Abstract：
PURPOSE:This prospective study compared the effects of four sperm preparation methods on semen samples with normal sperm parameters. The samples were obtained from husbands of infertile couples being evaluated at a fertility center in New York City. Twenty-nine of 51 men in the study (56.9%) had fathered a child within the past 2-5 years. Specifically, the study compared the effects of simple wash (unselective) and three selective methods--conventional swim-up, three-gradient Percoll separation, and swim-up from the Percoll-separated fraction--on the concentration, percentage motile, percentage normal (oval) morphology, percentage intact acrosomes, percentage motile sperm recovery, and motile sperm concentration in the processed samples. The same parameters were measured in the untreated ejaculate, resulting in five data sets. These data were analyzed by both comparison across the five sets and pairwise multiple comparisons between sets. RESULTS:The study showed that of the selective methods, conventional swim-up and Percoll separation yielded comparably optimal percentages motile sperm recovery and motile sperm concentrations. CONCLUSION:However, swim-up yielded a significantly higher percentage oval and percentage motile sperm than Percoll, while Percoll had a significantly higher percentage acrosome-intact. Swim-up after Percoll separation yielded the highest percentage motility and percentage oval but had the lowest motile sperm concentration with the lowest percentage acrosome-intact.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Brandeis VT,Manuel MTdoi
10.1007/BF01228091subject
Has Abstractpub_date
1993-08-01 00:00:00pages
409-16issue
6eissn
1058-0468issn
1573-7330journal_volume
10pub_type
杂志文章abstract：BACKGROUND:In previous studies, higher progesterone secretion was observed in mature versus immature cumulusoocyte complexes. In mature cumulus mass that become homogeneously spread in culture (type C/D) progesterone secretion was higher than in partially (type B) or totally (type A) aggregated morphology. In sharp con...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02765828
更新日期：1997-05-01 00:00:00
abstract：:With the increased rate of stable remission after gonadotoxic cancer treatment, new methods of fertility preservation are required in order to provide the best possible care for oncological patients. Here, we report an original case of euploid blastocyst cryopreservation after in vitro maturation of ovarian tissue ooc...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01729-2
更新日期：2020-04-01 00:00:00
abstract：PURPOSE:The male factor is nowadays one of the major problems in the treatment of infertility. New methods of assisted fertilization such as the intracytoplasmic sperm injection (ICSI) show better fertilization and pregnancy rates than classical IVF. METHOD:In this study, we present a new technique of sperm preparatio...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02211143
更新日期：1995-08-01 00:00:00
abstract：:The study aims to discuss the effects of aging on the male reproductive system. A systematic review was performed using PubMed from 1980 to 2014. Aging is a natural process comprising of irreversible changes due to a myriad of endogenous and environmental factors at the level of all organs and systems. In modern life,...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
doi：10.1007/s10815-016-0663-y
更新日期：2016-04-01 00:00:00
abstract：PURPOSES:To investigate the frequencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. Moreover, to compare the prevalence of these abnormalities with other countries and regions in the world. METHODS:305 infertile men were enrolled. A complete semen analysis and reproduc...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-010-9420-9
更新日期：2010-07-01 00:00:00
abstract：:Gender selection by PGD is an appropriate use of medical resources. Children borne through PGD for gender determination would be welcome and would come into a couple's life at a planned, opportune time. If the practice were made more available through insurance coverage, the size and makeup of families could become a ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1016871925450
更新日期：2002-09-01 00:00:00
abstract：PURPOSE:Assessment of sperm morphology has been reconsidered since 2001 with the development of motile sperm organelle morphology examination (MSOME). This observation technique that combines high magnification microscopy and the Nomarski interference contrast makes it possible to select spermatozoa with as few vacuole...
journal_title：Journal of assisted reproduction and genetics
pub_type： 临床试验,杂志文章
doi：10.1007/s10815-015-0645-5
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:An association between the INS VNTR polymorphisms and polycystic ovary syndrome (PCOS) susceptibility has been reported in previous studies, but the results were inconsistent. This study was conducted to explore this association using meta-analysis. METHODS:PubMed, Embase, and China National Knowledge Infrastr...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,meta分析
doi：10.1007/s10815-014-0211-6
更新日期：2014-06-01 00:00:00
abstract：PURPOSE:Previous studies reported that patients with endometriosis had excess nitric oxide (NO) in the reproductive tract and poor embryo development in IVF cycles. This study aims to elucidate the effects of NO on early embryo development. METHODS:Zygotes from superovulated B6CBF1 mice were cultured to blastocysts in...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0045-7
更新日期：2013-08-01 00:00:00
abstract：PURPOSE:Artificial oocyte activation using calcium ionophores and enhancement of embryonic developmental potential by the granulocyte-macrophage colony-stimulating factor (GM-CSF) have already been reported. In this study, we evaluated the synergistic effect of these two methods on aged human unfertilized oocytes after...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-016-0823-0
更新日期：2017-01-01 00:00:00
abstract：PURPOSE:To evaluate the association of objectively measured physical activity (PA) and sedentary behaviour before and during in vitro fertilization (IVF) with controlled ovarian stimulation (COS) and pregnancy outcomes. METHODS:This longitudinal study involved 107 infertile women undergoing IVF treatment. PA and seden...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01864-w
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. METHODS:A total of 103 subjects visiting the infe...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0282-4
更新日期：2014-09-01 00:00:00
abstract：PURPOSE:The present study examined the effect of aging on female reproductive potential. METHODS:Six-week-old and 9-month-old CD1 mice were referred to as the 'young' and 'aged' groups, respectively. Oocytes were collected after superovulation, and their viability were compared using parthenogenetic activation. The an...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0160-5
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:To assess longitudinal trends in in vitro fertilization (IVF) patients' choices for disposing of cryopreserved embryos. METHODS:This is a retrospective cohort study of embryo disposition forms submitted between January 2000 and February 2020 at a university-based fertility clinic. Primary outcome was dispositi...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01927-y
更新日期：2020-11-01 00:00:00
abstract：PURPOSE:To perform complex preimplantation genetic tests (PGT) for aneuploidy screening, Robertsonian translocation, HLA-matching, and X-linked hyper IgM syndrome (XHIGM) caused by a novel mutation c.156 G>T of CD40LG gene. METHODS:Reverse transcription PCR (RT-PCR) and Sanger sequencing were carried out to confirm th...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01846-y
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:To investigate interactions on gestational age among two environmental risk factors and four maternal genetic polymorphisms: organic solvents, passive smoking, CYP1A1 rs4646903 (MspI), EPHX1 rs2234922 (His139Arg), GSTT1 and PON2 rs12026 (Ala148Gly). METHODS:A pregnant women cohort was conducted at Beijing Yans...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0256-6
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:A growing body of evidence suggests that microRNAs play fundamental regulatory roles in embryo implantation and maintenance of pregnancy. The aim of this study was to investigate the possible association between miR-146a C > G, miR-149 T > C, miR-196a2 T > C, and miR-499 A > G polymorphisms and genetic susce...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01573-z
更新日期：2019-11-01 00:00:00
abstract：PURPOSE:To compare the results of intracytoplasmic morphologically selected sperm injection (IMSI) between cycles in which the swim-up (SUP) or the density gradient centrifugation (DGC) techniques were used for sperm preparation. METHODS:We evaluated 70 IMSI cycles performed in women with age ≤ 37 years, undergoing IM...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-9989-x
更新日期：2013-06-01 00:00:00
abstract：PURPOSE:To elicit patient preferences for social media utilization and content in the infertility clinic. METHODS:This was a cross-sectional survey study conducted in three US fertility practices. Women presenting to the infertility clinic for an initial or return visit were offered an anonymous voluntary social media...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-018-1189-2
更新日期：2018-07-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate the best protocol to prepare endometrium for frozen embryo replacement (FER) cycles. METHODS:This study is a systematic review and meta-analysis. Following PubMed and OvidSP search, a total of 1166 studies published after 1990 were identified following removal of dupli...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s10815-016-0787-0
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:To investigate whether the success rate of ICSI is (1) related to the etiology of infertility or (2) adversely affected by a family history of potential genetic disorders. METHODS:All men with an ICSI indication in our hospital between 1994 and 2005 were included in our cohort study. Data on the ICSI process, ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-009-9315-9
更新日期：2009-06-01 00:00:00
abstract：PURPOSE:We previously reported the gene expression of epidermal growth factor (EGF) in the process of decidualization in the human endometrium. This study was undertaken to investigate the biological significance of transforming growth factor-alpha (TGF-alpha), which shares a significant sequence homology with EGF, in ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02766113
更新日期：1997-04-01 00:00:00
abstract：PURPOSE:The length of GT-repeats polymorphic region in the promoter of human Heme oxygenase-1 gene (HO-1) alters the level of its transcriptional activity in response to oxidative stresses. Decreased level of HO-1 protein in the seminal plasma has been reported to be associated with oligospermia and azoospermia in male...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-011-9574-0
更新日期：2011-08-01 00:00:00
abstract：PURPOSE:This prospective study was designed to investigate whether anti-Müllerian hormone (AMH) levels at basal and ovulation triggering day are associated with ovarian response and pregnancy outcome for in vitro fertilization (IVF). METHOD:60 infertility women undergoing IVF were prospectively studied. On day 3 of th...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-009-9332-8
更新日期：2009-07-01 00:00:00
abstract：PURPOSE:To evaluate the impact of subtle progesterone (P4) rise on the day of HCG on pregnancy outcome in ICSI patients stimulated with long agonist protocol. METHODS:One hundred forty-nine consecutive controlled ovarian hyperstimulation cycles for ICSI using long luteal agonist protocol. RESULTS:Mean serum progester...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-009-9309-7
更新日期：2009-05-01 00:00:00
abstract：PURPOSE:Few published articles have compared initial hCG values across all different types of ART cycles, including cycles with fresh or frozen embryo transfer. No articles have compared initial hCG values in cycles utilizing preimplantation genetic screening (PGS). The purpose of this study is to compare initial hCG v...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-017-0987-2
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:The aim was to assess the fertilizing capacity of spermatozoa cool-preserved in electrolyte-free (EF) solution. METHODS:Mouse spermatozoa were cool-preserved in EF solution and the acrosomal status of the spermatozoa was compared before and after preservation using chlortetracycline stain. Mouse oocytes were i...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1009449926015
更新日期：2000-08-01 00:00:00
abstract：PURPOSE:To investigate the influence of sperm DNA integrity on the zona binding ability of mouse spermatozoa in relation to their sex chromosomal constitution. METHOD(S):In this prospective experimental study, the sperm DNA fragmentation was induced by exposing testicular area of Swiss Albino mice (Mus musculus) to di...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0123-x
更新日期：2013-12-01 00:00:00
abstract：PURPOSE:To demonstrate that a euploid embryo derived from an oocyte with reciprocal aneuploid polar bodies is capable of producing a chromosomally normal child. METHODS:A case report of maternal MI error compensation where single nucleotide polymorphism (SNP) microarray based comprehensive chromosome screening (CCS) w...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-012-9746-6
更新日期：2012-06-01 00:00:00
abstract：PURPOSE:The aim of the present study was to improve the in vitro maturation (IVM) procedure using oocytes from surplus ovarian tissue after fertility preservation. METHODS:Twenty-five patients aged 17-37 years were included in the study. Maturation was compared between oocytes collected in HEPES-buffered medium or sal...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01724-7
更新日期：2020-04-01 00:00:00
