Abstract：
:The effect of different levels of mercury substitution on the rate and extent of hybridisation of globin mRNA with a complementary DNA (cDNA) copy has been investigated. It was found that mercuration significantly reduces both the rate of hybridisation and the extent of the reaction, but that these effects are abolished when at least stoichiometric amounts of 2-mercaptoethanol are included in the hybridisation medium. As a preliminary to using this technique to isolate specific groups of sequences after long-term hybridisations, we have investigated both the rate of demercuration of RNA and its retention on thiol-sepharose columns after extended incubation under commonly employed hybridisation conditions at 43 degrees or 60 degrees. Retention was essentially quantitative even after incubation times of 300 hours at 43 degrees, but decreased significantly after 48 hours at 60 degrees. It is concluded that thiol-sepharose chromatography offers considerable advantages over hydroxyapatite chromatography for the recovery of hybridised sequences, particularly with regard to the lower levels of non-specific binding obtained and its ability to distinguish directly between DNA-DNA and DNA-Hg RNA hybrids.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Brown TD,Balmain Adoi
10.1093/nar/7.8.2357subject
Has Abstractpub_date
1979-12-20 00:00:00pages
2357-68issue
8eissn
0305-1048issn
1362-4962journal_volume
7pub_type
杂志文章abstract：:Reverse gyrase is a peculiar DNA topoisomerase, specific of hyperthermophilic Archaea and Bacteria, which has the unique ability of introducing positive supercoiling into DNA molecules. Although the function of the enzyme has not been established directly, it has been suggested to be involved in DNA protection and rep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl115
更新日期：2006-04-14 00:00:00
abstract：:A specific segment of mitochondrial DNA from 18 people was examined by two methods of direct DNA sequencing. This segment includes a small noncoding region (V) shown before by restriction analysis to exhibit length polymorphism. All 11 of the human mtDNAs previously reported to have a deletion in this region proved to...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.2.529
更新日期：1987-01-26 00:00:00
abstract：:A multivariate statistical method called correspondence analysis was used to examine the codon usage of one-hundred-and-one nuclear genes of maize (Zea mays L.). Forty percent of the variation in codon usage was due to bias toward G or C-ending versus A or U-ending codons. Differences in levels of G-ending codons show...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.23.5294
更新日期：1993-11-25 00:00:00
abstract：:Bacteriophage DNA packaging motors translocate their genomic DNA into viral heads, compacting it to near-crystalline density. The Bacillus subtilis phage 29 has a unique ring of RNA (pRNA) that is an essential component of its motor, serving as a scaffold for the packaging ATPase. Previously, deletion of a three-base ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks724
更新日期：2012-10-01 00:00:00
abstract：:Telomeres at the ends of eukaryotic chromosomes are essential for genome integrality and stability. In order to identify genes that sustain telomere maintenance independently of telomerase recruitment, we have exploited the phenotype of over-long telomeres in the cells that express Cdc13-Est2 fusion protein, and exami...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1150
更新日期：2020-12-16 00:00:00
abstract：:Experimental evidence indicates that about 60% of miRNA-binding activity does not follow the canonical rule about the seed matching between miRNA and target mRNAs, but rather a non-canonical miRNA targeting activity outside the seed or with a seed-like motifs. Here, we propose a new unbiased method to identify canonic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx007
更新日期：2017-05-19 00:00:00
abstract：:Microbial enzymes have many known applications as biocatalysts in biotechnology, agriculture, medical and other industries. However, only a few enzymes are currently employed for such commercial applications. In this scenario, the current onslaught of metagenomic data provides a new unexplored treasure trove of genomi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1001
更新日期：2010-01-01 00:00:00
abstract：:The rapidly growing amount of genomic sequence data being generated and made publicly available necessitate the development of new data storage and archiving methods. The vast amount of data being shared and manipulated also create new challenges for network resources. Thus, developing advanced data compression techni...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks709
更新日期：2012-11-01 00:00:00
abstract：:This paper describes a computer program designed to look for similarities between pairs of nucleic or amino acid sequences. The program looks both for segments of perfect identity or for regions where, using a scoring matrix, a minimum value is exceeded. The results of comparisons are presented as a matrix which is di...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.9.2951
更新日期：1982-05-11 00:00:00
abstract：:Binding of the DnaA protein to oriC leads to DNA melting within the DNA unwinding element (DUE) and initiates replication of the bacterial chromosome. Helicobacter pylori oriC was previously identified as a region localized upstream of dnaA and containing a cluster of DnaA boxes bound by DnaA protein with a high affin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks742
更新日期：2012-10-01 00:00:00
abstract：:Various antitumor drugs stabilize DNA topoisomerase II-DNA transient covalent complexes. The complexes distribution along pBR322 DNA was shown previously to depend upon the nature of the drug (Tewey et al. (1984) Science 226, 466-468). The position in pBR322 of DNA cleavage by calf DNA topoisomerase II for 115 such si...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.2861
更新日期：1991-06-11 00:00:00
abstract：:The Mouse Genome Database (MGD) focuses on the integration of mapping, homology, polymorphism and molecular data about the laboratory mouse. Detailed descriptions of genes including their chromosomal location, gene function, disease associations, mutant phenotypes, molecular polymorphisms and links to representative s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.1.95
更新日期：1999-01-01 00:00:00
abstract：:The phenotypic adjustments of Mycobacterium tuberculosis are commonly inferred from the analysis of transcript abundance. While mechanisms of transcriptional regulation have been extensively analysed in mycobacteria, little is known about mechanisms that shape the transcriptome by regulating RNA decay rates. The aim o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz251
更新日期：2019-06-20 00:00:00
abstract：:Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10-20% repeated DNA sequences. There are...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.11.1411
更新日期：1974-11-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression. They are characterized by specific maturation processes defined by canonical and non-canonical biogenic pathways. Analysis of ∼0.5 billion sequences from mouse data sets derived from different tissues, developmental stages and cell types, partly...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1474
更新日期：2013-03-01 00:00:00
abstract：:Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.23.4983
更新日期：1994-11-25 00:00:00
abstract：:Microarrays are an effective tool for monitoring genome-wide gene expression levels. In current microarray analyses, the majority of genes on arrays are frequently eliminated for further analysis because the changes in their expression levels (ratios) are considered to be not significant. This strategy risks failure t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl058
更新日期：2006-03-14 00:00:00
abstract：:Coralyne is a small crescent-shaped molecule known to intercalate duplex and triplex DNA. We report that coralyne can cause the complete and irreversible disproportionation of duplex poly(dT)*poly(dA). That is, coralyne causes the strands of duplex poly(dT)*poly(dA) to repartition into equal molar equivalents of tripl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.4.983
更新日期：2002-02-15 00:00:00
abstract：:The homeobox-containing gene Hex is expressed in several cell types, including thyroid follicular cells, in which it regulates the transcription of tissue- specific genes. In this study the regulation of Hex promoter activity was investigated. Using co- transfection experiments, we demonstrated that the transcriptiona...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg295
更新日期：2003-04-01 00:00:00
abstract：:The C/D box scaRNA2 is predicted to guide specific 2'-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp988
更新日期：2010-01-01 00:00:00
abstract：:TATA-binding protein (TBP) is central to the regulation of eukaryotic transcription initiation. Recruitment of TBP to target genes can be positively regulated by one of two basal transcription factor complexes: SAGA or TFIID. Negative regulation of TBP promoter association can be performed by Mot1p or the NC2 complex....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr784
更新日期：2012-02-01 00:00:00
abstract：:Most small nucleolar RNAs (snoRNAs) guide rRNA nucleotide modifications, some participate in pre-rRNA cleavages, and a few have both functions. These activities involve direct base-pairing of the snoRNA with pre-rRNA using different domains. It is not known if the modification and processing functions occur independen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq043
更新日期：2010-06-01 00:00:00
abstract：:The TWIST1 bHLH transcription factor controls embryonic development and cancer processes. Although molecular and genetic analyses have provided a wealth of data on the role of bHLH transcription factors, very little is known on the molecular mechanisms underlying their binding affinity to the E-box sequence of the pro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw334
更新日期：2016-06-20 00:00:00
abstract：:Mutational analysis has shown that the integrity of the region in domain III of 25S rRNA that is involved in binding of ribosomal protein L25 is essential for the production of mature 25S rRNA in the yeast Saccharomyces cerevisiae. However, even structural alterations that do not noticeably affect recognition by L25, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.24.5001
更新日期：2001-12-15 00:00:00
abstract：:The relationship between the similarity of expression patterns for a pair of genes and interaction of the proteins they encode is demonstrated both for the simple genome of the bacteriophage T7 and the considerably more complex genome of the yeast Saccharomyces cerevisiae. Statistical analysis of large-scale gene expr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.17.3513
更新日期：2001-09-01 00:00:00
abstract：:Insertion/deletion (indel) is one of the most common methods of protein sequence variation. Recent studies showed that indels could affect their flanking regions and they are important for protein function and evolution. Here, we describe the Indel Flanking Region Database (IndelFR, http://indel.bioinfo.sdu.edu.cn), w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1107
更新日期：2012-01-01 00:00:00
abstract：:Adjustable transgene expression is considered key for next-generation molecular interventions in gene therapy scenarios, therapeutic reprogramming of clinical cell phenotypes for tissue engineering and sophisticated gene-function analyses in the post-genomic era. We have designed a portfolio of latest generation self-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh104
更新日期：2004-07-16 00:00:00
abstract：:The integrase (IN) protein of human immunodeficiency virus type 1 (HIV-1) catalyzes site-specific cleavage of 2 bases from the viral long terminal repeat (LTR) sequence yet it binds DNA with little DNA sequence specificity. We have previously demonstrated that the C-terminal half of IN (amino acids 154-288) possesses ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.15.3507
更新日期：1993-07-25 00:00:00
abstract：:The frequencies of each of the 257 468 complete protein coding sequences (CDSs) have been compiled from the taxonomical divisions of the GenBank DNA sequence database. The sum of the codons used by 8792 organisms has also been calculated. The data files can be obtained from the anonymous ftp sites of DDBJ, Kazusa and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.292
更新日期：2000-01-01 00:00:00
abstract：:Chicken erythrocyte inner histones (H2A, H2B, H3 and H4) were associated with the two complementary homopolymeric polydeoxyribonucleotides and the two alternating copolymeric polydeoxyribonucleotides. No evidence for formation of chromatin-like structures was obtained for the complexes with poly(dG) . poly(dC) or poly...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.4.1387
更新日期：1979-04-01 00:00:00
