Abstract:
:Bone marrow stromal cells (MSCs) are a useful source of stem cells for the treatment of various brain injury diseases due to their abundant supply and fewer ethical problems compared with transplant treatment. However, the clinical application of MSCs is limited due to allograft rejection and immunosuppression in the process of MSCs transplantation. According to previous studies, microglial cell autophagy occurs following co-culture with MSCs. In the present study, exosomes were obtained from MSCs and subsequently characterized using transmission electron microscopy, atomic force microscopy and dynamic light scattering particle size analysis. The type of microRNAs (miRs) found in the exosomes was then analyzed via gene chip. The results demonstrated that microglial cell autophagy could be induced by exosomes. This mechanism was therefore investigated further via reverse transcription-quantitative PCR, western blotting and luciferase assays. These results demonstrated that exosomes from MSCs could induce microglial cell autophagy through the miR-32-mediated regulation of disabled homolog 2-interacting protein, thus providing a theoretical basis for the clinical application of miRs in MSCs.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Yuan FY,Zhang MX,Shi YH,Li MH,Ou JY,Bai WF,Zhang MSdoi
10.3892/etm.2020.9008subject
Has Abstractpub_date
2020-09-01 00:00:00pages
2752-2764issue
3eissn
1792-0981issn
1792-1015pii
ETM-0-0-9008journal_volume
20pub_type
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