Abstract：
:The present study evaluates the performance of OptiMAL-IT test and nested PCR assay in detection of malaria parasites. A total of 76 randomly selected blood samples collected from two malaria endemic areas were tested for malaria parasites using microscopy and OptiMAL-IT test in the field. PCR assays were performed in the laboratory using DNA extracted from blood spots of the same samples collected on the FTA classic cards. Of the total of 61 field confirmed malaria positive samples, only 58 (95%) were detected positive using microscopy in the laboratory. Sensitivity, specificity, positive predictive value, negative predictive value and false discovery rate of OptiMal-IT in comparison to the microscopy were 93%, 83%, 95%, 79% and 5%, respectively. On the other hand, the sensitivity and specificity of PCR assay were 97% and 100%, respectively, whereas positive predictive value, negative predictive value and false discovery rate were 100%, 90% and 0%, respectively. The overall performance of OptiMal-IT and PCR assays for malaria diagnosis was 76% and 97%, respectively. PCR assay enabled the identification of infection with Plasmodium malariae Laveran, 1881 in four samples misidentified by microscopy and Plasmodium-specific antigen (PAN) identified by the OptiMAL-IT test. In addition to the standard methods, such PCR assay could be useful to obtain the real incidence of each malaria parasite species for epidemiological perspectives.
journal_name
Folia Parasitol (Praha)journal_title
Folia parasitologicaauthors
Dhiman S,Goswami D,Kumar D,Rabha B,Sharma DK,Bhola RK,Baruah I,Veer Vdoi
10.14411/fp.2013.041subject
Has Abstractpub_date
2013-11-01 00:00:00pages
401-5issue
5eissn
0015-5683issn
1803-6465journal_volume
60pub_type
杂志文章abstract：:Laboratory animals (mice and guinea pigs) were infected with the isolates of Coxiella burnetii (Derrick, 1939) obtained from bovine milk (M18 and M35) and the ticks Ixodes ricinus (Linnaeus, 1758) and Dermacentor marginatus (Sulzer, 1776) (Kl3 and Kl6, respectively), and with the reference strain Nine Mile. Neither mo...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:During the 3rd Czechoslovak mountaineering expedition to the Himalaya in the spring 1973 ticks of the family Ixodidae were collected from small mammals and additionally from pastured sheep, and humans. The tick material represented the following species: Ixodes (P.) ovatus Neumann, 1899, Ixodes (I.) acutitarsus (Karsc...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
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更新日期：2006-12-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2016.013
更新日期：2016-04-20 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2003.018
更新日期：2003-06-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2000.029
更新日期：2000-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2002.051
更新日期：2002-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2008.029
更新日期：2008-09-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2010.008
更新日期：2010-03-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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pub_type： 杂志文章
doi：10.14411/fp.2007.026
更新日期：2007-09-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2013.051
更新日期：2013-11-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2013.012
更新日期：2013-05-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2006.026
更新日期：2006-09-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2015.028
更新日期：2015-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2001.045
更新日期：2001-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The possibility of vertical transmission of Borrelia burgdorferi sensu lato in Ixodes persulcatus Schulze, 1930 ticks was studied in the progeny of 20 females collected from the vegetation in an active focus of ixodid tick-borne borrelioses (ITBB) located in the Perm oblast, Russia, where Borrelia garinii and B. afzel...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2004.010
更新日期：2004-03-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2015.042
更新日期：2015-07-30 00:00:00
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journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Primary amoebic meningoencephalitis (PAM) was induced in mice by intranasal inoculation of Naegleria fowleri (Singh et Das, 1970) to study the role of the blood vessels and lungs in the early and later stages in this disease. Upon culturing blood and lung tissue obtained at 24-, 36-, 48-, 72-, 96-, and 120-hour time p...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2002.035
更新日期：2002-01-01 00:00:00
