Abstract:
:Distribution of actin and fodrin, a nonerythroid analogue of spectrin, was studied in cytocentrifuge preparations and in tissue sections of normal and pathologic respiratory epithelium by using immunofluorescence and immunoelectron microscopy. In ciliated epithelial cells and in goblet cells of normal bronchial epithelium, fodrin and actin were located in the apical parts and along the lateral walls of the cells. In basal cells, fodrin and actin were also seen diffusely in the cytoplasm. Immunoelectron microscopy showed fodrin in close association with the basal bodies and rootlets of the cilia and microvilli in the ciliated cells. In alveolar epithelium, fodrin and actin were located at the apical membrane in type I pneumocytes and along the apical and lateral membranes in type II pneumocytes. In type II pneumocytes, fodrin was also seen in close association with the secretory vacuoles. In metaplastic and dysplastic bronchial epithelium, a diffuse cytoplasmic and a circumferential, membrane-associated staining for fodrin and actin was seen. In all types of carcinomas, fodrin was seen along the lateral walls and diffusely in the cytoplasm. The staining was more intense than in the normal cells. In immunoblotting of the normal bronchial epithelium, and peripheral lung and lung carcinomas, a single 240 kD band was recognized with antibodies to fodrin. The results show distinct differences in the distribution of fodrin in the various cell types of the respiratory epithelium. In ciliated cells, the close relationship with cytoskeleton suggests a role of fodrin in the establishment of the elaborate structural architecture of the apical compartment. In type II pneumocytes, on the other hand, fodrin probably plays a role in secretion of the surfactant. In basal cells, the diffuse distribution of fodrin probably reflects the high proliferative capacity of this cell compartment. Interestingly, a similar distribution was also seen in premalignant and malignant cells.
journal_name
Am J Respir Cell Mol Biolauthors
Sormunen R,Pääkkö P,Palovuori R,Soini Y,Lehto VPdoi
10.1165/ajrcmb.11.1.8018340subject
Has Abstractpub_date
1994-07-01 00:00:00pages
75-84issue
1eissn
1044-1549issn
1535-4989journal_volume
11pub_type
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journal_title:American journal of respiratory cell and molecular biology
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journal_title:American journal of respiratory cell and molecular biology
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journal_title:American journal of respiratory cell and molecular biology
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更新日期:2011-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1165/rcmb.2016-0118OC
更新日期:2017-05-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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doi:10.1165/rcmb.2007-0004RC
更新日期:2007-07-01 00:00:00
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更新日期:2009-06-01 00:00:00
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更新日期:2013-02-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.20.6.3455
更新日期:1999-06-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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更新日期:2011-07-01 00:00:00
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