Abstract:
:Seed germination is a critical stage in the plant life cycle and it plays an important role in the efficiency of agricultural production. However, our knowledge of the mechanisms that regulate seed germination remains limited. In this study, we identified a novel gene, MAPK11, that encodes mitogen-activated protein kinase 11; its expression was significantly higher in seeds of tomato varieties with a low optimum germination temperature than in those with a high optimum germination temperature. In tests at 25 °C, overexpression of MAPK11 in an accession with optimum germination at 25 °C resulted in a decrease in germination, whereas RNAi of MAPK11 in an accession with optimum germination at 15 °C resulted in increased germination. Furthermore, we found that lines overexpressing MAPK11 exhibited hypersensitivity to ABA during germination. These observations were at least partially explained by the fact that MAPK11 up-regulated both NCED1 expression and ABA biosynthesis, and that it also affected ABA signaling and negatively regulated germination by influencing the phosphorylation of SnRK2.2 in vivo. In addition, we found that MAPK11 interacts with and phosphorylates SnRK1 in vivo, thereby potentially inhibiting its activation. SnRK1 interacted with ABI5 and suppressed the transcription of ABI5, thereby affecting ABA signaling and the regulation of germination. Our results demonstrate that ABA signaling in tomato is affected by a mechanism that depends on MAPK11 phosphorylating SnRKs, and this ultimately influences seed germination.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Song J,Shang L,Wang X,Xing Y,Xu W,Zhang Y,Wang T,Li H,Zhang J,Ye Zdoi
10.1093/jxb/eraa564subject
Has Abstractpub_date
2020-12-02 00:00:00eissn
0022-0957issn
1460-2431pii
6015944pub_type
杂志文章abstract::Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the curre...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm254
更新日期:2007-01-01 00:00:00
abstract::FUSCA3 (FUS3) is a short-lived B3-domain transcription factor that regulates seed development and phase transitions in Arabidopsis thaliana. The mechanisms controlling FUS3 levels are currently poorly understood. Here we show that FUS3 interacts with the RING E3 ligase ABI3-INTERACTING PROTEIN2 (AIP2). AIP2-green fluo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx046
更新日期:2017-03-01 00:00:00
abstract::Shoot branching is the process by which axillary buds, located on the axil of a leaf, develop and form new flowers or branches. The process by which a dormant bud activates and becomes an actively growing branch is complex and very finely tuned. Bud outgrowth is regulated by the interaction of environmental signals an...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm134
更新日期:2008-01-01 00:00:00
abstract::Plants respond to many environmental changes by rapidly adjusting their hydraulic conductivity and transpiration rate, thereby optimizing water-use efficiency and preventing damage due to low water potential. A multiple-load-cell apparatus, time-series analysis of the measured data, and residual low-pass filtering met...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq168
更新日期:2010-07-01 00:00:00
abstract::Transcription factors of the NAC family are known to be involved in various developmental processes and in response to environmental stresses. Whereas NAC genes have been widely studied in response to abiotic stresses, little is known about their role in response to biotic stresses, especially in crops. Here, the firs...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert277
更新日期:2013-11-01 00:00:00
abstract::Tobacco plants were transformed by leaf disc regeneration with the VvHT1 (Vitis vinifera hexose transporter 1) cDNA under the control of the constitutive CaMV 35S promoter in a sense or antisense orientation. Among the 20 sense plants and 10 antisense plants obtained, two sense plants showed a mutant phenotype when gr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg119
更新日期:2003-04-01 00:00:00
abstract::Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers120
更新日期:2012-06-01 00:00:00
abstract::This short review outlines the central role of glutamine synthetase (GS) in plant nitrogen metabolism and discusses some possibilities for crop improvement. GS functions as the major assimilatory enzyme for ammonia produced from N fixation, and nitrate or ammonia nutrition. It also reassimilates ammonia released as a ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jexbot/53.370.979
更新日期:2002-04-01 00:00:00
abstract::Rice is an important crop that is very sensitive to salinity. However, some varieties differ greatly in this feature, making investigations of salinity tolerance mechanisms possible. The cultivar Pokkali is salinity tolerant and is known to have more extensive hydrophobic barriers in its roots than does IR20, a more s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err135
更新日期:2011-08-01 00:00:00
abstract::Plant annexins are ubiquitous, soluble proteins capable of Ca(2+)-dependent and Ca(2+)-independent binding to endomembranes and the plasma membrane. Some members of this multigene family are capable of binding to F-actin, hydrolysing ATP and GTP, acting as peroxidases or cation channels. These multifunctional proteins...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm344
更新日期:2008-01-01 00:00:00
abstract::LcbHLH92, a pleiotropic gene from sheepgrass, negatively regulates anthocyanins/proanthocyandins and reduces seed dormancy in transgenic Arabidopsis. ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery335
更新日期:2019-01-01 00:00:00
abstract::The effects of synchronous photo (16 h daylength) and thermo (2 degrees C daily fluctuation) cycles on flowering time were compared with constant light and temperature treatments using two barley mapping populations derived from the facultative cultivar 'Dicktoo'. The 'Dicktoo'x'Morex' (spring) population (DM) segrega...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern131
更新日期:2008-01-01 00:00:00
abstract::The effect of the complex relationship between ethylene and abscisic acid (ABA) on flower development and senescence in Hibiscus rosa-sinensis L. was investigated. Ethylene biosynthetic (HrsACS and HrsACO) and receptor (HrsETR and HrsERS) genes were isolated and their expression evaluated in three different floral tis...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err218
更新日期:2011-11-01 00:00:00
abstract::Carnivorous plants inhabit nutrient-poor environments, where prominent targets of prey capture are organic nitrogen and phosphorus. Some carnivorous plants also acquire carbon from their victims. A new report focusing on Utricularia, the bladderwort, demonstrates that carbon assimilated from photosynthesis is paradoxi...
journal_title:Journal of experimental botany
pub_type: 评论,杂志文章
doi:10.1093/jxb/erp349
更新日期:2010-01-01 00:00:00
abstract::Water is a principal limitation to agricultural production during drought and in arid regions of the world. Mechanisms that plants use to cope with drought can be grouped into two different strategies: drought tolerance and drought avoidance. Previous efforts toward engineering plants for improved performance during d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.369.699
更新日期:2002-04-01 00:00:00
abstract::Cytoplasmic male sterile (CMS) rice has been widely used for hybrid rice seed production in China. However, CMS rice suffers from undesirable flowering habits including scattered floret opening time (FOT), which causes different FOTs among parental rice plants and greatly reduces hybrid rice seed production. Little is...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx251
更新日期:2017-07-20 00:00:00
abstract::Xylans are the most abundant non-cellulosic polysaccharide found in plant cell walls. A diverse range of xylan structures influence tissue function during growth and development. Despite the abundance of xylans in nature, details of the genes and biochemical pathways controlling their biosynthesis are lacking. In this...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw424
更新日期:2016-12-01 00:00:00
abstract::Nine Gretchen Hagen (GH3) genes were identified in grapevine (Vitis vinifera L.) and six of these were predicted on the basis of protein sequence similarity to act as indole-3-acetic acid (IAA)-amido synthetases. The activity of these enzymes is thought to be important in controlling free IAA levels and one auxin-indu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err134
更新日期:2011-08-01 00:00:00
abstract::The molecular mechanisms of abscisic acid (ABA) signalling have been studied for many years; however, how mitochondria-localized proteins play roles in ABA signalling remains unclear. Here an Arabidopsis mitochondria-localized protein RRL (RETARDED ROOT GROWTH-LIKE) was shown to function in ABA signalling. A previous ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv356
更新日期:2015-10-01 00:00:00
abstract::Auxin is an essential regulator of plant growth and development, and auxin signaling components are conserved among land plants. Yet, a remarkable degree of natural variation in physiological and transcriptional auxin responses has been described among Arabidopsis thaliana accessions. As intraspecies comparisons offer...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw457
更新日期:2017-01-01 00:00:00
abstract::Pepper (Capsicum annuum L.) and tomato (Solanum lycopersicum L.), which belong to the Solanaceae family, are among the most cultivated and consumed fleshy fruits worldwide and constitute excellent sources of many essential nutrients, such as vitamins A, C, and E, calcium, and carotenoids. While fruit ripening is a hig...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erx453
更新日期:2018-06-19 00:00:00
abstract::The activity of ionically bound peroxidases from an asparagus spear increased from 5-24 h post-harvest. Isoelectric focusing showed that the post-harvest increase of the total peroxidase activity was due to the increase of several distinct isoperoxidases. Concomitantly, a decrease in the activity of two anionic peroxi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg253
更新日期:2003-10-01 00:00:00
abstract::Although plants are photoautotrophic organisms, they are composed of many heterotrophic tissue systems that must import sugars and amino acids in a process known as assimilate partitioning. While the general features of assimilate partitioning were well described by the early 1980s, little was known about the various ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/47.Special_Issue.1205
更新日期:1996-08-01 00:00:00
abstract::Sexual reproduction in higher plants uses pollination, involving interactions between pollen and pistil. Self-incompatibility (SI) prevents self-fertilization, providing an important mechanism to promote outbreeding. SI is controlled by the S-locus; discrimination occurs between incompatible pollen, which is rejected,...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm195
更新日期:2008-01-01 00:00:00
abstract::Flowering Locus C (FLC) is a key floral repressor that precisely controls flowering time. The role of FLC has been extensively studied at the transcriptional level using molecular biological and epigenetic approaches. However, how FLC functions and how its stability is controlled at the post-translational level are on...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw431
更新日期:2017-01-01 00:00:00
abstract::Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers175
更新日期:2012-09-01 00:00:00
abstract::Diacylglycerol acyltransferases (DGATs) catalyse the final step of the triacylglycerol (TAG) biosynthesis of the Kennedy pathway. Two major gene families have been shown to encode DGATs, DGAT1 (type-1) and DGAT2 (type-2). Both genes encode membrane-bound proteins, with no sequence homology to each other. In this study...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq286
更新日期:2011-01-01 00:00:00
abstract::Plant-atmosphere NH(3) exchange was studied in white clover (Trifolium repens L. cv. Seminole) growing in nutrient solution containing 0 (N(2) based), 0.5 (low N) or 4.5 (high N) mM NO(3)(-). The aim was to show whether the NH(3) exchange potential is influenced by the proportion of N(2) fixation relative to NO(3)(-) ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Branching is an important trait of plant development regulated by environmental signals. Phytochromes in Arabidopsis mediate branching in response to the changes in the red light:far-red light ratio (R:FR), the mechanisms of which are still elusive. Here it is shown that overexpression of CONSTANS-LIKE 7 (COL7) result...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers376
更新日期:2013-02-01 00:00:00
abstract::Teosinte branched1/cycloidea/proliferating (TCP) transcription factors play a broad role in plant growth and development, but their involvement in the regulation of anthocyanin biosynthesis is currently unclear. In this study, anthocyanin biosynthesis induced by different light intensities in apple (Malus domestica) w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa056
更新日期:2020-05-30 00:00:00