Abstract：
PURPOSE:The present study examined the effect of aging on female reproductive potential. METHODS:Six-week-old and 9-month-old CD1 mice were referred to as the 'young' and 'aged' groups, respectively. Oocytes were collected after superovulation, and their viability were compared using parthenogenetic activation. The aneuploidy of the oocytes (MII) was assessed using chromosome spread, and the whole ovarian follicle number was counted using an unbiased stereological method. Serum hormone levels were measured using the radio-immunity method, and the expression of the Cohesin subunit genes in the oocytes (GV) were assessed using RT-PCR. RESULTS:The mean number of recovered (25.8 vs. 16.2; P < 0.05) and live oocytes (24.0 vs. 11.73; P <0.01) per head in the young-mice group (6-week-old) was significantly higher than that of the aged group (9-month-old). The aneuploidy rate of the ovulated oocytes in the aged group was significantly higher than that of the young group (36.8% vs. 10%; P < 0.01), and the rate of blastocyst formation in the young group (85.23%) was significantly higher than that of the aged group (81.2%; P <0.05). The number of primordial follicles (the oocyte pool) per ovary in the aged group was significantly decreased compared with the young group (330 ± 33.51 vs. 2079.6 ± 420.70; P < 0.01), and the level of AMH in the aged group was significantly higher than that of the young group (4.66 ± 0.11 ng/ml vs. 4.07 ± 0.18 ng/ml; P < 0.01). CONCLUSIONS:We propose that maternal aging significantly reduces the oocyte pool, superovulation efficiency and developmental potential and increases the oocyte aneuploidy rate.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Fu X,Cheng J,Hou Y,Zhu Sdoi
10.1007/s10815-013-0160-5subject
Has Abstractpub_date
2014-03-01 00:00:00pages
323-31issue
3eissn
1058-0468issn
1573-7330journal_volume
31pub_type
杂志文章abstract：PURPOSE:Our purpose was to show that fertilization of oocytes can be obtained solely by laser light-mediated manipulation of gametes. METHOD:A small channel was drilled into the zona pellucida of bovine oocytes using an ultraviolet (UV)-laser microbeam. Highly diluted cattle sperm were not able to fertilize the laser ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02065947
更新日期：1996-03-01 00:00:00
abstract：PURPOSE:The results obtained with a protocol consisting of ovarian stimulation with low doses of highly purified FSH (FSH HP), administration of a GnRH analogue to induce an endogenous surge of gonadotropins, and IUI were evaluated. These results were compared with those seen with similar FSH stimulation and hCG admini...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1021127410300
更新日期：1997-10-01 00:00:00
abstract：PURPOSE:Previous studies reported that patients with endometriosis had excess nitric oxide (NO) in the reproductive tract and poor embryo development in IVF cycles. This study aims to elucidate the effects of NO on early embryo development. METHODS:Zygotes from superovulated B6CBF1 mice were cultured to blastocysts in...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0045-7
更新日期：2013-08-01 00:00:00
abstract：PURPOSE:Our purpose was to test whether age-related changes in antral follicle counts can predict the pregnancy outcome in the early follicular phase of a controlled ovarian hyperstimulation/intrauterine insemination (COH/IUI) program. METHODS:A selected group of 107 women (36 healthy women requesting child sex presel...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1022518103368
更新日期：1998-01-01 00:00:00
abstract：PURPOSE:Early timed follicular aspiration (ETFA) of one ovary 10-12 hr after administration of chorionic gonadotropin (hCG) is an attempt to prevent severe ovarian hyperstimulation syndrome (OHSS). After the introduction of early timed follicular aspiration ETFA of one ovary in IVF/ET cycles at high risk for severe OHS...
journal_title：Journal of assisted reproduction and genetics
pub_type： 临床试验,杂志文章
doi：10.1007/BF02070139
更新日期：1996-04-01 00:00:00
abstract：PURPOSE:The study aimed to investigate key intrafollicular prognostic factors among various cytokines and angiogenic molecules for prediction of mature oocytes and good-quality embryos in women with endometriosis undergoing in vitro fertilization (IVF). METHODS:Paired follicular fluid and serum samples were collected ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-016-0782-5
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue. METHODS:Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0105-z
更新日期：2013-11-01 00:00:00
abstract：BACKGROUND:Literature supports the efficacy of psychosocial intervention for infertile women. An eastern body-mind-spirit group intervention has been developed to help infertile women in the Chinese population cope with the distress arising from IVF treatment. METHODS:The eastern body-mind-spirit group intervention ad...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-005-6418-9
更新日期：2005-12-01 00:00:00
abstract：PURPOSE:Murine two-cell embryos (n = 5573) were cultured for 96 hr in human tubal fluid (HTF) medium (n = 2709) or alpha modification of minimum essential medium (MEM; n = 2864) through the hatched blastocyst stage from mid-1990 to mid-1991. An additional 373 embryos were cultured in MEM or HTF with 0, 1, 5, or 10 ng/m...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF01239220
更新日期：1993-04-01 00:00:00
abstract：PURPOSE:To evaluate the association between serum progesterone (P) levels on the day of embryo transfer (ET) and pregnancy rates in fresh donor IVF/ICSI cycles. METHODS:Fresh donor cycles with day 3 ET from 10/2007 to 8/2012 were included (n = 229). Most cycles (93 %) were programmed with a gonadotropin releasing horm...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0199-y
更新日期：2014-05-01 00:00:00
abstract：PURPOSE:Human reproduction is an inefficient process. There is a high rate of loss of early pregnancies, often before the mother (or physician) knows she is pregnant. Genetic abnormalities can explain much of the wastage, but can it explain all of the failures? As embryos bear paternal and embryonic antigens foreign to...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
doi：10.1023/a:1021788024214
更新日期：2003-02-01 00:00:00
abstract：:The improvement in life expectancy, following better cancer therapy combined with new options in treating male infertility have increased the use of sperm freezing. We describe a rare case of twin pregnancy of a childless widow, using donated oocytes after ICSI with her deceased husband's banked frozen spermatozoa. Sp...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1025485212589
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:This study measured platelet activating factor (PAF) production by rabbit embryos in vitro and ascertained if there is increased PAF production associated with advancing embryonic development. STUDY DESIGN:Two-cell rabbit embryos were recovered from superovulated New Zealand White does and cultured in vitro ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF01213432
更新日期：1993-07-01 00:00:00
abstract：PURPOSE:To compare morphokinetic parameters and quality of embryos derived from GnRH antagonist ICSI cycles triggered either with GnRH agonist or standard hCG between matched groups of patients. METHODS:Morphokinetic parameters of embryos derived from matched first GnRH antagonist ICSI cycles triggered by GnRH agonist...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01953-w
更新日期：2020-11-01 00:00:00
abstract：OBJECTIVE:The study aimed to investigate the impact of the peak E2 level during controlled ovarian hyperstimulation (COS) on the cumulative live birth rate (cLBR) in non-PCOS women with normal ovarian reserve. MATERIALS AND METHODS:Women between 20 and 39 years were included. Donor cycles and patients who never experi...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01568-w
更新日期：2019-11-01 00:00:00
abstract：PURPOSE:To evaluate the impact of early rescue ICSI on neonatal outcome. METHODS:This retrospective study compared the neonatal outcome of early rescue ICSI and ICSI with ejaculated sperm, including 233 children who were conceived after early rescue ICSI and a control group of 906 children who were conceived after ICS...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0245-9
更新日期：2014-07-01 00:00:00
abstract：PURPOSE:The importance of the surrounding ovarian stromal cells and extracellular matrix in the development and maturation of follicles has recently gained attention. An aberrant extracellular matrix has been described in ovaries of patients with polycystic ovary syndrome where a more rigid structural environment, poss...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01513-x
更新日期：2019-09-01 00:00:00
abstract：PURPOSE:Our purpose was to compare the diagnostic efficacy of laparoscopy between primary and secondary infertility and to define a subgroup in which the positive findings are low. METHODS:A retrospective review of the cases of 206 infertile women who underwent laparoscopy was conducted. RESULTS:Eighty-two (39.8%) pa...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1022578019260
更新日期：1998-10-01 00:00:00
abstract：PURPOSE:To investigate the impact of late follicular phase serum estradiol (E2) levels on implantation and pregnancy outcomes of cleavage stage cryopreserved/thawed embryos transferred in programmed cycles with exogenous hormonal replacement. METHODS:Retrospective cohort analysis of IVF patients with transfer of cryop...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0402-1
更新日期：2015-03-01 00:00:00
abstract：PURPOSE:To compare national trends and perinatal outcomes following the use of ejaculated versus surgically acquired sperm among IVF cycles with male factor infertility. METHODS:This retrospective cohort includes US fertility clinics reporting to the National ART Surveillance System between 2004 and 2015. Fresh, non-d...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-018-1178-5
更新日期：2018-07-01 00:00:00
abstract：PURPOSE:Retinal pigment epithelium cells derived from human embryonic stem cells (ESCs) could be useful for restoring retinal function in age-related macular degeneration. However the use of non-human feeder cells to support the growth of ESCs for clinical applications raises the concern of possible contamination becau...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-012-9802-2
更新日期：2012-08-01 00:00:00
abstract：PURPOSE:To investigate whether unexplained infertility at a young age demonstrates manifestations of decreased ovarian reserve. METHODS:A total of 100 women were divided into two equally sized groups. The study group comprised women aged ≤ 37 years diagnosed with unexplained infertility, and the control group included...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01467-0
更新日期：2019-06-01 00:00:00
abstract：PURPOSE:Artificial oocyte activation using calcium ionophores and enhancement of embryonic developmental potential by the granulocyte-macrophage colony-stimulating factor (GM-CSF) have already been reported. In this study, we evaluated the synergistic effect of these two methods on aged human unfertilized oocytes after...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-016-0823-0
更新日期：2017-01-01 00:00:00
abstract：PURPOSE:The purpose of this study is to determine if baseline antral follicle assessment may serve as additional information in predicting in vitro fertilization outcome. METHODS:Prospective, descriptive preliminary study of in vitro fertilization outcome. From July 1998 to July 1999, 224 patients underwent antral fol...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1009424407082
更新日期：2001-03-01 00:00:00
abstract：PURPOSE:To investigate the influence of sperm DNA integrity on the zona binding ability of mouse spermatozoa in relation to their sex chromosomal constitution. METHOD(S):In this prospective experimental study, the sperm DNA fragmentation was induced by exposing testicular area of Swiss Albino mice (Mus musculus) to di...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0123-x
更新日期：2013-12-01 00:00:00
abstract：PURPOSE:To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers. METHODS:Two-dimensional electrophoresis (2DE) followed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) were applied to es...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-011-9602-0
更新日期：2011-09-01 00:00:00
abstract：:Achieving successful somatic cell nuclear transfer (SCNT) in the human and subhuman primate relative to other mammals has been questioned for a variety of technical and logistical issues. Here we summarize the gradual evolution of SCNT technology from the perspective of oocyte quality and cell cycle status that has re...
journal_title：Journal of assisted reproduction and genetics
pub_type： 历史文章,杂志文章,评审
doi：10.1007/s10815-013-0053-7
更新日期：2013-08-01 00:00:00
abstract：PURPOSE:The purpose of this study was to determine the effect of stimulated and artificial endometrial preparation protocols on reproductive outcomes in frozen embryo transfer (FET) cycles. METHODS:We performed a retrospective study of 1926 FET cycles over a 3.5-year period in the Fertility Unit at a University Hospit...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-017-1078-0
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:The aim was to evaluate the effect of aspirin on pregnancy and implantation rates in an unselected group of patients undergoing intracytoplasmic sperm injection (ICSI). METHODS:Two hundred and seventy-nine patients were randomized to receive 80 mg of aspirin (n = 139) or no treatment (r = 136) starting from th...
journal_title：Journal of assisted reproduction and genetics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1023/a:1026491426423
更新日期：2000-11-01 00:00:00
abstract：:Artificial intelligence (AI) has been proposed as a potential tool to help address many of the existing problems related with empirical or subjective assessments of clinical and embryological decision points during the treatment of infertility. AI technologies are reviewed and potential areas of implementation of algo...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01950-z
更新日期：2020-11-01 00:00:00
