Immunocytochemical localization of cellular retinol binding protein in the rat retina.

abstract：PURPOSE:Oxidative stress and metabolic dysregulation of the RPE have been implicated in AMD; however, the molecular regulation of RPE metabolism remains unclear. The transcriptional coactivator, peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) is a powerful mediator of mitochondrial function. Th...

journal_title：Investigative ophthalmology & visual science

authors： Iacovelli J,Rowe GC,Khadka A,Diaz-Aguilar D,Spencer C,Arany Z,Saint-Geniez M

更新日期：2016-03-01 00:00:00

abstract：PURPOSE:To investigate functional interactions between the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and the nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent antioxidant system in cultured human retinal pigment epithelium (RPE) cells. METHODS:Cultured ARPE-19 cells were treated with different concen...

journal_title：Investigative ophthalmology & visual science

authors： Wang L,Chen Y,Sternberg P,Cai J

更新日期：2008-04-01 00:00:00

abstract：PURPOSE:To explore connexin43 (Cx43) knockdown as an efficient treatment for corneal endothelial injury in an in vivo rat corneal scrape injury model. METHODS:Scrape injury was induced in the corneal endothelium, and immunolabeling (ZO-1, alpha-SMA, Cx43) was performed to analyze changes in Cx43 expression during woun...

journal_title：Investigative ophthalmology & visual science

authors： Nakano Y,Oyamada M,Dai P,Nakagami T,Kinoshita S,Takamatsu T

更新日期：2008-01-01 00:00:00

abstract：PURPOSE:RPE65 has been shown to be essential for the production of 11-cis retinal by the retinal pigment epithelium. Mutations in RPE65 are known to be associated with severe forms of early-onset retinal dystrophy. This project was designed to determine the amount of regenerable opsin in Rpe65-/- mice during developmen...

journal_title：Investigative ophthalmology & visual science

authors： Rohrer B,Goletz P,Znoiko S,Ablonczy Z,Ma JX,Redmond TM,Crouch RK

更新日期：2003-01-01 00:00:00

abstract：:The microvascular permeability response of the guinea pig conjunctiva to sulfidopeptide leukotrienes (LTs) was quantified as extravasation of radiolabeled bovine serum albumin. The LTs were potent inducers of increased microvascular permeability, with relative potencies LTE4 greater than or equal to LTD4 greater than ...

journal_title：Investigative ophthalmology & visual science

authors： Gary RK Jr,Woodward DF,Nieves AL,Williams LS,Gleason JG,Wasserman MA

更新日期：1988-01-01 00:00:00

abstract：PURPOSE:Defensins are naturally occurring antimicrobial peptides. Recently the authors published evidence of defensin production by the human ocular surface. A study was undertaken to look for intraocular defensins that may account for unexplained antimicrobial activity of intraocular fluids. METHODS:Reverse transcrip...

journal_title：Investigative ophthalmology & visual science

authors： Haynes RJ,McElveen JE,Dua HS,Tighe PJ,Liversidge J

更新日期：2000-09-01 00:00:00

abstract：Purpose:To determine whether mitochondrial DNA haplogroups or rare variants associate with primary open-angle glaucoma in subjects of European descent. Methods:A case-control comparison of age- and sex-matched cohorts of 90 primary open-angle glaucoma patients and 95 population controls. Full mitochondrial DNA sequenc...

journal_title：Investigative ophthalmology & visual science

authors： Singh LN,Crowston JG,Lopez Sanchez MIG,Van Bergen NJ,Kearns LS,Hewitt AW,Yazar S,Mackey DA,Wallace DC,Trounce IA

更新日期：2018-09-04 00:00:00

abstract：:The authors compared cytoskeletal elements of the in situ human trabecular-meshwork cell with in situ human corneal cells using indirect immunofluorescence staining for tubulin and intermediate filaments (vimentin, cytokeratin, and desmin) and NBD-phallacidin staining for f-actin using both fixed frozen and unfixed fr...

journal_title：Investigative ophthalmology & visual science

authors： Weinreb RN,Ryder MI

更新日期：1990-09-01 00:00:00

abstract：Purpose:Rapid identification of pathogens causing endophthalmitis may improve treatment outcomes through early administration of species-specific medication. The current study reports a new molecular application of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with Staphylococcus-specific molecular...

journal_title：Investigative ophthalmology & visual science

authors： Patel N,Miller D,Relhan N,Flynn HW Jr

更新日期：2017-08-01 00:00:00

abstract：PURPOSE:To investigate the function of connective tissue growth factor (CTGF), a matricellular protein of the CCN (Cyr61/CTGF/Nov) family, in retinal vasculature during development and ischemia. METHODS:CTGF expression was determined using RT-PCR, immunohistochemistry, and transgenic mice carrying CTGF promoter-driven...

journal_title：Investigative ophthalmology & visual science

authors： Pi L,Xia H,Liu J,Shenoy AK,Hauswirth WW,Scott EW

更新日期：2011-11-07 00:00:00

abstract：PURPOSE:DBA/2J mice are a standard preclinical glaucoma model, which spontaneously developed mutations resulting in chronic age-related pigmentary glaucoma. The goals of this study were to identify the degree of visual impairment in DBA/2J mice before and after disease onset by quantifying the optokinetic reflex respon...

journal_title：Investigative ophthalmology & visual science

authors： Burroughs SL,Kaja S,Koulen P

更新日期：2011-06-01 00:00:00

abstract：PURPOSE:To determine associations between retinopathy status and detailed serum lipoprotein subclass profiles in the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications Study (DCCT/EDIC) cohort. METHODS:Persons with type 1 diabetes (440 women, 548 men) from the DCCT/EDIC c...

journal_title：Investigative ophthalmology & visual science

authors： Lyons TJ,Jenkins AJ,Zheng D,Lackland DT,McGee D,Garvey WT,Klein RL

更新日期：2004-03-01 00:00:00

abstract：:Feline sarcoma virus (FeSV) is a naturally occurring virus that causes spontaneous tumors in cats. The immunologic and morphologic characteristics of these tumors have been studied extensively. It was recently observed in experiments undertaken to induce systemic malignancy with this virus, that severe uveitis and cli...

journal_title：Investigative ophthalmology & visual science

authors： Lubin JR,Albert DM,Essex M,de Noronha F,Riis R

更新日期：1983-08-01 00:00:00

abstract：Purpose:To investigate the topographic changes of the dark-adapted multifocal electroretinogram (mfERG) across adulthood in the central retina and compare the topography between macular versus extramacular, nasal versus temporal, and inferior versus superior retinal areas. Methods:Sixty-five subjects (18-88 years) rec...

journal_title：Investigative ophthalmology & visual science

authors： Panorgias A,Tillman M,Sutter EE,Moshiri A,Gerth-Kahlert C,Werner JS

更新日期：2017-02-01 00:00:00

abstract：PURPOSE:Somatostatin (SST) analogues have been used to treat proliferative diabetic retinopathy, pseudotumor cerebri, thyroid orbitopathy, and cystoid macular edema. There is a paucity of published data in regards to cell-specific distribution of SST receptors (SSTR) in normal human eye tissues. Gene expression for all...

journal_title：Investigative ophthalmology & visual science

authors： Klisovic DD,O'Dorisio MS,Katz SE,Sall JW,Balster D,O'Dorisio TM,Craig E,Lubow M

更新日期：2001-09-01 00:00:00

abstract：PURPOSE:Multidrug resistance protein 4 (MRP4) effluxes a wide variety of endogenous compounds, including cyclic adenosine monophosphate (cAMP), and is exclusively expressed in vascular endothelial cells (ECs) of the retina. This study aimed to investigate the role of MRP4 in retinal vascular development. METHODS:The r...

journal_title：Investigative ophthalmology & visual science

authors： Matsumiya W,Kusuhara S,Hayashibe K,Maruyama K,Kusuhara H,Tagami M,Schuetz JD,Negi A

更新日期：2012-12-07 00:00:00

abstract：Purpose:Growing evidence suggests the involvement of the macula even in early stages of glaucoma. However, little is known about the impact of glaucomatous macular damage on central pattern vision. Here we examine the contrast requirement for letter recognition and its relationship with retinal thickness in the macular...

journal_title：Investigative ophthalmology & visual science

authors： Chien L,Liu R,Girkin C,Kwon M

更新日期：2017-12-01 00:00:00

abstract：PURPOSE:In normal eyes and in diseases such as age-related macular degeneration (AMD) and proliferative vitreoretinopathy (PVR), retinal pigment epithelial (RPE) cell survival is critically important. Bcl-x(L) has been shown to be among the most highly expressed survival factors in cultured human RPE cells. In the curr...

journal_title：Investigative ophthalmology & visual science

authors： Zhang N,Peairs JJ,Yang P,Tyrrell J,Roberts J,Kole R,Jaffe GJ

更新日期：2007-08-01 00:00:00

abstract：PURPOSE:Age-related nuclear cataracts involve denaturation and aggregation of intracellular proteins. We have documented age-dependent changes in membrane transport in the mouse lens to see what might initiate changes in the intracellular milieu. METHODS:Microelectrode-based intracellular impedance studies of intact l...

journal_title：Investigative ophthalmology & visual science

authors： Gao J,Wang H,Sun X,Varadaraj K,Li L,White TW,Mathias RT

更新日期：2013-11-01 00:00:00

abstract：PURPOSE:Although the murine alphaA-crystallin promoter is the most commonly used promoter for achieving transgene expression in the developing lens, this promoter directs transgene expression efficiently only in lens fiber cells. The purpose of the present study was to generate promoters capable of directing transgene ...

journal_title：Investigative ophthalmology & visual science

authors： Zhao H,Yang Y,Rizo CM,Overbeek PA,Robinson ML

更新日期：2004-06-01 00:00:00

abstract：PURPOSE:Adipose-derived stem cells (ASCs) have gained importance due to their myriad potential clinical applications. We hypothesize that progenitor cells also exist besides those conventionally isolated from the stromal vascular fraction (SVF). METHOD:Central and medial orbital adipose tissues obtained from patients ...

journal_title：Investigative ophthalmology & visual science

authors： Chen SY,Mahabole M,Horesh E,Wester S,Goldberg JL,Tseng SC

更新日期：2014-07-03 00:00:00

abstract：Purpose:To determine whether combining quantitative optical coherence tomography angiography (OCTA) parameters can achieve high sensitivity and specificity to distinguish eyes with nonproliferative diabetic retinopathy (NPDR) from those with proliferative diabetic retinopathy (PDR) as well as eyes with diabetes and no ...

journal_title：Investigative ophthalmology & visual science

authors： Ashraf M,Nesper PL,Jampol LM,Yu F,Fawzi AA

更新日期：2018-08-01 00:00:00

abstract：PURPOSE:To identify objective criteria to quantify visual function in the rat for developing therapeutic strategies to protect neuronal cells after ischemia. The impact of ocular ischemia on luminance and frequency-modulated contrast vision was compared with the function of outer retinal cells and the number of intact ...

journal_title：Investigative ophthalmology & visual science

authors： Jehle T,Wingert K,Dimitriu C,Meschede W,Lasseck J,Bach M,Lagrèze WA

更新日期：2008-03-01 00:00:00

abstract：PURPOSE:To determine whether the intact isolated ciliary epithelial bilayer retains the alpha-2 and beta adrenergic receptor activation and interaction described for whole ciliary processes and whether this pure epithelial bilayer displays bioelectric parameters sensitive to alterations in cyclic adenosine Monophosphat...

journal_title：Investigative ophthalmology & visual science

authors： Horio B,Sears M,Mead A,Matsui H,Bausher L

更新日期：1996-03-01 00:00:00

abstract：PURPOSE:To quantify the spatial and temporal nature of congenital periodic alternating nystagmus (PAN) and to test the hypothesis that PAN results from a temporal shift in the null zone. METHODS:Twenty-five subjects with oculocutaneous albinism (16 tyrosinase negative and 9 tyrosinase positive) and 7 with ocular albin...

journal_title：Investigative ophthalmology & visual science

authors： Abadi RV,Pascal E

更新日期：1994-11-01 00:00:00

abstract：PURPOSE:We examined changes of the central nervous system in patients with advanced primary open-angle glaucoma (POAG). METHODS:The clinical observational study included 15 patients with bilateral advanced POAG and 15 healthy normal control subjects, matched for age and sex with the study group. Retinal nerve fiber l...

journal_title：Investigative ophthalmology & visual science

authors： Chen WW,Wang N,Cai S,Fang Z,Yu M,Wu Q,Tang L,Guo B,Feng Y,Jonas JB,Chen X,Liu X,Gong Q

更新日期：2013-01-17 00:00:00

abstract：:Axonal transport studies were undertaken to determine the effect of chronic demyelination on axonal function in experimental allergic optic neuritis in the guinea pig, an animal model for multiple sclerosis. Fast and slow components of axonal transport over the prelaminar, laminar, and retrolaminar portions of the opt...

journal_title：Investigative ophthalmology & visual science

authors： Rao NA,Guy J,Sheffield PS

更新日期：1981-10-01 00:00:00

abstract：PURPOSE:In several retinal complications, such as age-dependent macular degeneration (AMD), oxidative stress is increased and cytokine level is elevated. These are shown to alter the activation and expression of matrix metalloproteinase (MMP) both in human primary and immortalized retinal pigment epithelial (RPE) cells...

journal_title：Investigative ophthalmology & visual science

authors： Juuti-Uusitalo K,Nieminen M,Treumer F,Ampuja M,Kallioniemi A,Klettner A,Skottman H

更新日期：2015-10-01 00:00:00

abstract：PURPOSE:To isolate and characterize bovine lens endopeptidase activity that cleaves the Phe-Ser bond in peptide substrates. METHODS:The protease activity in young bovine lens homogenate was measured using the Mca-(Ala(7),Lys(Dnp)(9))-bradykinin substrate. Degradation of bradykinin and other unlabeled peptide substrate...

journal_title：Investigative ophthalmology & visual science

authors： Chaerkady R,Sharma KK

更新日期：2004-04-01 00:00:00

abstract：Purpose:To identify the role of the BBSome protein Bardet-Biedl syndrome 5 (BBS5) in photoreceptor function, protein trafficking, and structure using a congenital mutant mouse model. Methods:Bbs5-/- mice (2 and 9 months old) were used to assess retinal function and morphology. Hematoxylin and eosin staining of retinal...

journal_title：Investigative ophthalmology & visual science

authors： Bales KL,Bentley MR,Croyle MJ,Kesterson RA,Yoder BK,Gross AK

更新日期：2020-08-03 00:00:00