Effect of ischemia on the activity of DNA-dependent RNA polymerase and DNA polymerase.

abstract：:We previously reported that nerve terminals and glial cells lack an active uptake system capable of terminating transmitter action of substance P (SP). In the present study, we demonstrated the existence of an active uptake system for SP carboxy-terminal heptapeptide, (5-11)SP. When the slices from either rat brain or...

journal_title：Journal of neurochemistry

authors： Nakata Y,Kusaka Y,Yajima H,Segawa T

更新日期：1981-12-01 00:00:00

abstract：:Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain scaffolding protein with kinase and GTPase activities involved in synaptic vesicle (SV) dynamics. While its role in Parkinson's disease has been largely investigated, little is known about LRRK2 physiological role and until now few proteins have been describe...

journal_title：Journal of neurochemistry

authors： Marte A,Russo I,Rebosio C,Valente P,Belluzzi E,Pischedda F,Montani C,Lavarello C,Petretto A,Fedele E,Baldelli P,Benfenati F,Piccoli G,Greggio E,Onofri F

更新日期：2019-08-01 00:00:00

abstract：:In NG 108-15 clonal cells, extracellular application of micromolar concentrations of serotonin [5-hydroxytryptamine (5-HT)] and substance P induces the opening of a cation permeability monitored by the influx of [14C]-guanidinium. The serotoninergic component of this cation permeability is linked to 5-HT3 receptor act...

journal_title：Journal of neurochemistry

authors： Riad M,Hamon M,Emerit MB

更新日期：1994-06-01 00:00:00

abstract：:The neurotoxic effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was tested on mice lacking the dopamine (DA) transporter (DAT-/- mice). Striatal tissue DA content and glial fibrillary acidic protein (GFAP) mRNA expression were assessed as markers of MPTP neurotoxicity. MPTP (30 mg/kg, s.c., b.i.d.) produc...

journal_title：Journal of neurochemistry

authors： Gainetdinov RR,Fumagalli F,Jones SR,Caron MG

更新日期：1997-09-01 00:00:00

abstract：:We assessed the functional expression of the norepinephrine (NE) transporter (NET) in cultured rat cortical astrocytes. Specific [3H]NE uptake increased in a time-dependent manner, and this uptake involves temperature- and Na+-sensitive mechanisms. The Na+-dependent [3H]NE uptake was saturable, and the Km for the proc...

journal_title：Journal of neurochemistry

authors： Inazu M,Takeda H,Matsumiya T

更新日期：2003-01-01 00:00:00

abstract：:Previous studies on central 5-hydroxytryptamine1A (5-HT1A) receptors have consistently shown the existence of a GTP-insensitive component of agonist binding, i.e., binding of [3H]8-hydroxy-2-(di-n-propylamino)tetralin ([3H]8-OH-DPAT) that persists in the presence of 0.1 mM GTP or guanylylimidodiphosphate (GppNHp). The...

journal_title：Journal of neurochemistry

authors： Emerit MB,Miquel MC,Gozlan H,Hamon M

更新日期：1991-05-01 00:00:00

abstract：:Sequences in the transcribed region of the c-fos gene have been suggested to control c-fos induction following exposure of cells to mitogens or stimuli that increase intracellular calcium concentrations. Using a mutational analysis we show that putative regulatory elements present in the first intron of the human c-fo...

journal_title：Journal of neurochemistry

authors： Lange CM,Bading H

更新日期：2001-06-01 00:00:00

abstract：:Tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of the catecholamines dopamine, noradrenaline and adrenaline, is regulated acutely by feedback inhibition by the catecholamines and relief of this inhibition by phosphorylation of serine 40 (Ser40). Phosphorylation of serine 40 abolishes the bindi...

journal_title：Journal of neurochemistry

authors： Gordon SL,Quinsey NS,Dunkley PR,Dickson PW

更新日期：2008-08-01 00:00:00

abstract：:Benzodiazepine receptor binding was measured in cerebellar cortex of 15 patients with dominantly inherited olivopontocerebellar atrophy (OPCA). The majority of these patients had a moderate to marked Purkinje cell loss, as judged by the lowered levels of dentate nucleus gamma-aminobutyric acid (GABA), a marker of Purk...

journal_title：Journal of neurochemistry

authors： Kish SJ,Perry TL,Hornykiewicz O

更新日期：1984-02-01 00:00:00

abstract：:Primary astrocyte cultures from neonatal rat brains show uptake of [3H]norepinephrine ([3H]NE). This uptake has a high-affinity component with an apparent Km of approximately 3 X 10(-7) M. At 10(-7) M [3H]NE both the initial rate of uptake and steady-state content of [3H]NE is inhibited by up to 95% by omission of ext...

journal_title：Journal of neurochemistry

authors： Kimelberg HK,Pelton EW 2nd

更新日期：1983-05-01 00:00:00

abstract：:This article reports the results of a systematic investigation of the different types of antibodies produced in the course of a long-term immunization of rats with mouse nerve growth factor (NGF). We have characterized three types of monoclonal antibodies, namely: (1) antibodies that bind to NGF and inhibit its bindin...

journal_title：Journal of neurochemistry

authors： Cattaneo A,Rapposelli B,Calissano P

更新日期：1988-04-01 00:00:00

abstract：:Conditioned medium derived from a rat central nervous system neuronal cell line B104 (B104 CM) was shown previously to contain uncharacterized potent mitogen(s) for oligodendrocyte/type-2 astrocyte (O-2A) progenitor cells. In this study, we demonstrated that B104 cells produce and secrete platelet-derived growth facto...

journal_title：Journal of neurochemistry

authors： Asakura K,Hunter SF,Rodriguez M

更新日期：1997-06-01 00:00:00

abstract：:Our laboratory demonstrated previously that PGE2-induced modulation of hippocampal synaptic transmission is via a pre-synaptic PGE2 EP2 receptor. However, little is known about whether the EP2 receptor is involved in hippocampal long-term synaptic plasticity and cognitive function. Here we show that long-term potentia...

journal_title：Journal of neurochemistry

authors： Yang H,Zhang J,Breyer RM,Chen C

更新日期：2009-01-01 00:00:00

abstract：:Prolonged seizures activate members of the Bcl-2 homology domain 3-only sub-group of the Bcl-2 protein family, which are essential for initiation of apoptosis signaling. Bid is a potent pro-apoptotic Bcl-2 homology domain 3-only protein, which upon proteolytic activation translocates to mitochondria to promote activat...

journal_title：Journal of neurochemistry

authors： Engel T,Caballero-Caballero A,Schindler CK,Plesnila N,Strasser A,Prehn JH,Henshall DC

更新日期：2010-10-01 00:00:00

abstract：:The binding of L-2-[3H]amino-4-phosphonobutyrate ([3H]L-AP4) was examined in brain sections of wild-type mice and mice lacking the mGluR4 subtype of metabotropic glutamate receptors (mGluRs). Very high relative densities of [3H]L-AP4 binding were observed in the molecular layer of the cerebellar cortex, the nucleus ba...

journal_title：Journal of neurochemistry

authors： Thomsen C,Hampson DR

更新日期：1999-02-01 00:00:00

abstract：:Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9...

journal_title：Journal of neurochemistry

authors： Roeder LM,Williams IB,Tildon JT

更新日期：1985-11-01 00:00:00

abstract：:L-glutamate (Glu) is the predominant excitatory neurotransmitter in the mammalian central nervous system. It plays major roles in normal neurophysiology and many brain disorders by binding to membrane-bound Glu receptors. To overcome the spatial and temporal limitations encountered in previous in vivo extracellular Gl...

journal_title：Journal of neurochemistry

authors： Day BK,Pomerleau F,Burmeister JJ,Huettl P,Gerhardt GA

更新日期：2006-03-01 00:00:00

abstract：:Cystatin C, a well-established biomarker of renal function, has been associated with a protective effect against stroke. However, the potential neuroprotective mechanism of cystatin C in ischemic brain injury remains unclear. Our study hypothesized that cystatin C can ameliorate blood-brain barrier (BBB) disruption by...

journal_title：Journal of neurochemistry

authors： Yang B,Xu J,Chang L,Miao Z,Heang D,Pu Y,Zhou X,Zhang L,Xie H

更新日期：2020-05-01 00:00:00

abstract：:Transcriptional dysregulation is a hallmark of Huntington's disease (HD) and one cause of this dysregulation is enhanced activity of the REST-mSIN3a-mSIN3b-CoREST-HDAC repressor complex, which silences transcription through REST binding to the RE1/NRSE silencer. Normally, huntingtin (HTT) prevents this binding, allowi...

journal_title：Journal of neurochemistry

authors： Conforti P,Zuccato C,Gaudenzi G,Ieraci A,Camnasio S,Buckley NJ,Mutti C,Cotelli F,Contini A,Cattaneo E

更新日期：2013-10-01 00:00:00

abstract：:Methylone, 3,4-methylenedioxypyrovalerone (MDPV), and mephedrone are psychoactive ingredients of 'bath salts' and their abuse represents a growing public health care concern. These drugs are cathinone derivatives and are classified chemically as β-ketoamphetamines. Because of their close structural similarity to the a...

journal_title：Journal of neurochemistry

authors： Anneken JH,Angoa-Pérez M,Kuhn DM

更新日期：2015-04-01 00:00:00

abstract：:The thalamic synapses relay peripheral sensory information to the cortex, and constitute an important part of the thalamocortical network that generates oscillatory activities responsible for different vigilance (sleep and wakefulness) states. However, the modulation of thalamic synaptic transmission by potential slee...

journal_title：Journal of neurochemistry

authors： Yang YC,Hu CC,Huang CS,Chou PY

更新日期：2014-03-01 00:00:00

abstract：:We describe here two types of apoptotic cell death observed in the rat CNS-derived neuroblastoma B50 and B104 cells. One type was induced by dibutyryl cyclic AMP (DBcAMP) after differentiation, and the other was induced by treatment of proliferating cells with cycloheximide. When B50 and B104 cells were treated with 1...

journal_title：Journal of neurochemistry

authors： Honma N,Uchida A,Hirose H,Srsen V,Kishimoto T,Hisanaga S

更新日期：1996-11-01 00:00:00

abstract：:We used both the polymerase chain reaction (PCR) and in situ hybridization to search for the presence of proteolipid protein (PLP) gene transcripts in the developing mouse. Total brain RNA extracted from 13-19-day embryos, analyzed by PCR, demonstrated the presence of a single transcript that was unambiguously identif...

journal_title：Journal of neurochemistry

authors： Timsit SG,Bally-Cuif L,Colman DR,Zalc B

更新日期：1992-03-01 00:00:00

abstract：:Staufen is an RNA-binding protein, first identified for its role in oogenesis and CNS development in Drosophila. Two mammalian homologs of Staufen have been identified and shown to bind double-stranded RNA and tubulin, and to function in the somatodendritic transport of mRNA in neurons. Here, we examined whether Stauf...

journal_title：Journal of neurochemistry

authors： Bélanger G,Stocksley MA,Vandromme M,Schaeffer L,Furic L,DesGroseillers L,Jasmin BJ

更新日期：2003-08-01 00:00:00

abstract：:The histamine H1 receptor binding capacity of the amygdalas of amygdaloid kindled rats was studied. In the kindled nonstimulated amygdala, significant decreases in K(D) and B(max) values compared with those of control amygdala were found 1 week after the last kindled seizure. One month after the last kindled seizure, ...

journal_title：Journal of neurochemistry

authors： Toyota H,Ito C,Yanai K,Sato M,Watanabe T

更新日期：1999-05-01 00:00:00

abstract：:Previous studies have shown that activation of the cyclic AMP (cAMP) pathway down-regulates CREB expression in CATH.a cells, an effect that appears to be mediated via inhibition of CREB gene transcription. In the current study, we compared this effect in CATH.a cells with regulation of CREB expression in another cell ...

journal_title：Journal of neurochemistry

authors： Coven E,Ni Y,Widnell KL,Chen J,Walker WH,Habener JF,Nestler EJ

更新日期：1998-11-01 00:00:00

abstract：:Primary cortical neurones exposed to an oxidative insult in the form of hydrogen peroxide (H(2)O(2)) for 30 min showed a concentration-dependent increase in oxidative stress followed by a delayed NMDA receptor-dependent cell death measured 24 h later. Extracellular signal-regulated protein kinase (ERK1/2), c-jun N-ter...

journal_title：Journal of neurochemistry

authors： Crossthwaite AJ,Hasan S,Williams RJ

更新日期：2002-01-01 00:00:00

abstract：:Complementary DNAs that encode two forms of the chicken gamma-aminobutyric acid type A (GABAA) receptor beta 2 subunit have been isolated. These polypeptides differ by the presence of (beta 2L) or absence (beta 2S) of 17 amino acids, which contain a possible target for phosphorylation by protein kinase C, in the large...

journal_title：Journal of neurochemistry

authors： Harvey RJ,Chinchetru MA,Darlison MG

更新日期：1994-01-01 00:00:00

abstract：:In rats rendered hyperthyroid by chronic treatment with L-triiodothyronine (T3) hormone there was a 21 and 27% decrease, respectively, in the number of binding sites for [3H]flunitrazepam ([3H]FNZ) and [3H]ethyl-beta-carboline-3-carboxylate ([3H]beta-CCE) without changes in affinity for the two ligands. Two weeks afte...

journal_title：Journal of neurochemistry

authors： Medina JH,De Robertis E

更新日期：1985-05-01 00:00:00

abstract：:Neurofilament proteins (Nf) are a biomarker of disease progression in amyotrophic lateral sclerosis (ALS). This study investigated whether there are major differences in expression from in vivo measurements of neurofilament isoforms, from the light chain, NfL (68 kDa), compared with larger proteins, the medium chain (...

journal_title：Journal of neurochemistry

authors： Zucchi E,Lu CH,Cho Y,Chang R,Adiutori R,Zubiri I,Ceroni M,Cereda C,Pansarasa O,Greensmith L,Malaspina A,Petzold A

更新日期：2018-09-01 00:00:00