Abstract:
:This article focusses on the structural development of human esophageal ciliated epithelium. A combination of transmission electron microscopic (TEM), scanning electron microscopic (SEM), radioautographic, and light microscopic (LM) analyses were carried out using intact fetal tissues between 8 and 20 weeks of gestation as well as cultured esophageal explants. Up to the age of 10 weeks, the stratified esophageal epithelium consisted of two longitudinal primary folds. The surface cells were undifferentiated and contained large glycogen aggregates. Between 11 and 16 weeks, the primary folds (now up to four) had developed secondary folds. The thickness of the epithelium drastically increased (123%) in concomittance with a differentiation of surface columnar ciliated cells. These highly specialized surface cells exhibited junctional complexes and well-developed organelles with numerous microvilli interspersed among the cilia. Transverse sections revealed the internal structure of the cilia with a consistent pattern of nine doublet microtubules surrounding a central pair of single microtubules. Freeze-fracture studies illustrated the presence of a ciliary necklace composed of 6 ring-like rows of intramembranous particles. They also revealed the structure of ciliary cell tight junctions consisting of up to nine anastomosing strands (P-face) or complementary grooves (E-face). Ultrastructural studies (LM, TEM, SEM) of the esophageal squamous epithelium obtained after 15 days of culture showed that the newly formed epithelium was similar to adult human epithelium. Finally LM and SEM observations established that the esophagogastric junction was not yet well delineated, consisting of a transitional area composed of a mixture of esophageal ciliated cells and gastric columnar mucous cells.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Ménard Ddoi
10.1002/jemt.1070310305subject
Has Abstractpub_date
1995-06-15 00:00:00pages
215-25issue
3eissn
1059-910Xissn
1097-0029journal_volume
31pub_type
杂志文章abstract::The metrial gland develops in the uterus of many rodent species during normal pregnancy. It is a maternally-derived tissue that contains stromal and vascular elements plus a population of large cells, striking in their light microscopic appearance due to the presence of numerous cytoplasmic granules. These cells, whic...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070250302
更新日期:1993-06-15 00:00:00
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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