Oxidoreduction of different hydroxyl groups in bile acids during their enterohepatic circulation in man.

abstract：:Phosphatidylcholine preparations containing saturated and unsaturated molecular species were subjected to KMnO(4)-NaIO(4) oxidation in aqueous acetic acid, which left only disaturated species intact. After the oxidation, the remaining intact phosphatidylcholine was separated by thin-layer chromatography. The procedure...

journal_title：Journal of lipid research

authors： Shimojo T,Abe M,Ota M

更新日期：1974-09-01 00:00:00

abstract：:Reduced white adipose tissue (WAT) LPL activity delays plasma clearance of TG-rich lipoproteins (TRLs). We reported the secretion of apoC-I, an LPL inhibitor, from WAT ex vivo in women. Therefore we hypothesized that WAT-secreted apoC-I associates with reduced WAT LPL activity and TRL clearance. WAT apoC-I secretion a...

journal_title：Journal of lipid research

authors： Cyr Y,Wassef H,Bissonnette S,Lamantia V,Davignon J,Faraj M

更新日期：2016-06-01 00:00:00

abstract：:The enzymic incorporation of choline-1,2-(14)C from CDP-choline-1,2-(14)C into phosphatidylcholine by spinach leaf preparations was characterized. The enzyme catalyzing the incorporation, choline phosphotransferase, had a pH optimum of about 8.0 and required either Mn(2+) or Mg(2+) as cofactor. The saturation concentr...

journal_title：Journal of lipid research

authors： Devor KA,Mudd JB

更新日期：1971-07-01 00:00:00

abstract：:Native LDL (nLDL) increases expression of adhesion molecules on endothelial cells through induction of Ca(2+) mobilization. Ca(2+) mobilization is also involved in the induction of proinflammatory cytokines, important mediators involved in atherogenesis. The aim of the study was to evaluate the capacity of nLDL to aff...

journal_title：Journal of lipid research

authors： Netea MG,Kullberg BJ,Demacker PN,Jacobs LE,Verver-Jansen TJ,Hijmans A,van Tits LH,Hoenderop JG,Willems PH,Van der Meer JW,Stalenhoef AF

更新日期：2002-07-01 00:00:00

abstract：:Recent evidence suggests the possibility that macrophages can influence lipoprotein metabolism. Therefore we investigated the ability of cultured macrophages to alter low density lipoprotein (LDL) uptake in a human liver cell line (HepG2). Conditioned media from phlogogenic-induced mouse peritoneal macrophages or from...

journal_title：Journal of lipid research

authors： Grove RI,Mazzucco C,Allegretto N,Kiener PA,Spitalny G,Radka SF,Shoyab M,Antonaccio M,Warr GA

更新日期：1991-12-01 00:00:00

abstract：:Three 14C-labeled 22-carbon polyunsaturated fatty acids, 7,10,13,16-[14C]docosatetraenoic acid (22:4(n-6)), 7,10,13,16,19-[14C]docosapentaenoic acid (22:5(n-3)), and 4,7,10,13,16,19-[14C]docosahexaenoic acid (22:6(n-3)), were compared with [3H]arachidonic acid (20:4(n-6] and [14C]linoleic acid (18:2(n-6)) to character...

journal_title：Journal of lipid research

authors： Masuzawa Y,Okano S,Waku K,Sprecher H,Lands WE

更新日期：1986-11-01 00:00:00

abstract：:Several studies indicate that cholesterol esterification is deregulated in cancers. The present study aimed to characterize the role of cholesterol esterification in proliferation and invasion of two tumor cells expressing an activated cholecystokinin 2 receptor (CCK2R). A significant increase in cholesterol esterific...

journal_title：Journal of lipid research

authors： Paillasse MR,de Medina P,Amouroux G,Mhamdi L,Poirot M,Silvente-Poirot S

更新日期：2009-11-01 00:00:00

abstract：:The objective of this study was to investigate the mobilization of individual fatty acids from human subcutaneous adipose tissue in vivo. Concentrations of individual non-esterified fatty acids were measured in arterialized plasma and in the venous drainage from subcutaneous abdominal adipose tissue in eight normal su...

journal_title：Journal of lipid research

authors： Halliwell KJ,Fielding BA,Samra JS,Humphreys SM,Frayn KN

更新日期：1996-09-01 00:00:00

abstract：:As 5-lipoxygenase (5-LO) is an emerging target in obesity and insulin resistance, we have investigated whether this arachidonate pathway is also implicated in the progression of obesity-related fatty liver disease. Our results show that 5-LO activity and 5-LO-derived product levels are significantly elevated in the li...

journal_title：Journal of lipid research

authors： López-Parra M,Titos E,Horrillo R,Ferré N,González-Périz A,Martínez-Clemente M,Planagumà A,Masferrer J,Arroyo V,Clària J

更新日期：2008-12-01 00:00:00

abstract：:In addition to functioning as detergents that aid digestion of dietary lipids in the intestine, some bile acids have been shown to exhibit antimicrobial activity. However, detailed information on the bactericidal activities of the diverse molecular species of bile acid in humans and rodents is largely unknown. Here, w...

journal_title：Journal of lipid research

authors： Watanabe M,Fukiya S,Yokota A

更新日期：2017-06-01 00:00:00

abstract：:Described herein are the chemical syntheses of a number of deuterated derivatives of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one. These include the [2,2,3 alpha,4,4,7,7,9 alpha,16,16-2H10]-, [7 alpha,9 alpha,16,16-2H4]-, [7,7,9 alpha,16,16-2H5]-, and [2,2,3 alpha,4,4-2H5]-analogs of the delta 8(14)-15-ketosterol. Also i...

journal_title：Journal of lipid research

authors： St Pyrek J,Wilson WK,Schroepfer GJ Jr

更新日期：1987-11-01 00:00:00

abstract：:Cholesterol 7alpha-hydroxylase, a rate-limiting enzyme for bile acid synthesis, has been implicated in genetic susceptibility to atherosclerosis. The gene, CYP7A1, encoding a protein with this activity, is expressed normally only in hepatocytes and is highly regulated. Our cyp7A1 gene knockout mouse colony, as young a...

journal_title：Journal of lipid research

authors： Erickson SK,Lear SR,Deane S,Dubrac S,Huling SL,Nguyen L,Bollineni JS,Shefer S,Hyogo H,Cohen DE,Shneider B,Sehayek E,Ananthanarayanan M,Balasubramaniyan N,Suchy FJ,Batta AK,Salen G

更新日期：2003-05-01 00:00:00

abstract：:The enzyme 1-acylglycerol-3-phosphate-O-acyltransferase (AGPAT) converts lysophosphatidic acid (LPA) to phosphatidic acid (PA). In this study, we show enzymatic properties, tissue distribution, and subcellular localization of human AGPAT3 and AGPAT5. In cells overexpressing these isoforms, the proteins were detected i...

journal_title：Journal of lipid research

authors： Prasad SS,Garg A,Agarwal AK

更新日期：2011-03-01 00:00:00

abstract：:Several studies have documented the presence of Lp[a] in nonhuman primates. However, data are lacking in great apes such as the chimpanzee. We have studied the quantitative distribution of Lp[a], as well as the frequency of apo[a] phenotypes, in a population of chimpanzees living in Gabon. Monoclonal antibody 14A12, d...

journal_title：Journal of lipid research

authors： Doucet C,Huby T,Chapman J,Thillet J

更新日期：1994-02-01 00:00:00

abstract：:A new sample preparation method coupled to GC-MS analysis was developed and validated for quantification of sulfate esters of pregnenolone (PREG-S) and dehydroepiandrosterone (DHEA-S) in rat brain. Using a solid-phase extraction recycling protocol, the results show that little or no PREG-S and DHEA-S (<1 pmol/g) is pr...

journal_title：Journal of lipid research

authors： Liere P,Pianos A,Eychenne B,Cambourg A,Liu S,Griffiths W,Schumacher M,Sjövall J,Baulieu EE

更新日期：2004-12-01 00:00:00

abstract：:Lipogenesis in vitro from acetate-1-(14)C was studied in human preputial skin and abdominal skin. Radioactive lipids were separated by column chromatography on Florisil and by thin-layer chromatography on silica gel. Radioactivity was incorporated chiefly into the triglyceride, sterol, and polar lipid fractions, while...

journal_title：Journal of lipid research

authors： Vroman HE,Nemecek RA,Hsia SL

更新日期：1969-09-01 00:00:00

abstract：:The time course of the clearance from the blood and the tissue localization of [14C]L-glucosylceramide, a nonmetabolizable enantiomorph of D-glucosylceramide that accumulates in Gaucher's disease, has been determined. 14C-labeled L-glucosylceramide injected intravenously in the form of micelles or liposomes is rapidly...

journal_title：Journal of lipid research

authors： Tokoro T,Gal AE,Gallo LL,Brady RO

更新日期：1987-08-01 00:00:00

abstract：:The methanolyzed lipids of the extreme halophile, Halobacterium cutirubrum, were separated into glycerol diether and glycerol monoether fractions. The diether was shown by synthesis to be 2,3-di-O-(3'R,7'R,11'R,15'-tetramethylhexadecyl)-sn-glycerol. The monoether fraction was separated by thin-layer chromatography on ...

journal_title：Journal of lipid research

authors： Joo CN,Shier T,Kates M

更新日期：1968-11-01 00:00:00

abstract：:The relationship between the cholesterol 7 alpha-hydroxylase activity and the pool of free cholesterol in rat liver microsomes was studied under experimental conditions aimed to stimulate (biliary drainage, cholestyramine treatment, and lymphatic drainage) as well as inhibit (chenodeoxycholic acid treatment) bile acid...

journal_title：Journal of lipid research

authors： Einarsson K,Akerlund JE,Björkhem I

更新日期：1987-03-01 00:00:00

abstract：:Glycosylphosphatidylinositol (GPI) enriches GPI-anchored proteins (GPI-AP) in lipid rafts by intimate interaction of its lipid moiety with sphingolipids and cholesterol. In addition to such lipid-lipid interactions, it has been reported that GPI may interact with protein moiety linked to GPI and affect protein conform...

journal_title：Journal of lipid research

authors： Seong J,Wang Y,Kinoshita T,Maeda Y

更新日期：2013-04-01 00:00:00

abstract：:Chemokines are a family of cytokines inducing cell migration and inflammation. Recent reports have implicated the roles of chemokines in cell differentiation. However, little is known about the functional roles of chemokines in adipocytes. Here, we explored gene expression levels of chemokines and chemokine receptors ...

journal_title：Journal of lipid research

authors： Kusuyama J,Komorizono A,Bandow K,Ohnishi T,Matsuguchi T

更新日期：2016-10-01 00:00:00

abstract：:It was the aim of this study to i) compare the effects of glucose and other hexoses with that of oleate on secretion of apolipoproteins (apos) A-I and B by HepG2 cells, and ii) document the effect of various metabolic inhibitors on the secretion of these apos in the absence or presence of extra glucose/oleate. i) The ...

journal_title：Journal of lipid research

authors： Kempen HJ,Imbach AP,Giller T,Neumann WJ,Hennes U,Nakada N

更新日期：1995-08-01 00:00:00

abstract：:Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KDS). Here, we report a HPLC-ESI-MS/MS method to directly quantify 3KDS generated by SPT. With this technique, we were able to detect 3KDS at a level comparable to that of...

journal_title：Journal of lipid research

authors： Ren J,Snider J,Airola MV,Zhong A,Rana NA,Obeid LM,Hannun YA

更新日期：2018-01-01 00:00:00

abstract：:Paraoxonase-1 (PON1) is known to be associated with high density lipoproteins. We optimized buffer conditions to obtain quantitative recovery of PON1 (arylesterase) activity and analyzed the distribution of PON1 in mice using a combination of size-exclusion chromatography and ultracentrifugation. Size-exclusion chroma...

journal_title：Journal of lipid research

authors： Connelly PW,Maguire GF,Draganov DI

更新日期：2004-03-01 00:00:00

abstract：:A preparation of cerebral microvessels was used to demonstrate the presence of lipoprotein lipase and acid lipase activity in the microvasculature of rabbit brain. Microvessels, consisting predominantly of capillaries, small arterioles, and venules, were islated from rabbit brain. Homogenates were assayed for lipolyti...

journal_title：Journal of lipid research

authors： Brecher P,Kuan HT

更新日期：1979-05-01 00:00:00

abstract：:Familial hypobetalipoproteinemia, a syndrome associated with low plasma cholesterol levels, can be caused by apoB gene mutations. We identified a healthy 42-year-old man whose total plasma cholesterol level was 80 mg/dl. His plasma very low density lipoprotein (VLDL) contained a unique truncated apoB species, apoB-83,...

journal_title：Journal of lipid research

authors： Farese RV Jr,Garg A,Pierotti VR,Vega GL,Young SG

更新日期：1992-04-01 00:00:00

abstract：:Increased plasma concentrations of apolipoprotein A-IV (apoA-IV) in chronic renal disease suggest a metabolic role of the kidney for this antiatherogenic protein. Therefore, we investigated patients with various forms of proteinuria and found increased serum concentrations of apoA-IV in 124 nephrotic patients compared...

journal_title：Journal of lipid research

authors： Lingenhel A,Lhotta K,Neyer U,Heid IM,Rantner B,Kronenberg MF,König P,von Eckardstein A,Schober M,Dieplinger H,Kronenberg F

更新日期：2006-09-01 00:00:00

abstract：:There is unequivocal evidence of an inverse association between plasma high-density lipoprotein (HDL) cholesterol concentrations and the risk of cardiovascular disease, a finding that has led to the hypothesis that HDL protects from atherosclerosis. This review details the experimental evidence for this "HDL hypothesi...

journal_title：Journal of lipid research

authors： Vergeer M,Holleboom AG,Kastelein JJ,Kuivenhoven JA

更新日期：2010-08-01 00:00:00

abstract：:Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the beta-position on agarose gels. The demonstration of a broad beta-band on agarose gel electrophoresis ...

journal_title：Journal of lipid research

authors： Blom DJ,Byrnes P,Jones S,Marais AD

更新日期：2003-01-01 00:00:00

abstract：:CTP:phosphoethanolamine cytidylyltransferase (Pcyt2) catalyzes the rate-controlling reaction of the CDP-ethanolamine (Kennedy) pathway. We have previously established that Pcyt2 is encoded by a single gene that can be alternatively spliced from an internal exon into two transcripts, designated Pcyt2alpha and Pcyt2beta...

journal_title：Journal of lipid research

authors： Tie A,Bakovic M

更新日期：2007-10-01 00:00:00