Abstract：
:Suitable procedures for separation of various stages of Toxoplasma gondii by density gradient centrifugation have been delineated using peritoneal exudate of infected mouse as a model. Separation from host cells was effected by gradient centrifugation at 450 g for 30 minutes. Using Ficoll, dextran and sucrose, average parasite recoveries by pooling up to and including the peak fraction of 73.65%, 66.18% and 65.68% respectively were obtained. Toxoplasma trophozoites peaked at density of 1.040 g/ml with Ficoll, 1.060 g/ml with dextran and 1.110 g/ml with sucrose. In view of successful separation of exo-enteric stages of Toxoplasma by density gradient centrifugation, possible application of this method to isolation of various endo-enteric stages is discussed.
journal_name
Folia Parasitol (Praha)journal_title
Folia parasitologicaauthors
Masihi KN,Jíra Jsubject
Has Abstractpub_date
1979-01-01 00:00:00pages
9-13issue
1eissn
0015-5683issn
1803-6465journal_volume
26pub_type
杂志文章abstract：:The paper deals with the problem of interrelationship between macroclimate of the region, mesoclimate of the biotope studied and microclimate of the tick niches proper, necessary for the research of tick ecology. New methods for the solution of microclimatological studies are proposed and the relationship between macr...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Faecal samples from the rock hyrax (Procavia capensis jayakari Thomas) were collected from the Ibex Reserve in central Saudi Arabia. Eimerian oocysts, which are believed to represent a new species described here as Eimeria tamimi sp. n., were detected in 40 out of 93 samples. Oocysts were fully sporulated in 24-48 hou...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2020.001
更新日期：2020-01-10 00:00:00
abstract：:Neopolystoma liewi sp. n. is described from the conjunctival cavity of the Malayan box turtle Cuora amboinensis (Daudin, 1802), in Peninsular Malaysia. This is the first record of Neopolystoma in Malaysia and the fourth polystomatid species described from C. amboinensis. Of the 27 Malayan box turtles examined, 8 were ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2000.003
更新日期：2000-01-01 00:00:00
abstract：:Hemolivia mauritanica (Sergent et Sergent, 1904) infections were found in 14% (n= 14) of Testudo graeca Linnaeus, 1758 tortoises in Bulgaria and in 92% (n = 26) of those in Turkey. Hemolivia mauritanica-like gametocytes were found in 81% (n = 47) of wild Testudo marginata Schoepff, 1792 tortoises in Greece. Parasitaem...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Investigations on the epizoic fauna of Gadus morhua (L.), Platichthys flesus (L.) and Oncorhynchus mykiss (Walbaum) from the Kiel Fjord and Kiel Bight were carried out from September 1996 to March 1997. Smears from 120 G. morhua and 92 P. flesus caught using fish traps and trammel nets, and of 35 O. mykiss obtained fr...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2000.018
更新日期：2000-01-01 00:00:00
abstract：:The Capsalidae are monogeneans parasitizing 'skin', fins and gills of marine fishes. Some capsalids are pathogenic to cultivated fish and a few have caused epizootic events. It is a cosmopolitan family with broad host associations (elasmobranchs and teleosts, including sturgeons). Approximately 200 capsalid species ar...
journal_title：Folia parasitologica
pub_type： 杂志文章,评审
doi：10.14411/fp.2004.016
更新日期：2004-06-01 00:00:00
abstract：:In the course of the study "Toxoplasmosis and Prematurity" 330 blood samples from twins were examined. Our findings in a series of 21 premature twins (maternal sera were also examined) are reported in this paper. Toxoplasma antibodies were detected by the Sabin-Feldman test and specific IgM antibodies by the Remington...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Three foxes about 18 weeks old were each subcutaneously inoculated with approximately 200 LD50 dose of extraneurally passaged strain of the Tahyna virus "236". Viremia was demonstrated in all animals during the 48-96 hours interval after infection. The maximum value was 1.31 dex LD50/0.02 ml. Three weeks after infecti...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:In order to investigate the ability of infective larvae of the nematode Baylisascaris transfuga (Rudolphi, 1819) Sprent, 1968 to hatch from the egg-shells and then to migrate in tissues, parenteral infections of mice with embryonated eggs were performed. Two groups of outbred albino mice were infected with approximate...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Changes in behaviour and cognition have been associated with latent infection from the apicomplexan protozoan Toxoplasma gondii (Nicolle et Manceaux, 1908) in both animal and human studies. Further, neuropsychiatric disorders such as schizophrenia have also been associated with latent toxoplasmosis. Previously, we fou...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2016.032
更新日期：2016-09-26 00:00:00
abstract：:A new genus of the family Capillariidae, Tridentocapillaria gen. n., has been established. A type species is Tridentocapillaria tridens (Dujardin, 1845) comb. n. Other species of this genus are T. eurycerca (Oschmarin et Parukhin, 1963) comb. n., T. parusi (Wakelin, Schmidt et Kuntz, 1970) comb. n., and T. javanensis ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Previously published multidisciplinary studies in the Miramichi and Bouctouche rivers (New Brunswick, Canada) noted significant changes in fish health parameters, including elevated tissue levels of organic contaminants and a wide range of physiological disturbances, in mummichog Fundulus heteroclitus (L.) from a site...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2011.023
更新日期：2011-09-01 00:00:00
abstract：:Mastacembelocleidus gen. n. (Monogenoidea: Dactylogyridae) is proposed to include two species collected and redescribed from spiny eels (Mastacembelidae) in India and Iraq: Mastacembelocleidus bam (Tripathi, 1959) comb. n. (syn. Ancyrocephalus bam Tripathi, 1959) from the gills of Macrognathus pancalus (new host recor...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2004.036
更新日期：2004-12-01 00:00:00
abstract：:Ixodid ticks remain attached to their hosts for several days to weeks. During this extended feeding process new proteins involved in the modulation of host immune responses are expressed in tick salivary glands. In our study a stimulatory or inhibitory effect of salivary gland extracts (SGE) of unfed and partially fed...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:Pseudorhabdosynochus yucatanensis sp. n. (Diplectanidae) is described from the gills of the red grouper, Epinephelus morio (Valenciennes, 1824) (Serranidae), from the coast of the Yucatan Peninsula, Mexico. This new species is characterized by having both the ventral and the dorsal squamodiscs composed of 10-12 rows o...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:A new nematode species, Paraphilometroides nemipteri sp. n. is described from the female specimens collected from the dorsal fin and operculum of the marine perciforme fish, Nemipterus peronii off Kuala Terengganu coastal waters in Malaysia. It considerably differs from all other species in Philometridae in the struct...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Bloodforms of Trypanosoma brucei brucei STIB 247 taken from rats and containing more than 80 per cent short stumpy forms, differentiated in vitro to procyclic forms in medium SDM 79 (Brun and Schönenberger 1979), enriched with 3 mmol.dm-3 cis-aconitate. Cell division was abolished by the addition of hydroxyurea (200 m...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Soricinia tripartita Zarnowski, 1955 is redescribed on the basis of specimens from the type host Sorex araneus Linnaeus from Lithuania, Latvia and Russia (Republic of Karelia and Republic of Komi - a new geographical record) as well as from Sorex satunini Ognev and Sorex volnuchini Ognev from Russia (Nalchik Area in t...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：:A part of the life cycle of Proteocephalus neglectus La Rue, 1911, a parasite of trout, starting from release of eggs from mature parasites into water, to the early phase of development in the definitive host, was studied under experimental conditions. Special regard has been paid to development in the intermediate ho...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Isolation and characterisation of Plasmodium falciparum (Welch, 1897) soluble antigens from infected patient plasma, Western blotting, thermal stability and ELISA assays using hyperimmune IgG-antimalaria antibodies was the main objective of this work. A circulating antigen of approximately Mr 33-35 kDa with good speci...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2000.046
更新日期：2000-01-01 00:00:00
abstract：:The cestode fauna of the darkspotted numbfish, Narcine maculata (Shaw) (Torpediniformes: Narcinidae), from Malaysian Bomrneo was examined for the first time. This work resulted in the discovery of a new genus and two new species of Anteroporidae (Lecanicephalidea). Sesquipedalapex comicus gen. n., sp. n. was erected o...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2011.010
更新日期：2011-06-01 00:00:00
abstract：:Cucolepis gen. n. is erected as monotypic for Cucolepis cincta sp. n., a new species of cyclophyllidean cestode of the family Paruterinidae. The new species is described from the squirrel cuckoo, Piaya cayana Lesson (Aves: Cuculiformes), taken from two localities in Paraguay in 1984 and 1985. This new genus is most si...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2012.040
更新日期：2012-12-01 00:00:00
abstract：:Parasitological examination of faeces of 26 snakes kept in Bio-Ken Snake Farm, Watamu, Kenya revealed new species of Eimeria Schneider, 1875 in Telescopus semiannulatus Smith, 1849. Oocysts of Eimeria arabukosokokensis sp. n. are cylindrical 26.8 (25-29) x 15.1 (14-16) microm with smooth, bilayered oocyst wall and a s...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2003.004
更新日期：2003-03-01 00:00:00
abstract：:A relatively high rate of mortality among engorged females of Ornithodoros moubata (Murray, 1877) was observed in our laboratory colony. The general aim of the study was to identify the causative agent responsible for this mortality. The diagnostic tests were performed by Yeast Identification Service (CBS-Delft, Nethe...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:This work describes the ecological characteristics of the intestinal helminth communities of 50 wolves (Canis lupus L.) from Spain. The species found were classified into three groups according to prevalence, intensity and intestinal distribution. Taenia hydatigena Pallas, 1766 and Uncinaria stenocephala (Railliet, 18...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2003.041
更新日期：2003-09-01 00:00:00
abstract：:The trichostrongylid nematode Travassostrongylus scheibelorum sp. n. from the Linnaeus' mouse opossum, Marmosa murina (Linnaeus) (type host), and the woolly mouse opossum, Marmosa demerarae (Thomas), from French Guiana is described. The nematodes have a synlophe with ridges frontally oriented from right to left, six d...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2014.032
更新日期：2014-06-01 00:00:00
abstract：:Parasitological examinations of 102 specimens of the lesser great cormorant Phalacrocorax carbo sinensis (Blumenbach) from two nesting regions in the Czech Republic (South Bohemia and South Moravia) were carried out at the Institute of Parasitology, Czech Academy of Sciences (previously the Czechoslovak Academy of Sci...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2016.022
更新日期：2016-06-13 00:00:00
abstract：:During the 3rd Czechoslovak mountaineering expedition to the Himalaya in the spring 1973 ticks of the family Ixodidae were collected from small mammals and additionally from pastured sheep, and humans. The tick material represented the following species: Ixodes (P.) ovatus Neumann, 1899, Ixodes (I.) acutitarsus (Karsc...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The following four species (only females available) of the Philometridae (Nematoda: Dracunculoidea) were recorded from freshwater fishes of Lake Turkana, northwestern Kenya in 2007-2008: Philometra bagri (Khalil, 1965) from the subcutaneous tissue around the mouth, on gill covers and the fin base of the bayad Bagrus b...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2009.008
更新日期：2009-03-01 00:00:00
abstract：:Two squirrels aged 16 weeks and three muskrats aged 24 weeks were subcutaneously infected with a dose of 400 SMicLD50 of the extraneurally passaged "236" strain of Tahyna virus. Viremia was detected in one squirrel (48 and 96 hours post infection) and in two muskrats (24 and 48 hours p.i.). Seroconversion was demonstr...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
