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Minimal genome-wide human CRISPR-Cas9 library.

Abstract：

:CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.

journal_name

Genome Biol

journal_title

Genome biology

authors

Gonçalves E,Thomas M,Behan FM,Picco G,Pacini C,Allen F,Vinceti A,Sharma M,Jackson DA,Price S,Beaver CM,Dovey O,Parry-Smith D,Iorio F,Parts L,Yusa K,Garnett MJ

doi

10.1186/s13059-021-02268-4

subject

Has Abstract

pub_date

2021-01-21 00:00:00

pages

40

issue

1

eissn

1474-7596

issn

1474-760X

pii

10.1186/s13059-021-02268-4

journal_volume

22

pub_type

杂志文章

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