Abstract:
:Cells of Pseudomonas putida NP, Pseudomonas species (NCIB 9816), and a Nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (NAD+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. Similarly, cells of a strain of P. putida biotype A, when grown either on toluene or benzene vapors, were found to contain a dehydrogenase that oxidized dihydrodiols of aromatic hydrocarbons with cis stereochemistry and required NAD+ as an electron acceptor. In all these cases, no enzymatic activity was detected when trans-naphthalene dihydrodiol was used as a substrate. Purified cis-naphthalene dihydrodiol dehydrogenase was injected into rabbits to obtain antibodies. Physiocochemical and immunological properties of cis-dihydrodiol:NAD+ oxidoreductases from four different organisms were examined. Kinetic analysis showed that, in all the cases, enzymes exhibited higher affinity for cis-dihydrodiols than for NAD+ and had pH optima between 8.8 and 9.0. except in the case of the enzyme from Nocarida sp., which showed maximum activity at pH 8.4. Molecular-weight determination of the dehydrogenases from the four different organisms by gel filtration on a Sephadex G-200 column gave values ranging from 92,000 for the enzyme from Nocardia sp. to 160,000 for that from P. putida biotype A. All the dehydrogenases, except the one from Nocardia sp., exhibited immunological cross-reaction with the antibodies prepared against the enzyme purified from P. putida NP.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Patel TR,Gibson DTdoi
10.1128/JB.128.3.842-850.1976subject
Has Abstractpub_date
1976-12-01 00:00:00pages
842-50issue
3eissn
0021-9193issn
1098-5530journal_volume
128pub_type
杂志文章abstract::The D1:1 protein and its potentially occurring degradation products were overexpressed in Escherichia coli. Protein-DNA interaction is shown for the promoter region of psbAI. The D1:1 degradation products may be involved in transcription regulation of psbAI by binding in the promoter region. Additionally, C-terminal f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01428-06
更新日期:2007-03-01 00:00:00
abstract::Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.554-561.1964
更新日期:1964-03-01 00:00:00
abstract::Here we present the genome sequence of Rhizobium grahamii CCGE502. R. grahamii groups with other newly described broad-host-range species, which are not very efficient Phaseolus vulgaris symbionts, with a wide geographic distribution and which constitutes a novel Rhizobium clade. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01785-12
更新日期:2012-12-01 00:00:00
abstract::In acetate-limited chemostat cultures started with single-colony cultures of Thiobacillus versutus, a mutant appeared after approximately 85 volume changes. The inhomogeneity of the culture was detected by the development of two different types of colonies on agar plates. When a pure culture of the mutant was grown in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.791-794.1986
更新日期:1986-11-01 00:00:00
abstract::We examined the role of bacteriochlorophyll synthesis on the regulation of carotenoid synthesis in Rhodopseudomonas capsulata. Strains capable of making bacteriochlorophyll accumulated greater amounts of carotenoids under low oxygen than they did under high oxygen. However, strains unable to produce bacteriochlorophyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1320-1321.1985
更新日期:1985-06-01 00:00:00
abstract::The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.197-201.1984
更新日期:1984-01-01 00:00:00
abstract::The mechanism of methyl-beta-D-thiogalactoside-phosphate (TMG-P) expulsion from Streptococcus pyogenes was studied. The expulsion elicited by glucose was not due to exchange vectorial transphosphorylation between the expelled TMG and the incoming glucose since more beta-galactoside was displaced than glucose taken up,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.354-361.1983
更新日期:1983-10-01 00:00:00
abstract::Enhancement of the production of soluble recombinant penicillin acylase in Escherichia coli via coexpression of a periplasmic protease/chaperone, DegP, was demonstrated. Coexpression of DegP resulted in a shift of in vivo penicillin acylase (PAC) synthesis flux from the nonproductive pathway to the productive one when...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.10.3020-3030.2003
更新日期:2003-05-01 00:00:00
abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1121-1128.1977
更新日期:1977-02-01 00:00:00
abstract::Genetic analysis of a d-alanine requiring mutant (dal) of Bacillus subtilis reveals that the gene that codes for d,l-alanine racemase is linked to purB. The order of genes in this region of the chromosome is purB, pig, tsi, dal. Thus there are at least two clusters of genes that regulate cell wall biosynthesis in B. s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1212-1214.1973
更新日期:1973-09-01 00:00:00
abstract::Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.320-322.1979
更新日期:1979-07-01 00:00:00
abstract::ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00089-12
更新日期:2012-09-01 00:00:00
abstract::The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis by using ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.521-529.1991
更新日期:1991-01-01 00:00:00
abstract::Fitz-James, Philip C. (University of Western Ontario, London, Ont., Canada). Morphology of spore development in Clostridium pectinovorum. J. Bacteriol. 84:104-114. 1962-The process of spore formation in Clostridium pectinovorum was followed by phase-contrast microscopy and by thin-section electron microscopy employing...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.104-114.1962
更新日期:1962-07-01 00:00:00
abstract::The Escherichia coli outer membrane protein Tsx functions as a nucleoside-specific channel and serves as the receptor for colicin K and a number of T-even-type bacteriophages, including phage T6. To identify those segments of the Tsx protein that are important for its phage receptor function, we devised a selection an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.2809-2817.1993
更新日期:1993-05-01 00:00:00
abstract::The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.335-337.1975
更新日期:1975-04-01 00:00:00
abstract::Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-10
更新日期:2010-12-01 00:00:00
abstract::The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1934-1939.1988
更新日期:1988-04-01 00:00:00
abstract::In Escherichia coli anaerobic growth lowers the basal or induced levels of numerous enzymes associated with aerobic metabolism. Mutations in arcA (dye) at min 0 relieve this pleiotropic anaerobic repression and render the cell sensitive to the redox dye toluidine blue. In this study we identified a second pleiotropic ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.868-873.1989
更新日期:1989-02-01 00:00:00
abstract::Protocatechuate 3,4-dioxygenase is a member of a family of bacterial enzymes that cleave the aromatic rings of their substrates between two adjacent hydroxyl groups, a key reaction in microbial metabolism of varied environmental chemicals. In an appropriate genetic background, it is possible to select for Acinetobacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6478-6487.1999
更新日期:1999-10-01 00:00:00
abstract::Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1869-1874.1966
更新日期:1966-05-01 00:00:00
abstract::We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2239-2244.1987
更新日期:1987-05-01 00:00:00
abstract::Members of the carnobacteria have been extensively studied as probiotic cultures in aquacultures and protective cultures in seafood, diary, and meat. We report on the finished genome sequence of Carnobacterium sp. 17-4, which has been isolated from permanently cold seawater. The genetic information reveals a new circu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05113-11
更新日期:2011-07-01 00:00:00
abstract::Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.19.6683-6690.2005
更新日期:2005-10-01 00:00:00
abstract::The incA gene product of ColE2-P9 and ColE3-CA38 plasmids is an antisense RNA that regulates the production of the plasmid-coded Rep protein essential for replication. The Rep protein specifically binds to the origin and synthesizes a unique primer RNA at the origin. The IncB incompatibility is due to competition for ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7233-7243.1994
更新日期:1994-12-01 00:00:00
abstract::Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00211-17
更新日期:2017-09-05 00:00:00
abstract::Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5818-5830.2005
更新日期:2005-08-01 00:00:00
abstract::Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01875-12
更新日期:2013-03-01 00:00:00
abstract::We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1417-1422.1988
更新日期:1988-04-01 00:00:00