An enzyme-linked double antibody immunoassay to measure murine immunoglobulins--its application to determine the specific activity of radiolabeled monoclonal antibodies.

abstract：:A rapid method for the simultaneous measurement of neutrophil superoxide generation and beta-glucuronidase release is described. Assay of beta-glucuronidase using a fluorescent substrate is shown to be valid in the presence of reduced or unreduced ferricytochrome C, a prerequisite for the simultaneous assessment of th...

journal_title：Journal of immunological methods

authors： Mandell BF,Ohliger D,Rella J

更新日期：1987-06-26 00:00:00

abstract：:Rhesus monkey serum was applied to a protein A-Sepharose column at pH 7.3. IgG was not detectable in the unbound effluent. Bound protein was eluted with a pH gradient of citrate-phosphate buffer. Two major elution peaks reproducibly resulted at pH 4.85-4.9 and pH 4.35-4.43, respectively, with only slight variability a...

journal_title：Journal of immunological methods

authors： Martin LN

更新日期：1982-01-01 00:00:00

abstract：:Lymphocytes preincubated with various activities of Na251CrO4 were tested for their ability to induce a local, popliteal lymph node graft-versus-host (GVH) reaction. Incubation with 100 micronCi of the isotope per ml of cell suspension impaired the GVH activity, 25 micronCi gave a reduced response in 1 out of 3 experi...

journal_title：Journal of immunological methods

authors： Rolstad B,Toogood E

更新日期：1978-01-01 00:00:00

abstract：:The identification of T cell epitopes that elicit a weak T cell response is technically challenging due to the relatively low resolution of many available screening assays. Peptide immunization can confirm the immunogenicity of a given peptide, however, this also often induces a low frequency response in lymphoid orga...

journal_title：Journal of immunological methods

authors： Usherwood EJ

更新日期：2002-08-01 00:00:00

abstract：:The migration inhibition by soluble PPD of peripheral blood mononuclear cells showed a much better reflection of delayed hypersentitivity to tuberculin in man than the migration inhibition of buffy coat cells. The migration index (MI) with mononuclear cells in a group of tuberculin positive subjects was 0.51 +/- 0.14 ...

journal_title：Journal of immunological methods

authors： Ng RP,Alexopoulos CG

更新日期：1976-01-01 00:00:00

abstract：:Natural killer (NK) cells are spontaneously cytotoxic immune effector cells with the ability to selectively destroy tumor cells without harming normal cells. To perform this function, NK cells utilize two main cytotoxicity pathways, the well known perforin/granzyme-mediated secretory/necrotic killing and the recently ...

journal_title：Journal of immunological methods

authors： Wahlberg BJ,Burholt DR,Kornblith P,Richards TJ,Bruffsky A,Herberman RB,Vujanovic NL

更新日期：2001-07-01 00:00:00

abstract：:An antiglobulin microcytotoxicity assay has been used to analyze non-complement fixing monoclonal antibodies to human histocompatibility antigens. The assay utilizes methodology similar to that of the widely used microcytotoxicity assay for HLA typing, requires low numbers of target cells and is suitable to test large...

journal_title：Journal of immunological methods

authors： Pellegrino M,Russo C,Ng AK,Pellegrino AG,Ferrone S

更新日期：1982-03-12 00:00:00

abstract：:An assay to detect circulating immune complexes is described and exemplified with serum from SLE and RA patients. The method is based on selective precipitation of immune complexes with PEG followed by a specific ELISA assay to detect the immunoglobulins present in the precipitate. The immunoglobulins of the precipita...

journal_title：Journal of immunological methods

authors： Ohlson S,Zetterstrand K

更新日期：1985-02-28 00:00:00

abstract：:The present study describes the immunomagnetic isolation of human natural killer (NK) and lymphokine activated killer (LAK) cells. Antibodies against CD56 and sheep anti-mouse IgG-coated magnetic monodisperse particles (Dynabeads M-450) were used for the positive isolation of CD56+ cells from unstimulated mononuclear ...

journal_title：Journal of immunological methods

authors： Naume B,Nonstad U,Steinkjer B,Funderud S,Smeland E,Espevik T

更新日期：1991-01-24 00:00:00

abstract：:We have developed and validated an inexpensive and equivalent method for measuring eosinophil adhesion by beta(2)-integrin to endothelial ICAM-1 using bovine serum albumin (BSA) as a surrogate for the immunoglobulin supergene. The number of adherent eosinophils on BSA or ICAM-1 coated microplates was quantified by res...

journal_title：Journal of immunological methods

authors： Zhu X,Subbaraman R,Sano H,Jacobs B,Sano A,Boetticher E,Muñoz NM,Leff AR

更新日期：2000-06-23 00:00:00

abstract：:Targeting of antibodies has found a number of applications in assays, anti-idiotypic therapies and vaccine design with a number of anti-idiotypic Abs generated and used in clinical applications, and some currently in clinical trials. Meanwhile, aptamers are a novel and particularly interesting targeting modality, with...

journal_title：Journal of immunological methods

authors： Missailidis S,Thomaidou D,Borbas KE,Price MR

更新日期：2005-01-01 00:00:00

abstract：:A microplate enzyme-linked immunosorbent assay (microELISA) for the study of immunochemical relationships between rabbit myosin light chains is described. Purified individual fast-muscle myosin light chains (LC1F, LC2F and LC3F) and their respective antisera, obtained in chicken, were used. Optimal conditions for anti...

journal_title：Journal of immunological methods

authors： Biral D,Dalla Libera L,Franceschi C,Margreth A

更新日期：1979-01-01 00:00:00

abstract：:Three different methods for the radioiodination of surface proteins in trypanosomatids have been comparatively analyzed. Data interpretation in the lactoperoxidase-mediated method was impaired by autoiodination of the enzyme and its tight retention by the cells. In the enzymobeads method, iodinated enzymes were releas...

journal_title：Journal of immunological methods

authors： Camargo EP,Barbieri CL,Jankevicius JV

更新日期：1982-07-30 00:00:00

abstract：:When limited amounts of antigens are available, it is often difficult to obtain large quantities of polyclonal antisera. Although antisera can be raised in small rodents, yields are usually small. To circumvent these problems, we have designed a modified method for generating polyclonal ascites fluid (AF). Using the a...

journal_title：Journal of immunological methods

authors： Ou SK,Hwang JM,Patterson PH

更新日期：1993-09-27 00:00:00

abstract：:Boronated antibodies have already been evaluated as agents in neutron capture therapy. Because the boronation procedure may alter the properties of the antibody it is important to study the immunoreactivity of the conjugated antibody before in vivo use. In our studies of two dextran-boronated monoclonal antibodies, an...

journal_title：Journal of immunological methods

authors： Pettersson ML,Courel MN,Girard N,Abraham R,Gabel D,Thellier M,Delpech B

更新日期：1990-01-24 00:00:00

abstract：:The advantages of chicken (Gallus gallus domesticus) antibodies as immunodiagnostic and immunotherapeutic biomolecules has only been recently recognized. Even so, chicken antibodies remain less-well characterized than their mammalian counterparts. This review aims at providing a current overview of the structure, func...

journal_title：Journal of immunological methods

authors： Lee W,Syed Atif A,Tan SC,Leow CH

更新日期：2017-08-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) accounts for >700,000 deaths worldwide, largely related to poor rates of diagnosis. Our previous work identified glycoproteins with increased levels of fucosylation in HCC. Plate-based assays to measure this change were compromised by increased levels of heterophilic antibodies with glyc...

journal_title：Journal of immunological methods

authors： Wang M,Shen J,Herrera H,Singal A,Swindell C,Renquan L,Mehta A

更新日期：2018-11-01 00:00:00

abstract：:We describe a rapid in vitro assay for the evaluation of in vivo properties of conjugate vaccines. Using human and murine monoclonal antibodies (mAb) specific for lipopolysaccharides (LPS), isolated from Pseudomonas aeruginosa, we determined in a competitive binding assay the amount of LPS or conjugate vaccine which w...

journal_title：Journal of immunological methods

authors： Bruderer U,Fürer E,Cryz SJ Jr,Lang AB

更新日期：1990-10-19 00:00:00

abstract：:The influence of various methodological variables on the CrCl3 technique for coupling antigen to the surface of erythrocytes has been investigated. Tests should be performed in protein- and phosphate-free medium. The CrCl3 stock solution should be stored at a pH of 5.0 and working dilultions prepared in acetate buffer...

journal_title：Journal of immunological methods

authors： Kofler R,Wick G

更新日期：1977-01-01 00:00:00

abstract：:The methods of cell detachment influence phenotype and function of human macrophages cultured in vitro. However, comparative studies defining the influence of cell detachment techniques on secondary characterization of M1 or M2 polarized macrophages are largely absent from the literature. In this study we evaluated th...

journal_title：Journal of immunological methods

authors： Chen S,So EC,Strome SE,Zhang X

更新日期：2015-11-01 00:00:00

abstract：:This study describes a four-color flow cytometric assay that detects CD4+ T cell responses to the anthrax vaccine. Whole blood from seven volunteers who previously obtained the anthrax vaccine was inoculated in vitro with varying concentrations of the anthrax antigen. TNF-alpha and IFN-gamma production from memory CD4...

journal_title：Journal of immunological methods

authors： Shinn AH,Bravo NC,Maecker HT,Smith JW

更新日期：2003-11-01 00:00:00

abstract：:Bromodeoxyuridine (BrdU) is used to label synthesizing DNA and to chase label-retaining cell (LRC). As stem cells divide slowly in adult tissues, they can be visualized as LRCs. In order to identify LRCs in hard tissue, we examined optimal conditions of fixation, demineralization, and DNA denaturation/antigen retrieva...

journal_title：Journal of immunological methods

authors： Shimada A,Shibata T,Komatsu K,Nifuji A

更新日期：2008-11-30 00:00:00

abstract：:A recombinant retroviral vector with an inserted bacterial neomycin resistance (neo) gene was used to transfer in vitro neomycin resistance (neoR) into murine cytotoxic lymphocyte precursors (CLP). The infection protocol involved co-cultivation of mitogen-activated splenic T-blasts with irradiated cells that produced ...

journal_title：Journal of immunological methods

authors： Reimann J,Heeg K,Wagner H,Keller G,Wagner EF

更新日期：1986-05-01 00:00:00

abstract：:Different Percoll density gradients were used to purify granulocytes from Long-Evans, Fischer, Sprague-Dawley, and Fischer/Long-Evans hybrid rats. Three different discontinuous gradient types were developed which permitted the separation of polymorphonuclear leukocytes (PMNLs) from the peripheral blood of the differen...

journal_title：Journal of immunological methods

authors： Szücs S,Varga C,Ember I,Kertai P

更新日期：1994-01-03 00:00:00

abstract：:A microtechnique to study lymphocyte stimulation in solid cultures by autoradiography is described. Using this technique at a concentration of 2 x 10(6) lymphocytes/chamber, it was found that the percentage of proliferating cells was 8.4% with anti-lymphocyte serum, 3.9% with calcium ionophore A23187, 2.5% with phytoh...

journal_title：Journal of immunological methods

authors： Kondracki E

更新日期：1979-01-01 00:00:00

abstract：:The development of monoclonal antibodies to human differentiation antigens and cytofluorographs have added a new dimension to immune monitoring. However, the technology is such that regionalization will most likely occur. We have demonstrated that human lymphocyte antigens OKT3, OKT4, OKT8 and OKT11 are stable in bloo...

journal_title：Journal of immunological methods

authors： Shield CF 3rd,Marlett P,Smith A,Gunter L,Goldstein G

更新日期：1983-09-16 00:00:00

abstract：:The use of therapeutic antibodies, delivered by intravenous (IV) instillation, is a rapidly expanding area of biomedical treatment for a variety of conditions. However, little is known about how the antibodies are anatomically distributed following infusion and the underlying mechanism mediating therapeutic antibody d...

journal_title：Journal of immunological methods

authors： Schneider JR,Carias AM,Bastian AR,Cianci GC,Kiser PF,Veazey RS,Hope TJ

更新日期：2017-11-01 00:00:00

abstract：:We describe optimal conditions for protein labeling with a new fluorescent probe, 4,7-bis-(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA). The labeled proteins are suitable for time-resolved fluorometric applications because BCPDA forms fluorescent complexes with Eu3+ which exhibit very long fluo...

journal_title：Journal of immunological methods

authors： Diamandis EP,Morton RC

更新日期：1988-08-09 00:00:00

abstract：:Antibody molecules bind to antigen with six complementary determining region (CDR) loops, three of which are located on each variable heavy (V(H)) and light (V(L)) chains. Discovery and optimization of antibodies that bind antigen using in vitro techniques require diversification of one or more of these CDRs. Since an...

journal_title：Journal of immunological methods

authors： Leonard B,Sharma V,Smider V

更新日期：2006-12-20 00:00:00

abstract：:Giant liposomes (mean diameter 5.5 microns) composed of egg phosphatidylcholine or distearoyl phosphatidylcholine, phosphatidyl glycerol, cholesterol and triolein were prepared by a double emulsion technique. They were then mixed with model particulate (killed Bacillus subtilis, and killed Bacille Calmette-Guérin) and...

journal_title：Journal of immunological methods

authors： Antimisiaris SG,Jayasekera P,Gregoriadis G

更新日期：1993-12-03 00:00:00