Abstract:
:Recent advances in molecular biological techniques have increased our capability to distinguish the small differences in gene expression between subpopulations of cells found within specific tissues or tumor isolates. We use subtractive hybridization or subtractive cloning to generate information regarding genes that direct various aspects of mammalian embryonic development. The technique also is used to identify genes with specificity for particular tissues or cell types or those that regulate various processes in the cell. Also, subject to analysis is the aberrant expression of genes involved in tumorigenesis. Another use is analysis of subpopulations of cell types previously identified within individual solid tumors. We used subtractive cloning in the analysis of cell line subpopulations derived from the human pediatric tumor neuroblastoma. This has resulted in the identification of novel genes that may be useful in the study of this disease.
journal_name
Semin Pediatr Surgjournal_title
Seminars in pediatric surgeryauthors
Swendeman SL,La Quaglia MPsubject
Has Abstractpub_date
1996-08-01 00:00:00pages
149-54issue
3eissn
1055-8586issn
1532-9453journal_volume
5pub_type
杂志文章,评审abstract::Biliary atresia (BA) remains an enigmatic disease with a degree of etiologic heterogeneity. A number of variants can be defined clinically, and these include the syndromic group (typically BA splenic malformation), cystic BA, and cytomegalovirus (CMV) IgM +ve associated BA. The remainder, and still the largest group, ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2012.05.010
更新日期:2012-08-01 00:00:00
abstract::Children with anorectal malformations (ARMs) constitute a significant group within a pediatric surgery practice. In females, the most common ARM is an imperforate anus with a rectovestibular fistula, followed by an imperforate anus with a rectoperineal fistula and then cloacal anomalies. Some malformations, such as an...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章
doi:10.1053/j.sempedsurg.2009.11.019
更新日期:2010-05-01 00:00:00
abstract::Improvements in parenteral nutrition and supportive therapy have led to a growing population of patients who survive for prolonged periods with short bowel syndrome. Definitive treatment for these patients requires innovative therapy based on a sound knowledge of small intestinal physiology and adaptation. Current und...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:
更新日期:1995-02-01 00:00:00
abstract::The pathogenesis of necrotizing enterocolitis (NEC) is poorly understood, but appears to be multifactorial and highly associated with immaturity of the gastrointestinal tract, colonization of the intestinal microbiota, and immature innate immune system. The goal of this review is to provide an overview of some of thes...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2005.05.001
更新日期:2005-08-01 00:00:00
abstract::Congenital obstructive uropathy is a relatively common developmental malformation, and severely affected fetuses die soon after birth from oligohydramnios-induced pulmonary hypoplasia or renal failure. Prenatal ultrasonography can reliably diagnose the specific anatomic defect, and using fetal urine sampling we can de...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:
更新日期:1993-05-01 00:00:00
abstract::Survey methodologies enable researchers to obtain information not routinely captured in electronic medical records, including how delivery of surgical care is perceived through the lens of a child, a parent or caregiver, or a surgeon. Here we describe the approach to using surveys to aid pediatric surgery practice. Th...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2018.10.007
更新日期:2018-12-01 00:00:00
abstract::Hirschsprung disease affects many children every year around the world. Currently, there is an extensive menu of diagnostic methods, and surgical treatments. This situation compels the physicians to follow the rationale of these interventions. The comprehensive diagnosis and treatment of Hirschsprung disease need sing...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2019.04.013
更新日期:2019-06-01 00:00:00
abstract::Nutritional management of infants and children differs from that of adults because of the extra requirements for growth and the limitations of physiological immaturity. Although parenteral nutrition (PN) is an accepted practice and a potentially life-saving therapy for pediatric patients who cannot be fed through thei...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:
更新日期:1992-08-01 00:00:00
abstract::When a female is born and has only a single perineal orifice on the newborn clinical examination, a diagnosis of cloaca type of anorectal malformation is made. Along with associated malformations which may initiate the ordering of radiologic imaging, there are a finite number of radiologic tests that are performed to ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2015.11.007
更新日期:2016-04-01 00:00:00
abstract::Appendectomy has been the standard of care for appendicitis since the late 1800s, and remains one of the most common operations performed in children. The advent of data-driven medicine has led to questions about every aspect of the operation-whether appendectomy is even necessary, when it should be performed (timing)...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2016.05.003
更新日期:2016-08-01 00:00:00
abstract::Central venous catheters (CVC) are commonplace in the management of critically ill patients and serve a variety of purposes. Venous access is important for the administration of fluid and medications as well as blood procurement needed for lab analyses. However, not every critically ill patient requires a CVC. This ar...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2019.01.005
更新日期:2019-02-01 00:00:00
abstract::The care provided in the postanesthesia recovery unit has become much more sophisticated in recent years. We address what type of care should be provided and what equipment is required to provide such care in the present day pediatric postanesthesia care unit. In this regard a wide variety of potential postanesthetic ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:
更新日期:1992-02-01 00:00:00
abstract::Parasitic infestation is common in developing countries especially in Africa. Children are often more vulnerable to these infections. Many health problems result from these infestations, including malnutrition, iron-deficiency anemia, surgical morbidities, and even impaired cognitive function and educational achieveme...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2012.01.009
更新日期:2012-05-01 00:00:00
abstract::The incidence and prevalence of childhood-onset inflammatory bowel diseases (IBD), including subtypes Crohn's disease and ulcerative colitis, have risen dramatically in recent years, and have emerged globally as important pediatric chronic diseases. Therefore, health care providers are more frequently encountering ver...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2017.10.010
更新日期:2017-12-01 00:00:00
abstract::Most children with short bowel syndrome experience spontaneous small bowel adaptation over time. This allows the majority to be weaned from parenteral nutrition. There are, however, some children who cannot be weaned and are potential candidates for techniques to promote intestinal adaptation and intestinal lengthenin...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/spsu.2001.22855
更新日期:2001-05-01 00:00:00
abstract::After a short description of mediastinal anatomy and embryology, the authors illustrate the division of the mediastinum, based on criteria of either surgical anatomy or radiological anatomy. Lastly, they discuss the topographical distribution of the mediastinal masses in anterior, visceral, and posterior compartments ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章
doi:10.1016/s1055-8586(99)70018-1
更新日期:1999-05-01 00:00:00
abstract::The importance of having a reproducible scoring system to objectively assess the severity of fecal incontinence and its response to treatment is stressed, and a number of methods are described. The approach to conservative treatment and investigation is outlined. Recent advances in the surgical management of fecal inc...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/spsu.2002.31806
更新日期:2002-05-01 00:00:00
abstract::Prune belly is a lax, wrinkled abdominal wall and frequently is associated with other anomalies. The prune belly syndrome is a specific constellation of anomalies consisting of an abdominal wall deficient in muscular tissue, dilated urinary tract, and bilateral cryptorchidism. This group of anomalies is also called th...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/spsu.2000.7556
更新日期:2000-08-01 00:00:00
abstract::The treatment of children who have major abdominal injuries has changed significantly during the past 2 decades. Surgical restraint has been the theme, and increased awareness of anatomic patterns and physiological responses has prompted successful nonoperative care of many solid organ injuries in children. The contri...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:
更新日期:1995-05-01 00:00:00
abstract::Hirschsprung (HSCR) disease is a relatively common neonatal developmental disorder of the enteric nervous system and is characterized by the absence of ganglion cells in the myenteric and submucosal plexuses of the distal intestine. This results in absent peristalsis in the affected bowel, and the development of a fun...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章
doi:10.1053/j.sempedsurg.2004.10.027
更新日期:2005-02-01 00:00:00
abstract::An examination of and recommendations regarding the approval process for medical devices are presented. The typical pathways and hurdles laid out by the Federal Food and Drug Administration (FDA) are discussed, and options for marketing and use of medical devices are addressed. The first step in the regulatory process...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2006.07.009
更新日期:2006-11-01 00:00:00
abstract::Vascular liver tumors in the pediatric population can present a diagnostic dilemma. The most common hepatic vascular tumors are hepatic hemangiomas; however the differential diagnosis can also include other benign lesions and malignant masses. Management is unique to the type and nature of the specific lesion. Thus, c...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.sempedsurg.2020.150970
更新日期:2020-10-01 00:00:00
abstract::Hypertrophic pyloric stenosis (HPS) is a common condition affecting infants that presents with progressive projectile nonbilious vomiting. The pyloric portion of the stomach becomes abnormally thickened and manifests as gastric outlet obstruction. The cause is unknown. Pyloromyotomy remains the standard of treatment a...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2006.10.004
更新日期:2007-02-01 00:00:00
abstract::Congenital tracheobronchial stenosis is a rare disease characterized by complete tracheal rings that can affect variable lengths of the tracheobronchial tree. It causes high levels of morbidity and mortality both due to the stenosis itself and to the high incidence of other associated congenital malformations. Success...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2016.02.007
更新日期:2016-06-01 00:00:00
abstract::After a diagnosis of omphalocele during pregnancy, questions regarding long-term prognosis are of primary importance for parents. It is imperative that their questions are answered with substantiated data to promote confident decisions for their children. They frequently express concerns regarding long-term survival, ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2019.04.004
更新日期:2019-04-01 00:00:00
abstract::Conventional treatment of respiratory failure involves positive pressure ventilation with high concentrations of inspired oxygen. If adequate gas exchange still cannot be achieved extracorporeal membrane oxygenation (ECMO) may be an option. The general indication for ECMO for respiratory insufficiency is a reversible ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2007.10.006
更新日期:2008-02-01 00:00:00
abstract::Disorders of sexual development (DSDs) are relatively rare congenital conditions in which the development of the chromosomal, gonadal, or anatomic sex is atypical. Some conditions may not manifest until puberty or adulthood. The examination and workup of either an infant or an older patient with suspected DSD should b...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2015.01.009
更新日期:2015-04-01 00:00:00
abstract::Myelomeningocele (MMC) is a common birth defect that is associated with significant lifelong morbidity. Little progress has been made in the postnatal surgical management of the child with spina bifida. Postnatal surgery is aimed at covering the exposed spinal cord, preventing infection, and treating hydrocephalus wit...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1016/s1055-8586(03)00029-5
更新日期:2003-08-01 00:00:00
abstract::Although most patients with esophageal atresia (EA) and tracheo-esophageal fistula (TEF) may benefit from "standard" management, which is deferred emergency surgery, some may present unexpected elements that change this paradigm. Birth weight, associated anomalies, and long gap can influence the therapeutic schedule o...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章,评审
doi:10.1053/j.sempedsurg.2014.09.006
更新日期:2014-10-01 00:00:00
abstract::Colorectal disease profiles for children in low- and middle-income settings (LMIC) are characterized by late presentation, increased complications and limited follow-up in many cases. There is a high prevalence of infectious conditions causing secondary colorectal disease such as Mycobacterium Tuberculosis(TB), Human ...
journal_title:Seminars in pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.sempedsurg.2020.150989
更新日期:2020-12-01 00:00:00