Abstract:
:Bloodforms of Trypanosoma brucei brucei STIB 247 taken from rats and containing more than 80 per cent short stumpy forms, differentiated in vitro to procyclic forms in medium SDM 79 (Brun and Schönenberger 1979), enriched with 3 mmol.dm-3 cis-aconitate. Cell division was abolished by the addition of hydroxyurea (200 micrograms.ml-1) or arabinosyl adenine (20 micrograms.ml-1 to the cultivation medium, or by the omission of serum from the medium. The ultrastructure of exponentially growing controls was rearranged within 24 h. The endogenous respiration and the respiration stimulated by proline, succinate, and 2-oxoglutarate were detectable within 12 h; after 48 h the respiration rates were comparable to those found in the established procyclic forms. After 12 h the respiration was inhibited by 200 mumol.dm-3 KCN, and by 20 mumol.dm-3 antimycin to the extent found in procyclic forms. Hydroxyurea did not significantly affect respiration. Activities of procyclic-stage enzyme markers malate dehydrogenase, threonine dehydrogenase, succinate: cytochrome c reductase, and NADH: cytochrome c reductase rose within 48 h of differentiation to values which were close to those found in established procyclic forms. The activity of glutamate dehydrogenase (NAD-specific), however, was only 1/3 of that in the procyclics, and no citrate synthase was detected in differentiating culture. Glycosomal malate dehydrogenase was detected after 6 h. In the presence of hydroxyurea or arabinosyl adenine, or in the absence of serum, respiration rates, marker enzyme activities, and glycosomal malate dehydrogenase developed to the extent comparable to the untreated controls. The results suggest that it is possible to separate the process of differentiation from cell proliferation. Cell division is not a necessary prerequisite of differentiation.
journal_name
Folia Parasitol (Praha)journal_title
Folia parasitologicaauthors
Markos A,Blahůsková A,Kalous M,Bysková E,Byska K,Nohýnková Esubject
Has Abstractpub_date
1989-01-01 00:00:00pages
225-38issue
3eissn
0015-5683issn
1803-6465journal_volume
36pub_type
杂志文章abstract::The tissue reaction to Cysticercus bovis in the lung of cattle with an experimental infection was an inflammatory rim originating in the immediate vicinity of the cysts. The cysts recovered at days 83 and 102 p.i. contained living cysticerci. The rim was composed either of a layer of high histiocytes organized in pali...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::The adult morphology is described and illustrated of Neoplagioporus kajika sp. n. (Digenea: Opecoelidae) found in the Japanese fluvial sculpin Cottus pollux Günther (Osteichthyes: Scorpaeniformes: Cottidae) collected in the Naka River at Terase Bridge, Narutake, Nakagawa Town, Fukuoka Prefecture, Kyushu, Japan. This n...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2006.026
更新日期:2006-09-01 00:00:00
abstract::Ultrastructural characters of spermiogenesis and mature spermatozoon of Triaenorhina rectangula (Fuhrmann, 1908) are examined by transmission electron microscopy. Spermiogenesis follows the Bâ and Marchand's Type III spermiogenesis of cestodes. The process begins with the formation of a differentiation zone containing...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2009.032
更新日期:2009-12-01 00:00:00
abstract::A short survey is given of the occurrence of babesiasis affecting small terrestrial mammals in some parts of Europe. Results obtained in studies of 7,038 small terrestrial mammals (42 species) from Czechoslovakia, Austria, Hungary, Yugoslavia and Bulgaria, show the distribution of Babesia microti in these countries. T...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Large and small rDNA sequences of 41 species of the family Opecoelidae are utilised to produce phylogenetic inference trees, using brachycladioids and lepocreadioids as outgroups. Sequences were newly generated for 13 species. The resulting Bayesian trees show a monophyletic Opecoelidae. The earliest divergent group i...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2016.013
更新日期:2016-04-20 00:00:00
abstract::Life cycle of Eimeria coecicola was studied in experimentally infected rabbits by light microscopy and by transmission and scanning electron microscopy. First and second generation meronts developed in the vermiform appendix; third and fourth generation meronts were located in the epithelium of the ileum. Gametogony d...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The chemoprophylactic and chemotherapeutic effects of levamisole and mebendazole on Angiostrongylus malaysiensis infection in rats were studied. Both drugs were ineffective in preventing infection while the post-infection treatment showed 100% efficacy. Furthermore, levamisole and mebendazole when given in combination...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Primary amoebic meningoencephalitis (PAM) was induced in mice by intranasal inoculation of Naegleria fowleri (Singh et Das, 1970) to study the role of the blood vessels and lungs in the early and later stages in this disease. Upon culturing blood and lung tissue obtained at 24-, 36-, 48-, 72-, 96-, and 120-hour time p...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2002.035
更新日期:2002-01-01 00:00:00
abstract::An ICR outbred suckling mouse model of cryptosporidiosis was used to explain some of the variability associated with experimental Cryptosporidium parvum infections in neonate mice. Fourty-four groups of 12 mice each, ranging in age from 4-12 days, each received 1.0 x 10(4) CsCl purified oocysts per os in 5 microns PBS...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Results are presented of a serological examination of 1,547 domestic animals (cattle, pig, sheep, horse, goat, dog, cat) from 9 Tyrolian districts (Austria), performed in order to disclose the incidence of leptospirosis. Completely significant titres were domonstrated by means of the MAL test in the serotypes icteroha...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::In order to investigate the ability of infective larvae of the nematode Baylisascaris transfuga (Rudolphi, 1819) Sprent, 1968 to hatch from the egg-shells and then to migrate in tissues, parenteral infections of mice with embryonated eggs were performed. Two groups of outbred albino mice were infected with approximate...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Monoclonal antibody B4 (mAb B4) was previously developed to the myxozoan parasite Tetracapsuloides bryosalmonae Canning, Curry, Feist, Longshaw et Okamura, 1999, the causative agent of proliferative kidney disease of salmonids, Here we describe the reaction of mAb B4 against Myxobolus cerebralis Hofer, 1903, the paras...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2004.026
更新日期:2004-06-01 00:00:00
abstract::Parasitological examination of faeces of 26 snakes kept in Bio-Ken Snake Farm, Watamu, Kenya revealed new species of Eimeria Schneider, 1875 in Telescopus semiannulatus Smith, 1849. Oocysts of Eimeria arabukosokokensis sp. n. are cylindrical 26.8 (25-29) x 15.1 (14-16) microm with smooth, bilayered oocyst wall and a s...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2003.004
更新日期:2003-03-01 00:00:00
abstract::The paper contains results of helminthological studies on ruminants introduced into Czechoslovakia from other regions and continents. It is pointed out that introduction of wild ruminants is not only of theoretical importance in the adaptation of parasites to a new environment, but also of practical consequence in the...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Toxoplasmosis is a potentially fatal complication after hematopoietic cell transplantation (HCT). Pre-transplant seropositivity of graft recipient to Toxoplasma gondii (Nicolle et Manceaux, 1908) is an important factor for disease reactivation after HCT. As toxoplasmosis epidemiology varies all over the world, we perf...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2019.019
更新日期:2019-11-11 00:00:00
abstract::Three new species of diphyllidean cestodes are described from stingrays of the genus Pastinachus from the Indian and Pacific Oceans. All three new species differ from all but 10 of the 36 valid species of Echinobothrium van Beneden, 1849 in that they bear lateral hooklets arranged in a continuous band across each late...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2010.025
更新日期:2010-09-01 00:00:00
abstract::Blood films were examined from 154 wild and captive tortoises from four provinces of South Africa, including Gauteng, Kwazulu-Natal, North West and Western Cape. The five species ofchelonians studied were Chersina angulata (Schweigger), Kinixys belliana belliana (Gray), K. lobatsiana Power, K. natalensis Hewitt, and S...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2009.021
更新日期:2009-09-01 00:00:00
abstract::Parasitological examinations of 102 specimens of the lesser great cormorant Phalacrocorax carbo sinensis (Blumenbach) from two nesting regions in the Czech Republic (South Bohemia and South Moravia) were carried out at the Institute of Parasitology, Czech Academy of Sciences (previously the Czechoslovak Academy of Sci...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2016.022
更新日期:2016-06-13 00:00:00
abstract::The structure of legs I and of gnathosoma in Radfordia lemnina (Koch, 1835) and Protomyobia claparedei (Poppe, 1896) is described on the basis of SEM micrographs. ...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::In Italy, food-borne outbreaks of opisthorchiasis have occurred since 2003, all of them originating from some lakes in Central Italy where the only European liver fluke, Opisthorchis felineus (Rivolta, 1884) is endemic. The aim of the present study is to investigate the factors that may facilitate the life cycle of O....
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2018.013
更新日期:2018-09-14 00:00:00
abstract::The developmental cycle of the trematode Rubenstrema exasperatum (Rudolphi, 1819), a common parasite of insectivores of the family Soricidae, is described here for the first time. The first intermediate host in nature is the snail Planorbarius corneus. Metacercariae encysted experimentally in larvae of the mosquito Cu...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::In field and laboratory conditions new data were obtained concerning the life cycle of the nidicolous tick Ixodes laguri. The development of this tick was studied in experimental nests of the European suslik and a two-year cycle of I. laguri was demonstrated. However, the materials obtained indicate that a longer cycl...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Seven species of fishes, Catostomus commersonii (Lacépède), Etheostoma nigrum Rafinesque, Micropterus dolomieu Lacépède, Notemigonus crysoleucas (Mitchill), Notropis hudsonius (Clinton), Perca flavescens (Mitchill), and Percina caprodes (Rafinesque) from the St. Lawrence River, Quebec, Canada, were found infected with...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2006.003
更新日期:2006-03-01 00:00:00
abstract::In Senegal, several areas provide great potential for agriculture and animal production, but African animal trypanosomosis (AAT) is one of the major constraints to the development of more effective livestock production systems. A study was conducted to assess the current situation of AAT in this country. Surveys were ...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2015.044
更新日期:2015-08-18 00:00:00
abstract::We studied the specificity of the individual developmental stages of Fasciola hepatica for evoking immune response of the host to reinfection with this parasite, whereby the primary infection was eliminated by a dose of 150 mg/kg diamphenethide administered in various intervals. In rats we observed a state of hypersen...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The number of peripheral eosinophils was counted in female Swiss albino mice on 1, 4, 9, 16 and 30 days after infection with various single (500, 1000 and 2000) and weekly repeated (500 + + 500 + 1000, 1000 + 1000 and 1000 + 1000 + 2000) doses of filariform Ancylostoma caninum larvae. The eosinophil response was signi...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::The following four species (only females available) of the Philometridae (Nematoda: Dracunculoidea) were recorded from freshwater fishes of Lake Turkana, northwestern Kenya in 2007-2008: Philometra bagri (Khalil, 1965) from the subcutaneous tissue around the mouth, on gill covers and the fin base of the bayad Bagrus b...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2009.008
更新日期:2009-03-01 00:00:00
abstract::A part of the life cycle of Proteocephalus neglectus La Rue, 1911, a parasite of trout, starting from release of eggs from mature parasites into water, to the early phase of development in the definitive host, was studied under experimental conditions. Special regard has been paid to development in the intermediate ho...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Twenty two percent (22/98) of intertidal fishes of 10 species captured in South Africa at Koppie Alleen, De Hoop Nature Reserve (south coast) and Mouille Point, Cape Town (west coast), harboured single or combined infections of haemogregarines, trypanosomes and an intraerythrocytic parasite resembling a Haemohormidium...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2006.031
更新日期:2006-12-01 00:00:00
abstract::The present study evaluates the performance of OptiMAL-IT test and nested PCR assay in detection of malaria parasites. A total of 76 randomly selected blood samples collected from two malaria endemic areas were tested for malaria parasites using microscopy and OptiMAL-IT test in the field. PCR assays were performed in...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2013.041
更新日期:2013-11-01 00:00:00