Abstract:
OBJECTIVES:This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration. METHODS:0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydration method. Release of TGF-β1 from the liposomes was quantified by an enzyme-linked immunosorbent assay (ELISA). The hDPSCs were treated with different concentrations of liposomal TGF-β1 and cell viability was tested using an MTT assay. "Osteodentine" differentiation capacity was assessed by RT-qPCR, ELISA and Alizarin red S staining. RESULTS:The ELISA results showed that liposomal TGF-β1 achieved a controlled and prolonged release over time. The MTT results demonstrated that the liposomes (100 μg/mL) were not cytotoxic to the cells. Liposomal TGF-β1 up-regulated the expression of "osteodentine" markers, RUNX-2, DMP-1 and DSPP, in hDPSCs after 7 days of treatment and resulted in the accumulation of mineralised nodules. CONCLUSION:This study indicated that liposomes are an effective carrier for delivering TGF-β1 over time. Liposomal TGF-β1 promoted dentinogenesis and increased mineralisation in hDPSCs. This highlights the potential of liposomal TGF-β1 for future use in dentine-pulp regeneration. CLINICAL SIGNIFICANCE:Liposomal TGF-β1 may be used as a synergist for promoting dentine-pulp regeneration of immature permanent teeth or as a pulp capping agent for inducing reparative dentine formation.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Jiang L,Ayre WN,Melling GE,Song B,Wei X,Sloan AJ,Chen Xdoi
10.1016/j.jdent.2020.103501subject
Has Abstractpub_date
2020-12-01 00:00:00pages
103501eissn
0300-5712issn
1879-176Xpii
S0300-5712(20)30248-7journal_volume
103pub_type
杂志文章abstract:OBJECTIVES:To evaluate the efficacy of different bleaching systems on artificially created stained-remineralized caries lesions; and to assess the susceptibility of the bleached lesions to further demineralization. METHODS:Human enamel specimens were sectioned, polished, demineralized, and randomly divided into six gr...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2018.12.008
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVES:To evaluate the toothbrush wear resistance of infiltrated artificial white spot lesions following two infiltration strategies, and to assess their ultramorphology. METHODS:Flat enamel surfaces from freshly extracted bovine teeth were polished and immersed in a Buskes demineralising solution for 30 days to c...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.02.009
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVES:This paper reports the findings of a study on the roles of dental nurses in cross-infection control in general dental practices and who were undergoing training for their initial certificate. The research aimed to find out what factors in their vocation gave them satisfaction, what caused stress and the bear...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(95)00094-1
更新日期:1996-09-01 00:00:00
abstract:OBJECTIVES:The objectives of this study were to investigate: (1) the antibacterial activity of two antibacterial monomers, dimethylaminododecyl methacrylate (DMADDM) and dimethylammoniumethyl dimethacrylate (DMAEDM), against eight different species of oral pathogens for the first time; (2) the cytotoxicity of DMAEDM an...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2013.07.006
更新日期:2013-10-01 00:00:00
abstract::Replacement of pin-retained amalgam restorations is complicated by the loss of prime pin sites and remaining pin sites posing anatomical problems. Self-threading dentine pins can be removed with an ultrasonic handpiece, and the pin site reused with a large pin. Retention of replacement pins is similar to original pins...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(93)90109-4
更新日期:1993-10-01 00:00:00
abstract:OBJECTIVES:The purpose of the study was to evaluate gloss and color changes of resin composites after exposure to different bleaching agents. METHODS:Products used, were: Nite White ACP (10% carbamide peroxide gel), Crest Classic and Supreme Whitestrip (6.5% and 14% hydrogen peroxide strips, accordingly). A hybrid (He...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2010.06.006
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:The purpose of the current study was to examine whether alumina abrasion regime influenced the necessary surface roughness conducive to mechanical interlocking prior to composite resin bonding. The impact of alumina abrasion and cement lute on the bi-axial flexure strength and associated Weibull Moduli (m) o...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2003.08.004
更新日期:2004-01-01 00:00:00
abstract::The polymeric materials currently used in GIC are based on poly(acrylic acid), poly(acrylic acid-co-itaconic acid), or poly(acrylic acid-co-maleic acid). For the visible light cured (VLC) type GIC, the polymeric material is chemically modified to have pendant free-radical polymerizable double bonds, with the aqueous f...
journal_title:Journal of dentistry
pub_type: 杂志文章,评审
doi:10.1016/j.jdent.2005.08.008
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the influence of shortening the etching time on the bond strength of a conventional and a self-etching primer adhesive system used in primary tooth dentin. METHODS:Flat dentin surfaces were obtained from 24 primary molars, randomly assigned to 4 experimental groups. T...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2004.10.011
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVE:Following a successful 2005-2012 phase with three regional practice-based research networks (PBRNs), a single, unified national network called "The National Dental PBRN" was created in 2012 in the United States to improve oral health by conducting practice-based research and serving dental professionals throu...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2013.04.002
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVES:This longitudinal randomized controlled clinical trial evaluated the longevity of composite resin inlays in single- or multi-surface cavities up to 4 years. METHODS:21 dental students placed 75 Artglass and 80 Charisma composite resin inlays in class I and II cavities in posterior teeth (89 adult patients) ...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2011.04.005
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:The light intensity of curing lights used in private dental offices was measured using commercial curing and heat radiometers and related to uniformity of cure depth of standardized composite specimens. METHODS:The intensity of 130 curing light from 107 dental offices was measured with curing and heat radio...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(98)00052-9
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVES:The tooth-resin bond is the weak link of restoration, with secondary caries as a main reason for failure. Calcium phosphate-containing resins are promising for remineralization; however, calcium (Ca) and phosphate (P) ion releases last only a couple of months. The objectives of this study were to develop the...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2015.06.009
更新日期:2015-12-01 00:00:00
abstract::Tooth wear attributed to the physical wear of teeth by clenching and grinding (attrition) has been described previously in alcoholic patients. However, the pattern of wear seen in the series of cases reported here is more consistent with chemical damage (erosion) than attrition. A possible mechanism for this process i...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(89)90168-1
更新日期:1989-10-01 00:00:00
abstract:OBJECTIVES:The goal of this study was to evaluate influence of different shade guides and respective shade matching methods on shade matching results and to analyze user satisfaction with these products and methods. METHODS:Eighty-eight pre-doctoral dental students completed the study. The color of four target shade t...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2009.02.005
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:To validate a new questionnaire for evaluating the 'Oral aesthetic-related quality of life (OARQoL)' of prosthetically restored patients. 'OARQoL' assesses the impact of the self-perceived dental aesthetics on patients' well-being. METHODS:The 'Quality of Life associated with Dental Aesthetic Satisfaction (...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2015.08.010
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVES:Full-ceramic systems can be produced by different techniques (layering, heatpressing, CAD/CAM) and have various compositions with different crystalline contents that may affect the optical properties of laminate restorations. METHODS:A total of 60 specimens were prepared from e.max Press, e.max CAD, Empress...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2012.11.013
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:The main objective is the characterisation of the setting reaction in glass ionomer cements based on experimental ionomer glasses with different fluorine content and a commercial glass ionomer cement liquid by using 13C CP/MAS-NMR, 29Si, 27Al and 31P MAS-NMR spectroscopy in order to receive information speci...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2005.08.006
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVES:This meta-analysis was performed to evaluate the efficacy of potassium nitrate and sodium fluoride as desensitizing agents during tooth bleaching treatment. DATA, SOURCES AND STUDY SELECTION:An electronic systematic literature search was conducted in Cochrane Center Register of Controlled Trials, MEDLINE (P...
journal_title:Journal of dentistry
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.jdent.2015.03.015
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVES:The aim of the present study is to investigate variables of the past caries experience as indicators of future caries in a Chinese child population. METHODS:Caries was recorded at baseline and 2 years later in 433, 6-7-year-old Chinese children. Correlation coefficients between variables of the past caries ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2005.12.010
更新日期:2006-10-01 00:00:00
abstract::Enamel blocks bearing artificial caries are used in intra-oral appliances for cariogenicity tests. These blocks are often sterilised to prevent the possibility of cross-infection via this route. This study therefore aimed to determine the effect of sterilisation methods on the structural integrity of artificial enamel...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(98)00064-5
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVES:To determine the visual 50:50% acceptability thresholds for lightness, chroma and hue for dental ceramics using CIEDE2000(K(L):K(C):K(H)) formula, and to evaluate the formula performance using different parametric factors. METHODS:A 30-observer panel evaluated three subsets of ceramic samples: lightness sub...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.09.007
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVES:This clinical study evaluated the long-term outcome and survival rate of all-ceramic cantilever resin-bonded fixed dental prostheses (RBFDPs) made of zirconia-ceramic with a single-retainer design to replace missing incisors. In addition, whether the reason for missing incisors has an influence on the longev...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.07.003
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:Evaluate the dentine bond strength using different methods of application of opaque resins to mask dentine discoloration. MATERIALS AND METHODS:Dentine was exposed on 21 extracted molars which were assigned to 3 groups: G1: immediate dentine sealing (IDS). G2: IDS+opaque. G3: IDS with adhesive resin mixed with...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.07.005
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:To investigate the wear mechanisms and evolution of damage in tooth enamel-resin composite bonded interfaces caused by sliding contact, and to develop an understanding of interface degradation from a tribological viewpoint that supports clinical recommendations for improving interface integrity. METHODS:Reci...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2019.02.004
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVE:To evaluate a novel methodology using industrial scanners as a reference, and assess in vivo accuracy of 3 intraoral scanners (IOS) and conventional impressions. Further, to evaluate IOS precision in vivo. METHODS:Four reference-bodies were bonded to the buccal surfaces of upper premolars and incisors in fiv...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.12.006
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:To evaluate bone reconstruction and soft tissue reactions at immediate implants placed into intact sockets and those with buccal bone dehiscence defects. METHODS:Fifty-nine internal connection implants from four different manufacturers were immediately placed in intact sockets(non-dehiscence group, n=40), a...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.07.010
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVES:To compare the clinical performance of Scotchbond™ Universal Adhesive used in self- and total-etch modes and two-bottle Scotchbond™ Multi-purpose Adhesive in total-etch mode for Class 5 non-carious cervical lesions (NCCLs). METHODS:37 adults were recruited with 3 or 6 NCCLs (>1.5mm deep). Teeth were isolate...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2015.07.009
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare four finishing/polishing systems (F/P) on surface roughness and gloss of different resin composites. METHODS:A total of 40 disc samples (15 mm × 3 mm) were prepared from a nanofill - Filtek Supreme Plus (FS) and a micro-hybrid resin composite - Esthet-X (EX). Following 2...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.01.006
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:The objectives of this study were two-fold: (1) to determine (by surfometry) loss of deciduous and permanent enamel and dentine following 15days' exposure to a single low pH orange drink; and (2) to determine (by surfometry) loss of deciduous and permanent enamel and dentine following exposure to the product...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(99)00074-3
更新日期:2000-05-01 00:00:00