Abstract：
:The lysosomal endopeptidase cathepsin D is the most abundant proteinase in chondrocytes. Its significance in the pathogenesis of cartilage matrix proteoglycan (PG) degradation in osteoarthritis (OA) is unclear. The extracellular localization of cathepsin D and its potential spatial relationship to areas of PG depletion has been studied in human femoral head OA cartilage. Enzyme was identified by indirect immunofluorescence using rabbit antisera developed against a highly purified cathepsin D preparation. PG distribution was assessed in parallel sections by safranin O staining. Specimens were selected to include regions of cartilage having minimal structural and cellular alterations, severe reduction in thickness, hypocellularity, multicellular chondrocyte clusters and varying degrees of PG loss. Cathepsin D was identified in chondrocytes. When overlying fibrous connective tissue pannus was present, extracellular enzyme was predominantly localized to the cartilage-pannus interface. Cathepsin D could not be demonstrated extracellularly in areas of cartilage that were partially or totally devoid of PG. Chondrocytes in damaged regions, particularly in the superficial and upper transitional zones showing diffuse hypercellularity and/or "brood" clusters, contained increased enzyme staining. Results fail to support a role for cathepsin D in extracellular matrix PG degradation. The potential significance of this enzyme in the pathogenesis of OA would appear relegated to intracellular catabolism. Its intracellular increase at pathologic sites is consistent with enhanced catabolic activity in such regions.
journal_name
Clin Exp Rheumatoljournal_title
Clinical and experimental rheumatologyauthors
Vittorio N,Crissman JD,Hopson CN,Herman JHsubject
Has Abstractpub_date
1986-07-01 00:00:00pages
221-30issue
3eissn
0392-856Xissn
1593-098Xjournal_volume
4pub_type
杂志文章abstract：OBJECTIVES:To compare measurement properties of the EQ-5D and SF-6D utility measures, to assess the association and agreement between these measures and to estimate minimal important differences (MID) in patients with systemic sclerosis (SSc). METHODS:Both measures were assessed twice in an observational prospective d...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Many viruses can be responsible for systemic vasculitis, the most frequent being hepatitis B virus-related polyarteritis nodosa (HBV-PAN), even though its incidence has decreased over the past few decades. Mixed cryoglobulinemia has been shown to be associated with hepatitis C virus (HCV) infection in more than 80% of...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2006-03-01 00:00:00
abstract：OBJECTIVE:To determine the incidence and clinical presentation of Wegener's granulomatosis (WG) in Finland during the 20-year period 1981-2000. METHODS:We performed a study with retrospective data retrieval using the hospital discharge register in Finland. All available hospital case reports were reviewed. We included...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVES:Fibromyalgia is a chronic disorder characterized by widespread musculoskeletal pain and fatigue. Its prevalence is estimated to be at 3.4% in women and 0.5% in men. It is a major cause of morbidity. Our objective was to evaluate, using a self-questionnaire sent by mail, the level of knowledge of French physi...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:The bronchoalveolar lavage (BAL) technique was used to characterize inflammatory cells in the lower respiratory tract of seven nonsmoking patients with CREST syndrome and interstitial pulmonary fibrosis. Differential cell counts in the BAL fluid showed a significant increase of neutrophils (p less than 0.05) and eosin...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：OBJECTIVES:Human six-transmembrane epithelial antigen of prostate 4 (STEAP4) is one of the STEAP family as a homologue of mouse tumour necrosis factor-α-induced adipose-related protein (TIARP). Recently, we reported that the TIARP gene expression was remarkably increased in spleen and joints of glucose-6-phosphate isom...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVES:An educational programme was conducted in Italy in order to favour the diffusion of the rheumatoid arthritis (RA) treat-to-target (T2T) recommendations among Italian rheumatologists. Our objective was to measure the level of acceptance and applicability of the 10 recommendations to treat RA to a target of re...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the relationship between serum infliximab (IFX) levels and changes of RF and ACPA levels in patients with rheumatoid arthritis (RA). METHODS:Enzyme-linked immunosorbent assays (ELISA) [Promonitor® IFX R1 (version 2) (Progenika Biopharma, Spain)] were used to measure ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVES:U1-70K, encoded by the SNRNP70 gene, is a key early immunogen in connective tissue disease. The aim of the study was the genetic analysis of the SNRNP70 gene in mixed connective tissue disease (MCTD), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) patients. METHODS:SNRNP70 genetic variants ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2019-07-01 00:00:00
abstract：:The objectives of this study were: first, to determine the platelet morphology in the peripheral circulation of patients with cyanotic congenital heart diseases and thus its possible link to the development of hypertrophic osteoarthropathy. Second: to test the mathematical model which proposes that in normal individua...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:In terms of adult-onset definitions, rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are rarely diagnosed in children. Adult RA is in most aspects similar to seropositive polyarticular arthritis in children, but AS differs in its clinical presentation according to age at onset. In general, the nomenclature a...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2009-07-01 00:00:00
abstract：:To test whether alternative complement pathway activation explains normal C1s-C1 inhibitor complex in hypocomplementemic (low CH50cl) patients with systemic lupus erythematosus, we examined alternative pathway hemolytic complement (CH50alt) factor B, and Ba fragment in hypocomplementemic sera with normal and with elev...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:To determine the effects of the disease modifying antirheumatic drug (DMARD) leflunomide on the expression of the matrix metalloproteinase MMP-1 (collagenase) and the activity of MMP-9 that are believed to play a major role in cartilage destruction associated with inflammation in patients with rheumatoid arth...
journal_title：Clinical and experimental rheumatology
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2006-03-01 00:00:00
abstract：OBJECTIVE:To investigate the prevalence of antibodies to neuroblastoma cells in patients with rheumatoid arthritis (RA) complicated by peripheral neuropathy (PN), and to determine whether there is any relationship of these antibodies with the severity of neuropathy. METHODS:The study was carried out on 28 patients wit...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Low-dose methotrexate (MTX) remains the first-line treatment option among the disease-modifying antirheumatic drugs in patients with rheumatoid arthritis. However, diverse side effects are the major reason for MTX withdrawal and change of treatment. The following article reviews the published data on general and organ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2010-09-01 00:00:00
abstract：:There is increasing evidence supporting the involvement of Leu 1+B cells in the development of human autoimmune diseases. In this study, the frequency of Leu 1+B cells has been analysed by a multiparameter FACS machine in newborn cord blood, and in the peripheral blood of several autoimmune patients and their healthy ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：OBJECTIVES:Granulomatosis with polyangiitis (GPA) is an autoimmune disease with still unknown etiology. Recent studies indicate that neutrophils extra-cellular traps participate in the pathophysiology of GPA. This study investigates the levels of circulating NET formation markers and neutrophil-platelet interaction in ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：:A 34-year-old woman presented with a right winged scapula 8 months after developing systemic lupus erythematosus (SLE) with subacute cutaneous manifestations. The patient experienced severe shoulder pain followed by weakness of the right arm in the typically winged scapula fashion. Electromyography of the serratus ant...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：OBJECTIVES:To determine the prevalence of juvenile fibromyalgia syndrome (JFMS) in children with familial Mediterranean fever (FMF) and to evaluate quality of life (QoL) and depression. METHODS:Ninety-one FMF patients (M/F: 44/47) who fulfilled the Livneh criteria and 60 healthy children (M/F: 27/33) were enrolled in ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVES:Treatment of rheumatoid arthritis (RA) with disease-modifying anti-rheumatic drugs (DMARDs), either synthetic (sDMARDs) or biologic agents (bDMARDs) has significantly improved disease outcome. However, the impact of therapy-related adverse events (AEs), mild, moderate or serious, on disease outcome is under ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVES:There is a drought of effective treatments of knee osteoarthritis (OA) and new therapies are needed. The present study has been conducted to establish an initial estimate of effectiveness of intra-articular (IA) injection of a proprietary 2.5% cross-linked polyacrylamide hydrogel (PAAG) for the treatment of ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:Methotrexate (MTX) in low doses is widely used in the treatment of rheumatoid arthritis (RA) and it is not known whether its effects are due to immunosuppressive and/or anti-inflammatory actions. High concentrations of MTX inhibit the activity of thymidylate synthetase (TS) and dihydrofolate reductase essenti...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：OBJECTIVES:To assess the clinical spectrum of severe bacterial infections presenting as cutaneous vasculitis (CV) in a defined population. METHODS:Unselected series of 766 patients with CV diagnosed at a single university referral center. RESULTS:An underlying severe bacterial infection was diagnosed in 27 (22 men/5 ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:Patients with rheumatoid arthritis are at greater risk of infectious morbidity and mortality due to disease-related abnormalities and use of immunosuppressive medications. Vaccinations are recommended by international guidelines among infection control strategies, but vaccination rates are reported to be still subopti...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2018-03-01 00:00:00
abstract：:Churg-Strauss syndrome (CSS) is a rare form of systemic vasculitis occurring in patients with asthma. The cause of CSS is unknown, and yet little data are available regarding its pathogenesis. The presence of a marked tissue- and blood-eosinophilia, as well as secretory products of eosinophils in blood and tissues, im...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2003-11-01 00:00:00
abstract：OBJECTIVES:Fibromyalgia syndrome (FM) is a complex disease that is mainly characterised by chronic widespread pain, fatigue and sleep disturbances and may be precipitated or worsened by many stressors. The aim of this study was to observe the behaviour of FM symptoms during the course of coronavirus disease 2019 (COVID...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2020-11-16 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that predominately affects women. It is characterised by a broad spectrum of clinical manifestations, however, its course and organ involvement are unpredictable. Although over the last few decades an improvement in survival for SLE patients has been o...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVES:Autonomic dysfunction (AD) has been described in various chronic inflammatory diseases. Studies of AD in patients with familial Mediterranean fever (FMF) are inconclusive. We aimed to assess AD in a cohort of FMF patients. METHODS:Signs and symptoms of AD were investigated in patients with FMF and compared ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVE:To elucidate the mechanism of gastrointestinal (GI) toxicity of ibuprofen and to examine the effect of altered site of drug release using gastroduodenal and intestinal permeability tests in the rat model. METHODS:Adult male Sprague-Dawley rats were administered (n = 6 per group) either: (1) 100 mg/kg immedia...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Biologic therapies refer to genetically engineered treatments such as monoclonal antibodies and receptor-immunoglobulin fusion proteins. Following many disappointments, the introduction of anti-tumor necrosis factor (anti-TNF alpha) therapies into the clinic has clearly demonstrated the exciting potential of biologic ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1999-11-01 00:00:00
