Abstract:
:Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9-83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Xing S,Chen K,Zhu H,Zhang R,Zhang H,Li B,Gao Cdoi
10.1186/s13059-020-02146-5subject
Has Abstractpub_date
2020-09-03 00:00:00pages
230issue
1eissn
1474-7596issn
1474-760Xpii
10.1186/s13059-020-02146-5journal_volume
21pub_type
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