Abstract:
:Faecal samples from 162 wild animals were collected from 32 distinct sites of Łęczyńsko-Włodawskie Lakeland (eastern Poland). The presence of Giardia duodenalis (Stiles, 1902) was assessed by a Direct Fluorescence Assay (DFA) and by Polymerase Chain Reaction (PCR) and sequencing of a fragment of the beta-giardin gene. DFA showed the presence of cysts of G. duodenalis in 12 of 162 faecal samples (7%), namely in four wild boars (15%), four foxes (19%), two roe deer (4%), and two wolves (29%). PCR identified 34 of the 162 (21%) samples as positive, including 11 wild boars (41%), five red deer (18%), 11 roe deer (23%), four moose (17%), two wolves (29%) and a single sample from the European badger. Thus, PCR detected a significantly higher number of infection than DFA (P = 0.0005). However, 14 of 34 PCR products could not be sequenced because of their insufficient amount; the low number of cysts, poor conservation of the faeces or presence of PCR inhibitors may have contributed to weak DNA amplification. Sequence analysis of the remaining 20 products showed the presence of assemblage B in wild boars, red deer and roe deer, whereas samples from wolves were identified as assemblage D. This is the first detection of assemblage B in wild boars and deer. As assemblage B has zoonotic potential, wild animals from eastern Poland may act as reservoirs of cysts of G. duodenalis infectious for humans.
journal_name
Folia Parasitol (Praha)journal_title
Folia parasitologicaauthors
Stojecki K,Sroka J,Caccio SM,Cencek T,Dutkiewicz J,Kusyk Pdoi
10.14411/fp.2015.042subject
Has Abstractpub_date
2015-07-30 00:00:00eissn
0015-5683issn
1803-6465journal_volume
62pub_type
杂志文章abstract::The aim of the present study was to estimate the genetic diversity of the cestode Echinococcus multilocularis Leuckart, 1863 in Poland based on sequence analysis of the mitochondrial genes of worms isolated from red foxes, Vulpes vulpes (Linnaeus). Overall, 83 adults of E. multilocularis from the same number of foxes ...
journal_title:Folia parasitologica
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journal_title:Folia parasitologica
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.14411/fp.2003.018
更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2004-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2005-05-01 00:00:00
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更新日期:2014-02-01 00:00:00
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journal_title:Folia parasitologica
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pub_type: 杂志文章
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