strains caused by mutation or by phase action. It is suggested that care should be exercised in the interpretation ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.9.728
更新日期:1975-09-01 00:00:00
abstract::Cell surface proteolysis is an important mechanism for generating biologically active proteins that mediate a range of cellular functions and contribute to biological processes such as angiogenesis. Although most studies have focused on the plasminogen system and matrix metalloproteinases (MMPs), recently there has be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2003.014472
更新日期:2004-06-01 00:00:00
abstract::Pathological findings are described in four cases of a new aminoaciduria in which homocystine is excreted in the urine. All the patients were mentally retarded children. Three of them presented diagnostic features of Marfan's syndrome. Necropsy on one case and biopsy findings in the others are described. Fatty change ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.4.427
更新日期:1964-07-01 00:00:00
abstract::The results of a histological study of normal osteoid tissue in man, the monkey, the dog, and the rat, using thin microtome sections of plastic-embedded undecalcified bone, are described. Osteoid tissue covers the entire bone surface, except for areas of active resorption, although the thickness of the layer of osteoi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.3.229
更新日期:1972-03-01 00:00:00