Variability in haemoglobin concentration by measurement tool and blood source: an analysis from seven countries.

Abstract:

OBJECTIVE:We explore factors such as the blood sampling site (capillary vs venous), the equipment (HemoCue vs automated haematology analyser) and the model of the HemoCue device (201+ vs 301) that may impact haemoglobin measurements in capillary and venous blood. METHODS:Eleven studies were identified, and bias, concordance and measures of diagnostic performance were assessed within each study. FINDINGS:Our analysis included 11 studies from seven countries (Cambodia, India, The Gambia, Ghana, Laos, Rwanda and USA). Samples came from children, men, non-pregnant women and pregnant women. Mean bias ranged from -8.7 to 2.5 g/L in Cambodian women, 6.2 g/L in Laotian children, 2.4 g/L in Ghanaian women, 0.8 g/L in Gambian children 6-23 months and 1.4 g/L in Rwandan children 6-59 months when comparing capillary blood on a HemoCue to venous blood on a haematology analyser. Bias was 8.3 g/L in Indian non-pregnant women and 2.6 g/L in Laotian children and women and 1.5 g/L in the US population when comparing capillary to venous blood using a HemoCue. For venous blood measured on the HemoCue compared with the automated haematology analyser, bias was 5.3 g/L in Gambian pregnant women 18-45 years and 11.3 g/L in Laotian children 6-59 months. CONCLUSION:Our analysis found large variability in haemoglobin concentration measured on capillary or venous blood and using HemoCue Hb 201+ or Hb 301 or automated haematology analyser. We cannot ascertain whether the variation is due to differences in the equipment, differences in capillary and venous blood, or factors affecting blood collection techniques.

journal_name

J Clin Pathol

authors

Rappaport AI,Karakochuk CD,Hess SY,Whitehead RD Jr,Namaste SML,Dary O,Parker ME,Neufeld LM,Larson LM,Newton S,Wegmuller R,Moorthy D

doi

10.1136/jclinpath-2020-206717

subject

Has Abstract

pub_date

2020-10-06 00:00:00

eissn

0021-9746

issn

1472-4146

pii

jclinpath-2020-206717

pub_type

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