Abstract:
:Red cell adenosine deaminase from normal subjects and from a patient with hereditary hemolytic anemia with a 40-fold increase in activity were purified using antibody affinity chromatography. The purified enzymes were completely homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. There were no differences in the molecular weight, specific activity, polyacrylamide gel electrophoresis, Michaelis constant for adenosine, inhibition constant for guanylurea, utilization of 2-deoxyadenosine, thermal stability, optimum pH, immunological reactivity, amino acid composition and tryptic peptide mapping. These results strongly suggest that increased red cell adenosine deaminase activity is caused by an overproduction of a structurally normal enzyme.
journal_name
Hemoglobinjournal_title
Hemoglobinauthors
Fujii H,Miwa S,Suzuki Kdoi
10.3109/03630268008997738subject
Has Abstractpub_date
1980-01-01 00:00:00pages
693-705issue
5-6eissn
0363-0269issn
1532-432Xjournal_volume
4pub_type
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