Molecular mapping of the heparin-binding exosite of thrombin.

abstract：:Macrophage responses to Francisella infection have been characterized previously by subdued proinflammatory responses; however, these studies have generally focused on macrophage cell lines or monocyte-derived macrophages. Therefore, we studied the ability of fresh human blood monocytes to engulf and respond to Franci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gavrilin MA,Bouakl IJ,Knatz NL,Duncan MD,Hall MW,Gunn JS,Wewers MD

更新日期：2006-01-03 00:00:00

abstract：:Size-selective mortality inevitably leads to a decrease in population density and exerts a direct negative effect on targeted size classes. But density and population size structure are also shaped by food-dependent processes, such as individual growth, maturation, and reproduction. Mortality relaxes competition and t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schröder A,Persson L,de Roos AM

更新日期：2009-02-24 00:00:00

abstract：:Because RNAs fold as they are being synthesized, their transcription rate can affect their folding. Here, we report the results of single-molecule fluorescence studies that characterize the ligand-dependent cotranscriptional folding of the Escherichia coli thiM riboswitch that regulates translation. We found that the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uhm H,Kang W,Ha KS,Kang C,Hohng S

更新日期：2018-01-09 00:00:00

abstract：:Trial electron density functions have some conceptual and computational advantages over wave functions. The properties of some simple density functions for H(+) (2) and H(2) are examined. It appears that for a diatomic molecule a good density function would be given by rho = N(A(2) + B(2)), in which A and B are short ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearson RG,Palke WE

更新日期：1980-04-01 00:00:00

abstract：:A change-point detection is proposed by using a Bayesian-type statistic based on the shortest Hamiltonian path, and the change-point is estimated by using ratio cut. A permutation procedure is applied to approximate the significance of Bayesian-type statistics. The change-point test is proven to be consistent, and an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi X,Wu Y,Rao CR

更新日期：2017-04-11 00:00:00

abstract：:The capacity of Shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. Shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as interc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bernardini ML,Mounier J,d'Hauteville H,Coquis-Rondon M,Sansonetti PJ

更新日期：1989-05-01 00:00:00

abstract：:Regulation of irreversible cell lineage commitment depends on a delicate balance between positive and negative regulators, which comprise a sophisticated network of transcription factors. Receptor activator of NF-kappaB ligand (RANKL) stimulates the differentiation of bone-resorbing osteoclasts through the induction o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishikawa K,Nakashima T,Hayashi M,Fukunaga T,Kato S,Kodama T,Takahashi S,Calame K,Takayanagi H

更新日期：2010-02-16 00:00:00

abstract：:A mammalian A-type cyclin, cyclin A1, is highly expressed in testes of both human and mouse and targeted mutagenesis in the mouse has revealed the unique requirement for cyclin A1 in the progression of male germ cells through the meiotic cell cycle. While very low levels of cyclin A1 have been reported in the human he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao C,Wang XY,Wei HQ,Li SQ,Merghoub T,Pandolfi PP,Wolgemuth DJ

更新日期：2001-06-05 00:00:00

abstract：:Women outnumber men in undergraduate enrollments, but they are much less likely than men to major in mathematics or science or to choose a profession in these fields. This outcome often is attributed to the effects of negative sex-based stereotypes. We studied the effect of such stereotypes in an experimental market, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reuben E,Sapienza P,Zingales L

更新日期：2014-03-25 00:00:00

abstract：:Fetal cells, potentially usable for prenatal diagnosis, were sorted from maternal blood samples taken as early as 15 weeks of gestation. Immunogenetic and cytogenic criteria established the fetal origin of the observed cells: Y-chromatin-containing (male) cells were detected in the sorted sample if and only if the new...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herzenberg LA,Bianchi DW,Schröder J,Cann HM,Iverson GM

更新日期：1979-03-01 00:00:00

abstract：:The functional groups found among the RNA bases and in the phosphoribose backbone represent a limited repertoire from which to construct a ribozyme active site. This work investigates the possibility that simple RNA phosphodiester and hydroxyl functional groups could catalyze amide bond synthesis. Reaction of amine gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chamberlin SI,Merino EJ,Weeks KM

更新日期：2002-11-12 00:00:00

abstract：:Neurogenesis has recently been observed in the adult human brain, suggesting the possibility of endogenous neural repair. However, the augmentation of neurogenesis in the adult human brain in response to neuronal cell loss has not been demonstrated. This study was undertaken to investigate whether neurogenesis occurs ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curtis MA,Penney EB,Pearson AG,van Roon-Mom WM,Butterworth NJ,Dragunow M,Connor B,Faull RL

更新日期：2003-07-22 00:00:00

abstract：:We report here the reconstitution of a pathway that leads to the apoptotic changes in nuclei by using recombinant DNA fragmentation factor (DFF), a heterodimeric protein of 40 and 45 kDa. Coexpression of DFF40 and DFF45 is required to generate recombinant DFF, which becomes activated when DFF45 is cleaved by caspase-3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu X,Li P,Widlak P,Zou H,Luo X,Garrard WT,Wang X

更新日期：1998-07-21 00:00:00

abstract：:To investigate the chemical relationships between rat liver lysosomal and microsomal beta-D-glucuronidases (EC 3.2.1.31), which are essentially identical catalytically and in reactivity with antibody and similar in molecular weight, the two enzymes were isolated by procedures in which modifications of the proteins wer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tulsiani DR,Six H,Touster O

更新日期：1978-07-01 00:00:00

abstract：:To reduce greenhouse gas emissions from deforestation, Indonesia instituted a nationwide moratorium on new license areas ("concessions") for oil palm plantations, timber plantations, and logging activity on primary forests and peat lands after May 2011. Here we indirectly evaluate the effectiveness of this policy usin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busch J,Ferretti-Gallon K,Engelmann J,Wright M,Austin KG,Stolle F,Turubanova S,Potapov PV,Margono B,Hansen MC,Baccini A

更新日期：2015-02-03 00:00:00

abstract：:The mechanism of the proline-catalyzed aldol reaction has stimulated considerable debate, and despite limited experimental data, at least five different mechanisms have been proposed. Complementary to recent theoretical studies we have initiated an experimental program with the goal of clarifying some of the basic mec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： List B,Hoang L,Martin HJ

更新日期：2004-04-20 00:00:00

abstract：:Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the green alga Chlamydomonas reinhardtii revealed functions for specific xanthophylls in the nonradiative dissipation of exce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niyogi KK,Björkman O,Grossman AR

更新日期：1997-12-09 00:00:00

abstract：:The biosynthesis of fibronectin during the in vivo development of matrix-induced endochondral bone was investigated by using [35S]methionine in rats. The dmineralized bone matrix that was implanted subcutaneously to induce local bone formation bound circulating fibronectin. This may be an important initial requirement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss RE,Reddi AH

更新日期：1980-04-01 00:00:00

abstract：:The frequency of recombination between the G gamma-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing greater than 700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allele-specific oligon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cui XF,Li HH,Goradia TM,Lange K,Kazazian HH Jr,Galas D,Arnheim N

更新日期：1989-12-01 00:00:00

abstract：:The generation of high levels of new catalytic activities on natural and artificial protein scaffolds is a major goal of enzyme engineering. Here, we used random mutagenesis and selection in vivo to establish a sugar isomerisation reaction on both a natural (beta alpha)(8)-barrel enzyme and a catalytically inert chime...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Claren J,Malisi C,Höcker B,Sterner R

更新日期：2009-03-10 00:00:00

abstract：:Extracellular cAMP plays a crucial role in regulating the developmental program of Dictyostelium discoideum, functioning as a chemotactic agent, as well as a signal that regulates expression of developmentally expressed genes. These activities appear to be mediated by a cell-surface receptor for cAMP. We have studied ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chisholm RL,Hopkinson S,Lodish HF

更新日期：1987-02-01 00:00:00

abstract：:Calcium oscillations suppress mitochondrial movements along the microtubules to support on-demand distribution of mitochondria. To activate this mechanism, Ca(2+) targets a yet unidentified cytoplasmic factor that does not seem to be a microtubular motor or a kinase/phosphatase. Here, we have studied the dependence of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saotome M,Safiulina D,Szabadkai G,Das S,Fransson A,Aspenstrom P,Rizzuto R,Hajnóczky G

更新日期：2008-12-30 00:00:00

abstract：:Arginine methylation has emerged as a widespread and reversible protein modification with the potential to regulate a multitude of cellular processes, but its function is poorly understood. Endolysosomes play an important role in Wnt signaling, in which glycogen synthase kinase 3 (GSK3) becomes sequestered inside mult...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albrecht LV,Ploper D,Tejeda-Muñoz N,De Robertis EM

更新日期：2018-06-05 00:00:00

abstract：:Up-regulation of the folding machinery of the heat-shock protein 90 (Hsp90) chaperone protein is crucial for cancer progression. The two Hsp90 isoforms (α and β) play different roles in response to chemotherapy. To identify isoform-selective inhibitors of Hsp90(α/β)/cochaperone p23 interactions, we developed a dual-lu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan CT,Reeves RE,Geller R,Yaghoubi SS,Hoehne A,Solow-Cordero DE,Chiosis G,Massoud TF,Paulmurugan R,Gambhir SS

更新日期：2012-09-11 00:00:00

abstract：:Spreading depression (SD), a slow diffusion-mediated self-sustained wave of depolarization that severely disrupts neuronal function, has been implicated as a cause of cellular injury in a number of central nervous system pathologies, including blind spots in the retina. Here we show that in the hypoglycemic chicken re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Y,Santos LM,Mattiace LA,Costa ML,Ferreira LC,Benabou K,Kim AH,Abrahams J,Bennett MV,Rozental R

更新日期：2012-02-14 00:00:00

abstract：:Voltage-dependent potassium channels (Kv) are homotetramers composed of four voltage sensors and one pore domain. Because of high-level structural flexibility, the first mammalian Kv structure, Kv1.2 at 2.9 A, has about 37% molecular mass of the transmembrane portion not resolved. In this study, by applying a novel no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Wang Q,Ni F,Ma J

更新日期：2010-06-22 00:00:00

abstract：:Overexpression of human cyclooxygenase 2 (COX-2) in the mammary glands of transgenic mice induces tissue-specific tumorigenic transformation. However, the molecular mechanisms involved are not yet defined. Here we show that COX-2 expressed in the epithelial cell compartment regulates angiogenesis in the stromal tissue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang SH,Liu CH,Conway R,Han DK,Nithipatikom K,Trifan OC,Lane TF,Hla T

更新日期：2004-01-13 00:00:00

abstract：:Maintenance of genome stability is carried out by a suite of DNA repair pathways that ensure the repair of damaged DNA and faithful replication of the genome. Of particular importance are the repair pathways, which respond to DNA double-strand breaks (DSBs), and how the efficiency of repair is influenced by sequence h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer D,Fu BX,Heyer WD

更新日期：2015-12-15 00:00:00

abstract：:Time courses of 13C labeling from alanine and ethanol in perfused mouse livers have been followed by NMR. The enrichment at specific carbons of glucose, glutamate, glutamine, aspartate, acetate, acetoacetate, beta-hydroxybutyrate, and lactate has been measured. The specific labeling of glutamate in the presence of lab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen SM,Shulman RG,McLaughlin AC

更新日期：1979-10-01 00:00:00

abstract：:The use of fluorescent probes to give continuous readouts of the structural states of mitochondrial membranes during energy coupling seems a logical extension of their use in the study of protein structural changes. A clear correlation of the probes' fluorescence characteristics with the acquisition of energy coupling...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chance B

更新日期：1970-10-01 00:00:00