Abstract:
:Airway epithelial cells cultured at the air-liquid interface possess highly differentiated functions and structures compared with the cells cultured under immersion. We examined the oxidative metabolism and glycolysis in cow tracheal epithelial cells on Days 3, 6, 10, and 13, cultured under three different conditions: (1) immersion culture on porous filters with apical and basolateral feeding (IM), (2) air-exposed culture on porous filters with basolateral feeding, i.e., air-liquid interface culture (AI), and (3) conventional immersion culture in plastic dishes with apical feeding (DI). Lactate production was less in AI than in IM and DI on Day 3 through Day 13, whereas cellular adenosine triphosphate content and basal O2 consumption were greater. Ouabain-sensitive and ouabain-insensitive O2 consumption, and the uncoupled O2 consumption were also greater in AI. Cytosolic lactate dehydrogenase activities on Day 10 were lower in AI, whereas alpha-ketoglutarate dehydrogenase activities were higher. The increased oxidative metabolism in AI was more pronounced at the late phase of culture (Days 10 and 13). In contrast, glycolysis remained elevated during the experiment in IM and DI. These data suggest that (I) AI begins to promote oxidative metabolism from growth phase by the provision of adequate oxygenation, and then further shifts to oxidative metabolism with differentiation; and (2) apical feeding may be responsible for the disturbance of the development of the oxidative metabolism.
journal_name
Am J Respir Cell Mol Biolauthors
Kondo M,Tamaoki J,Sakai A,Kameyama S,Kanoh S,Konno Kdoi
10.1165/ajrcmb.16.1.8998080subject
Has Abstractpub_date
1997-01-01 00:00:00pages
62-8issue
1eissn
1044-1549issn
1535-4989journal_volume
16pub_type
杂志文章abstract::We have developed a method to harvest and culture Clara cells isolated from guinea pig lungs. Their identity was confirmed by the presence of CC16kD protein specific for these cells; we studied their capacity to generate endothelin 1 (ET-1). Using monoclonal antibody and immunofluorescence techniques, ET-1 was localiz...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
abstract::Regulatory T cells (Tregs) play an essential role in maintaining the homeostatic balance of immune responses. Asthma is an inflammatory condition of the airways that is driven by dysregulated immune responses toward normally innocuous antigens. Individuals with asthma have fewer and less functional Tregs, which may le...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章,评审
doi:10.1165/rcmb.2009-0342TR
更新日期:2010-11-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2017-0308OC
更新日期:2018-09-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.19.5.3103
更新日期:1998-11-01 00:00:00
abstract::IL-27 is involved in inflammatory reactions. CXCL10 is an important chemokine contributing to airway inflammatory disease. In this study, we investigated whether IL-27 modulated the synthesis of CXCL10 in primary human lung fibroblasts (HLFs). HLFs were activated by IL-27 alone, or in combination with other cytokines....
journal_title:American journal of respiratory cell and molecular biology
pub_type: 临床试验,杂志文章
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2012-0090OC
更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1165/rcmb.2005-0378OC
更新日期:2006-05-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.20.5.3479
更新日期:1999-05-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.22.6.3927
更新日期:2000-06-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb/5.1.13
更新日期:1991-07-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.18.6.3070
更新日期:1998-06-01 00:00:00
abstract::Inhaled nitric oxide (NO) is an important new therapeutic agent used to treat pulmonary arterial hypertension in a variety of disease states. However, the effects of NO on cells in the lung are uncertain. Previously, we have shown that NO gas depresses neutrophil oxidative cell function and increases neutrophil cell d...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.18.3.2875
更新日期:1998-03-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.10.2.8110470
更新日期:1994-02-01 00:00:00
abstract::beta-glucans represent major structural components of fungal cell walls. We recently reported that Pneumocystis carinii beta-glucans stimulate alveolar macrophages to release proinflammatory cytokines. Macrophage activation by beta-glucan is augmented by serum, implying the presence of circulating factors that interac...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.25.2.4427
更新日期:2001-08-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2015-0047OC
更新日期:2016-05-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2012-0026OC
更新日期:2012-08-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2012-0039OC
更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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journal_title:American journal of respiratory cell and molecular biology
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更新日期:2015-10-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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更新日期:2014-03-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2003-0253OC
更新日期:2004-04-01 00:00:00
abstract::The objective of this study was to define phenotypic changes of antigen-presenting cells (APCs) and T cells in a murine model of antigen-induced airways inflammation that involves intraperitoneal sensitization with ovalbumin (OVA)/adjuvant followed by antigen aerosolization. We investigated the APC and T-cell compartm...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
abstract::The increase of airway smooth muscle (ASM) mass in asthma results from hypertrophic and hyperplastic stimuli, and leads to an increase in cellular contractile proteins. However, little evidence correlates the relative contributions of hypertrophic and hyperplastic muscle with functional effects on airway resistance. W...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2011-0293OC
更新日期:2012-04-01 00:00:00