Modified spray for the detection of phospholipids on thin-layer chromatograms.

abstract：:There is unequivocal evidence of an inverse association between plasma high-density lipoprotein (HDL) cholesterol concentrations and the risk of cardiovascular disease, a finding that has led to the hypothesis that HDL protects from atherosclerosis. This review details the experimental evidence for this "HDL hypothesi...

journal_title：Journal of lipid research

authors： Vergeer M,Holleboom AG,Kastelein JJ,Kuivenhoven JA

更新日期：2010-08-01 00:00:00

abstract：:Membranes of mammalian cells contain lysophosphatidic acid acyltransferase (LPAAT) activities that catalyze the acylation of sn-1-acyl lysophosphatidic acid (lysoPA) to form phosphatidic acid. As the biological roles and biochemical properties of the six known LPAAT isoforms have yet to be fully elucidated, we have ch...

journal_title：Journal of lipid research

authors： Hollenback D,Bonham L,Law L,Rossnagle E,Romero L,Carew H,Tompkins CK,Leung DW,Singer JW,White T

更新日期：2006-03-01 00:00:00

abstract：:Acyl-coenzyme A:diacylglycerol transferase (DGAT), fatty acid synthetase (FAS), and LPL are three enzymes important in adipose tissue triglyceride accumulation. To study the relationship of DGAT1, FAS, and LPL with insulin, we examined adipose mRNA expression of these genes in subjects with a wide range of insulin sen...

journal_title：Journal of lipid research

authors： Ranganathan G,Unal R,Pokrovskaya I,Yao-Borengasser A,Phanavanh B,Lecka-Czernik B,Rasouli N,Kern PA

更新日期：2006-11-01 00:00:00

abstract：:A two-step polymerase chain reaction (PCR) method for the rapid detection of the apolipoprotein B(Arg3500----Gln) mutation in a mixture of pooled blood samples is described. In the first step PCR, a short gene fragment surrounding codon 3500 is amplified. Subsequently the reaction product is subjected to a second ampl...

journal_title：Journal of lipid research

authors： Ruzicka V,März W,Russ A,Gross W

更新日期：1992-10-01 00:00:00

abstract：:Dietary n-3 PUFAs have been shown to attenuate T-cell-mediated inflammation. To investigate whether dietary n-3 PUFAs promote activation-induced cell death (AICD) in CD4+ T-cells induced in vitro to a polarized T-helper1 (Th1) phenotype, C57BL/6 mice were fed diets containing either 5% corn oil (CO; n-6 PUFA control) ...

journal_title：Journal of lipid research

authors： Switzer KC,Fan YY,Wang N,McMurray DN,Chapkin RS

更新日期：2004-08-01 00:00:00

abstract：:Diseases such as obesity, diabetes, and atherosclerosis result from multiple genetic and environmental factors, and importantly, interactions between genetic and environmental factors. Identifying susceptibility genes for these diseases using genetic and genomic technologies is accelerating, and the expectation over t...

journal_title：Journal of lipid research

authors： Schadt EE,Lum PY

更新日期：2006-12-01 00:00:00

abstract：:To study the role of phospholipids in the lipophorin structure, they were removed by phospholipase A2 treatment. Fluorescence lifetimes and accessibility to acrylamide quenching of different diphenylhexatrienyl derivatives, which were used as analogues of the different lipid components, indicate a surface localization...

journal_title：Journal of lipid research

authors： Rimoldi OJ,Garda HA,Brenner RR

更新日期：1996-10-01 00:00:00

abstract：:The action of cycloheximide on some parameters of glucose and lipid metabolism was studied in vitro in epidiymal fat pads from fasted rats. Incubation of fat pads with cycloheximide (1 mug/ml) for 2 hours resulted in a two-fold increase in glucose uptake, glucose oxidation, incorporation of glucose into lipids, and re...

journal_title：Journal of lipid research

authors： García-Sáinz JA,Piña E,Chagoya de Sánchez V

更新日期：1977-01-01 00:00:00

abstract：:Cholesteryl ester storage disease (CESD) and Wolman disease (WD) are both autosomal recessive disorders associated with reduced activity and genetic defects of lysosomal acid lipase (LAL). The strikingly more severe course of WD is caused by genetic defects of LAL that leave no residual enzymatic activity. Mutations a...

journal_title：Journal of lipid research

authors： Ries S,Aslanidis C,Fehringer P,Carel JC,Gendrel D,Schmitz G

更新日期：1996-08-01 00:00:00

abstract：:Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employi...

journal_title：Journal of lipid research

authors： Reading BJ,Hiramatsu N,Schilling J,Molloy KT,Glassbrook N,Mizuta H,Luo W,Baltzegar DA,Williams VN,Todo T,Hara A,Sullivan CV

更新日期：2014-11-01 00:00:00

abstract：:Recent evidence suggests the possibility that macrophages can influence lipoprotein metabolism. Therefore we investigated the ability of cultured macrophages to alter low density lipoprotein (LDL) uptake in a human liver cell line (HepG2). Conditioned media from phlogogenic-induced mouse peritoneal macrophages or from...

journal_title：Journal of lipid research

authors： Grove RI,Mazzucco C,Allegretto N,Kiener PA,Spitalny G,Radka SF,Shoyab M,Antonaccio M,Warr GA

更新日期：1991-12-01 00:00:00

abstract：:Distinct isoforms of long-chain acyl-CoA synthetases (ACSLs) may partition fatty acids toward specific metabolic cellular pathways. For each of the five members of the rat ACSL family, we analyzed tissue mRNA distributions, and we correlated the mRNA, protein, and activity of ACSL1 and ACSL4 after fasting and refeedin...

journal_title：Journal of lipid research

authors： Mashek DG,Li LO,Coleman RA

更新日期：2006-09-01 00:00:00

abstract：:Common forms of metabolic and cardiovascular diseases involve the interplay of numerous genes as well as important environmental factors. Traditional biochemical and genetic approaches generally attempt to dissect these diseases one gene at a time, for example, by analysis of Mendelian forms or genetically engineered ...

journal_title：Journal of lipid research

authors： Wu S,Lusis AJ,Drake TA

更新日期：2009-04-01 00:00:00

abstract：:Over the past several years, considerable progress has been made in the development of gene therapy as a therapeutic strategy for a variety of inherited metabolic diseases, including neuropathic lysosomal storage disorders (LSDs). The premise of gene therapy for this group of diseases is borne of findings that genetic...

journal_title：Journal of lipid research

authors： Cheng SH

更新日期：2014-09-01 00:00:00

abstract：:We previously have shown that LDL-R354, a truncated low density lipoprotein (LDL) receptor, is a calcium binding protein. LDL-R354 is composed of the ligand binding domain and repeat A of the EGF precursor homology domain of the full-length human LDL receptor. We also found that Ca2+ was required for the interaction b...

journal_title：Journal of lipid research

authors： Dirlam-Schatz KA,Attie AD

更新日期：1998-02-01 00:00:00

abstract：:Apart from exhibiting the presence of lipoprotein [a] in its plasma, another interest of the European hedgehog in lipoprotein research lies in the quantitative prominence of a complex spectrum of high density lipoproteins (HDL) and very high density lipoproteins (VHDL) as cholesterol transporters in plasma (Laplaud, P...

journal_title：Journal of lipid research

authors： Sparrow DA,Laplaud PM,Saboureau M,Zhou G,Dolphin PJ,Gotto AM Jr,Sparrow JT

更新日期：1995-03-01 00:00:00

abstract：:Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator that promotes cell survival, proliferation, migration, angiogenesis, lymphangiogenesis, and immune response; all are critical processes of cancer progression. Although some important roles of intracellular S1P have recently been uncovered, the majority of its...

journal_title：Journal of lipid research

authors： Takabe K,Spiegel S

更新日期：2014-09-01 00:00:00

abstract：:We have shown that expression of apolipoprotein (apo) C-III promotes VLDL secretion from transfected McA-RH7777 cells under lipid-rich conditions. To determine structural elements within apoC-III that confer to this function, we contrasted wild-type apoC-III with a mutant Ala23Thr originally identified in hypotriglyce...

journal_title：Journal of lipid research

authors： Sundaram M,Zhong S,Bou Khalil M,Zhou H,Jiang ZG,Zhao Y,Iqbal J,Hussain MM,Figeys D,Wang Y,Yao Z

更新日期：2010-06-01 00:00:00

abstract：:Mice with a targeted mutation of 3beta-hydroxysterol Delta(7)-reductase (Dhcr7) that cannot convert 7-dehydrocholesterol to cholesterol were used to identify the origin of fetal sterols. Because their heterozygous mothers synthesize cholesterol normally, virtually all sterols found in a Dhcr7 knockout fetus having a D...

journal_title：Journal of lipid research

authors： Tint GS,Yu H,Shang Q,Xu G,Patel SB

更新日期：2006-07-01 00:00:00

abstract：:Cholesterol monohydrate crystal formation was studied in supersaturated model bile solutions, containing unlabeled cholesterol, sodium cholate and soybean phosphatidylcholine, and tracer amounts of [3H]cholesterol. Solutions were either seeded with cholesterol crystals to initiate growth, or not seeded to allow self-n...

journal_title：Journal of lipid research

authors： Whiting MJ,Watts JM

更新日期：1983-07-01 00:00:00

abstract：:Semi-purified diets supplemented with either a high linoleate (n-6) (safflower) oil or a high alpha-linolenate (n-3) (perilla) oil were fed to mouse mothers and their offspring through 6 weeks of age. The proportions of n-3 and n-6 highly unsaturated fatty acids in brain phospholipids reflected the n-3/n-6 balance of ...

journal_title：Journal of lipid research

authors： Nakashima Y,Yuasa S,Hukamizu Y,Okuyama H,Ohhara T,Kameyama T,Nabeshima T

更新日期：1993-02-01 00:00:00

abstract：:Mitochondrial sterol 27-hydroxylase (EC 1.14.13.15) is an important enzyme, not only in the formation of bile acids from cholesterol intermediates in the liver but also in the removal of cholesterol by side chain hydroxylation in extrahepatic tissues. The enzyme has been assayed by complicated methods using radiolabel...

journal_title：Journal of lipid research

authors： Ota Y,Eto TA,Tanaka S,Sueta H,Shiotsuki H,Maeda Y,Une M,Chijiiwa K

更新日期：2003-12-01 00:00:00

abstract：:Previous in vitro studies have revealed that oxidized low density lipoprotein (OxLDL) has negative effects on the proliferation and activity of endothelial progenitor cells (EPCs). Here, we evaluated the effect of OxLDL on the therapeutic potential of EPCs in ischemia-induced neovascularization. EPCs derived from mobi...

journal_title：Journal of lipid research

authors： Zhou B,Ma FX,Liu PX,Fang ZH,Wang SL,Han ZB,Poon MC,Han ZC

更新日期：2007-03-01 00:00:00

abstract：:N-acylethanolamines (NAEs) are a group of lipid mediators synthesized in response to a number of physiological and pathological stimuli. Because of the low tissue concentrations of NAEs, analyses often include liquid extraction followed by solid-phase extraction and subsequent quantitation by LC/MS or GC/MS. Reported ...

journal_title：Journal of lipid research

authors： Skonberg C,Artmann A,Cornett C,Hansen SH,Hansen HS

更新日期：2010-10-01 00:00:00

abstract：:Ketone bodies (KBs), acetoacetate and β-hydroxybutyrate (βHB), were considered harmful metabolic by-products when discovered in the mid-19th century in the urine of patients with diabetic ketoacidosis. It took physicians many years to realize that KBs are normal metabolites synthesized by the liver and exported into t...

journal_title：Journal of lipid research

authors： Hashim SA,VanItallie TB

更新日期：2014-09-01 00:00:00

abstract：:HDL cholesterol levels are decreased in Crohn's disease, a tumor necrosis factor-alpha (TNF-alpha)-driven chronic inflammatory condition involving the gastrointestinal tract. ATP-binding cassette transporter A1 (ABCA1), one of several liver X receptor (LXR) target genes, is a cell surface transporter that mediates the...

journal_title：Journal of lipid research

authors： Field FJ,Watt K,Mathur SN

更新日期：2010-06-01 00:00:00

abstract：:This report describes the gas-liquid chromatography-mass spectrometry (GLC-MS) of the trimethylsilyl ethers of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol with mono- or dihydroxy substitution in the side chain. Compounds with 24- and 25-unsaturation in the side chain were also studied. The gas-liquid chromatogr...

journal_title：Journal of lipid research

authors： Tint GS,Dayal B,Batta AK,Shefer S,Cheng FW,Salen G,Mosbach EH

更新日期：1978-11-01 00:00:00

abstract：:Investigations of the topography of glycerolipid synthetic enzymes within the transverse plane of microsomal vesicles indicated an exclusive cytoplasmic surface location of active sites. Evidence was derived from studies employing proteases and other impermeant inhibitors, from investigations of latency and substrate ...

journal_title：Journal of lipid research

authors： Bell RM,Ballas LM,Coleman RA

更新日期：1981-03-01 00:00:00

abstract：:The rate of removal of phosphatidylethanolamine and phosphatidylcholine from the plasma of rats treated with antiserum to hepatic lipase was measured. The hepatic lipase antiserum was injected intravenously into animals prior to injection of 32P-labeled chylomicrons or 32P-labeled high density lipoproteins. In experim...

journal_title：Journal of lipid research

authors： Landin B,Nilsson A,Twu JS,Schotz MC

更新日期：1984-06-01 00:00:00

abstract：:Imidazole catalyzed acylations of lysolipids by acyl-CoAs in water at room temperature and at a pH close to neutrality. In the presence of oleoyl-CoA and either lysophosphatidylcholine, 1-palmitoyl-sn-glycero-3-phosphocholine (LPC); lysophosphatidylglycerol, monoacyl-sn-glycero-3-phosphoglycerol; lysophosphatidyl acid...

journal_title：Journal of lipid research

authors： Testet E,Akermoun M,Shimoji M,Cassagne C,Bessoule JJ

更新日期：2002-07-01 00:00:00