Abstract:
:Keith Joung, Dan Voytas and Joanne Kamens share insights into how the genome editing field was advanced by early access to biological resources and the role in this process that plasmid repositories play.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Joung JK,Voytas DF,Kamens Jdoi
10.1186/s13059-015-0830-ysubject
Has Abstractpub_date
2015-11-20 00:00:00pages
255eissn
1474-7596issn
1474-760Xpii
10.1186/s13059-015-0830-yjournal_volume
16pub_type
面试相关文献
GENOME BIOLOGY文献大全abstract:BACKGROUND:The lifelong accumulation of somatic mutations underlies age-related phenotypes and cancer. Mutagenic forces are thought to shape the genome of aging cells in a tissue-specific way. Whole genome analyses of somatic mutation patterns, based on both types and genomic distribution of variants, can shed light on...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-019-1892-z
更新日期:2019-12-18 00:00:00
abstract::High-throughput technologies have enabled the systematic identification and characterization of most, or possibly all, of the components governing segmentation in the fruit fly Drosophila. What have we learned? ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2008-9-2-207
更新日期:2008-01-01 00:00:00
abstract::Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-016-1082-1
更新日期:2016-10-31 00:00:00
abstract::We have developed a new strategy for de novo prediction of splice junctions in short-read RNA-seq data, suitable for detection of novel splicing events and chimeric transcripts. When tested on mouse RNA-seq data, >31,000 splice events were predicted, of which 88% bridged between two regions separated by journal_title:Genome biology pub_type: 杂志文章 doi:10.1186/gb-2010-11-3-r34 更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:DNA replication plays an important role in mutagenesis, yet little is known about how it interacts with other mutagenic processes. Here, we use somatic mutation signatures-each representing a mutagenic process-derived from 3056 patients spanning 19 cancer types to quantify the strand asymmetry of mutational ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-018-1509-y
更新日期:2018-09-10 00:00:00
abstract::Two landmark studies of cell signaling, by RNA interference and phosphoproteomics, provide complementary global views of the pathways downstream of receptor kinases, including those regulated by Erks. ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2007-8-1-202
更新日期:2007-01-01 00:00:00
abstract::Ever since DNA microarrays were first applied to the quantitation of RNA levels, there has been considerable interest in generating a protein homolog that can be used to assay cellular protein expression. A recent paper describes the first microarray that can be used for such protein profiling. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-2-reviews1004
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Francisella tularensis subspecies tularensis and holarctica are pathogenic to humans, whereas the two other subspecies, novicida and mediasiatica, rarely cause disease. To uncover the factors that allow subspecies tularensis and holarctica to be pathogenic to humans, we compared their genome sequences with t...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2007-8-6-r102
更新日期:2007-01-01 00:00:00
abstract::Field pathogenomics adds highly informative data to surveillance surveys by enabling rapid evaluation of pathogen variability, population structure and host genotype. ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-015-0615-3
更新日期:2015-03-02 00:00:00
abstract::Toxin/antitoxin (TA) systems, viewed as essential regulators of growth arrest and programmed cell death, are widespread among prokaryotes, but remain sparsely annotated. We present RASTA-Bacteria, an automated method allowing quick and reliable identification of TA loci in sequenced prokaryotic genomes, whether they a...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2007-8-8-r155
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about their role in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become usefu...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-017-1368-y
更新日期:2017-12-20 00:00:00
abstract:BACKGROUND:Alcoholism presents widespread social and human health problems. Alcohol sensitivity, the development of tolerance to alcohol and susceptibility to addiction vary in the population. Genetic factors that predispose to alcoholism remain largely unknown due to extensive genetic and environmental variation in hu...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2006-7-10-r95
更新日期:2006-01-01 00:00:00
abstract::We present a graph-based method for the analysis of repeat families in a repeat library. We build a repeat domain graph that decomposes a repeat library into repeat domains, short subsequences shared by multiple repeat families, and reveals the mosaic structure of repeat families. Our method recovers documented mosaic...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2006-7-1-r7
更新日期:2006-01-01 00:00:00
abstract::Large-scale biology among plant species, as well as comparative genomics of circadian clock architecture and clock-regulated output processes, have greatly advanced our understanding of the endogenous timing system in plants. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2013-14-6-208
更新日期:2013-06-24 00:00:00
abstract::A new study shows how a single cytokine, interleukin-4, regulates hematopoietic lineage choice by activating the JAK3-STAT6 pathway, which causes dendritic-cell-specific DNA demethylation. ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-016-0884-5
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:DNA methylation is a key epigenetic mechanism for driving and stabilizing cell-fate decisions. Local deposition and removal of DNA methylation are tightly coupled with transcription factor binding, although the relationship varies with the specific differentiation process. Conversion of monocytes to osteocla...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2013-14-9-r99
更新日期:2013-01-01 00:00:00
abstract::In most eukaryotes, histones, which are the major structural components of chromatin, are expressed as a family of sequence variants encoded by multiple genes. Because different histone variants can contribute to a distinct or unique nucleosomal architecture, this heterogeneity can be exploited to regulate a wide rang...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-7-reviews0006
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Despite decades of research, the agent responsible for transmitting spongiform encephalopathies (TSEs) has not been identified. The Prion hypothesis, which dominates the field, supposes that modified host PrP protein, termed PrPSc, acts as the transmissible agent. This model fits the observation that TSE dis...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2001-2-7-preprint0006
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:We have constructed Bayesian prior-based, amino-acid sequence profiles for the complete yeast mitochondrial proteome and used them to develop methods for identifying and characterizing the context of protein mutations that give rise to human mitochondrial diseases. (Bayesian priors are conditional probabilit...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2001-2-6-research0021
更新日期:2001-01-01 00:00:00
abstract::Gene regulatory networks (GRNs) are rapidly being delineated, but their quality and biological meaning are often questioned. Here, I argue that biological meaning is challenging to define and discuss reasons why GRN validation should be interpreted cautiously. ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2011-12-4-109
更新日期:2011-01-01 00:00:00
abstract::A report of the 6th Georgia Tech-Oak Ridge National Lab International Conference on Bioinformatics 'In silico Biology: Gene Discovery and Systems Genomics', Atlanta, USA, 15-17 November, 2007. ...
journal_title:Genome biology
pub_type:
doi:10.1186/gb-2008-9-2-302
更新日期:2008-01-01 00:00:00
abstract::We demonstrate that loss-of-function yeast phenotypes are predictable by guilt-by-association in functional gene networks. Testing 1,102 loss-of-function phenotypes from genome-wide assays of yeast reveals predictability of diverse phenotypes, spanning cellular morphology, growth, metabolism, and quantitative cell sha...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2007-8-12-r258
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The mobilization of transposable elements (TEs) is suppressed by host genome defense mechanisms. Recent studies showed that the cis-regulatory region of Arabidopsis thaliana COPIA78/ONSEN retrotransposons contains heat-responsive elements (HREs), which cause their activation during heat stress. However, it r...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/s13059-016-1072-3
更新日期:2016-10-11 00:00:00
abstract::MAP1-family proteins are classical microtubule-associated proteins (MAPs) that bind along the microtubule lattice. The founding members, MAP1A and MAP1B, are predominantly expressed in neurons, where they are thought to be important in the formation and development of axons and dendrites. Mammalian genomes usually con...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2006-7-6-224
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:RNA-binding proteins regulate a number of cellular processes, including synthesis, folding, translocation, assembly and clearance of RNAs. Recent studies have reported that an unexpectedly large number of proteins are able to interact with RNA, but the partners of many RNA-binding proteins are still uncharac...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2014-15-1-r13
更新日期:2014-01-02 00:00:00
abstract:BACKGROUND:Growth rate is central to the development of cells in all organisms. However, little is known about the impact of changing growth rates. We used continuous cultures to control growth rate and studied the transcriptional program of the model eukaryote Saccharomyces cerevisiae, with generation times varying be...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2006-7-11-r107
更新日期:2006-01-01 00:00:00
abstract::Genomic and proteomic studies have identified hundreds of proteins from mitochondria. A recent study has added a functional twist to these systematic approaches and identified novel mitochondrial modifiers and regulators. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2008-9-2-209
更新日期:2008-01-01 00:00:00
abstract::Each human has approximately 50 to 280 frameshifting indels, yet their implications are unknown. We created SIFT Indel, a prediction method for frameshifting indels that has 84% accuracy. The percentage of human frameshifting indels predicted to be gene-damaging is negatively correlated with allele frequency. We also ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2012-13-2-r9
更新日期:2012-02-09 00:00:00
abstract:BACKGROUND:Hemiascomycetous yeasts have intron-poor genomes with very few cases of alternative splicing. Most of the reported examples result from intron retention in Saccharomyces cerevisiae and some have been shown to be functionally significant. Here we used transcriptome-wide approaches to evaluate the mechanisms u...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2010-11-6-r65
更新日期:2010-01-01 00:00:00
abstract::A report on the Epigenetic Engineering Meeting hosted by the Barts Institute of Cancer, held in London, UK, May 7, 2014. ...
journal_title:Genome biology
pub_type:
doi:10.1186/gb4179
更新日期:2014-01-01 00:00:00