Abstract：
:L-glutamate (Glu) is the predominant excitatory neurotransmitter in the mammalian central nervous system. It plays major roles in normal neurophysiology and many brain disorders by binding to membrane-bound Glu receptors. To overcome the spatial and temporal limitations encountered in previous in vivo extracellular Glu studies, we employed enzyme-coated microelectrode arrays to measure both basal and potassium-evoked release of Glu in the anesthetized rat brain. We also addressed the question of signal identity, which is the predominant criticism of these recording technologies. In vivo self-referencing recordings demonstrated that our Glu signals were both enzyme- and voltage-dependent, supporting the identity of L-glutamate. In addition, basal Glu was actively regulated, tetrodotoxin (TTX)-dependent, and measured in the low micromolar range (approximately 2 microm) using multiple self-referencing subtraction approaches for identification of Glu. Moreover, potassium-evoked Glu release exhibited fast kinetics that were concentration-dependent and reproducible. These data support the hypothesis that Glu release is highly regulated, requiring detection technologies that must be very close to the synapse and measure on a second-by-second basis to best characterize the dynamics of the Glu system.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Day BK,Pomerleau F,Burmeister JJ,Huettl P,Gerhardt GAdoi
10.1111/j.1471-4159.2006.03673.xkeywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1626-35issue
6eissn
0022-3042issn
1471-4159pii
JNC3673journal_volume
96pub_type
杂志文章abstract：:Huntington's disease (HD) is an autosomal-dominant neurodegenerative disorder caused by a poly-glutamine expansion in huntingtin, the protein encoded by the HD gene. PolyQ-expanded huntingtin is toxic to neurons, especially the medium spiny neurons of the striatum. At the same time, wild-type huntingtin has important ...
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更新日期：2007-06-01 00:00:00
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更新日期：1994-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1992.tb10980.x
更新日期：1992-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1984.tb12751.x
更新日期：1984-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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更新日期：2007-08-01 00:00:00
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更新日期：2005-03-01 00:00:00
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更新日期：2014-04-01 00:00:00
