Embryo cleavage rates after oocyte insemination with capacitated sperms in the presence or absence of follicular fluid.

Abstract:

:The objective of the present study was to compare the rate of embryo cleavage after oocyte insemination with capacitated spermatozoa in the presence or absence of follicular fluid. In the first experiment, a total of 67 oocytes were inseminated with spermatozoa which had been submitted to part of the capacitation procedures in the presence of follicular fluid (21 oocytes) and with spermatozoa capacitated only with Menezo B2 culture medium (46 oocytes). Thirteen and 29 cleaved embryos were obtained, respectively (p = 0.89). In the second experiment, a total of 61 oocytes were inseminated with spermatozoa submitted to the entire capacitation procedure in follicular fluid (17 oocytes) and capacitated only with Menezo B2 culture medium (44 oocytes). Eleven and 31 cleaved embryos were obtained, respectively (p = 0.67). The absence of significant differences in cleavage rate between the two experiments indicates that the quality of the spermatozoa capacitation process is not changed by the addition of follicular fluid.

journal_name

Gynecol Obstet Invest

authors

Franco Júnior JG,Mauri AL,Petersen CG,Baruffi RL,Campos MS,Oliveira JB

doi

10.1159/000292545

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

145-9

issue

3

eissn

0378-7346

issn

1423-002X

journal_volume

37

pub_type

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