Abstract:
:Recent electrophysiological investigations in this laboratory have shown that cultured mesopontine serotonergic neurons from neonatal rats evoke serotonergic and/or glutamatergic responses in themselves and in non-serotonergic neurons. Serotonergic nerve terminals in vivo are heterogeneous with respect to vesicle type, synaptic structure, and the frequency with which they form conventional synaptic contacts, but the functional correlates of this heterogeneity are unclear. We have therefore examined the ultrastructure of electrophysiologically-characterized synapses formed by cultured serotonergic neurons, and have compared the findings with the ultrastructural characteristics of serotonergic synapses reported in vivo. Dissociated rat serotonergic neurons in microcultures were identified by serotonin immunocytochemistry or by uptake of the autofluorescent serotonin analogue 5,7-dihydroxytryptamine, and were subsequently processed for electron microscopy. Unlabeled axon terminals formed numerous synapses on serotonin-immunoreactive somata and dendrites. Serotonin-immunoreactive axon terminals formed synapses on the somata, dendrites and somatodendritic spine-like appendages of serotonergic and non-serotonergic neurons. In microcultures containing a solitary serotonergic neuron that evoked glutamatergic or serotonergic/glutamatergic autaptic responses, both symmetric and asymmetric synapses were present. In addition to large dense core vesicles, individual neurons contained either microcanaliculi and microvesicles, clear round vesicles, or clear pleiomorphic vesicles. For a given cell, however, the subtypes of vesicles present in each axon terminal were similar. Thus, dissociated serotonergic and non-serotonergic raphe neurons formed functional, morphological synapses in culture. A direct examination of both the synaptic physiology and ultrastructure of single cultured serotonergic neurons indicated that these cells released serotonin and glutamate at synapses that were morphologically similar to synapses formed by serotonergic neurons in vivo. The findings also suggested that individual serotonergic neurons differ with respect to synaptic vesicle morphology, and are capable of simultaneously forming symmetric and asymmetric synapses with target cells.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Johnson MD,Yee AGdoi
10.1016/0306-4522(95)00010-gsubject
Has Abstractpub_date
1995-08-01 00:00:00pages
609-23issue
3eissn
0306-4522issn
1873-7544pii
0306-4522(95)00010-Gjournal_volume
67pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The cellular localization of preprosomatostatin mRNA in the rat brain and sensory ganglia has been examined in detail using a newly developed highly sensitive non-radioactive in situ hybridization histochemistry procedure. An alkaline phosphatase labelled anti-sense 30mer oligodeoxynucleotide probe was used for detect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90388-k
更新日期:1990-01-01 00:00:00
abstract::Neurons of the efferent vestibular system were investigated in the cat using retrograde axonal transport of horseradish peroxidase and fluorescent retrograde double labelling techniques. The number of efferent neurons was clearly higher than previously reported. A three dimensional reconstruction of the location of th...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90200-8
更新日期:1984-04-01 00:00:00
abstract::The neuropeptides somatostatin, neurotensin and substance P were investigated in rats during and after limbic seizures induced by systemic injection of kainic acid (10 mg/kg, i.p.). Three hours after injection of the toxin, pronounced decreases (40-50%) in somatostatin-like immunoreactivity in frontal cortex, striatum...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90081-3
更新日期:1986-04-01 00:00:00
abstract::Pathological narcissism is often characterized by arrogant behavior, a lack of empathy, and willingness to exploit other individuals. Generally, individuals with high levels of narcissism are more likely to suffer mental disorders. However, the brain structural basis of individual pathological narcissism trait among h...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.04.025
更新日期:2016-07-22 00:00:00
abstract::Subcutaneous or intracerebroventricular injection of either arginine8-vasopressin or desglycinamide9-arginine8-vasopressin has been shown to facilitate memory, reduce or reverse the effects of amnesic drugs, and maintain tolerance to some effects of ethanol. These actions of vasopressin (and, by inference, of desglyci...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00709-x
更新日期:1999-01-01 00:00:00
abstract::In this study, we describe a model of opiate sensitization characterized by a brief schedule of treatment with repeated morphine administrations. In this model, we investigated the changes produced by repeated morphine treatment on dopamine transmission at the level of the two major terminal dopaminergic areas, the do...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00466-7
更新日期:1999-05-01 00:00:00
abstract::The hippocampus plays an important role in the formation of new memories and spatial navigation. Recently, growing evidence supports the view that it is also involved in addiction to opiates and other drugs. Theoretical and experimental studies suggest that hippocampal neural-network oscillations at specific frequenci...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.09.052
更新日期:2010-12-15 00:00:00
abstract::Allotetrahydrodeoxycorticosterone (THDOC) belongs to a class of pregnane neurosteroidal compounds that enhance brain inhibition by interacting directly with GABAA signaling, mainly through an increase in tonic inhibitory current. Here, we addressed the role of THDOC in the modulation of interictal- and ictal-like acti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.10.025
更新日期:2014-01-03 00:00:00
abstract::I.c.v. injection of L-ornithine has been shown to have sedative and hypnotic effects on neonatal chicks exposed to acute stressful conditions. To clarify the mechanism, we conducted three experiments under strengthened stressful conditions with corticotropin-releasing factor (CRF). In Experiment 1, the effect of i.c.v...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.10.076
更新日期:2011-01-13 00:00:00
abstract::Organophosphates (OPs) pose a constant threat to human health due to their widespread use as pesticides and their potential employment in military and terrorist attacks. The acute toxicity of OPs has been extensively studied; however, the consequences of prolonged or repeated exposure to levels of OPs that produce no ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.12.031
更新日期:2011-03-10 00:00:00
abstract::The rodent whisker-barrel system is characterized by its patterned somatotopic mapping between the sensory periphery and multiple regions of the brain. While somatotopy in the whisker system is established, we know far less about how preferences for stimulus orientation or other features are organized. Mouse somatosen...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.07.067
更新日期:2018-01-01 00:00:00
abstract::Transcranial magnetic stimulation (TMS) has opened important perspectives on the pathophysiological bases and potential targets of treatment strategies for idiopathic Parkinson's disease (IPD). Studies have been mainly focusing on motor cortical inhibitory phenomena. However, differences in patients and methods caused...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.08.033
更新日期:2007-11-30 00:00:00
abstract::An original pathway of centrifugal acetylcholinesterase-positive fibres is described in the olfactory bulb of the rat. A dense network of positive fibres spreads out superficially at the boundaries of the lateral olfactory tract and the glomerular layer. These labelled fibres converge towards atypical glomerular struc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90183-7
更新日期:1987-12-01 00:00:00
abstract::In partially denervated skeletal muscle, spared motor fibres sprout, enlarging motor unit size. Neuritogenesis and sprouting are known to depend on the synaptic input to the neurons. This suggests that spared motoneuron reaction to partial muscle denervation might be controlled by primary sensory neurons which directl...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00506-x
更新日期:1998-05-01 00:00:00
abstract::Correlated pre- and postsynaptic activity is thought to promote maturation of excitatory synapses in the developing brain by directing AMPA receptors to pure NMDA synapses. However, this hypothesis has not been tested in vivo. Here, we have performed such test by inhibiting correlated neural activity in vivo using a s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00366-x
更新日期:2003-01-01 00:00:00
abstract::The globus pallidus sends a significant GABAergic projection to the thalamic reticular nucleus. Because pallidal neurons express D4-dopamine receptors, we have explored their presence on pallidoreticular terminals by studying the effect of dopamine and D4-receptor agonists on the GABAergic transmission in the thalamic...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.05.048
更新日期:2010-09-15 00:00:00
abstract::Social play is a motivated and rewarding behavior that is displayed by nearly all mammals and peaks in the juvenile period. Moreover, social play is essential for the development of social skills and is impaired in social disorders like autism. We recently showed that the lateral septum (LS) is involved in the regulat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.08.052
更新日期:2015-10-29 00:00:00
abstract::l-3,4-Dihydroxyphenylalanine (l-DOPA) is the therapeutic gold standard in Parkinson's disease. However, most patients develop debilitating abnormal involuntary movements termed l-DOPA-induced dyskinesia (LID) as therapy-complicating side effects. The underlying mechanisms of LID pathogenesis are still not fully unders...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.06.017
更新日期:2016-09-07 00:00:00
abstract::Chronic administration of mu-opioid receptor agonists is known to produce adaptive changes within noradrenergic neurons of the locus coeruleus. Although mu-opioid receptors are densely expressed by locus coeruleus neurons, the effects of acute and chronic administration of agonists on the subcellular distribution of m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00426-2
更新日期:2001-01-01 00:00:00
abstract::An increase of extracellular dopamine (DA) has been implicated in the psychostimulant properties of 3,4-methylenedioxymethamphetamine (MDMA). Although this drug has been reported to affect the DA uptake transporter (DAT), it might activate other mechanisms to regulate the outflow of DA in the brain. Our aim was to exa...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.02.037
更新日期:2018-05-01 00:00:00
abstract::In the adult CNS, tissue-specific germinal niches, such as the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus of the hippocampus, contain multipotent neural precursor cells (NPCs) with the capacity to self-renew and differentiate into functional brain cells (i.e. neurons, a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.07.022
更新日期:2013-10-10 00:00:00
abstract::The transition from multipotent neural stem cells (NSCs) to terminally differentiated neurons is a multistep process, and the transition is finely regulated by transcription factors with basic helix-loop-helix (bHLH) motifs. Melatonin is an endogenous neurohormone with profound neurotrophic and neuroprotective effects...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.07.064
更新日期:2017-11-19 00:00:00
abstract::The aim of the present study was to identify potential biomarkers for depression in the search for novel disease targets and treatment regimens. Furthermore, the study includes a search for biomarkers involved in treatment resistance and stress resilience in order to investigate mechanisms underlying antidepressant dr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.08.024
更新日期:2011-11-24 00:00:00
abstract::The central part of the retina, which includes the fovea, is substantially overrepresented in the topographic map of the retina in the striate cortex. We tested whether this simply reflects the uneven distribution of ganglion cells in the retina in accordance with the "principle" of peripheral scaling, or whether ther...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00589-7
更新日期:1996-06-01 00:00:00
abstract::Synapsins are a family of synaptic vesicle (SV)-associated phosphoproteins that have been identified in several vertebrates and invertebrates. We report here the cloning and expression of synapsin family genes in the zebrafish Danio rerio. We identified the complete coding sequence of synapsin 3, which is not present ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.09.015
更新日期:2014-11-07 00:00:00
abstract::The hypocretin/orexin system regulates, among other things, sleep and energy homeostasis. The system is likely regulated by both homeostatic and circadian mechanisms. Little is known about local differences in the regulation of hypocretin activity. The aim of this study was to establish an optimized peptide quantifica...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.09.050
更新日期:2015-12-03 00:00:00
abstract::The distribution of several neuropeptides (vasoactive intestinal polypeptide, cholecystokinin octapeptide, substance P, neurotensin, methionine-enkephalin and somatostatin) in the hippocampal formation has been studied with immunocytochemical techniques. Numerous vasoactive intestinal polypeptide, cholecystokinin-octa...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90214-8
更新日期:1984-01-01 00:00:00
abstract::Recent evidence suggests that ischemia/reperfusion (I/R) in an organ may have distance effect on the brain. In this study, the effects of renal I/R, limb I/R or both together on the structural and function of hippocampus were evaluated and compared. Hence, rats were subjected to 2-h bilateral lower limb ischemia, 45-m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.06.058
更新日期:2017-09-01 00:00:00
abstract::Studies in rats and mice have shown several sex-dependent functional and structural differences in the hippocampal region, a brain structure playing a key role in learning and memory. The aim of the present study was to establish whether sex differences exist prior to puberty in the stereological parameters of the den...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.12.015
更新日期:2005-01-01 00:00:00
abstract::Vascular endothelial cells were activated during acute ischemic brain injury, which could induce neural progenitor cell proliferation and migration. However, the mechanism was still unknown. In the current study, we explored whether vascular endothelial cells promoted neural progenitor cell proliferation and whether m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.05.046
更新日期:2020-08-10 00:00:00