Replication of lambda dv plasmid in vitro promoted by purified lambda O and P proteins.

abstract：:Okadaic acid is a non-phorbol 12-myristate 13-acetate (PMA)-type tumor promoter on mouse skin and known to be a potent inhibitor of serine/threonine protein phosphatases. Contrary to expectation from its tumor-promoting activity, okadaic acid was shown to have a potential to revert the phenotypes of cells transformed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakai R,Ikeda I,Kitani H,Fujiki H,Takaku F,Rapp U,Sugimura T,Nagao M

更新日期：1989-12-01 00:00:00

abstract：:Most HLA haplotypes carry two expressed DR beta-chain genes; in the DR4 haplotype, the polymorphic locus has been called DR beta 1 and the apparently nonpolymorphic locus has been called DR beta 2. We have isolated nearly full-length DR beta-chain cDNA clones representing each of these two loci from a cell line homozy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curtsinger JM,Hilden JM,Cairns JS,Bach FH

更新日期：1987-01-01 00:00:00

abstract：:Toll-like receptor 4 (TLR4), the principal signaling receptor for lipopolysaccharide (LPS) in mammals, requires the binding of MD-2 to its extracellular domain for maximal responsiveness. MD-2 contains a leader sequence but lacks a transmembrane domain, and we asked whether it is secreted into the medium as an active ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Visintin A,Mazzoni A,Spitzer JA,Segal DM

更新日期：2001-10-09 00:00:00

abstract：:The use of probes to genes (IG and TCRB) encoding immunoglobulins (IG) and the beta chain of the T-cell antigen receptor (TCRB), respectively, have become a sensitive means to assess clonality and lineage in lymphoid malignancies. It has become apparent that some individual cases show rearrangements of both IG and TCR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davey MP,Bongiovanni KF,Kaulfersch W,Quertermous T,Seidman JG,Hershfield MS,Kurtzberg J,Haynes BF,Davis MM,Waldmann TA

更新日期：1986-11-01 00:00:00

abstract：:Here we report the development of an in vivo system to study the interaction of stem cells with drugs using a tumor model in the adult Drosophila intestine. Strikingly, we find that some Food and Drug Administration-approved chemotherapeutics that can inhibit the growth of Drosophila tumor stem cells can paradoxically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markstein M,Dettorre S,Cho J,Neumüller RA,Craig-Müller S,Perrimon N

更新日期：2014-03-25 00:00:00

abstract：:Acetyl-CoA synthase from Clostridium thermoaceticum (ACS(Ct)) is an alpha(2)beta(2) tetramer containing two novel Ni-X-Fe(4)S(4) active sites (the A and C clusters) and a standard Fe(4)S(4) cluster (the B cluster). The acsA and acsB genes encoding the enzyme were cloned into Escherichia coli strain JM109 and overexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loke HK,Bennett GN,Lindahl PA

更新日期：2000-11-07 00:00:00

abstract：:P2X receptors for ATP have a wide range of physiological roles and comprise a structurally distinct family of ligand-gated trimeric ion channels. The crystal structure of a P2X4 receptor, in combination with mutagenesis studies, has provided a model of the intersubunit ATP-binding sites and identified an extracellular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts JA,Allsopp RC,El Ajouz S,Vial C,Schmid R,Young MT,Evans RJ

更新日期：2012-03-20 00:00:00

abstract：:Controlled maturation of ovarian follicles is necessary for fertility. Follicles are restrained at an immature stage until stimulated by FSH secreted by pituitary gonadotropes. FSH acts on granulosa cells within the immature follicle to inhibit apoptosis, promote proliferation, stimulate production of steroid and prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hunzicker-Dunn ME,Lopez-Biladeau B,Law NC,Fiedler SE,Carr DW,Maizels ET

更新日期：2012-10-30 00:00:00

abstract：:This note presents the derivation of generalized Ambartsumian-Chandrasekhar X and Y functions for stationary transfer in a plane-parallel slab. An algebraic formula relating these functions to the usual reflection function is also presented, together with the appropriate generalization of the Chandrasekhar H-equations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casti J

更新日期：1975-03-01 00:00:00

abstract：:Broken-cell preparations of Salmonella typhimurium rapidly incorporated 75Se from 75SeO3(2-) into tRNA by an ATP-dependent process. Selenium incorporation in the presence of 50 microM 75SeO3(2-) (0.8-1 pmol per A260 unit) was enhanced by the selenocysteine precursor, O-acetyl-L-serine (to 3.7 pmol per A260 unit). This...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Veres Z,Tsai L,Politino M,Stadtman TC

更新日期：1990-08-01 00:00:00

abstract：:In previous studies from this laboratory, the binding regions of alpha-neurotoxins on human and Torpedo acetylcholine (AcCho) receptors (AcChoRs) and the binding regions for the receptor on the toxin were characterized with synthetic peptides of the respective molecules. In the present work, peptides representing the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan KH,Spurlino J,Quiocho FA,Atassi MZ

更新日期：1990-08-01 00:00:00

abstract：:Programmed cell death (PCD) of mammary alveolar cells during involution commences within hours of the end of suckling. Locally, milk accumulates within alveolar lumens; systemically, levels of lactogenic hormones fall. Four experimental models were used to define the role of local factors as compared with systemic hor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li M,Liu X,Robinson G,Bar-Peled U,Wagner KU,Young WS,Hennighausen L,Furth PA

更新日期：1997-04-01 00:00:00

abstract：:ALS is a terminal disease of motor neurons that is characterized by accumulation of proteinaceous deposits in affected cells. Pathological deposition of mutated Cu/Zn superoxide dismutase (SOD1) accounts for ∼20% of the familial ALS (fALS) cases. However, understanding the molecular link between mutation and disease h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivanova MI,Sievers SA,Guenther EL,Johnson LM,Winkler DD,Galaleldeen A,Sawaya MR,Hart PJ,Eisenberg DS

更新日期：2014-01-07 00:00:00

abstract：:Improper function of voltage-gated sodium channels (NaVs), obligatory membrane proteins for bioelectrical signaling, has been linked to a number of human pathologies. Small-molecule agents that target NaVs hold considerable promise for treatment of chronic disease. Absent a comprehensive understanding of channel struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Tran R,Du Bois J

更新日期：2016-05-24 00:00:00

abstract：:Ecological "surprises" are defined as unexpected findings about the natural environment. They are critically important in ecology because they are catalysts for questioning and reformulating views of the natural world, help shape assessments of the veracity of a priori predictions about ecological trends and phenomena...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindenmayer DB,Likens GE,Krebs CJ,Hobbs RJ

更新日期：2010-12-21 00:00:00

abstract：:Chlorpromazine (3 x 10(-4)M) prevents the stimulation of adenyl cyclase activity in thyroid membranes produced by thyrotropin and prostaglandin, ACTH stimulation of adenyl cyclase in adrenal tissue, and glucagon- and epinephrine-stimulation of adenyl cyclase activity in liver. Baseline activity is unaffected. Parathyr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolff J,Jones AB

更新日期：1970-02-01 00:00:00

abstract：:Modern practice for training classification deepnets involves a terminal phase of training (TPT), which begins at the epoch where training error first vanishes. During TPT, the training error stays effectively zero, while training loss is pushed toward zero. Direct measurements of TPT, for three prototypical deepnet a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papyan V,Han XY,Donoho DL

更新日期：2020-10-06 00:00:00

abstract：:Originally identified as an essential component of the herpes simplex virus immediate early (IE) gene enhancer complex, the transcriptional coactivator host cell factor-1 (HCF-1) has been implicated in a broad range of cellular regulatory circuits. The protein mediates activation through multiple interactions with tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narayanan A,Ruyechan WT,Kristie TM

更新日期：2007-06-26 00:00:00

abstract：:Canavan's disease (CD) is a fatal, hereditary disorder of CNS development that has been linked to mutations in the gene for the enzyme aspartoacylase (ASPA) (EC 3.5.1.15). ASPA acts to hydrolyze N-acetylaspartate (NAA) into l-aspartate and acetate, but the connection between ASPA deficiency and the failure of proper C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Madhavarao CN,Arun P,Moffett JR,Szucs S,Surendran S,Matalon R,Garbern J,Hristova D,Johnson A,Jiang W,Namboodiri MA

更新日期：2005-04-05 00:00:00

abstract：:To initiate transcription from specific promoters, the bacterial RNA polymerase (RNAP) core enzyme must associate with the initiation factor sigma, which contains determinants that allow sequence-specific interactions with promoter DNA. Most bacteria contain several sigma factors, each of which directs recognition of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yuan AH,Nickels BE,Hochschild A

更新日期：2009-04-21 00:00:00

abstract：:Networks are widely used in the biological, physical, and social sciences as a concise mathematical representation of the topology of systems of interacting components. Understanding the structure of these networks is one of the outstanding challenges in the study of complex systems. Here we describe a general techniq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newman ME,Leicht EA

更新日期：2007-06-05 00:00:00

abstract：:Mass cytometry enables an unprecedented number of parameters to be measured in individual cells at a high throughput, but the large dimensionality of the resulting data severely limits approaches relying on manual "gating." Clustering cells based on phenotypic similarity comes at a loss of single-cell resolution and o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shekhar K,Brodin P,Davis MM,Chakraborty AK

更新日期：2014-01-07 00:00:00

abstract：:Oil-in-water emulsions are potent human adjuvants used for effective pandemic influenza vaccines; however, their mechanism of action is still unknown. By combining microarray and immunofluorescence analysis, we monitored the effects of the adjuvants MF59 oil-in-water emulsion, CpG, and alum in the mouse muscle. MF59 i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mosca F,Tritto E,Muzzi A,Monaci E,Bagnoli F,Iavarone C,O'Hagan D,Rappuoli R,De Gregorio E

更新日期：2008-07-29 00:00:00

abstract：:Atmospheric models generally assume that aerosol particles are in equilibrium with the surrounding gas phase. However, recent observations that secondary organic aerosols can exist in a glassy state have highlighted the need to more fully understand the kinetic limitations that may control water partitioning in ambien...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bones DL,Reid JP,Lienhard DM,Krieger UK

更新日期：2012-07-17 00:00:00

abstract：:Primitive cells capable of generating small resistance arterioles and capillary structures in the injured myocardium have been identified repeatedly. However, these cells do not form large conductive coronary arteries that would have important implications in the management of the ischemic heart. In the current study,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bearzi C,Leri A,Lo Monaco F,Rota M,Gonzalez A,Hosoda T,Pepe M,Qanud K,Ojaimi C,Bardelli S,D'Amario D,D'Alessandro DA,Michler RE,Dimmeler S,Zeiher AM,Urbanek K,Hintze TH,Kajstura J,Anversa P

更新日期：2009-09-15 00:00:00

abstract：:Viperin is an IFN-inducible radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication. We determined crystal structures of an anaerobically prepared fragment of mouse viperin (residues 45-362) complexed with S-adenosylhomocysteine (SAH) or 5'-deoxyadenosine (5'-dAdo) and l-methionine (l-Met). Viperin c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fenwick MK,Li Y,Cresswell P,Modis Y,Ealick SE

更新日期：2017-06-27 00:00:00

abstract：:Molecules of transfer RNA (tRNA) typically contain four stems composed of Watson-Crick (W-C) base pairs and infrequent mispairs such as G-U and A-C. The latter mispairs are fundamental units of RNA secondary structure found in nearly every class of RNA and are nearly isomorphic to W-C pairs. Therefore, they often subs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClain WH

更新日期：2006-03-21 00:00:00

abstract：:A rice Dwarf 1 gene was identified by using a map-based cloning strategy. Its recessive mutant allele confers a dwarf phenotype. Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3,185 d1 segregants. Southern hybridization analysis with this cDNA as a probe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ashikari M,Wu J,Yano M,Sasaki T,Yoshimura A

更新日期：1999-08-31 00:00:00

abstract：:Like other guanine nucleotide-exchange proteins (GEPs) that activate ADP-ribosylation factor (ARF) GTPases, brefeldin A-inhibited GEP2, BIG2, contains an approximately 200-aa Sec7 domain that is responsible for this catalytic activity and its inhibition by brefeldin A. The Sec7 domain is located near the center of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Adamik R,Pacheco-Rodriguez G,Moss J,Vaughan M

更新日期：2003-02-18 00:00:00

abstract：:Transgenic mice have provided invaluable information about gene function and regulation. However, because of marked differences between rodents and primates, some areas of human biology such as early embryonic development, aging, and maternal-fetal interactions would be best studied in a nonhuman primate model. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolfgang MJ,Eisele SG,Browne MA,Schotzko ML,Garthwaite MA,Durning M,Ramezani A,Hawley RG,Thomson JA,Golos TG

更新日期：2001-09-11 00:00:00