Venous antithrombotic and anticoagulant activities of a fucoïdan fraction.

Abstract:

:Fucoïdans catalyse thrombin inhibition by antithrombin (AT) and heparin cofactor II (HCII); their affinity for each serpin varies according to the seaweed species from which they are extracted, as well as their chemical composition and molecular weight. We extracted a homogeneous fucoïdan fraction from Ascophyllum nodosum, a brown seaweed, and tested its anticoagulant and antithrombotic activities. At a fucoïdan concentration of 3.75 micrograms/ml, thrombin inhibition mediated by AT showed an apparent second-order rate constant (kapp) of 2 x 10(8) M-1 min-1, compared to 1.5 x 10(6) M-1 min-1 for the uncatalyzed reaction. The kapp value of thrombin inhibition via HCII was 1.17 x 10(9) M-1 min-1 at a fucoïdan concentration of 50 micrograms/ml, compared to 1.72 x 10(5) M-1 min-1 for the uncatalyzed reaction. In a Wessler model of venous thrombosis, the fucoïdan fraction, injected intravenously to rabbits 10 min before thrombosis induction, exhibited antithrombotic activity: 1.8 mg/kg was the dose which inhibited F Xa-induced thrombus formation by 80% (ED80), compared to a heparin ED80 of 0.1 mg/kg. At this ED80 the antithrombotic effect of the fucoïdan persisted longer than that of heparin (30 min versus 15 min). The thrombin clotting time (TCT) was significantly prolonged (73 s versus control 29 s, compared to 53 s with heparin) 10 min after a fucoïdan bolus infusion giving a plasma fucoïdan concentration of 14.6 +/- 2.7 micrograms/ml. The bleeding time was slightly increased after fucoïdan infusion at the ED80. Fucoïdan extracted from marine flora thus shows promise as an antithrombotic drug.

journal_name

Thromb Haemost

authors

Mauray S,Sternberg C,Theveniaux J,Millet J,Sinquin C,Tapon-Bretaudière J,Fischer AM

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

1280-5

issue

5

eissn

0340-6245

issn

2567-689X

journal_volume

74

pub_type

杂志文章
  • A comparative double-blind, randomised trial of a new second generation LMWH (bemiparin) and UFH in the prevention of post-operative venous thromboembolism. The Bemiparin Assessment group.

    abstract::A randomised, prospective, double-blind trial was performed, to compare the safety and efficacy of a new low-molecular-weight heparin (LMWH) Bemiparin and standard unfractionated heparin (UFH), for the prophylaxis of postoperative venous thromboembolism. 300 patients scheduled to undergo elective hip arthroplasty were...

    journal_title:Thrombosis and haemostasis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Kakkar VV,Howes J,Sharma V,Kadziola Z

    更新日期:2000-04-01 00:00:00

  • Expression of vascular endothelial growth factor in human monocyte/macrophages stimulated with lipopolysaccharide.

    abstract::Vascular endothelial growth factor (VEGF) is a mitogen for endothelial cells. We have studied the production of VEGF by human macrophages in response to lipopolysaccharide (LPS). Macrophages stimulated with LPS expressed VEGF mRNA and protein in concentration- and time-dependent manners. The LPS-induced expression of ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Itaya H,Imaizumi T,Yoshida H,Koyama M,Suzuki S,Satoh K

    更新日期:2001-01-01 00:00:00

  • Lysophosphatidic acid activates nuclear factor kappa B and induces proinflammatory gene expression in endothelial cells.

    abstract::The cellular phospholipid, lysophosphatidic acid (LPA), released by activated platelets and fibroblasts or, at high levels, from ovarian and cervical carcinomas is a powerful serum mitogen that may modulate several signaling pathways in endothelial cells (EC). Hence, LPA could function in a paracrine manner during EC-...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Palmetshofer A,Robson SC,Nehls V

    更新日期:1999-11-01 00:00:00

  • Pharmacokinetics and Pharmacodynamics of Emicizumab in Persons with Hemophilia A with Factor VIII Inhibitors: HAVEN 1 Study.

    abstract::Emicizumab, a bispecific monoclonal antibody, bridges activated factor IX (FIXa) and FX, replacing the function of missing FVIIIa to restore effective hemostasis in persons with hemophilia A (PwHA). Here we assess pharmacokinetic (PK) and pharmacodynamic (PD) biomarkers in PwHA with FVIII inhibitors in the Phase III H...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1055/s-0040-1717114

    authors: Schmitt C,Adamkewicz JI,Xu J,Petry C,Catalani O,Young G,Negrier C,Callaghan MU,Levy GG

    更新日期:2020-10-21 00:00:00

  • Improvement of fibrin clot structure after factor VIII injection in haemophilia A patients treated on demand.

    abstract::Patients with haemophilia A have seriously impaired thrombin generation due to an inherited deficiency of factor (F)VIII, making them form unstable fibrin clots that are unable to maintain haemostasis. Data on fibrin structure in haemophilia patients remain limited. Fibrin permeability, assessed by a flow measurement ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH13-06-0479

    authors: Antovic A,Mikovic D,Elezovic I,Zabczyk M,Hutenby K,Antovic JP

    更新日期:2014-04-01 00:00:00

  • Fibrin glue for wound repair: facts and fancy.

    abstract::In wound repair, fibrin has a multiplicity of activities, some of which are intrinsic to the protein itself and some attributable to other blood constituents associated with the fibrin clot. Fibrin sealants, which have been approved for hemostasis in the US and Europe, are occasionally used wounds to promote healing. ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:10.1160/TH03-08-0526

    authors: Clark RA

    更新日期:2003-12-01 00:00:00

  • The clearance of human fibrinogen fragments X and Y in mice: a process mediated by the fragment D receptor.

    abstract::The clearance of radiolabeled human fibrinogen fragments X and Y was studied in the mouse model. Fragment X cleared rapidly from the circulation with less than 10% of the ligand remaining in the circulation at 4 hr. The clearance of fragment Y was somewhat slower, but was identical to the rate of clearance of fragment...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Pasqua JJ,Pizzo SV

    更新日期:1983-04-28 00:00:00

  • European Concerted Action on Anticoagulation (ECAA): International Normalized Ratio variability of CoaguChek and TAS point-of-care testing whole blood prothrombin time monitors.

    abstract::The object was to assess the variability in displayed International Normalised Ratio (INR) between monitors of the same manufacture using whole blood samples from the same subjects. Two brands of monitor, CoaguChek Mini and the TAS PT-NC were tested. 14 instruments of each brand were tested on the same day at the same...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Poller L,Keown M,Chauhan N,Shiach C,van den Besselaar AM,Tripodi A,Jespersen J,European Concerted Action on Anticoagulation.

    更新日期:2002-12-01 00:00:00

  • Genistein alters coagulation gene expression in ovariectomised rats treated with phytoestrogens.

    abstract::Recent data has shown that hormone therapy (HT) increases the risk of cardiovascular and thromboembolic disease, particularly in users of oral HT. Phytoestrogens are popular alternatives to oestrogen therapy; however, their effects on cardiovascular risk are unknown. We investigated the effect of the phytoestrogen, ge...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH10-03-0201

    authors: Kelly LA,O'Leary JJ,Seidlova-Wuttke D,Wuttke W,Norris LA

    更新日期:2010-12-01 00:00:00

  • Identification of dense granule specific membrane proteins in bovine platelets that are absent in the Chediak-Higashi syndrome.

    abstract::Platelets from cattle with the Chediak-Higashi syndrome (CHS) have a platelet dense granule deficiency. One hypothesis for the platelet dense granule deficiency is that the granule is simply not formed in CHS megakaryocytes (MK). Alternative hypotheses include that the granule is assembled in CHS MK but a functional a...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Meyers K,Seachord C

    更新日期:1990-10-22 00:00:00

  • Thrombin production, inactivation and expression during open heart surgery measured by assays for activation fragments including a new ELISA for prothrombin fragment F1 + 2.

    abstract::Activation of coagulation was studied during the peri-operative period in patients undergoing cardiopulmonary bypass (CPB) surgery using activation markers which have recently become available: prothrombin fragment F1 + 2 (F1 + 2), which is a measure of total thrombin generation, and thrombin-antithrombin complex, whi...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Boisclair MD,Lane DA,Philippou H,Sheikh S,Hunt B

    更新日期:1993-08-02 00:00:00

  • Pregnancy outcome in patients exposed to direct oral anticoagulants - and the challenge of event reporting.

    abstract::Today, direct oral anticoagulants (DOAC) are widely used alternatives to Vitamin-K antagonists (VKA). Women of reproductive age may become pregnant during anticoagulation and, while VKA carry an embryotoxic potential, the risk of DOAC embryopathy is unknown. As a result, some patients elect to terminate pregnancy for ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH16-04-0305

    authors: Beyer-Westendorf J,Michalski F,Tittl L,Middeldorp S,Cohen H,Abdul Kadir R,Arachchillage DJ,Arya R,Ay C,Marten S

    更新日期:2016-09-27 00:00:00

  • The effect of fibrin clots and clot-bound thrombin on the development of platelet procoagulant activity.

    abstract::We tested different types of clot for their ability to provoke procoagulant activity in platelets: normal clots from platelet poor plasma (des AABB- or fibrin II clots), similar clots in which the adsorbed thrombin has been inhibited by hirudin, and clots obtained by the action of two snake venom enzymes that release ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Kumar R,Béguin S,Hemker HC

    更新日期:1995-09-01 00:00:00

  • High-Content Immunophenotyping and Hierarchical Clustering Reveal Sources of Heterogeneity and New Surface Markers of Human Blood Monocyte Subsets.

    abstract:OBJECTIVE: Blood monocyte subsets are emerging as biomarkers of cardiovascular inflammation. However, our understanding of human monocyte heterogeneity and their immunophenotypic features under healthy and inflammatory conditions is still evolving. RATIONALE: In this study, we sought to investigate the immunophenome o...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1055/s-0039-1700871

    authors: Hoffmann J,Fišer K,Liebetrau C,Staubach N,Kost D,Voss S,Heiden AZ,Dörr O,Lipps C,Nef HM,Möllmann H,Hamm CW,Keller T,Troidl C

    更新日期:2020-01-01 00:00:00

  • Induction of vasoactive substances differs in LPS-induced and TF-induced DIC models in rats.

    abstract::We have investigated the role of two vasoactive substances, nitric oxide (NO)and endothelin (ET), in the pathophysiology of disseminated intravascular coagulation (DIC), using two types of DIC models. Experimental DIC was induced by sustained infusion of 0.1, 1, 10, or 50 mg/kg lipopolysaccharide (LPS), or 3.75 U/kg t...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Asakura H,Okudaira M,Yoshida T,Ontachi Y,Yamazaki M,Morishita E,Miyamoto K,Nakao S

    更新日期:2002-10-01 00:00:00

  • The Urokinase Plasminogen Activator System in Human Cancers: An Overview of Its Prognostic and Predictive Role.

    abstract::Urokinase plasminogen activator (uPA) system regulates extracellular matrix remodelling by activating ubiquitous protease plasmin in many important physiological processes. The system components include uPA, plasminogen activator inhibitors (PAIs) and uPA receptor (uPAR). Besides its role in physiological processes, u...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:10.1055/s-0038-1675399

    authors: Madunić J

    更新日期:2018-12-01 00:00:00

  • Home-diagnosis of deep venous thrombosis with impedance plethysmography.

    abstract::In order to assess the value of I.P.G. for the diagnosis of D.V.T. in general practice, an I.P.G. was carried out by a skilled technician in 255 consecutive patients with suspected D.V.T. at home. Ascending venography was carried out in 185 of these patients. In addition, blood for assay of AT III, platelet count, fib...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Peters SH,Jonker JJ,de Boer AC,den Ottolander GJ

    更新日期:1982-12-27 00:00:00

  • Increase in plasma concentration of plasminogen activator inhibitor, fibrinogen, von Willebrand factor, factor VIII:C and in erythrocyte sedimentation rate with age.

    abstract::Elderly patients have previously been shown to have an increased plasma concentration of tissue plasminogen activator (t-PA) antigen (t-PA Ag). Since the concentration of t-PA Ag depends on both free t-PA and t-PA complexed with inhibitors, mainly plasminogen activator inhibitor (PA inhibitor), we have investigated th...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Aillaud MF,Pignol F,Alessi MC,Harle JR,Escande M,Mongin M,Juhan-Vague I

    更新日期:1986-06-30 00:00:00

  • The effects of therapy with oestriol succinate and ethinyl oestradiol on the haemostatic mechanism in post-menopausal women.

    abstract::The effects on the haemostatic mechanism of oestrogen therapy, given to prevent bone loss in post-menopausal women, have been investigated. Oestriol succinate was given orally to 10 women at a level of 2 mg/day for 1 month and for a further 3 months with incremental increase of 2 mg each month. 6 of the 10 women were ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Davies T,Fieldhouse G,McNicol GP

    更新日期:1976-04-30 00:00:00

  • Natural history of mesenteric venous thrombosis in patients treated with vitamin K antagonists: a multi-centre, retrospective cohort study.

    abstract::Knowledge on the natural history of mesenteric vein thrombosis (MVT) and of the efficacy and safety of long-term oral anticoagulant therapy (OAT) in this setting is based on small uncontrolled series of patients with a limited follow-up. It was the aim of the study to assess the natural history of MVT in a cohort of p...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究

    doi:10.1160/TH08-12-0842

    authors: Dentali F,Ageno W,Witt D,Malato A,Clark N,Garcia D,McCool K,Siragusa S,Dyke S,Crowther M,WARPED consortium.

    更新日期:2009-09-01 00:00:00

  • Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface.

    abstract::Tissue factor pathway inhibitor (TFPI) is one of the main regulators of the tissue factor (TF) pathway of coagulation. To tether human TFPI to the cell surface, full length or truncated TFPI lacking the third Kunitz domain were fused with domains three and four and the carboxy-terminal sequence of human CD4. Construct...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Riesbeck K,Dorling A,Kemball-Cook G,McVey JH,Jones M,Tuddenham EG,Lechler RI

    更新日期:1997-12-01 00:00:00

  • The sensitivity and specificity of commercial reagents for the detection of lupus anticoagulant show marked differences in performance between photo-optical and mechanical coagulometers.

    abstract::This study was undertaken to appraise the application of those reagents most widely used in the UK for the detection and confirmation of lupus anticoagulant (LA) on an Amelung KC4A and a Sysmex CA-6000 coagulometer. Five sets of dilute Russell's viper venom time (DRVVT) reagents were assessed as well as the Textarin-P...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Lawrie AS,Mackie IJ,Purdy G,Machin SJ

    更新日期:1999-05-01 00:00:00

  • Extended anticoagulation for prevention of recurrent venous thromboembolism in carriers of factor V Leiden--cost-effectiveness analysis.

    abstract::There is much debate over the appropriateness of prescribing prolonged anticoagulation to heterozygous carriers of factor V Leiden suffering a first episode of deep vein thrombosis (DVT). We, thus used meta-analysis to estimate from six eligible studies the summary odds-ratio of recurrent DVT in carriers of factor V L...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Marchetti M,Pistorio A,Barosi G

    更新日期:2000-11-01 00:00:00

  • Effect of thrombin on phosphorylation of platelet membrane proteins.

    abstract::The effect of thrombin on the phosphorylating activity of platelet membranes was compared to that of trypsin. Preincubation of non-32P phosphorylated platelet membranes with or without either of these two enzymes resulted in a considerable loss of membrane protein kinase activity which was most severe when trypsin was...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Steiner M

    更新日期:1976-06-30 00:00:00

  • Binding of fibrinogen to cultured bovine endothelial cells.

    abstract::The binding properties of purified bovine fibrinogen to bovine aortic endothelial cells have been examined in a tissue culture system. Endothelial cells bound 125I-fibrinogen in a calcium dependent fashion. Removal of calcium by EDTA instantaneously detached most of the cell-associated fibrinogen. Binding of fibrinoge...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Delvos U,Preissner KT,Müller-Berghaus G

    更新日期:1985-02-18 00:00:00

  • Gelatinase B (MMP-9) deficiency does not affect murine adipose tissue development.

    abstract::This study was performed to follow up on the observation that gelatinase A (MMP-2) deficiency impairs adipose tissue development in mice. The aim was to evaluate the role of its functional homologue gelatinase B (MMP-9) in adipose tissue growth. MMP-9 antigen levels were determined in lean and in obese women before an...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH09-10-0739

    authors: Van Hul M,Piccard H,Lijnen HR

    更新日期:2010-07-01 00:00:00

  • Characterisation of epitopes on human tissue plasminogen activator recognised by a group of monoclonal antibodies.

    abstract::Seven mouse monoclonal antibodies have been produced against human melanoma tissue plasminogen activator (t-PA). They were specifically bound to 125I t-PA but not 125I urokinase (u-PA) and inhibited t-PA, but not u-PA, activity in plasminogen-rich 125I fibrin wells. Three of the antibodies directly inhibited the amido...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: MacGregor IR,Micklem LR,James K,Pepper DS

    更新日期:1985-02-18 00:00:00

  • A critical investigation into the existence of circulating platelet aggregates.

    abstract::By a method of counting single platelets in diluted whole blood, platelet aggregates were quantified ex-vivo. Four groups: 20 thrombotic patients, 10 non-thrombotic patients, 10 healthy old controls and 10 healthy young controls were included in the study. Using a 19 gauge needle, with and without tubing, venous blood...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Saniabadi AR,Lowe GD,Madhok R,Spowart K,Shaw B,Barbenel JC,Forbes CD

    更新日期:1986-08-20 00:00:00

  • Fibrillar cellular fibronectin supports efficient platelet aggregation and procoagulant activity.

    abstract::The ability of cellular fibronectin, found in the vessel wall in a fibrillar conformation, to regulate platelet functions and trigger thrombus formation remains largely unknown. In this study, we evaluated how parietal cellular fibronectin can modulate platelet responses under flow conditions. A fibrillar network was ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH14-11-0958

    authors: Maurer E,Schaff M,Receveur N,Bourdon C,Mercier L,Nieswandt B,Dubois C,Jandrot-Perrus M,Goetz JG,Lanza F,Gachet C,Mangin PH

    更新日期:2015-11-25 00:00:00

  • Atherosclerosis at arterial bifurcations: evidence for the role of haemodynamics and geometry.

    abstract::Atherosclerotic plaques are found at distinct locations in the arterial system, despite the exposure to systemic risk factors of the entire vascular tree. From the study of arterial bifurcation regions, emerges ample evidence that haemodynamics are involved in the local onset and progression of the atherosclerotic dis...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:10.1160/TH15-07-0597

    authors: Morbiducci U,Kok AM,Kwak BR,Stone PH,Steinman DA,Wentzel JJ

    更新日期:2016-03-01 00:00:00